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| 1 | Mucosal bacterial microflora and mucus layer thickness in adolescents with inflammatory bowel disease显示文摘AIM:To assess the mucosa-associated bacterial microflora and mucus layer in adolescents with inflammatory bowel disease(IBD) .METHODS:Sixty-one adolescents(mean age 15 years,SD ± 4.13) were included in the study.Intestinal biopsies from inflamed and non-inflamed mucosa of IBD patients and from controls with functional abdominal pain were cultured under aerobic and anaerobic conditions.The number of microbes belonging to the same group was calculated per weight of collected tissue.The mucus thickness in frozen samples was measured under a fluorescent microscope.RESULTS:The ratios of different bacterial groups in inflamed and non-inflamed mucosa of IBD patients and controls were specific for particular diseases.Streptococcus spp.were predominant in the inflamed mucosa of Crohn's disease(CD) patients(80% of all bacteria) ,and Lactobacillus spp.were predominant in ulcerative colitis patients(90%) .The differences were statistically significant(P = 0.01-0.001) .Lower number of bifidobacteria was observed in the whole IBD group.A relation was also found between clinical and endoscopic severity and decreased numbers of Lactobacillus and,to a lesser extent,of Streptococcus in biopsies from CD patients.The mucus layer in the inflamed sites was significantly thinner as compared to controls(P = 0.0033) and to non-inflamed areas in IBD patients(P = 0.031) .CONCLUSION:The significantly thinner mucosa of IBD patients showed a predominance of some aerobes specific for particular diseases,their numbers decreased in relation to higher clinical and endoscopic activity of the disease. | Krzysztof Fyderek Magdalena Strus Kinga Kowalska-Duplaga Tomasz Gosiewski Andrzej Wedrychowicz Urszula Jedynak-Wasowicz Malgorzata Sladek Stanislaw Pieczarkowski Pawel Adamski Piotr Kochan Piotr B Heczko | 2009 | World Journal of Gastroenterology2009，15，42： | 14 |
| 2 | Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease显示文摘AIM: To determine the features of Enterococcus that contribute to the development and maintenance of the inflammatory process in patients with inflammatory bowel disease (IBD). METHODS: Multiplex polymerase chain reaction (PCR) was applied to assess the presence of genes that encode virulence factors [surface aggregating protein (asa1), gelatinase (gelE), cytolysin (cylA), extracellular surface protein (esp) and hyaluronidase (hyl)] in the genomic DNA of 28 strains of Enterococcus isolated from the intestinal tissues of children with IBD (n =16) and of children without IBD (controls; n = 12). Additionally, strains with confirmed presence of the gelE gene were tested by PCR for the presence of quorum sensing genes (fsrA, fsrB, fsrC) that control the gelatinase production. Gelatinase activity was tested on agar plates containing 1.6% gelatin. We also analysed the ability of Enterococcus strains to release and decompose hydrogen peroxide (using Analytical Merckoquant peroxide test strips) and tested their ability to adhere to Caco-2 human gut epithelium cells and form biofilms in vitro. RESULTS: A comparison of the genomes of Enterococcus strains isolated from the inflamed mucosa of patients with IBD with those of the control group showed statistically significant differences in the frequency of theasa1 gene and thegelE gene. Furthermore, the cumulative occurrence of different virulence genes in the genome of a single strain ofEnterococcus isolated from the IBD patient group is greater than in a strain from the control group, although no significant difference was found. Statistically significant differences in the decomposition of hydrogen peroxide and adherence to the Caco-2 epithelial cell line between the strains from the patient group and control group were demonstrated. The results also showed that profuse biofilm production was more frequent amongEnterococcus strains isolated from children with IBD than in control strains. CONCLUSION: Enterococcus strains that adhere strongly to the intestinal epithelium, form biofilms and possess antioxidant defence mechanisms seem to have the greatest influence on the inflammatory process. | Edyta Golińska Anna Tomusiak Tomasz Gosiewski Gra yna Wiecek Agnieszka Machul Diana Mikoajczyk Magorzata Bulanda Piotr B Heczko Magdalena Strus | 2013 | World Journal of Gastroenterology2013，19，23： | 8 |
