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| 1 | Helicobacter pylori and corpus gastric pathology are associated with lower serum ghrelin显示文摘AIM To evaluate the association of Helicobacter pylori(H. pylori), cag A genotype, and type of gastric pathology with ghrelin, leptin and nutritional status.METHODS Fasted dyspeptic adults(18-70 years) referred for an upper digestive endoscopy were enrolled in this crosssectional study. Height and weight were assessed for body mass index(BMI) calculation. A sociodemographic survey was administered and nutrient intake was evaluated with 24 h dietary recalls. Serum total ghrelin and leptin levels were analyzed by enzymelinked immunosorbent assay. 13 C-Urea Breath Test was performed and four gastric biopsies were obtained during endoscopy for histopathology and H. pylori DNA amplification and genotyping. Data analysis was performed using χ2, Mann-Whitney U, Kruskal-Wallis tests, Spearman's correlation and linear regression.RESULTS One hundred and sixty-three patients(40.8 ± 14.0 years), 98/65 females/males, were included. Overall, persistent H. pylori prevalence was 53.4%(95%CI: 45.7%-65.8%). Neither nutrient intake nor BMI differed significantly between H. pylori positive and negative groups. Serum ghrelin was significantly lower in infected patients [median 311.0 pg/m L(IQR 230.0-385.5)] than in uninfected ones [median 355.0 pg/m L(IQR 253.8-547.8)](P = 0.025), even after adjusting for BMI and gender(P = 0.03). Ghrelin levels tended to be lower in patients carrying cag A positive strains both in the antrum and the corpus; however, differences with those carrying cag A negative strains did not reach statistical significance(P = 0.50 and P = 0.49, respectively). In addition, the type and severity of gastric pathology in the corpus was associated with lower serum ghrelin(P = 0.04), independently of H.pylori status. Conversely, leptin levels did not differ significantly between infected and uninfected patients [median 1.84 ng/m L(0.80-4.85) vs 1.84 ng/m L(0.50-5.09),(P = 0.51)]. CONCLUSION H. pylori infection and severity of gastric corpus pathology are associated with lower serum ghrelin. Further studies could confirm a lower ghrelin prevalence in cag A-positive patients. | Paula Mantero Gonzalo Sebastián Matus Rodolfo Ernesto Corti Ana María Cabanne Gerardo Gabriel Zerbetto de Palma Liliana Marchesi Olid María Marta Piskorz Marcela Beatriz Zubillaga Mariana Andrea Janjetic Cinthia Gabriela Goldman | 2018 | World Journal of Gastroenterology2018，24，3： | 8 |
| 2 | Oral probiotic VSL #3 attenuates the circulatory disturbances of patients with cirrhosis and ascites显示文摘 | Diego Rincón Javier Vaquero Ana Hernando Evelyn Galindo Cristina Ripoll Marta Puerto Magdalena Salcedo Rubén Francés Ana Matilla María V. Catalina Gerardo Clemente José Such Rafael Ba?ares | 2014 | Liver Int2014，，10： | 2 |
| 3 | Determination of fat, protein, and total solids in ovine milk by near-infrared spectroscopy显示文摘 | ELENA A PALOMA C GERARDO C | 1999 | Journal of AOAC International1999，82，3： | 1 |
| 4 | Aero- monas hydrophila clinical and environmental ecotypes as revealed by genetic diversity and virulence genes 显示文摘 | MaGuadalupe A A Cesar H R Gerardo Z | 2005 | FEMS Microbiol Lett2005，，10： | 1 |
