| 1 | 樟疫多聚半乳糖醛酸酶Pcpg1、Pcpg2和Pcpg4基因的克隆、测序及其遗传转化显示文摘研究优化了樟疫(Phytophthoracinnamomi)多聚半乳糖醛酸酶Pcpg1、Pcpg2和Pcpg4基因克隆的PCR条件,并对其进行了克隆、测序和遗传转化。克隆获得了3个基因,其大小均为1011bp;通过构建表达载体和遗传转化获得了3个基因的转基因菌系;3个基因均能指导合成相应的PG酶,其中转化Pcpg2基因的酵母菌(Saccharomycescerevisiae)所分泌的多聚半乳糖醛酸酶(PG)酶活性最强,Pcpg4和对照Anpg(Aspergillusnigerpolygalacturonase)I次之,而Pcpg1基因所指导合成的PG酶无活性。Westernblotting表明,所克隆的3个基因所指导合成的PG酶均有不同程度的糖基化。 | 巩振辉 Arvid Gotesson David A.Jones | 2003 | 农业生物技术学报2003，11，5： | 5 |
| 5 | Bipolar Disorder in Geopolitical Strategy Within Eurasia: When Will the “New Great Game” Adjust to Reality?显示文摘Current geopolitical strategies of countries or trading partners reflect a“New Great Game”in the wake of the Covid19 pandemic,especially across Eurasia.Strategies range from modification of China’s decade-long“Belt and Road”Initiative,Russian Federation’s Eurasian Union,to Japan’s Free and Open Indo-Pacific geopolitical program,coupled with“Build Back Better World”(“B3W”)initiative advanced by the Group of Seven(G7)at its 12 June 2021 meeting at Carbis Bay,Cornwall,England.This came as United States forces withdrew from Afghanistan chaotically.Russia,China,and the West each compete to become the more effective chess player in Eurasia.Competitor countries have developed traditional and novel soft power tools to construct relationships across that supercontinent.What countries will endure,meet the test of time?This chapter will address those issues individually,collectively,drawing upon forecasts advanced in this century,post-pandemic assessments,with triangulation of information available currently in the literature of“New Great Game”players.Much is at stake,from Eurasia’s future to prospective statehood in East Turkmenistan(China’s Xinjiang)to peace across Asia,global tranquility.All nations must meet global expectations and regulations,be rewarded when they do,ostracized in failure.Brazil,India,and countries driving trade should be considered seriously for greater roles in global governance. | David A.Jones | 2022 | International Relations and Diplomacy2022，10，5： | 0 |
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