| 3 | Kinetics for thermal oxidation of lean methane-air gas mixtures in reverse-flow reactors 显示文摘 | GOSIEWSKI K WARMUZINSKI K JASCHIK M et al | 2007 | Chemical and Process Engineering2007，，28： | 1 |
| 4 | Efficiency of heat recovery versus maximum catalyst temperature in the reverse-flow combustion of methane显示文摘 | GOSIEWSKI K | 2005 | Chemical Engineering Journal2005，，107： | 1 |
| 5 | Effect of the mode of heat withdrawal on the asymmetry of temperature profiles in reverse-flow reactors显示文摘 | GOSIEWSKI K WARMUZINSKI K | 2007 | Chemical Engineering Science2007，，62： | 1 |
| 6 | Effective approach to cyclic steady state in the catalytic reverse-flow combustion of methane显示文摘 | GOSIEWSKI KRZYSZTOF | 2004 | Chemical Engineering Science2004，59，19： | 1 |
| 7 | Catalytic or thermal reversed flow combustion of coal mine ventilation air methane: What is better choice and when 显示文摘 | Gosiewski K Pawlaczyk A | 2014 | Chemical Engineering Journal2014，238，： | 1 |
| 8 | Energy recovery from ventilation air methane via reverse-flow reactors 显示文摘 | Gosiewski K Pawlaczyk A Jaschik M | 2015 | Energy2015，，： | 1 |
| 9 | A study on thermal combus-tion of lean methane-air mixtures:simplified reaction mechanism andkinetic equations显示文摘 | Krzysztof Gosiewski Anna Pawlaczyk | 2009 | Chemical Engineering Journal2009，154，： | 1 |
| 10 | Effect of the mode of heat withdrawal on the asymmetry of temperature profiles in reverse-flow reactors 显示文摘 | Gosiewski Krzysztof Warmuzinski Krzysztof | 2007 | Chemical Engineer- ing Science2007，62，： | 1 |
| 11 | A study on thermal combustion of lean methane-air mixtures: simplified reaction mechanism and kinetic equations显示文摘 | GOSIEWSKI K PAWLAVZYK A WARMUZINSKI K | 2009 | Chemical Engineering Journal2009，154，123： | 1 |
| 12 | Automatic balancing of flexible rotors, partⅠ: theoretical background 显示文摘 | Gosiewski Z | 1985 | Journal of Sound and Vibration1985，100，4： | 1 |
| 13 | Automatic balancing of flexible rotors, part Ⅱ : synthesis of system 显示文摘 | Gosiewski Z | 1987 | Journal of Sound and Vibration1987，114，1： | 1 |
| 14 | Effective Approach to Cyclic Steady State in the Catalytic Reverse-Flow Combustion of Methane 显示文摘 | Gosiewski K | 2004 | Chemical Engineering Science2004，59，19： | 1 |
| 15 | Effect of the mode of heat withdrawal on the asymmetry of temperature profiles in reverse-flow reactors: catalytic combustion of methane as a test case显示文摘 | Gosiewski K Warmuzinski K | 2007 | Chemical Engineering Science2007，62，10： | 1 |
| 16 | Efficiency of heat recovery versus maximum cat- alyst temperature in a reverse-flow combustion of methane 显示文摘 | Krzysztof Gosiewski | 2005 | Chemical Engineering Journal2005，107，13： | 1 |
| 17 | Effect of the mode of heat withdrawal on the asymmetry of temperature profiles in reverse-flow reactors, catalytic combustion of methane as a test case显示文摘 | GOSIEWSKI KRZYSZTOF WARMUZINSKI KRZYSZTOF | 2007 | Chemical Engineering Science2007，62，10： | 1 |
| 18 | Automatic Balancing of Flexible Rotors, Part I :Theoretical Background 显示文摘 | GOSIEWSKI Z | 1985 | Journal of Sound and Vibration1985，，100： | 1 |
| 19 | Automatic Balancing of Flexible Rotors, Part II:Synthesis of System 显示文摘 | GOSIEWSKI Z | 1987 | Journal of Sound and Vibration1987，，114： | 1 |
| 20 | Robust Control of Active Magnetic Suspension Analytical and Experimental Results显示文摘 | Gosiewski Zdzislaw Mystkowski Arkadiusz | 2008 | Mechanical Systems and Signal Processing2008，22，6： | 1 |
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