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| 1 | Measurement of calprotectin in ascitic fluid to identify elevated polymorphonuclear cell count显示文摘AIM: To evaluate the diagnostic capability of calprotectin in ascitic fluid for detecting a polymorphonuclear (PMN) cell count > 250/μL ascites. METHODS: In this prospective observational study, a total of 130 ascites samples were analysed from 71 consecutive patients referred for paracentesis. Total and differential leukocyte cell counts were determined manually with a Neubauer chamber and gentianviolet stain. Calprotectin was measured in 1 mL ascetic fluid by enzyme-linked immunosorbent assay (ELISA) and a point-of-care (POC) lateral flow assay with the Quantum Blue Reader (Bühlmann Laboratories). All measurements were carried out in a central laboratory by senior personnel blinded to patient history. A PMN count > 250/μL was the primary endpoint of the study. The diagnostic value of ascitic calprotectin measurement was assessed by comparing to the final diagnosis of each patient that had been adjudicated by investigators blinded to calprotectin values. RESULTS: The PMN count was > 250/μL in 19 samples (14.6%) from 15 patients (21.1%) and varied widely among the study population (range 10-19 800/mL and 1-17 820/mL, respectively). Spontaneous bacterial peritonitis (SBP) was the final diagnosis in four patients (5.6%). All patients with PMN ≤ 250/μL had negative bacterial culture. PMN count was elevated in five patients with peritoneal carcinomatosis, three with lymphoma, one with neuroendocrine carcinoma, and two with secondary peritonitis due to abdominal perforation. PMN cell counts correlated with ascitic calprotectin values (Spearman's rho; r = 0.457 for ELISA, r = 0.473 for POC). A considerable range of ascitic calprotectin concentrations was detected by ELISA [median 0.43 μg/mL, interquartile range (IQR) 0.23-1.23 (range 0.10-14.93)] and POC [median 0.38 μg/mL, IQR 0.38-0.56 (range 0.38-13.31)]. Ascitic calprotectin levels were higher in samples with PMN > 250/μL, by both ELISA [median (IQR) 2.48 μg/mL (1.61-3.65) vs 0.10 μg/mL (0.10-0.36), P < 0.001] and POC [2.78 μg/mL (2.05-5.37) vs 0.38 μg/mL (0.38-0.41), P < 0.001]. The area under the receiver operating characteristics curve for identifying an elevated PMN count was 0.977 (95%CI: 0.933 to 0.995) for ELISA and 0.982 (95%CI: 0.942 to 0.997) for POC (P = 0.246 vs ELISA). Using the optimal cut-off value for ELISA (0.63 μg/mL), ascitic calprotectin had 94.8% sensitivity, 89.2% specificity, positive and negative likelihood ratios of 8.76 and 0.06 respectively, positive and negative predictive values of 60.0% and 99.0% respectively, and 90.0% overall accuracy. Using the optimal cut-off value for POC (0.51 μg/mL), the respective values were 100.0%, 84.7%, 6.53, 0.00, 52.8%, 100% and 87.7%. Correlation between ELISA and POC was excellent (r = 0.873, P < 0.001). The mean ± SD of the difference was -0.11 ± 0.48 μg/mL with limits of agreement of + 0.8 μg/mL (95%CI: 0.69 to 0.98) and -1.1 μg/mL (95%CI: -1.19 to -0.91). CONCLUSION: Ascitic calprotectin reliably predicts PMN count > 250/μL, which may prove useful in the diagnosis of SBP, especially with a readily available bedside testing device. | Emanuel Burri Felix Schulte Jürgen Muser Rémy Meier Christoph Beglinger | 2013 | World Journal of Gastroenterology2013，19，13： | 10 |
| 2 | 离子型和非离子型碘造影剂的生物学安全性显示文摘 | Laniado M Felix R | 1988 | 国外医学:临床放射学分册1988，11，3： | 7 |
| 3 | Galvanically induced potentials to enable minimal tribochemical wear of stainless steel lubricated with sodium chloride and ionic liquid aqueous solution显示文摘The effect of galvanically induced potentials on the friction and wear behavior of a 1 RK91 stainless steel regarding to tribocorrosion was investigated using an oscillating ball-on-disk tribometer equipped with an electrochemical cell. The aim of this investigation is to develop a water-based lubricant. Therefore 1 molar sodium chloride(NaCl) and 1% 1-ethyl-3-methylimidazolium chloride [C_2 mim][Cl] water solutions were used. Tribological performance at two galvanically induced potentials was compared with the non-polarized state: cathodic potential-coupling with pure aluminum- and anodic potential-coupling with pure copper. Frictional and electrochemical response was recorded during the tests. In addition, wear morphology and chemical composition of the steel were analyzed using scanning electron microscopy(SEM) and