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| 1 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 2 | The spatial and temporal distribution of microalgae in the South China Sea:evidence from GIS-based analysis of 18S rDNA sequences显示文摘The purpose of this study was to estimate the spatial and temporal variation of microalgae in the South China Sea and to demonstrate the environmental factors controlling the diversity of microalgae by GIS (geographic information system)-based analysis of 18S rDNA sequences. Six 18S rDNA libraries were constructed from environmental samples collected at different sites in the study area, and more than 600 18S rDNA sequences were determined. The rDNA sequence data were then analyzed by DIVA-GIS software to display the spatial and temporal variation of phytoplankton’s composition. It was shown that the autotrophic eukaryotic plankton dominated over the heterotrophic cells in most of our clone libraries, and the dominating phytoplankton was Dinophyceae except for Bacillariophyta at the Xiamen harbor. The percentages of these two groups were controlled by water temperature and salinity. Our results also revealed that the species composition of Chlorophyta showed a close relationship with latitude, changing from Prasinophyceae at the high latitude to Trebouxiophyceae at the low latitude. Several newly classified picoplankton lineages were first uncovered in the South China Sea, including the pico-sized green alga Ostreococcus sp. and Picochlorum eukaryotum, and picobiliphytes, which was just discovered in 2007 with unknown affinities to other eukaryotes. Their spatial and temporal variation were also analyzed and discussed. | LI LüYan1, HUANG QiaoJuan1, WU ShuHui1, LIN Duan2, CHEN JiaHui2 & CHEN YueQin1 1 Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Sun Yat-Sen University, Guangzhou 510275, China 2 South China Sea Environmental Monitoring Center, State Oceanic Administration, Guangzhou 510300,China | 2008 | Science China(Life Sciences)2008，51，12： | 2 |
| 3 | Detection of masked hypertension by home blood pressure measurement: is the number of measurements an important issue? 显示文摘 | Mallion JM Genes N Vaur L | 2004 | Blood Press Monit2004，9，6： | 1 |
| 4 | The Relation Between NOx Emissions and Precipitation NO3-in the Eastern USA显示文摘 | Thomas J B Gene L Francoise M | 2003 | Atmospheric Environment2003，37，： | 1 |
| 5 | coli cytoplasm 显示文摘 | LAVALLIE ER DIBLASIO EA KOVACIC S l A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E | 1993 | Biotechn ology1993，11，2： | 1 |
| 6 | Enhancement of LTP in aged rats is dependent on endogenous BDNF显示文摘 | Diógenes M J Costenla A R Lopes L V | 2011 | Neuropsychopharmacology2011，36，9： | 1 |
| 7 | 显示文摘 | Rahman M McVetty P B E,Li G Y,Development of SRAP1SNP and multiplexed SCAR molecular markers for the major seed coat color gene in Brassica rapa L | 2007 | Theoretical and Applied Genetics2007，115，8： | 1 |
| 8 | International Protection of Intellectual Property 显示文摘 | Grossman Gene M Edwin L - C Lai | 2004 | The American Economic Review2004，94，5： | 1 |
| 9 | Cadmium toxicity among wildlife in the Colorado Rocky Mountaion显示文摘 | James R L Gene E L John W F | | 0，，13： | 1 |
| 10 | Use of electronic pillboxes to assess risk of poor treatment compliance: results of a large-scale trial 显示文摘 | Vaur L Vaisse B Genes N | 1999 | Am J Hypertens1999，12，41： | 1 |
| 11 | Electronic pill-boxes in the evaluation of antihypertensive treatment compliance: comparison of once daily versus twice daily regimen 显示文摘 | Andrejak M Genes N Vaur L | 2000 | Am J Hypertens2000，13，2： | 1 |
| 12 | Numerical method for flu-id-structure interaction-A review显示文摘 | Gene H Jin W Anita L | 2012 | Commun Com-put Phys2012，1，2： | 1 |
| 13 | Embrologic and anatomic basis of inguinal herniorrhaphy显示文摘 | John E skandalakis Gene L | 1993 | Sury clin North Am1993，73，4： | 1 |
| 14 | Single nucleotide polymorphism its associations with chicken 显示文摘 | Ouyang J H Xie L Nie Q (SNP) at the GHR gene and growth and fat deposition traits (2): 87-95 | 2008 | Brit Pouh Sci2008，49，： | 1 |
| 15 | Prediction and confidence intervals for nonlinear measurement error models without identifiability information显示文摘 | Huwang L Gene H J T | 2002 | Statistics and Probability Letters2002，58，4： | 1 |
| 16 | A comparative clinical study of short-term results of laparoscopic surgery for rectal cancer during the learning curve显示文摘 | Ivan Arteaga González Hermógenes Díaz Luis Antonio Martín Malagón Eudaldo M. López-Tomassetti Fernández Javier Arranz Duran ángel Carrillo Pallares | 2006 | International Journal of Colorectal Disease2006，，6： | 1 |
| 17 | Phylogenetic analysis of the hyperthermophilic pink Filament community in octopus spring,Yellowstone National Park 显示文摘 | Reysenbach A L Gene S W Norman R P | 1994 | ApplEaviron Microbioi1994，60，： | 1 |
| 18 | Electronic pill boxes inthe evaluation of antihypertensive treatment compliance:Comparison of once daily versus twice daily regimen 显示文摘 | AndrejakM Genes N Vaur L | 2013 | Am J Hypertens2013，13，2： | 1 |
| 19 | Treatment of acute myocardial infarction by primary coronary angioplasty or intravenous thrombosis in the 'real world': one year result from a nationwide French survey显示文摘 | DANCHIN N VAUR L GENES N | 1999 | Circulation1999，99，： | 1 |
| 20 | Examining the Effects of Community Satisfaction and Attachment on Individual Well-Being 显示文摘 | | 2001 | Rural Sociology2001，66，4： | 1 |
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