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| 1 | Virus like particle-based vaccines against emerging infectious disease viruses显示文摘Emerging infectious diseases are major threats to human health.Most severe viral disease outbreaks occur in developing regions where health conditions are poor.With increased international travel and business,the possibility of eventually transmitting infectious viruses between different countries is increasing.The most effective approach in preventing viral diseases is vaccination.However,vaccines are not currently available for numerous viral diseases.Viruslike particles(VLPs) are engineered vaccine candidates that have been studied for decades.VLPs are constructed by viral protein expression in various expression systems that promote the selfassembly of proteins into structures resembling virus particles.VLPs have antigenicity similar to that of the native virus,but are non-infectious as they lack key viral genetic material.VLP vaccines have attracted considerable research interest because they offer several advantages over traditional vaccines.Studies have shown that VLP vaccines can stimulate both humoral and cellular immune responses,which may offer effective antiviral protection.Here we review recent developments with VLP-based vaccines for several highly virulent emerging or re-emerging infectious diseases.The infectious agents discussed include RNA viruses from different virus families,such as the Arenaviridae,Bunyaviridae,Caliciviridae,Coronaviridae,Filoviridae,Flaviviridae,Orthomyxoviridae,Paramyxoviridae,and Togaviridae families. | Jinliang Liu Shiyu Dai Manli Wang Zhihong Hu Hualin Wang Fei Deng | 2016 | Virologica Sinica2016，31，4： | 7 |
| 2 | Establishment of a Reverse Genetic System of Severe Fever with Thrombocytopenia Syndrome Virus Based on a C4 Strain显示文摘Severe fever with thrombocytopenia syndrome virus(SFTSV)is an emerging tick-borne bunyavirus that causes hemorrhagic fever-like disease(SFTS)in humans with a case fatality rate up to 30%.To date,the molecular biology involved in SFTSV infection remains obscure.There are seven major genotypes of SFTSV(C1-C4 and J1-J3)and previously a reverse genetic system was established on a C3 strain of SFTSV.Here,we reported successfully establishment of a reverse genetics system based on a SFTSV C4 strain.First,we obtained the 5’-and 3’-terminal untranslated region(UTR)sequences of the Large(L),Medium(M)and Small(S)segments of a laboratory-adapted SFTSV C4 strain through rapid amplification of cDNA ends analysis,and developed functional T7 polymerase-based L-,M-and S-segment minigenome assays.Then,fulllength cDNA clones were constructed and infectious SFTSV were recovered from co-transfected cells.Viral infectivity,growth kinetics,and viral protein expression profile of the rescued virus were compared with the laboratory-adapted virus.Focus formation assay showed that the size and morphology of the foci formed by the rescued SFTSV were indistinguishable with the laboratory-adapted virus.However,one-step growth curve and nucleoprotein expression analyses revealed the rescued virus replicated less efficiently than the laboratory-adapted virus.Sequence analysis indicated that the difference may be due to the mutations in the laboratory-adapted strain which are more prone to cell culture.The results help us to understand the molecular biology of SFTSV,and provide a useful tool for developing vaccines and antivirals against SFTS. | Mingyue Xu Bo Wang Fei Deng Hualin Wang Manli Wang Zhihong Hu Jia Liu | 2021 | Virologica Sinica2021，36，5： | 5 |
| 3 | Viral suppression of innate immunity via spatial isolation of TBK1/IKKε from mitochondrial antivirai platform显示文摘 | Yun-Jia Ning Manli Wang Maping Deng Shu Shen Wei Liu Wu-Chun Cao Fei Deng Yan-Yi Wang Zhihong Hu Hualin Wang | 2014 | Journal of Molecular Cell Biology2014，8，4： | 4 |
