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| 1 | Cell sheet technology for regeneration of esophageal mucosa显示文摘The progress of tissue-engineering technology has realized development of new therapies to treat various disorders by using cultured cells. Cell-and tissue-based therapies have been successfully applied to human patients, and several tissue-engineered products have been approved by the regulatory agencies and are commercially available. In the review article, we describe our experience of development and clinical application of cell sheet-based regenerative medicine.Endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) have been shown to be useful for removal of gastrointestinal neoplasms with less invasiveness compared with open surgery, especially in esophageal surgery. However, postoperative inflammation and stenosis are major complications observed after intensive mucosal resection. Therefore, we have developed novel regenerative medicine to prevent such complications and promote wound healing of esophageal mucosa after EMR or ESD. Transplantable oral mucosal epithelial cell sheets were fabricated from patients' own oral mucosa. Immediately after EMR or ESD, fabricated autologous cell sheets were endoscopically transplanted to the ulcer sites. We performed a preclinical study with a canine model. In human clinical settings, cell culture and cell sheet fabrication were performed in clean rooms according to good manufacturing practice guidelines, and pharmaceutical drugs were used as supplements to culture medium in place of research regents used in animal study. We believe that cell-based regenerative medicine would be useful to improve quality of life of patients after EMR or ESD. | Ryo Takagi Masayuki Yamato Nobuo Kanai Daisuke Murakami Makoto Kondo Takaaki Ishii Takeshi Ohki Hideo Namiki Masakazu Yamamoto Teruo Okano | 2012 | World Journal of Gastroenterology2012，18，37： | 9 |
| 2 | Hepatocellular carcinoma in extremely elderly patients:An analysis of clinical characteristics,prognosis and patient survival显示文摘瞄准:80 年或更多与肝细胞癌(HCC ) 识别病人的临床、预示的特征。方法:有 HCC 的 1310 个病人的一个总数在这研究被包括。80 年或更多的 91 个病人在 HCC 的诊断的时候被定义为极其老的组。>
或 = 的 234 个病人 50 年但是不到 60 年被认为是非老的组。结果:两性比率(到女性的男性) 比在非老的组在极其老的组(0.90:1 ) 是显著地更低的(3.9:1, P <
0.001 ) 。为 HBsAg 的积极的率在极其老的组和为显然在极其老的组增加的 HBsAg 和 HCVAb 否定的病人的比例是显著地更低的(P <
0.001 ) 。在下列参数没有有效差量:肿瘤,分级的孩子呸,肿瘤阶段,门静脉血栓形成或腹水的存在,和为 HCVAb 的积极的率的直径和数字。极其老的病人经常没收到外科疗法(P <
0.001 ) 并且他们是更可能的收到保守疗法(P <
0.01 ) 。基于与在二个组之间的全面病人比较的 Kaplan-Meier 方法在幸存曲线没有有效差量。然而,幸存曲线在有阶段 I/II 的极其老的病人是显著地更坏的,上演 I/II,孩子呸分级与非老的组比较的肝硬化。死亡的原因没在病人之中不同,并且大多数盒子甚至在极其老的病人死于肝相关的疾病。结论:在有好肝功能和好表演地位的病人,为 HCC 的好攻击的治疗可能改进幸存率,甚至在极其老的病人。 | Gengo Tsukioka Satoru Kakizaki Naondo Sohara Ken Sato Hitoshi Takagi Hirotaka Arai Takehiko Abe Mitsuo Toyoda Kenji Katakai Akira Kojima Yuichi Yamazaki Toshiyuki Otsuka Yutaka Matsuzaki Fujio Makita Daisuke Kanda Katsuhiko Horiuchi Tetsuya Hamada Mieko Kaneko Hideyuki Suzuki Masatomo Mori | 2006 | World Journal of Gastroenterology2006，12，1： | 7 |
| 3 | Single-Walled Carbon Nanotube Growth with Non-Iron- Group “Catalysts” by Chemical Vapor Deposition显示文摘Various materials have been found to“catalyze”carbon nanotube growth in chemical vapor deposition(CVD)when they become nano-sized particles.These involve not only metals,such as Pd,Pt,Au,Ag,and Cu,but also semiconductors,such as Si,Ge,and SiC.Alumina and diamond nanoparticles also produce carbon nanotubes.These“catalysts”,which are better called“seeds”,can be categorized into two types:one type forms a eutectic liquid or highly-mobile alloy with carbon,and carbon atoms precipitate from the eutectic alloy;the other type remains as a solid phase and form a carbon surface layer during CVD growth.In this paper,we review recent studies of SWCNT growth with these non-iron-group materials and highlight the mechanisms involved. | Yoshikazu Homma Huaping Liu Daisuke Takagi Yoshihiro Kobayashi | 2009 | Nano Research2009，2，10： | 6 |
