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| 1 | miRNAs and lncRNAs in vascular injury and remodeling显示文摘Vascular injury,remodeling,as well as angiogenesis,are the leading causes of coronary or cerebrovascular disease.The blood vessel functional imbalance trends to induce atherosclerosis,hypertension,and pulmonary arterial hypertension.As several genes have been identified to be dynamically regulated during vascular injury and remodeling,it is becoming widely accepted that several types of non-coding RNA,such as microRNAs(miRNAs)and long non-coding RNAs(lncRNAs),are involved in regulating the endothelial cell and vascular smooth muscle cell(VSMC)behaviors.Here,we review the progress of the extant studies on mechanistic,clinical and diagnostic implications of miRNAs and lncRNAs in vascular injury and remodeling,as well as angiogenesis,emphasizing the important roles of miRNAs and lncRNAs in vascular diseases.Furthermore,we introduce the interaction between miRNAs and lncRNAs,and highlight the mechanism through which lncRNAs are regulating the miRNA function.We envisage that continuous in-depth research of non-coding RNAs in vascular disease will have significant implications for the treatment of coronary or cerebrovascular diseases. | SONG XiaoWei SHAN DongKai CHEN Jian JING Qing | 2014 | Science China(Life Sciences)2014，57，8： | 17 |
| 2 | GNSS-R data acquisition system design and experiment显示文摘In this paper,the design of a GNSS-R data acquisition system is described,including the antenna,RF(radio frequency)front-end and FPGA(field programmable gate array)based logic circuit for data storage interface.Two RF front-ends are connected with the RHCP(right-hand circular polarization)and LHCP(left-hand circular polarization)antenna to collect the direct and reflected signal separately.Both the specifications and the circuit connections are given in detail.Experiments are performed for statics at the seashore and dynamic scenario in the sea using airplanes.The results of the data analysis show that the proposed data acquisition system is suitable for GNSS-R primitive data collection. | YANG DongKai WANG Yan LU Yong LI WeiQiang LI ZiWei | 2010 | Chinese Science Bulletin2010，55，33： | 9 |
| 3 | Exosome-based small RNA delivery:Progress and prospects显示文摘RNA interfering(RNAi), mediated by small interfering RNAs and microRNAs, is currently one of the most promising tools of gene therapy. Small RNAs are capable of inducing specific post-transcriptional gene silencing, providing a potentially effective platform for the treatment of a wide array of diseases. However, similar to other nucleic acid-based drugs,the major hurdle of RNAi therapy is lack of efficient and non-immunogenic delivery vehicles. Currently, viruses, synthetic polymers, and lipid-based carriers are among the most widely studied vehicles for small RNA delivery. However, many drawbacks are reported to be associated with these delivery vehicles. There is a pressing need to replace them with more efficient and better-tolerated approaches. Exosomes secreted from the endocytic compartment of live cells, are a subtype of endogenous extracellular vesicles that transfer genetic and biochemical information among different cells, thus playing an important role in cellcell communication. Recently, accumulating attention has been focused on harnessing exosomes as nanaocarriers for small RNAs delivery. Due to their natural role in shuttling endogenous nucleic acid in our body, exosomes may exhibit higher delivery efficiency, lower immunogenicity, and better compatibility than existing foreign RNA carriers. Importantly,exosomes own intrinsic homing capacity that can guide small RNAs across natural membranous barriers. Moreover, such a capacity can be further improved by adding appropriate targeting moieties. In this manuscript, we briefly review the progress and challenges of RNAi therapy, and discuss the potential of exosomes' applications in small RNA delivery with focus on the most recent advances in exosome-based small RNA delivery for disease therapy. | Mei Lu Haonan Xing Zhe Xun Tianzhi Yang Pingtian Ding Cuifang Cai Dongkai Wang Xiaoyun Zhao | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，1： | 8 |
