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2篇 您的检索式：作者名="David Ando"

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		题名	作者	年代	出处	被引量
	1	Cartilage repair using an in vitro generated scaffold-free tissue-engineered construct derived from porcine synovial mesenchymal stem cells显示文摘	Wataru Ando Kosuke Tateishi David A. Hart Daisuke Katakai Yoshinari Tanaka Ken Nakata Jun Hashimoto Hiromichi Fujie Konsei Shino Hideki Yoshikawa Norimasa Nakamura	2007	Biomaterials2007，，36：	1
	2	Scalable and automated CRISPR-based strain engineering using droplet microfluidics显示文摘We present a droplet-based microfluidic system that enables CRISPR-based gene editing and high-throughput screening on a chip.The microfluidic device contains a 10x10 element array,and each element contains sets of electrodes for two electric field-actuated operations:electrowetting for merging droplets to mix reagents and electroporation for transformation.This device can perform up to 100 genetic modification reactions in parallel,providing a scalable platform for generating the large number of engineered strains required for the combinatorial optimization of genetic pathways and predictable bioengineering.We demonstrate the system's capabilities through the CRISPR-based engineering of two test cases:(1)disruption of the function of the enzyme galactokinase(galK)in£coli and(2)targeted engineering of the glutamine synthetase gene(glnA)and the blue-pigment synthetase gene(bpsA)to improve indigoidine production in£coli.	Kosuke Iwai Maren Wehrs Megan Garber Jess Sustarich Lauren Washburn Zachary Costello Peter W.Kim David Ando William R.Gaillard Nathan J.Hillson Paul D.Adams Aindrila Mukhopadhyay Hector Garcia Martin Anup K.Singh	2022	Microsystems & Nanoengineering2022，8，2：	0

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