| 4 | Characterization of two monoclonal antibodies, 38F10 and 44D11, against the major envelope fusion protein of Helicoverpa armigera nucleopolyhedrovirus显示文摘The envelope fusion protein F of baculoviruses is a class I viral fusion protein which play a significant role during virus entry into insect cells. F is initially synthesized as a precursor(F_0) and then cleaved into a disulfide-linked F_1 and F_2 subunits during the process of protein maturation and secretion. To facilitate further investigation into the structure and function of F protein during virus infection, monoclonal antibodies(mAbs) against the F_2 subunit of Helicoverpa armigera nucleopolyhedrovirus(HearNPV)(Ha F) were generated. Two kinds of mAbs were obtained according to their different recognition epitopes: one kind of mAbs, as represented by 38F10,recognizes amino acid(aa) 85 to 123 of F_2 and the other kind, represented by 44D11, recognizes aa148 to 173 of F_2. Western blot and immunofluorescence assay confirmed that both of the mAbs recognized the F protein expressed in HearNPV infected cells, however, only 44D11 could neutralize HearNPV infection. The results further showed that 44D11 may not interact with a receptor binding epitope, rather it was demonstrated to inhibit syncytium formation in cells expressing the Ha F protein. The results imply that the monoclonal antibody 44D11 recognizes a region within HaF_2 that may be involved in the F-mediated membrane fusion process. | Zijiao Zou Jinliang Liu Zhiying Wang Fei Deng HuaUn Wang Zhihong Hu Manli Wang Tao Zhang | 2016 | Virologica Sinica2016，31，6： | 3 |
| 5 | Improving Baculovirus Transduction of Mammalian Cells by Incorporation of Thogotovirus Glycoproteins显示文摘Baculovirus can transduce a wide range of mammalian cells and is considered a promising gene therapy vector. However,the low transduction efficiency of baculovirus into many mammalian cells limits its practical application. Co-expressing heterologous viral glycoproteins(GPs), such as vesicular stomatitis virus G protein(VSV G), with baculovirus native envelope protein GP64 is one of the feasible strategies for improving virus transduction. Tick-borne thogotoviruses infect mammals and their GPs share sequence/structure homology and common evolutionary origins with baculovirus GP64.Herein, we tested whether thogotovirus GPs could facilitate the entry of the prototype baculovirus Autographa californica multiple multiple nucleopolyhedrovirus(AcMNPV) into mammalian cells. The gp genes of two thogotoviruses, Thogoto virus and Dhori virus, were inserted into the AcMNPV genome. Both GPs were properly expressed and incorporated into the envelope of the recombinant AcMNPVs. The transduction rates of recombinant AcMNPVs expressing the two thogotovirus GPs increased for approximately 4–12 fold compared to the wild type AcMNPV in six of the 12 tested mammalian cell lines. It seemed that thogotovirus GPs provide the recombinant AcMNPVs with different cell tropisms and showed better performance in several mammalian cells compared to VSV G incorporated AcMNPV. Further studies showed that the improved transduction was a result of augmented virus-endosome fusion and endosome escaping, rather than increased cell binding or internalization. We found the AcMNPV envelope protein GP64-mediated fusion was enhanced by the thogotovirus GPs at relatively higher p H conditions. Therefore, the thogotovirus GPs represent novel candidates to improve baculovirus-based gene delivery vectors. | Liangbo Hu Yimeng Li Fei Deng Zhihong Hu Hualin Wang Manli Wang | 2019 | Virologica Sinica2019，34，4： | 3 |
| 6 | Effects of inorganic and organic salts on aggregation behavior of cationic gemini surfactants 显示文摘 | YU DEFENG HUANG XU DENG MANLI | 2010 | J Phys Chem B2010，114，14： | 1 |
| 7 | Assembly of layer-by-layer films of superoxide dismutase and gold nanorods:A third generation biosensor for superoxide anion显示文摘Based on the layer-by-layer self-assembly of positively charged cetyltrimethylammonium bromide (CTAB) wrapped gold na- norods (AuNRs) and negatively charged superoxide dismutase (SOD) from their aqueous solutions on cysteine modified gold electrode (Cys/Au), a third generation electrochemical biosensor ((SOD/AuNRs)2/Cys/Au) for superoxide anion (O2·-) was developed. The two layers assembly of SOD/AuNRs can significantly enhance the direct electron transfer between SOD and the electrode. The functional enzymatic activities of the SOD offer an electrochemical approach to the determination of O2·-. In the reductive regions, the proposed sensor exhibits excellent analytical performances, such as wide linear range (200 nM to 0.2 mM O2·-), low detection limit (100 nM O2·-), high sensitivity (22.11 nA cm-2 μM-1), short response time (less than 5 s), good stability and reproducibility, while no obvious interferences are caused by commonly met interfering species including hydrogen peroxide (H2O2), uric acid (UA) and ascorbic acid (AA). | WANG MengDong HAN YiTao LIU XingXing NIE Zhou DENG ChunYan GUO ManLi YAO ShouZhuo | 2011 | Science China Chemistry2011，54，8： | 1 |