X-ray photoelectron spectroscopy(XPS), respectively. The galvanically induced cathodic polarization of the stainless steel surface results in electrochemical corrosion protection and the formation of a tribolayer. Cations from the electrolyte(sodium Na^+ and 1-ethyl- 3-methylimidazolium [C_2 mim]^+) interact and adhere on the surface. These chemical interactions lead to considerably reduced wear using 1 NaC l(86%) and 1% 1-ethyl-3-methylimidazolium chloride [C_2 mim][Cl](74%) compared to the nonpolarized system. In addition, mechanical and corrosive part of wear was identified using this electrochemical technique. Therefore this method describes a promising method to develop water-based lubricants for technical applications. | Tobias AMANN Felix GATTI Natalie OBERLE Andreas KAILER Jürgen RüHE | 2018 | Friction2018，6，2： | 7 |
| 4 | 成人IDH野生型星形细胞瘤从生物学和临床上归为其他肿瘤类型显示文摘WHO III级的IDH野生型间变性星形细胞瘤预后差。为了对星形细胞瘤进行分子亚组分型或诊断上的重新分类,作者分析160例成人IDH野生型星形细胞肿瘤的分子标志性变异,并行甲基化和拷贝数分析,其中包括120例 WHO III级的间变性星形细胞瘤和40例WHO II级的弥漫型星形细胞瘤。基于分子谱系和标志性变异特征,将这些肿瘤分为四个主要亚型。78%肿瘤诊断为传统胶质母细胞瘤分子亚型。9%为GBM-H3F3A突变(GBM-H3)亚型。8%具有独特的甲基化特征,与GBM-H3-K27相似,但缺乏H3F3A突变,这组肿瘤主要发生在幕下及中线部位,并且预后较好。120例IDH野生型的间变性星形细胞瘤除1例外均可归入上述3种亚型中。40例WHO II级的弥漫型星形细胞瘤中7例具有不同的分子特征,其中6例重新归类为其他不同的低级别肿瘤类型。有趣的是,TERT突变只发生在分子亚型的GBM (78%),且预后不良。 GBM-H3组缺乏 TERT突变,然而其预后更差。 | David E R Annekathrin K Felix S 李晓玲 王行富 | 2015 | 临床与实验病理学杂志2015，31，9： | 4 |
| 5 | Hyperthermia in combined treatment of cancer显示文摘 | P Wust B Hildebrandt G Sreenivasa B Rau J Gellermann H Riess R Felix PM Schlag | 2002 | Lancet Oncology2002，，8： | 2 |
| 6 | Pancreatic cancer cells surviving gemcitabine treatment express markersof stem cell differentiation and epithelial-mesenchymal transition显示文摘 | Karl Quint Manuel Tonigold Pietro Di Fazio Roberta Montalbano Susanne Lingelbach Felix Rückert Beate Alinger Matthias Ocker Daniel Neureiter | 2012 | International Journal of Oncology2012，，6： | 2 |
| 7 | Some radar imagery results using supper resolution technique显示文摘 | EMANUEL R FELIX COSTINEL T | 2004 | IEEE Trans on Antennas and Propagation2004，52，5： | 1 |
| 8 | A method for the determination of volatile N-nitrosamines in food by HS-SPME-GC-TEA 显示文摘 | Raquel A Felix G R Susanne R | 2005 | Food Chemistry2005，91，1： | 1 |
| 9 | Effect of bisphosphonates on proliferation and viability of mouse bone marrow-derives macrophages显示文摘 | Cecchini MG Felix R Fleisch H | 1987 | J Bone Miner Res1987，2，2： | 1 |
| 10 | Prediction of metabolic syndrome by low serum testosterone levels in men:results from the study of health in Pomerania显示文摘 | Haring R Volzke H Felix SB | 2009 | Diabetes2009，58，9： | 1 |
| 11 | Molecular physiology and pathology of Ca^2 + -conducting channels in the plasma membrane of mananalian sperm显示文摘 | Felix R | 2005 | Reproduction2005，129，3： | 1 |
| 12 | Effect of a Secretolytic and a Combination of Pinene, Limonene and Cineole on Mucociliary Clearance in Patients with Chronic Pulmonary Obstruction显示文摘 | Dorow P Weiss Th Felix R | 1987 | Arzneim Forsch1987，37，： | 1 |
| 13 | Numerical approaches to treatment planning in deep RF-hyperthermia 显示文摘 | Wust P Nadobny J Felix R | 1989 | Strahlentherapie und Onkologie1989，165，75： | 1 |
| 14 | Fuzzy constraint networks for signal pattern recognition 显示文摘 | Felix P Barro S Maryyn R | 2003 | Artificial Intelligence Special Issue on Fuzzy Logic2003，148，12： | 1 |
| 15 | Associations between hepatocyte growth factor, c-Met, and basic fibroblast growth factor and survival in endometrial cancer patients显示文摘 | Felix A S Edwards R P Stone R A | 2012 | Br J Cancer2012，106，12： | 1 |
| 16 | Challenge of Swiss Arbitral Awards- se- lected Statistical Data as 2013显示文摘 | FELIX D DAVID R | 2014 | ASA Bulletin2014，，32： | 1 |
| 17 | Comparison of survival out- comes between patients with malignant mixed mullerian tumors and high-grade endometrioid, clear cell, and papillary serous endometri- al cancers 显示文摘 | Felix AS Stone RA Bowser R | 2011 | Int J Gynecol Cancer2011，21，： | 1 |
| 18 | Use of theophylline in chronic obstructive pulmonaly disease: examining the evidence 显示文摘 | FELIX S R | 2006 | Curr Opin Inpulm Med2006，12，： | 1 |
| 19 | Dissection of functional domains of the voltage-dependent Ca^2+ channel alpha2 delta subunit显示文摘 | FELIX R GURNETT CA DE WAARD M | 1997 | J Neurosci1997，17，18： | 1 |
| 20 | Magnetic resonance imaging of orbital tumors显示文摘 | Lemke A J Kazi I Felix R | 2006 | Eur Radiol2006，16，： | 1 |
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