| 4 | Gastrointestinal stromal tumor presenting with prominent calcification显示文摘We present a rare case of a gastrointestinal stromal tumor (GIST) in the stomach with prominent calcifica-tion at presentation. A 61-year-old woman visited our hospital because of epigastric discomfort. A spherical calcified lesion with a diameter of about 30 mm was incidentally shown in the left upper quadrant on an abdominal X-ray. Computed tomography demonstrated that the tumor was growing from the upper gastric body, with calcification in the peripheral ring area. A laparoscopic partial gastrectomy was performed, and the resected specimen revealed a well-circumscribed tumor with exophytic growth from the gastric muscularis propria. Microscopic examination revealed spindle- shaped tumor cells with calcification and hemorrhage. Additionally, positive immunoreactivity of the tumor to KIT and CD34 and a low mitotic index resulted in the diagnosis of very low risk GIST. There are a few case reports of heavily calcified GIST, although solitary or punctate calcification of primary GIST has been reported in several case series. Dystrophic calcification of necrotic or degenerative tissue is the supposed cause of primary calcified GISTs. In contrast, appearance of calcification after administration of imatinib mesylate, which may be one indicator of disease response, is possibly caused by a different mechanism. | Naoki Izawa Takeshi Sawada Ryuichi Abiko Daisuke Kumon Mami Hirakawa Mika Kobayashi Nobuyuki Obinata Masahito Nomoto Tadateru Maehata Shun-ichi Yamauchi Takefumi Kouro Takashi Tsuda Satoshi Kitajima Hiroshi Yasuda Keiichi Tanaka Ichiro Tanaka Masahiro Hoshikawa Masayuki Takagi Fumio Itoh | 2012 | World Journal of Gastroenterology2012，18，39： | 2 |
| 5 | Overexpression of NK2 inhibits liver regeneration after partial hepatectomy in mice显示文摘AIM: To investigate the in vivo effects of NK2 on liver regeneration after partial hepatectomy (PH). METHODS: Survival after PH was observed with 21 NK2 transgenic mice and 23 wild-type (WT) mice over 10 d. Liver regeneration was analyzed using histology and immunohistochemistry. Expressions of genes were analyzed using Northern blot analysis, immunoprecipitation and immunoblotting, and reverse transcriptase polymerase chain reaction assay. KaplanMeier method and the log-rank test were used for ahalyzing the survival after PH. Differences in the resultsof immunohistochemistry and percentage of liver regeneration was determined by the Student's t-test. RESULTS: More than half of NK2 transgenic mice died within 48 h after PH. After PH, increased deposition of small lipid droplets in hepatocytes was evident and hepatic proliferation was inhibited in NK2 transgenic mice. The hepatic expression and kinase activity of HGF receptor, c-Met, were unchanged among WT mice and NK2 transgenic mice after PH. The expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in liver tissues were prolonged in NK2 transgenic mice that died after PH.CONCLUSION: Our findings indicate that overexpression of NK2 inhibits liver regeneration after PH. | Toshiyuki Otsuka Norio Horiguchi Daisuke Kanda Takashi Kosone Yuichi Yamazaki Kazuhisa Yuasa Naondo Sohara Satoru Kakizaki Ken Sato Hitoshi Takagi Glenn Merlino Masatomo Mori | 2005 | World Journal of Gastroenterology2005，11，47： | 2 |
| 6 | Vitreous levels of angiopoietin 2 and vascular endothelial growth factor in patients with proliferative diabetic retinopathy显示文摘 | Daisuke Watanabe Kiyoshi Suzuma Izumi Suzuma Hirokazu Ohashi Tomonari Ojima Masafumi Kurimoto Tomoaki Murakami Tetsushi Kimura Hitoshi Takagi | 2005 | American Journal of Ophthalmology2005，，3： | 1 |