| 4 | The role and clinical implications of microRNAs in hepatocellular carcinoma显示文摘Hepatocellular carcinoma (HCC) is common and one of the most aggressive of all human cancers. Recent studies have indicated that miRNAs, a class of small noncoding RNAs that regulate gene expression post-transcriptionally, directly contribute to HCC by targeting many critical regulatory genes. Several miRNAs are involved in hepatitis B or hepatitis C virus replication and virus-induced changes, whereas others participate in multiple intracellular signaling pathways that modulate apoptosis, cell cycle checkpoints, and growth-factor-stimulated responses. When disturbed, these pathways appear to result in malignant transformation and ultimately HCC development. Recently, miRNAs circulating in the blood have acted as possible early diagnostic markers for HCC. These miRNA also could serve as indicators with respect to drug efficacy and be prognostic in HCC patients. Such biomarkers would assist stratification of HCC patients and help direct personalized therapy. Here, we summarize recent advances regarding the role of miRNAs in HCC development and progression. Our expectation is that these and ongoing studies will contribute to the understanding of the multiple roles of these small noncoding RNAs in liver tumorigenesis. | ZHAO Xue YANG Zhen LI GuangBing LI DongKai ZHAO Yi WU Yan ROBSON Simon C. HE Lian XU YiYao MIAO R-uoYu ZHAO HaiTao | 2012 | Science China(Life Sciences)2012，55，10： | 6 |
| 5 | Familial Parkinson's Disease-Associated L166P Mutant DJ-1 is Cleaved by Mitochondrial Serine Protease Omi/HtrA2显示文摘Parkinson's disease(PD) is the most common neurodegenerative movement disorder. Mutations in the DJ-1, including L166 P, are responsible for recessive earlyonset PD. Many lines of evidence have shown that L166 P is not only a loss-of-function mutant, but also a proapoptotic-like protein that results in mitochondrial dysfunction. L166 P has been reported to be unstable and to mislocalize to mitochondria. However, the mechanisms underlying the instability of L166 P compared to wild-type DJ-1 remain largely unknown. Here, we showed that Omi/Htr A2, a mitochondrial serine protease that has also been linked to the pathogenesis of PD, contributed to L166 P instability. Omi directly interacted with and cleaved L166 P in mitochondria to decrease the L166 P level. However,Omi did not bind and cleave wild-type DJ-1. Moreover,Omi cleaved L166 P at both serine residues 3 and 121,while L166 P-induced cell death under H_2O_2 treatment was alleviated by over-expression of Omi. Our data reveal a bridge between DJ-1 and Omi, two PD-associated geneticfactors, which contributes to our understanding of the pathogenesis of PD. | Kai Fu Yanfei Wang Dongkai Guo Guanghui Wang Haigang Ren | 2017 | Neuroscience Bulletin2017，33，6： | 6 |
| 6 | Cholesterol-tuned liposomal membrane rigidity directs tumor penetration and anti-tumor effect显示文摘Recently, liposomes have been widely used in cancer therapeutics, but their anti-tumor effects are suboptimal due to limited tumor penetration. To solve this problem, researchers have made significant efforts to optimize liposomal diameters and potentials, but little attention has been paid to liposomal membrane rigidity. Herein, we sought to demonstrate the effects of cholesterol-tuned liposomal membrane rigidity on tumor penetration and anti-tumor effects. In this study, liposomes composed of hydrogenated soybean phospholipids(HSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000](DSPE-PEG2000) and different concentrations of cholesterol were prepared. It was revealed that liposomal membrane rigidity decreased with the addition of cholesterol. Moderate cholesterol content conferred excellent diffusivity to liposomes in simulated diffusion medium, while excessive cholesterol limited the diffusion process. We concluded that the differences of the diffusion rates likely stemmed from the