| 5 | Dietary antioxidants:im- munity and host defense 显示文摘 | Maria A P Elena P Gerardo A de C | 2011 | Current Topics in Medicinal Chemistry2011，11，14： | 1 |
| 6 | Probabilistic approach for determining Salmonella spp. and L. monocytogenes concentration in pork meat from presence/absence microbiological data显示文摘 | Antonio Valero Marta Hernandez Alessandra De Cesare Gerardo Manfreda Rosa Maria García-Gimeno Patricia González-García David Rodríguez Lázaro | 2014 | International Journal of Food Microbiology2014，，： | 1 |
| 7 | Sodium Isopropyl Xanthate Degradation by Advanced Oxidation Processes显示文摘 | Gerardo Cifuentes Molina Cármen Herrera Cayo Marco Ant?nio Siqueira Rodrigues Andréa Moura Bernardes | 2012 | Minerals Engineering2012，，： | 1 |
| 8 | Functional properties and regulatory complexity of a minimal RBCS light-responsive unit activated by phytochrome,cryptochrome,and plastid signals显示文摘 | Alda M H Luisa L O Gerardo A A | 2002 | Plant Physiol2002，128，： | 1 |
| 9 | Two alternative modeling approaches for the evaluation of wind farm active and reactive power performances显示文摘 | Gerardo G Tapia A Ostolaza J X | 2006 | IEEE Trans on Energy Conversion2006，21，4： | 1 |
| 10 | Synthesis and characterization of polyaniline and polyaniline-Carbon nanotubes nanostructures for electrochemical supercapacitors显示文摘 | Marcela A Gerardo G Teresita Kessler | 2014 | Journal of Power Sources2014，56，245： | 1 |
| 11 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 12 | Low-resistivity Zn O∶F∶Al Transparent Thin Films显示文摘 | Delia C A Gerardo T D Sergio J S | 2004 | Solar Energy Material&Solar Cells2004，82，1： | 1 |
| 13 | Spanish-Latin American Multicenter Study of Attitudes Toward Organ Donation Among Personnel From Hospital Healthcare Centers显示文摘 | Antonio Ríos Ana López-Navas Marco Antonio Ayala-García María José Sebastián Anselmo Abdo-Cuza Jeannina Alán Laura Martínez-Alarcón Ector Jaime Ramírez Gerardo Mu?oz Juliette Suárez-López Roberto Castellanos Ricardo Ramírez Beatriz González Miguel Angel M | 2013 | Cirugía Espa?ola (English Edition)2013，，： | 1 |
| 14 | Retinal Microvascular Abnormalities and MRI-Defined Subclinical Cerebral Infarction: The Atherosclerosis Risk in Communities Study显示文摘 | Lawton S. Cooper Tien Y. Wong Ronald Klein A Richey Sharrett R Nick Bryan Larry D. Hubbard David J. Couper Gerardo Heiss Paul D. Sorlie | 2006 | Stroke2006，，1： | 1 |
| 15 | Induction of virulence factors, apoptosis, and cytokines in precision-cut hamster liver slices infected with Entamoeba histolytica显示文摘 | Pilar Carranza-Rosales María Guadalupe Santiago-Mauricio Nancy Elena Guzmán-Delgado Javier Vargas-Villarreal Gerardo Lozano-Garza Ezequiel Viveros-Valdez Rocío Ortiz-López Javier Morán-Martínez A. Jay Gandolfi | 2012 | Experimental Parasitology2012，，4： | 1 |
| 16 | Effect of adding insoluble solids from surimi wash water on the functional and mechanical properties of pacific whiting grade A surimi显示文摘 | Jose A Ramirez Gonzalo Velazquez Gerardo Lopez Echevarria | 2007 | Bioresource technology2007，98，11： | 1 |
| 17 | Effect of the number of parity on the main reproductive indicators of sows 显示文摘 | Gerardo 0 O Aureliano J A Antonio G V | 2014 | Revista Cientifica-Facultad De Ciencias Veterinarias2014，23，6： | 1 |