| 7 | Vitreous levels of angiopoietin 2 and vascular endothelial growth factor in patients with proliferative diabetic retinopathy显示文摘 | Daisuke Watanabe Kiyoshi Suzuma Izumi Suzuma Hirokazu Ohashi Tomonari Ojima Masafumi Kurimoto Tomoaki Murakami Tetsushi Kimura Hitoshi Takagi | 2005 | American Journal of Ophthalmology2005，，3： | 1 |
| 8 | Novel role of erythropoietin in proliferative diabetic retinopathy显示文摘 | Hitoshi Takagi Daisuke Watanabe Kiyoshi Suzuma Masafumi Kurimoto Izumi Suzuma Hirokazu Ohashi Tomonari Ojima Tomoaki Murakami | 2007 | Diabetes Research and Clinical Practice2007，，3： | 1 |
| 9 | Experimental study of a low-alkali tholeiite at 1–5?kbar: optimal condition for the crystallization of high-An plagioclase in hydrous arc tholeiite显示文摘 | Daisuke Takagi Hiroaki Sato Mitsuhiro Nakagawa | 2005 | Contributions to Mineralogy and Petrology2005，，5： | 1 |
| 10 | High-precision three-dimensional inkjet technology for live cell bioprinting显示文摘In recent years,bioprinting has emerged as a promising technology for the construction of three-dimensional(3D)tissues to be used in regenerative medicine or in vitro screening applications.In the present study,we present the development of an inkjet-based bioprinting system to arrange multiple cells and materials precisely into structurally organized constructs.A novel inkjet printhead has been specially designed for live cell ejection.Droplet formation is powered by piezoelectric membrane vibrations coupled with mixing movements to prevent cell sedimentation at the nozzle.Stable drop-on-demand dispensing and cell viability were validated over an adequately long time to allow the fabrication of 3D tissues.Reliable control of cell number and spatial positioning was demonstrated using two separate suspensions with different cell types printed sequentially.Finally,a process for constructing stratified Mille-Feuille-like 3D structures is proposed by alternately superimposing cell suspensions and hydrogel layers with a controlled vertical resolution.The results show that inkjet technology is effective for both two-dimensional patterning and 3D multilayering and has the potential to facilitate the achievement of live cell bioprinting with an unprecedented level of precision. | Daisuke Takagi Waka Lin Takahiko Matsumoto Hidekazu Yaginuma Natsuko Hemmi Shigeo Hatada Manabu Seo | 2019 | International Journal of Bioprinting2019，5，2： | 1 |
| 11 | An Ultra-Low On-Resistan ce Power MOSFET Fabricated by Using a Fully Self-Aligned Process显示文摘 | Daisuke Ueda Hiromitsu Takagi and Gota Kano | 1987 | IEEE Tr ans On ED1987，34，4： | 1 |
| 12 | Stent length is impact factor associated with stent patency in endoscopic ultrasound‐guided hepaticogastrostomy显示文摘 | Takeshi Ogura Kazuhiro Yamamoto Tatsushi Sano Saori Onda Akira Imoto Daisuke Masuda Wataru Takagi Shinya Fukunishi Kazuhide Higuchi | 2015 | Journal of Gastroenterology and Hepatology2015，，12： | 1 |
| 13 | EphrinA1 Inhibits Vascular Endothelial Growth Factor-Induced Intracellular Signaling and Suppresses Retinal Neovascularization and Blood-Retinal Barrier Breakdown显示文摘 | Tomonari Ojima Hitoshi Takagi Kiyoshi Suzuma Hideyasu Oh Izumi Suzuma Hirokazu Ohashi Daisuke Watanabe Eri Suganami Tomoaki Murakami Masafumi Kurimoto Yoshihito Honda Nagahisa Yoshimura | 2006 | The American Journal of Pathology2006，，1： | 1 |
| 14 | A novel difficulty scoring system for laparoscopic liver resection显示文摘 | Daisuke Ban Minoru Tanabe Hiromitsu Ito Yuichiro Otsuka Hiroyuki Nitta Yuta Abe Yasushi Hasegawa Toshio Katagiri Chisato Takagi Osamu Itano Hironori Kaneko Go Wakabayashi | 2014 | J Hepatobiliary Pancreat Sci2014，，10： | 1 |
| 15 | Prevention of Esophageal Stricture After Endoscopic Submucosal Dissection Using Tissue-Engineered Cell Sheets显示文摘 | Takeshi Ohki Masayuki Yamato Masaho Ota Ryo Takagi Daisuke Murakami Makoto Kondo Ryo Sasaki Hideo Namiki Teruo Okano Masakazu Yamamoto | 2012 | Gastroenterology2012，，3： | 1 |