alterations in liposomal membrane rigidity, with moderate rigidity leading to improved diffusion. Next, the in vitro tumor penetration and the in vivo anti-tumor effects were analyzed. The results showed that liposomes with moderate rigidity gained excellent tumor penetration and enhanced anti-tumor effects. These findings illustrate a feasible and effective way to improve tumor penetration and therapeutic efficacy of liposomes by changing the cholesterol content, and highlight the importance of liposomal membrane rigidity. | Hangyi Wu Miaorong Yu Yunqiu Miao Shufang He Zhuo Dai Wenyi Song Yuan Liu Sha Song Ejaj Ahmad Dongkai Wang Yong Gan | 2019 | Acta Pharmaceutica Sinica B2019，9，4： | 5 |
| 7 | GPS Multipath Signal Estimation Based on All Phase DFT显示文摘 | YANG Dongkai YAO Yanxin GUO Jia | 2010 | Chinese Journal of Electronics2010，19，2： | 4 |
| 8 | Design, Synthesis and Herbicidal Activity of Novel Sulfonylureas Containing Tetrahydrophthalimide Substructure显示文摘 | HUA Xuewen ZHOU Sha CHEN Minggui ZHANG Dongkai LIU Ming LIU Jingbo WU Changchun LIU Yang LI Yonghong LI Zhengming | 2016 | Chemical Research in Chinese Universities2016，32，3： | 3 |
| 9 | A GPS Receiver Adaptive Digital Beamforming Interference Suppression Algorithm Based on Kalman Filter显示文摘 | DING Wenrui ZHU Yunlong ZHANG Bo YANG Dongkai | 2013 | Chinese Journal of Electronics2013，22，2： | 3 |
| 10 | Simulation of Multi-satellite GNSS Reflected Signals and Design of Simulator显示文摘Using GNSS-R technology for remote sensing of surface parameters has become a new trend in the field of remote sensing.With the rapid development of GNSS-R technology,GNSS-R simulation has become one of the new hot spots.Now the researches of the GNSS-R simulation are all the simulations that consider a single star or a single frequency point,and in actual applications,the signal captured by the receiver is often the reflected signals of multiple stars.In view of this situation,from the perspective of multi-satellite simulation,this paper gives the model of GNSS-R multi-satellite ocean simulation on the basis of analyzing the remote sensing principle,reflection signal model and simulation principle of GNSS-R technology.Based on the GNSS-R multi-satellite ocean simulation model and the fast parallel computing capability of GPU,the GNSS-R multi-satellite ocean simulator was designed.Finally,the direct and reflected signals generated by the GNSS-R multi-satellite simulator were tested and verified.The results show that the positioning result of the direct signal meets the positioning accuracy requirements;The delay-related power results obtained from the simulated two-satellite reflected signals processing are in good agreement with the theoretical model,and the correlation coefficients are all above 0.99;The generated signals are used for GNSS-R height measurement technology,the height measurement error is about 1.4~1.8 m,which is in line with the accuracy of the C/A code ranging receiver;And the parallel operation of the GPU for multi-satellite simulation calculation is 800—900 times higher than the traditional CPU calculation.It proves that the proposed model and the designed simulator are feasible and accurate. | Bowen LI Dongkai YANG Bo ZHANG | 2021 | Journal of Geodesy and Geoinformation Science2021，4，2： | 3 |
| 11 | Morphology-controllable synthesis and characterization of CeO_2 nanocrystals显示文摘CeO 2 nanocrystals with different morphologies were synthesized by adjusting the pH value of the starting solution in water-in-oil microemulsion.The obtained samples were characterized by transmission electron microscopy (TEM),X-ray diffraction (XRD),thermogravimetric analysis and different thermal analysis (TGA/DTA),Brunauer-Emmet-Teller (BET) surface area measurement,ultraviolet-visible absorption (UV-vis) spectroscopy and photoluminescence (PL) spectroscopy.Results show that the morphologies of CeO 2 were transformed from granular to spherical and to rod-like while the pH of the starting solution varied from 5 to 8 and to 11.All samples were indexed to the phase of CeO 2 and Ce(OH) 4,and the molar ratio of CeO 2 to Ce(OH) 4 was deduced to be about 0.25.The morphologies of CeO 2 nanocrystals had but slight influence on their specific surface areas,UV-vis spectra and PL spectra.The band gap energies of different morphological samples were estimated by UV-vis spectroscopic method. | Yangfeng Huang Yebin Cai Dongkai Qiao Hao Liu | 2011 | Particuology2011，9，2： | 3 |