| 8 | Investigation on rapid degradation of sodium dodecyl benzene sulfonate(SDBS) under microwave irradiation in the presence of modified activated carbon powder with ferreous sulfate 显示文摘 | ZHANG Zhaohong DENG Yingqiao SHEN Manli | 2009 | Desalination2009，249，12： | 1 |
| 9 | Coacervation of cat ionic Gemini surfactant with weakly charged anionic polyac- rylamide显示文摘 | Deng Manli Cao Meiwen Wang Yilin | 2009 | The Journal of Physical Chemistry B2009，113，28： | 1 |
| 10 | Investigation on Rapid Degradation of Sodium Dodccyl Benzene Sulfonate (SDBS) Under Microwave Irradiation in the Pres~lce of Modified Activated Carbon Powder with Ferreous Sulfate 显示文摘 | Zhang Zhaohong Deng Yingqiao Sben Manli | 2009 | Desalination2009，249，3： | 1 |
| 11 | P33 of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus is a functional homolog of AcP33显示文摘Dear Editor,Baculoviruses are insect-specific viruses with a circular double-stranded DNA genome ranging in size from80–180 kb(Lu et al.,2012).Two distinct types of virions have been identified during the infectious cycle of baculoviruses,namely budded virions(BVs)and occlusion-derived virions(ODVs).BVs mediate infection from cell to cell,while ODVs initiate oral infection in the insect midgut(Braunagel and Summers,2007). | Wenhua Kuang Huanyu Zhang Dianhai Hou Manli Wang Fei Deng Hualin Wang Zhihong Hu | 2016 | Virologica Sinica2016，31，4： | 1 |
| 12 | Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China显示文摘Apis mellifera filamentous virus(Am FV)is a large DNA virus that is endemic in honeybee colonies.The genome sequence of the Am FV Swiss isolate(Am FV CH–C05)has been reported,but so far very few molecular studies have been conducted on this virus.In this study,we isolated and purified Am FV(Am FV CN)from Chinese honeybee(Apis mellifera)colonies and elucidated its genomics and proteomics.Electron microscopy showed ovoid purified virions with dimensions of 300–500×210–285 nm,wrapping a 3165×40 nm filamentous nucleocapsid in three figure-eight loops.Unlike Am FV CH–C05,which was reported to have a circular genome,our data suggest that Am FV CN has a linear genome of approximately 493 kb.A total of 197 ORFs were identified,among which36 putative genes including 18 baculoviral homologs were annotated.The overall nucleotide similarity between the CN and CH–C05 isolates was 96.9%.Several ORFs were newly annotated in Am FV CN,including homologs of per os infectivity factor 4(PIF4)and a putative integrase.Phylogenomic analysis placed Am FVs on a separate branch within the newly proposed virus class Naldaviricetes.Proteomic analysis revealed 47 Am FV virionassociated proteins,of which 14 had over 50%sequence coverage,suggesting that they are likely to be main structural proteins.In addition,all six of the annotated PIFs(PIF-0–5)were identified by proteomics,suggesting that they may function as entry factors in Am FV infection.This study provides fundamental information regarding the molecular biology of Am FV. | Dahe Yang Jun Wang Xi Wang Fei Deng Qingyun Diao Manli Wang Zhihong Hu Chunsheng Hou | 2022 | Virologica Sinica2022，37，4： | 1 |
