| 1 | 早期反应基因-1(Egr-1)在缺血再灌注肝脏组织中的表达显示文摘目的研究缺血再灌注后肝组织中早期反应基因-1(Egr-1)的表达。方法动脉放血至平均动脉压(MAP)25 mmHg,分别维持1 h或2.5 h,复制小鼠整体肝缺血模型;2.5 h后,回输失血+2倍于失血量的平衡液至MAP 80 mmHg,复制小鼠肝缺血再灌注模型。取肝组织,分别通过Northern印迹法、凝胶电泳迁移分析(EMSA)、蛋白质免疫印迹法,检测肝组织mRNA的表达;肝核提取物中Egr-1蛋白与DNA的结合活性;肝组织、胞浆及核提取物中Egr-1蛋白的表达。结果缺血1 h后,Egr-1mRNA在肝组织中的表达明显增强;缺血2.5 h后肝组织中Egr-1mRNA虽有所下降,但表达水平仍较高。缺血2.5 h+再灌注4 h后,肝组织中Egr-1mRNA消失。缺血2.5 h和缺血2.5 h+再灌注4 h后肝细胞核提取物中Egr-1蛋白的DNA结合活性增加。缺血2.5 h及缺血2.5 h+再灌注4 h肝组织及肝细胞核提取物中Egr-1蛋白表达明显增加;Egr-1蛋白仅在缺血2.5 h肝胞浆提取物中表达。结论本实验结果表明肝缺血再灌注后Egr-1mRNA和蛋白表达均明显增强,其DNA结合活性增加,说明其转录和翻译水平均增加。本研究显示缺血再灌注后肝脏中Egr-1基因被激活,Egr-1可能参与了肝I/R后炎症反应基因的调节,并在肝损伤中起一定的作用。 | 梅建民 Ti mothy R.Billiar 吕民生 David J.Gallo 杨荣华 聂洪峰 | 2005 | 中华肝胆外科杂志2005，11，11： | 3 |
| 5 | Accelerated neutral atom beam(ANAB)and gas clustered ion beam(GCIB)treatment of implantable device polymers leads to decreased bacterial attachment in vitro and decreased inflammation in vivo显示文摘Infections at the placement site of biomaterial-based devices and subsequent scar formation results in morbidity,which may require revision surgery.Biomaterials intended for permanent implantation in the body need to be biologically inert to avoid excessive foreign body response and to reduce bacterial attachment.In this study,we show that polymeric materials commonly used in medical devices,including polyetheretherketone(PEEK)and polypropylene,treated by gas cluster ion beam(GCIB)or by accelerated neutral atom beam(ANAB)result in a nanoscale-modified surface topography that changes the ability of extracellular proteins to bind.This leads to decreased bacterial attachment and an attenuated inflammatory response using both in vitro and in vivo assays.Differential adsorption of extracellular proteins to the polymeric surface improved the competitive attachment of osteoblasts over bacteria,without resorting to growth factor of antibiotic use. | Joseph Khoury Ti Zhang David B.Earle M.Laird Forrest | 2023 | Engineered Regeneration2023，4，3： | 0 |
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