| 12 | Molecular characterization and developmental expression patterns of thyroid hormone receptors(TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata显示文摘Considering some advantages of Rana nigromaculata as an experimental species, we propose that this species, like Xenopus laevis, could be used to assay thyroid hormone(TH) signaling disrupting actions. To validate the utilizability of R. nigromaculata, we investigated the responsiveness of R. nigromaculata to a TH receptor(TR) agonist(T3) and antagonist(amiodarone) by analyzing expression, based on characterizing TR cDNA and developmental expression patterns. With high levels of identity with the corresponding genes in X. laevis, both TRα and TRβ in R. nigromaculata exhibited roughly similar developmental expression patterns to those of X. laevis, in spite of some species-specific differences. Both TRα and TRβ expression had greater changes in the liver and intestine than in the tail and brain during metamorphosis. T3 exposure for 2 days induced more dramatic increases of TRβ expression in stage 27 than in stage34 tadpoles but not in stage 42 tadpoles, showing that the responsiveness of R. nigromaculata to TH decreased with development and disappeared at the onset of metamorphic climax.Corresponding to greater changes of TRβ expression in the liver and intestine than in the tail and brain during metamorphosis, the liver and intestine had higher responsiveness to exogenous T3 than the tail and brain. Amiodarone inhibited T3-induced TRβ expression. Our results show that R. nigromaculata can be used as a model species for assaying TH signaling disrupting actions by analyzing TRβ expression, and intestine tissues at stage 27 are ideal test materials due to high responsiveness and easy accessibility. | Qinqin Lou Yinfeng Zhang Dongkai Ren Haiming Xu Yaxian Zhao Zhanfen Qin Wuji Wei | 2014 | Journal of Environmental Sciences2014，26，10： | 3 |
| 13 | Standard pancreatoduodenectomy versus extended pancreatoduodenectomy with modified retroperitoneal nerve resection in patients with pancreatic head cancer:a multicenter randomized controlled trial显示文摘Background:The extent of pancreatoduodenectomy for pancreatic head cancer remains controversial,and more high-level clinical evidence is needed.This study aimed to evaluate the outcome of extended pancreatoduodenectomy(EPD)with retroperitoneal nerve resection in pancreatic head cancer.Methods:This multicenter randomized trial was performed at 6 Chinese highvolume hospitals that enrolled patients between October 3,2012,and September 21,2017.Four hundred patients with stage I or II pancreatic head cancer and without specific pancreatic cancer treatments(preoperative chemotherapy or chemoradiation)within three months were randomly assigned to undergo standard pancreatoduodenectomy(SPD)or EPD,with the latter followed by dissection of additional lymph nodes(LNs),nerves and soft tissues 270◦on the right side surrounding the superior mesenteric artery and celiac axis.The primary endpoint was overall survival(OS)by intention-to-treat(ITT).The secondary endpoints were disease-free survival(DFS),mortality,morbidity,and postoperative pain intensity.Results:TheR1 ratewas slightly lower with EPD(8.46%)thanwith SPD(12.56%).The morbidity and mortality rates were similar between the two groups.The median OS was similar in the EPD and SPD groups by ITT in the whole study cohort(23.0 vs.20.2 months,P=0.100),while the median DFS was superior in the EPD group(16.1 vs.13.2 months,P=0.031).Patients with preoperative CA19–9<200.0 U/mL had significantly improved OS and DFS with EPD(EPD vs.SPD,30.8 vs.20.9 months,P=0.009;23.4 vs.13.5 months,P<0.001).The EPD group exhibited significantly