| 13 | Comparative Studies on Preparation of Large Plant DNA Fragments by Pulse Field Gel Electrophoresis显示文摘The preparation of large plant DNA fragments is extremely important to the construction of large insert DNA libraries (YAC, BAC, PAC and TAC). Although several techniques have been developed in each step of large plant DNA fragments preparation, the whole processing remains complicated and difficult. Based on authors research experience and the recent worldwide development in this field, the following aspects are discussed in this paper: techniques of plant high molecular weight (HMW) DNA purification by pre-electrophoresis, the optimal conditions for the partial digestion of the HMW DNA by HindIII, the isolation effects of of large plant DNA fragments (100~400 kb) with different parameters of pulse field gel electrophoresis (PFGE), and the recovery of large DNA fragments. Through comparative studies, the advantages and disadvantages of each technique are discussed and some recommendations are proposed for preparing high quality large plant DNA fragments. The suggested techniques have been used in preparing the large DNA fragments of maize, rice, moss, laver, sea tangle and peach,and similar results are obtained among all the materials. This paper only reports the results using maize as material. | 杨继良 王庆华 Deng Daiyong Yang Dianer JIN Demin WENG Manli ZHANG Juren Wang Bin | 2004 | High Technology Letters2004，10，1： | 1 |
| 14 | Hybridoma-derived neutralizing monoclonal antibodies against Beta and Delta variants of SARS-CoV-2 in vivo显示文摘Neutralizing monoclonal antibodies(mAb)are a major therapeutic strategy for the treatment of severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)infection.The continuous emergence of new SARS-CoV-2 variants worldwide has increased the urgency for the development of new mAbs.In this study,we immunized mice with the receptor-binding domain(RBD)of the SARS-CoV-2 prototypic strain(WIV04)and screened 35 RBDspecific mAbs using hybridoma technology.Results of the plaque reduction neutralization test showed that 25 of the mAbs neutralized authentic WIV04 strain infection.The 25 mAbs were divided into three categories based on the competitive enzyme-linked immunosorbent assay results.A representative mAb was selected from each category(RD4,RD10,and RD14)to determine the binding kinetics and median inhibitory concentration(IC_(50))of WIV04 and two variants of concern(VOC):B.1.351(Beta)and B.1.617.2(Delta).RD4 neutralized the B.1.617.2 variant with an IC50 of 2.67 ng/mL;however,it completely lost neutralizing activity against the B.1.351 variant.RD10 neutralized both variants with an IC50 exceeding 100 ng/mL;whereas RD14 neutralized two variants with a higher IC50(>1 mg/mL).Animal experiments were performed to evaluate the protective effects of RD4 and RD10 against various VOC infections.RD4 could protect Adv-hACE2 transduced mice from B.1.617.2 infection at an antibody concentration of 25 mg/kg,while RD10 could protect mice from B.1.351 infection at an antibody concentration of 75 mg/kg.These results highlight the potential for future modifications of the mAbs for practical use. | Qianran Wang Lu Peng Yanqiu Nie Yanni Shu Huajun Zhang Zidan Song Yufeng Li Hengrui Hu Liushuai Li Xi Wang Jia Liu Jiang Li Zhengli Shi Fei Deng Yu Guo Yiwu Zhou Bing Yan Zhihong Hu Manli Wang | 2023 | Virologica Sinica2023，38，2： | 0 |
| 15 | Functional Characterization of the Group Ⅰ Alphabaculovirus Specific Gene ac73显示文摘Baculoviridae is a family of large DNA viruses that specifically infect insects.It contains four genera,Alpha-,Beta-,Gamma-,and Deltabaculovirus.Alphabaculovirus is further divided into Group I and II,and Group I appears to be emerged most recently among all baculoviruses.Interestingly,there are 12 Group I specific genes that are only found in this lineage.Studying these genes is helpful to understand how baculoviruses evolved.Here,we reported the functional analyzing results of ac73y a function unknown Group I specific gene of Autographa califomica multiple nucleopolyhedrovirus(AcMNPV)which is the type species of baculovirus.The AC73 protein encoded by ac73 was found to be expressed during the late stage of infection and incorporated into the nucleocapsids of budded virus(BV)and occlusionderived virus(ODV).In infected cells,AC73 resided mainly in the ring zone region of the nucleus,and appeared to be assembled into occlusion bodies(OBs).The ac73 knockout and repaired viruses were constructed and studied by in vitro and in vivo infection.Although ac73 was not essential for BV and ODV or OB formation,the BV titer and viral infectivity in insect larvae of ac73 knockout AcMNPV decreased by about 5-8 and 3-4 fold compared to those of wild type virus,respectively,suggesting ac73 contributed to infectious BV production and viral infectivity in vivo.This research provides new insight into the function of this Group I specific gene. | Wei Shao Lihong He Qingxiu Chen Jiang Li Fei Deng Hualin Wang Zhihong Hu Manli Wang | 2019 | Virologica Sinica2019，34，6： | 0 |
