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| 1 | Microfluidic actuators based on temperature-responsive hydrogels显示文摘The concept of using stimuli-responsive hydrogels to actuate fluids in microfluidic devices is particularly attractive,but limitations,in terms of spatial resolution,speed,reliability and integration,have hindered its development during the past two decades.By patterning and grafting poly(N-isopropylacrylamide)PNIPAM hydrogel films on plane substrates with a 2μm horizontal resolution and closing the system afterward,we have succeeded in unblocking bottlenecks that thermo-sensitive hydrogel technology has been challenged with until now.In this paper,we demonstrate,for the first time with this technology,devices with up to 7800 actuated micro-cages that sequester and release solutes,along with valves actuated individually with closing and opening switching times of 0.6±0.1 and 0.25±0.15 s,respectively.Two applications of this technology are illustrated in the domain of single cell handling and the nuclear acid amplification test(NAAT)for the Human Synaptojanin 1 gene,which is suspected to be involved in several neurodegenerative diseases such as Parkinson’s disease.The performance of the temperature-responsive hydrogels we demonstrate here suggests that in association with their moderate costs,hydrogels may represent an alternative to the actuation or handling techniques currently used in microfluidics,that are,pressure actuated polydimethylsiloxane(PDMS)valves and droplets. | Loïc D'Eramo Benjamin Chollet Marie Leman Ekkachai Martwong Mengxing Li Hubert Geisler Jules Dupire Margaux Kerdraon Clémence Vergne Fabrice Monti Yvette Tran Patrick Tabeling | 2018 | Microsystems & Nanoengineering2018，4，1： | 3 |
| 2 | Multichannel texture analysis using localized spatial filters显示文摘 | Bovic A C Clark C Geisler W S | 1990 | IEEE Transactions on Pattern Analysis and Machine Intelligence1990，12，1： | 2 |
| 3 | Multichannel Texture Analysis Using Localized Spatial Filters 显示文摘 | BOVIK A C CLARK M GEISLER W S | 1990 | IEEE Transaction on Pattern Analysis and Machine Intel ligence1990，12，1： | 1 |
| 4 | Early but not late stentthrombosis is influenced by residual platelet aggregation in patientsundergoing coronary interventions显示文摘 | Geisler T Zürn C Simonenko R | 2010 | Eur Heart J2010，31，1： | 1 |
| 5 | Olfactory event-related potentials and aging: normative data 显示文摘 | Murphy C Morgan CD Geisler MW | 2000 | Int J Psychophysiol2000，36，： | 1 |
| 6 | Recovery of motor function after spinal cord injury--a randomized,placebo-controlled trial with GM-1 ganglioside显示文摘 | GEISLER F H DORSEY F C COLEMAN W P | | 0，，26： | 1 |
| 7 | Identification of genes encoding N-glycan processing beta-Nacetylglucosaminidases in Trichoplusia ni and Bombyx mori: Implications for glycoengineering of baculovirus expression systems显示文摘 | GEISLER C JARVIS D L | 2010 | Biotechnol Prog2010，26，1： | 1 |
| 8 | p16 and p53 protein expression as prognostic indicators of survival and disease re- currence from head and neck cancer显示文摘 | Geisler S A Olshan A F Weissler M C | 2002 | Clin Cancer Res2002，8，11： | 1 |
| 9 | Laparoscopic exp loration for the clinically undetected hernia in infancy and childhood显示文摘 | Geisler D P Jegathesan S Parmley M C | 2001 | Am J Surg2001，182，6： | 1 |
| 10 | Arthroscopic locking plate removal af- ter proximal humeral fractures 显示文摘 | Voigt C Geisler A Lill H | 2010 | Arch Orthop Trauma Surg2010，130，3： | 1 |
| 11 | ALS3 encodes a phloem-localized ABC transporter-like protein that is required for aluminum tolerance in Arabidopsis 显示文摘 | Larsen P B Geisler M J B Jones C A | 2005 | The Plant Journal2005，41，3： | 1 |
| 12 | Robotically assisted total laparoscopic radical trachelectomy for fertility sparing in stage ⅠB1 adenosarcoma of the cervix显示文摘 | GEISLER J P ORR C J MANAHAN K J | 2008 | J Laparoendosc Adv Surg Tech A2008，18，5： | 1 |
| 13 | Long-term molecular remissions in patients with indolent lymphoma treated with rituximab as a single agent or in combination with interferon alpha-2a: A randomized phase Ⅱ study from the Nordic Lymphoma Group显示文摘 | Kimby E Jurlander J Geisler C | 2008 | Leuk Lymphoma2008，49，1： | 1 |
| 14 | Factors affecting recombi?nant Western equine encephalitis virus glycoprotein production in the baculovirus eystem显示文摘 | Toth AM Geisler C Aumiller JJ | 2011 | Protein Expr Purif2011，80，2： | 1 |
| 15 | Revisability of the CHAIRTE artificial disc replacement:analysis of 347 patients enrolled in the US IDE study of the Charite Artificial Disc显示文摘 | McAfee P C Geisler F H Saiedy SS | 2006 | Spine2006，31，11： | 1 |
| 16 | 显示文摘 | Geisler M Braatz C Bruch J Kastner A Kress M Ruske M Willms T Zmehy A | 2003 | Thin Solid Film2003，442，： | 1 |
| 17 | A cochlear model using feed-forward outer-hair-cell forces 显示文摘 | Geisler C D Sang C | 1995 | Hearing Research1995，86，13： | 1 |
| 18 | Multichannel texture analysis using localized spatial filters 显示文摘 | Bovik A C Clark M Geisler W S | 1990 | IEEE Transactions on Pattern Analysis and Machine Intelligence1990，12，1： | 1 |
| 19 | Olfactory event-related potentials and aging: normative data显示文摘 | MURPHY C MORGAN C D GEISLER M W | 2000 | Int J Psychophysiol2000，36，： | 1 |
| 20 | MicroRNAs in mantle cell lymphoma显示文摘 | Husby S Geisler C Gronbaek K | 2013 | Leuk Lymphoma2013，54，9： | 1 |
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