lower locoregional(16.48%vs.35.20%,P<0.001)andmesenteric LNrecurrence rates(3.98%vs.10.06%,P=0.022).The EPD group exhibited less back pain 6 months postoperation than the SPD group.Conclusions:EPD for pancreatic head cancer did not significantly improve OS,but patients with EPD treatment had significantly improved DFS.In the subgroup analysis,improvements in bothOS and DFS in the EPD armwere observed in patients with preoperative CA19–9<200.0 U/mL.EPD could be used as an effective surgical procedure for patients with pancreatic head cancer,especially those with preoperative CA19–9<200.0 U/mL. | Qing Lin Shangyou Zheng Xianjun Yu Meifu Chen Yu Zhou Quanbo Zhou Chonghui Hu Jing Gu Zhongdong Xu LinWang Yimin Liu Qingyu Liu MinWang Guolin Li He Cheng Dongkai Zhou Guodong Liu Zhiqiang Fu Yu Long Yixiong Li Weilin Wang Renyi Qin Zhihua Li Rufu Chen | 2023 | Cancer Communications2023，43，2： | 3 |
| 14 | Effects of Logging Intensity on Structure and Composition of a Broadleaf-Korean Pine Mixed Forest on Changbai Mountains,Northeast China显示文摘In order to identify a harvesting model which is beneficial for broadleaf-Korean pine mixed forest(BKF) sustainability, we investigated four types of harvested stands which have been logged with intensities of 0(T0, control), 15%(T1, low intensity), 35%(T2, moderate intensity), and 100%(T3, clear-cutting), and examined the impacts of logging intensity on composition and structure of these stands. Results showed that there were no significant differences between T0 and T1 for all structural characteristics, except for density of seeding and large trees. The mean diameter at breast height(DBH, 1.3 m above the ground), stem density and basal area of large trees in T2 were significantly lower than in T0, while the density of seedlings and saplings were significantly higher in T2 than in T0. Structural characteristics in T3 were entirely different from T0. Dominant tree species in primary BKF comprised 93%, 85%, 45% and 10% of the total basal area in T0, T1, T2 and T3, respectively. Three community similarity indices, the Jaccard′s similarity coefficient(CJ); the Morisita-Horn index(CMH); and the Bray-Curtis index(CN), were the highest for T0 and T1, followed by T0 and T2, and T0 and T3, in generally. These results suggest that effects of harvesting on forest composition and structure are related to logging intensities. Low intensity harvesting is conductive to preserving forest structure and composition, allowing it to recover in a short time period. The regime characterized by low logging intensity and short rotations appears to be a sustainable harvesting method for BKF on the Changbai Mountains. | WU Zhijun SU Dongkai NIU Lijun Bernard Joseph LEWIS YU Dapao ZHOU Li ZHOU Wangming WU Shengnan DAI Limin | 2016 | Chinese Geographical Science2016，26，1： | 2 |
| 15 | Notch Effect and Frequency Compensation of Dual-Sensor OBC Data in Shallow Water显示文摘Reverberation is significant in shallow water and produces obvious notches in OBC spectra.It also degrades the quality of sections and increases the difficulty of processing and interpretation.This article presents the relationship between notch,shooting depth,and seabed depth based on the seismic convolution model.Forward modelling based on wave equation theory is used to verify this relationship.Dual-sensor summation is applied to suppress receiver-side multiples and remove notches according to the opposite response of geophones and hydrophones to down-going wave fields based on a detailed analysis of the OBC technique.The good results obtained in practical applications reveal the effectiveness of this method. | Dongkai Wang Siyou Tong Huaishan Liu Weiqiang Zhu | 2015 | Journal of Earth Science2015，26，4： | 2 |