| 16 | Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group Ⅰ of Alphabaculovirus显示文摘The Cyclophragma undans nucleopolyhedrovirus(Cyun NPV), a potential pest control agent, was isolated from Cyclophragma undans(Lepidoptera: Lasiocampidae), an important forest pest. In the present study, we performed detailed genome analysis of Cyun NPV and compared its genome to those of other Group Ⅰ alphabaculoviruses. Sequencing of the Cyun NPV genome using the Roche 454 sequencing system generated 142,900 bp with a G + C content of 45%. Genome analysis predicted a total of 147 hypothetical open reading frames comprising 38 baculoviral core genes, 24 lepidopteran baculovirus conserved genes, nine Group Ⅰ Alphabaculovirus conserved genes, 71 common genes, and five genes that are unique to Cyun NPV. In addition, the genome contains 13 homologous repeated sequences(hrs). Phylogenetic analysis groups Cyun NPV under a distinct branch within clade ‘‘a'' of Group Ⅰ in the genus Alphabaculovirus. Unlike other members of Group Ⅰ, Cyun NPV harbors only nine of the 11 genes previously determined to be specific to Group Ⅰ viruses.Furthermore, the Cyun NPV lacks the tyrosine phosphatase gene and the ac30 gene. The Cyun NPV F-like protein contains two insertions of continuous polar amino acids, one at the conventional fusion peptide and a second insertion at the pretransmembrane domain. The insertions are likely to affect the fusion function and suggest an evolutionary process that led to inactivation of the F-like protein. The above findings imply that Cyun NPV is a distinct species under Group Ⅰ Alphabaculovirus. | Zheng Zhu Jun Wang Qianran Wang Feifei Yin Xiaoping Liu Dianhai Hou Lei Zhang Haizhou Liu Jiang Li Basil M. Arif Hualin Wang Fei Deng Zhihong Hu Manli Wang | 2018 | Virologica Sinica2018，33，4： | 0 |
| 17 | HearNPV Pseudotyped with PIF1,2,and 3 from MabrNPV:Infectivity and Complex Stability显示文摘Effective oral infection is set off by interaction of a group of conserved per os infectivity factors(PIFs) with larval midgut columnar epithelial cells. We constructed pseudotyped viruses by substituting pif1, pif2 or pif3 genes of Helicoverpa armigera nucleopolyhedrovirus(Hear NPV) with their homologs from Mamestra bracissae multiple nucleopolyhedrovirus and tested their infectivity to tissue culture cells and to larvae. Transfection and infection assays revealed that all recombinant viruses generated infectious budded virus in both cell culture and in larvae. Electron microscopy showed synthesized occlusion body and occlusion derived virus(ODV) were morphologically indistinguishable from those of the parental virus. By contrast, feeding assays revealed that pseudotyped viruses could not rescue oral infectivity except for pif3 pseudotyped virus that only partially rescued oral infectivity but at a mortality rate much lower than that of the parental Hear NPV. Consistent with the bioassay result, PIF complex was detected in ODVs of pif3 pseudotyped virus only but not in pif1 or pif2 pseudotyped viruses. Our results suggest that PIF complex is essential for oral infectivity, and in the formation of the PIF complex, PIF1, 2 are virus-specific while PIF3 does not appear to be as specific and can function in heterologous environment, albeit to a much more limited extent. | George Alliwa Makalliwa Xi Wang Huanyu Zhang Nan Zhang Cheng Chen Jiang Li Fei Deng Hualin Wang Manli Wang Zhihong Hu | 2018 | Virologica Sinica2018，33，2： | 0 |