| 18 | The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa: differences from the zona pellucida显示文摘acrosome 反应(AR ) ,为精子的一个绝对要求和卵熔化,通过电压依赖者 Ca2+ 隧道和操作店的隧道要求 Ca2+ 的流入进精子。Maitotoxin (MTx ) ,一个动员 Ca2+ 代理人,被显示了是老鼠精子 AR 的有势力 inducer 与类似于带 pellucida (ZP ) 的药理学,可能为两 inducers 建议一条普通小径。使用的 recombinant 人 ZP3 (rhZP3 ) ,老鼠 ZP 和二 MTx 隧道 blockers (U73122 和 U73343 ) ,我们在人和老鼠精子调查了并且比较导致 MTx 、导致 ZP 的艺术。此处,我们报导 MTx 导致了 AR 并且提高了细胞内部的 Ca2+([Ca2+] 我) 在人的精子,哪个被 U73122 和 U73343 堵住。这二混合物也在老鼠精子禁止了导致 MTx 的 AR。在有我们的以前的建议的争论, AR 由 rhZP3 被触发或老鼠 ZP 没被 U73343 堵住,显示在人和老鼠精子,由生理的 ligands 或由 MTx 的 AR 正式就职通过不同小径发生了。U73122,然而并非 U73343 (不活跃的类似物) ,能堵住 phospholipase C (PLC ) 。另一个 PLC 禁止者, edelfosine,也堵住了导致 rhZP3 、导致 ZP 的艺术。这些调查结果在人和老鼠带证实了一条 PLC 依赖的发信号小径的参予导致蛋白质的 AR。尤其是, edelfosine 也禁止了导致 MTx 的老鼠精子 AR 然而并非人的,建议导致毒素的 AR 在老鼠是 PLC 依赖的并且在人 PLC 独立。 | lulio C Chaivez Gerardo A de Blas Josd L de la Vega-Beltran Takuya Nishigaki Mayel Chirinos Maria Elena Gonzaez-Gonzalez Fernando Larrea Alejandra Soiis Alberto Darszon Claudia L Trevino | 2011 | Asian Journal of Andrology2011，13，1： | 1 |
| 19 | Resource manipulation reveals flexible allocation rules to growth and reproduction in a Mediterranean evergreen oak显示文摘Aims In plants,resource allocation to growth and reproduction may depart from trade-off expectations if(i)investment in growth and reproduction relies on different resource pools,(ii)allocation to reproduction is dependent upon reaching some growth threshold or(iii)reproduction is developmentally linked to growth,both functions relying on the same resource pool.We examined the effects of enhanced resource level on patterns of resource allocation to growth and reproduction in holm oak(Quercus ilex sbsp.ballota),a Mediterranean evergreen tree.Methods In the experimental year(2003),we manipulated the amount of soil nutrients in autumn(to increase nutrient uptake during shoot elongation in the following spring)and soil water in summer(to increase water uptake during acorn growth).Indicators of growth and male and female reproduction were estimated in the preexperimental(2002),experimental(2003)and post-experimental(2004)years.Important Findings Fertilized trees produced significantly longer shoots,but the number of female flowers per shoot was not affected by treatments.The production of male catkins was also enhanced by fertilization.Irrigation did not affect the production of female flowers or abortion rates.Growth and female reproduction showed no consistent relationship in untreated trees,but resource addition elicited a growth-female reproduction trade-off in the experimental year.The sign of this significant relationship changed in the post-experimental year,indicating the existence of lagged effects of resource manipulation on acorn production.Overall,patterns of allocation to growth and reproduction varied as a function of sex,resource availability and year,a result consistent with extreme allocational plasticity in holm oak. | Fernando Pulido Gerardo Moreno Eustolia García José J.Obrador Raúl Bonal Mario Díaz | 2014 | Journal of Plant Ecology2014，7，1： | 1 |
| 20 | Removal of coloured compounds from sugar solution by adsorption onto anionic resins显示文摘 | Jorga A Carcia Agudo Maria Teresa Garcia Cubero Gerardo Gonzalez Benito | 2002 | Separation and Purification Technology2002，29，： | 1 |
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