| 16 | Meiotic Chromosome Behavior in a Human Male t(8;15)Carrier显示文摘Reciprocal translocation is one of the most common structural chromosomal rearrangements in human beings;it is widely recognized to be associated with male infertility.This association is mainly based on the abnormal chromosome behavior of the translocated chromosomes and sex chromosomes during meiosis prophase I in reciprocal translocation carriers.However,the underlying mechanisms are not completely known.Here we report a reciprocal translocation carrier of t(8;15),who is oligozoospermic due to apoptosis of primary spermatocytes and to premature germ cell desquamation from seminiferous tubules.Further analysis showed abnormal synapsis and recombination frequency in this patient,indicating a connection between chromosome behavior and apoptosis of primary spermatocytes.We also compared these observations with recently reported findings on spermatogenesis defects in reciprocal translocation carriers,and discuss the possible mechanisms underlying bom common and unique phenotypes of reciprocal translocations involving different chromosomes with the aim of further understanding the regulation of human spermatogenesis. | Hanwei Jiang Liu Wang Yingxia Cui Zhipeng Xu Tonghang Guo Dongkai Cheng Peng Xu Wen Yu Qinghua Shi | 2014 | Journal of Genetics and Genomics2014，41，3： | 2 |
| 17 | Solder balling of lead-free solder pastes 显示文摘 | Minna Arra Dongkai Shangguan Ristolainen Eero | 2002 | J Electron Mater2002，31，11： | 1 |
| 18 | Global Existence of Solutions to Fuzzy Voherra Integral Equations显示文摘 | ZHANG Dongkai FENG Wenli QIU Jiqing | 2009 | ICIC Express Letters2009，3，3： | 1 |
| 19 | Dual-responsive nanovaccine for cytosolic delivery of antigens to boost cellular immune responses and cancer immunotherapy显示文摘Cancer vaccine contributing to the success of the treatment and prevention of tumors has attracted a huge attention as a strategy for tumor immunotherapy in recent years.A major challenge of cancer vaccine is to target cytosols of dendritic cells(DCs)in the lymph nodes(LNs)to enhance efficiency of antigen cross-presentation,which elicits high levels of cytotoxic T-lymphocytes to destruct tumor cells.Here,we address this issue by conjugating ovalbumin(OVA)to PEG-PCL using disulfide bond(-ss-),and the degradable pH-responsive polymer-PEI-PCL as delivery carrier.In addition,the mol ratio of PEG-PCL to PEI-PCL in the mixed micelles was tailored to deliver the OVA to LNs.Subsequently,CpG ODN_(1826),a TLR-9 agonist,was further introduced into a mixed micelle of 30nm or less as a unique tumor vaccine.Importantly,the results demonstrated the mixed micelles with 1:1mol of PCL-PEG and PCL-PEI can effectively migrate to distal LNs where antigen were efficiently captured by DCs,meanwhile,OVA was modified to the surface of mixed micelles via disulfide bonds(-ss-)for promotion efficiency of antigen cross-presentation.More surprisingly,combination of tumor vaccine with anti-PD-1,the therapy of ectopic melanoma(B16-OVA)and lung metastasis melanoma(B16-OVA)is excellent therapeutic effect.Taken together,our works offers a novel strategy for the cytosol delivery of antigens to achieve potent cancer immunotherapy. | Yang Sui Ji Li Jiqiang Qu Ting Fang Hongyan Zhang Jian Zhang Zheran Wang Mingyu Xia Yinghui Dai Dongkai Wang | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，4： | 1 |
| 20 | Design, Synthesis and Fungicidal Activity of Novel Strobilurin-l,2,4-Triazole Derivatives Containing Furan or Thiophene Rings显示文摘A series of strobilurin-l,2,4-triazole derivatives containing a furan or thiophen ring was designed andsynthesized. The structures of the compounds were characterized by 1H NMR, 13C NMR and HRMS spectra. Thebioassays indicated that the fungicidal activities of compounds 10b(EC50=14.82 mg/L) and 10c(EC50=18.72 mg/L)against Cercospora arachidicola Hori in vitro were much higher than that of Azoxystrobin as control(EC50=40.54mg/L) and the fungicidal activities of compounds 10u(EC50=8.66 rag/L) and 10n(EC50=9.89 rag/L) against Rhizotoniacerealis in vitro were higher than that of the same control(EC50=10.86 rag/L). Compounds 10b, 10c, 1011 and 10kcould be considered as the leading compounds for further investigation. | LIU Yang LIU Ming ZHANG Dongkai HUA Xuewen WANG Baolei ZHOU Sha LI Zhengming | 2016 | Chemical Research in Chinese Universities2016，32，6： | 1 |
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