| 18 | Construction and Characterization of a Novel Bacmid AcBac-Syn Based on a Synthesized Baculovirus Genome显示文摘Baculoviruses are large DNA viruses which have been widely used as expression vectors and biological insecticides.Homologous recombination and Bac-to-Bac system have been the main methods for manipulating the baculovirus genome.Recently, we generated a synthetic baculovirus Ac MNPV-WIV-Syn1 which fully resembled its parental virus Autographa californica multiple nucleopolyhedrovirus(Ac MNPV). Here, we report the modification of Ac MNPV-WIV-Syn1 into a novel bacmid, Ac Bac-Syn, which can be used as a backbone for Bac-to-Bac system. To achieve this, a vector contained a Lac Z:att Tn7 and egfp cassette was constructed, and recombined with a linearized Ac MNPV-WIV-Syn1 genome by transformation-associated recombination in yeast to generate bacmid Ac Bac-Syn. The bacmid was then transfected to insect cells and the rescued virus showed similar biological characteristics to the wild-type virus in terms of the kinetics of budded virus production, the morphology of occlusion bodies, and the oral infectivity in insect larvae. For demonstration, a red fluorescent protein gene Dsred was transposed into the att Tn7 site by conventional Bac-to-Bac method, and the transfection and infection assays showed that Ac Bac-Syn can be readily used for foreign gene insertion and expression.Ac Bac-Syn has several advantages over the conventional Ac MNPV bacmids, such as it contains an egfp reporter gene which facilitates visualization of virus propagation and titration;its DNA copy numbers could be induced to a higher level in E. coli;and the retaining of the native polyhedrin gene in the genome making it an attractive system for studying the functions of gene related to occlusion body assembly and oral infection. | Yu Shang Hengrui Hu Xi Wang Hualin Wang Fei Deng Manli Wang Zhihong Hu | 2021 | Virologica Sinica2021，36，6： | 0 |
| 19 | Correction to: Establishment of a Reverse Genetic System of Severe Fever with Thrombocytopenia Syndrome Virus Based on a C4 Strain显示文摘Correction to:Virologica Sinica http://gffzzd3cc09b8251d45dfsxvvpw90w0upn6u9n.ffgz.tsg.suse.edu.cn/10.1007/s12250-021-00359-x The Original Article was published on 15 March 2021.Due to our negligence,the affiliation of author Mingyue Xu was mislabeled.The affiliation footnotes in the byline for Mingyue Xu should read as given below.We apologized for these errors. | Mingyue Xu Bo Wang Fei Deng Hualin Wang Manli Wang Zhihong Hu Jia Liu | 2021 | Virologica Sinica2021，36，6： | 0 |
| 20 | Comparative Antiviral Efficacy of Viral Protease Inhibitors against the Novel SARS-CoV-2 In Vitro显示文摘The recent outbreak of novel coronavirus pneumonia(COVID-19)caused by a new coronavirus has posed a great threat to public health.Identifying safe and effective antivirals is of urgent demand to cure the huge number of patients.Virusencoded proteases are considered potential drug targets.The human immunodeficiency virus protease inhibitors(lopinavir/ritonavir)has been recommended in the global Solidarity Trial in March launched by World Health Organization.However,there is currently no experimental evidence to support or against its clinical use.We evaluated the antiviral efficacy of lopinavir/ritonavir along with other two viral protease inhibitors in vitro,and discussed the possible inhibitory mechanism in silico.The in vitro to in vivo extrapolation was carried out to assess whether lopinavir/ritonavir could be effective in clinical.Among the four tested compounds,lopinavir showed the best inhibitory effect against the novel coronavirus infection.However,further in vitro to in vivo extrapolation of pharmacokinetics suggested that lopinavir/ritonavir could not reach effective concentration under standard dosing regimen[marketed as Kaletraò,contained lopinavir/ritonavir(200 mg/50 mg)tablets,recommended dosage is 400 mg/10 mg(2 tablets)twice daily].This research concluded that lopinavir/ritonavir should be stopped for clinical use due to the huge gap between in vitro IC50 and free plasma concentration.Nevertheless,the structure–activity relationship analysis of the four inhibitors provided further information for de novel design of future viral protease inhibitors of SARS-CoV-2. | Leike Zhang Jia Liu Ruiyuan Cao Mingyue Xu Yan Wu Weijuan Shang Xi Wang Huanyu Zhang Xiaming Jiang Yuan Sun Hengrui Hu Yufeng Li Gang Zou Min Zhang Lei Zhao Wei Li Xiaojia Guo Xiaomei Zhuang Xing-Lou Yang Zheng-Li Shi Fei Deng Zhihong Hu Gengfu Xiao Manli Wang Wu Zhong | 2020 | Virologica Sinica2020，35，6： | 0 |
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