| 16 | LOH analysis of free DNA in the plasma of patients with mucosal malignant melanoma in the head and neck显示文摘 | Ryo Takagi Daisuke Nakamoto Jun-etsu Mizoe Hirohiko Tsujii | 2007 | International Journal of Clinical Oncology2007，，3： | 1 |
| 17 | Pancreatic and gastric metastases of leiomyosarcoma arising in the left leg显示文摘 | Yoshimi Nakajima Satoru Kakizaki Daisuke Kanda Yasushi Shimada Naondo Sohara Ken Sato Hitoshi Takagi Masatomo Mori Hideomi Watanabe | 2005 | International Journal of Clinical Oncology2005，，5： | 1 |
| 18 | Vitreous levels of angiopoietin 2 and vascular endothelial growth factor in patients with proliferative diabetic retinopathy显示文摘 | Daisuke Watanabe Kiyoshi Suzuma Izumi Suzuma Hirokazu Ohashi Tomonari Ojima Masafumi Kurimoto Tomoaki Murakami Tetsushi Kimura Hitoshi Takagi | 2005 | American Journal of Ophthalmology2005，，3： | 1 |
| 19 | Association between ADAMTS13 activity-VWF antigen imbalance and the therapeutic effect of HAIC in patients with hepatocellular carcinoma显示文摘BACKGROUND Prediction of HAIC treatment response is important for improving the prognosis in patients with hepatocellular carcinoma(HCC).The progression of HCC is related to hypercoagulability and angiogenesis.It is known that ADAMTS13 and von Willebrand factor(VWF)are related to hypercoagulability.In addition,previous study reported that the association between ADAMTS13 and VWF,and angiogenesis via vascular endothelial growth factor(VEGF).Recently,ADAMTS13 and VWF have been associated with the prognosis in patients with various kinds of cancer undergoing chemotherapy.AIM To investigate whether ADAMTS13 and VWF become useful biomarkers of treatment response in HCC patients before the initiation of HAIC treatment.METHODS Seventy-two patients were enrolled in this study.ADAMTS13 activity(ADAMTS13:AC),VWF antigen(VWF:Ag)and VEGF levels were determined via enzyme-linked immunosorbent assay.Univariable and multivariable analyses were performed to determine the predictive factors of treatment response in patients with HCC undergoing HAIC treatment.RESULTS ADAMTS13:AC levels in HCC patients with stable disease(SD)+partial response(PR)of HAIC treatment were significantly higher than those with progressive disease(PD)(P<0.05).In contrast,VWF:Ag/ADAMTS13:AC ratio and VEGF levels in HCC patients with SD+PR were significantly lower than those with PD(both P<0.05).Patients with high VWF:Ag/ADAMTS13:AC ratio(>2.7)had higher VEGF levels than those with low ratio(≤2.7).Multivariable analysis revealed that VWF:Ag/ADAMTS13:AC ratio was a predictive factor of HAIC treatment response.CONCLUSION VWF:Ag/ADAMTS13:AC ratio may become a useful biomarker of treatment response in HCC patients before the initiation of HAIC treatment. | Hiroaki Takaya Tadashi Namisaki Kei Moriya Naotaka Shimozato Kosuke Kaji Hiroyuki Ogawa Koji Ishida Yuki Tsuji Daisuke Kaya Hirotestu Takagi Yukihisa Fujinaga Norihisa Nishimura Yasuhiko Sawada Hideto Kawaratani Takemi Akahane Masanori Matsumoto Hitoshi Yoshiji | 2020 | World Journal of Gastroenterology2020，26，45： | 0 |
| 20 | Triple therapy of interferon and ribavirin with zinc supplementation for patients with chronic hepatitis C:A randomized controlled clinical trial显示文摘瞄准:为了与锌学习干扰素(IFN ) 和 ribavirin 的治疗学的效果,与长期的丙肝在病人上补充病毒(HCV ) 感染。方法:组织学地证实与遗传型 1b 和 HCV 的超过 100 KIU/mL 有长期的 HCV 感染的 102 个病人的一个总数随机被分到学习的每只手臂,各个收到了为 4 wk 的干扰素( IFN-alpha-2b )日报每隔一天由一样的剂量跟随了为的 pegylated 的1000万个单位加 ribavirin ( 600 或 800 mg/d 取决于体重)的 20 wk ,与或没有 polaprezinc ( 150 mg/d )口头上地为 24 wk 。主要端点是在浆液定义为否定 HCV-RNA 的持续 virological 反应(SVR ) 6 瞬间术后疗法。结果:比在另外的组(P<0.05 ) 除了在双治疗组的更多的女性在在二个组之间的临床的背景没有差别。SVR 在 33.3% 三倍的治疗组和 33.3% 双治疗组被观察。副作用是几乎,在两个的一样除了胃肠的症状组织,它是在三倍的治疗组(P=0.019 ) 的更少。结论:一起考虑了,加为有遗传型 1b 和高病毒的负担的 HCV 感染病人的 IFN 和 ribavirin 的锌的三倍的治疗不除了胃肠的副作用的更低的发生比双治疗好。 | Hideyuki Suzuki Hitoshi Takagi Naondo Sohara Daisuke Kanda Satoru Kakizaki Ken Sato Masatomo Mori | 2006 | World Journal of Gastroenterology2006，12，8： | 0 |
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