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| 1 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 2 | T cell-depleting nanoparticles ameliorate bone loss by reducing activated T cells and regulating the Treg/Th17 balance显示文摘Estrogen deficiency is one of the most frequent causes of osteoporosis in postmenopausal women.Under chronic inflammatory conditions caused by estrogen deficiency,activated T cells contribute to elevated levels of proinflammatory cytokines,impaired osteogenic differentiation capabilities of bone marrow mesenchymal stem cells(BMMSCs),and disturbed regulatory T cell(Treg)/Th17 cell balance.However,therapeutic strategies that re-establish immune homeostasis in this disorder have not been well developed.Here,we produced T cell-depleting nanoparticles(TDNs)that ameliorated the osteopenia phenotype and rescued the osteogenic deficiency of BMMSCs in ovariectomized(OVX)mice.TDNs consist of monocyte chemotactic protein-1(MCP-1)-encapsulated mesoporous silica nanoparticles as the core and Fas-ligand(FasL)as the corona.We showed that the delicate design of the TDNs enables rapid release of MCP-1 to recruit activated T cells and then induces their apoptosis through the conjugated FasL both in vitro and in vivo.Apoptotic signals recognized by macrophages help skew the Treg/Th17 cell balance and create an immune tolerant state,further attenuating the osteogenic deficiency of BMMSCs and the osteopenia phenotype.Mechanistically,we found that the therapeutic effects of TDNs were partially mediated by apoptotic T cell-derived extracellular vesicles(ApoEVs),which promoted macrophage transformation towards the M2 phenotype.These findings demonstrate that TDNs may represent a promising strategy for treating osteoporosis and other immune disorders. | Xiaoshan Yang Fuxing Zhou Pingyun Yuan Geng Dou Xuemei Liu Siying Liu Xiangdong Wang Ronghua Jin Yan Dong Jun Zhou Yajie Lv Zhihong Deng Shiyu Liu Xin Chen Ying Han Yan Jin | 2021 | Bioactive Materials2021，6，10： | 4 |
| 3 | Effect of Carbonation and Drying-Wetting Cycles on Chloride Diffusion Behavior of Coral Aggregate Seawater Concrete显示文摘Based on seawater immersion,drying-wetting cycles,carbonation and drying-wetting cycles for coral aggregate sea-water concrete(CASC)with different strength grades,the effect of carbonation and drying-wetting cycles on chloride diffusion be-havior of CASC is studied.The results show that the free surface chloride concentration(Cs),free chloride diffusion coefficient(Df)and time-dependent index(m)of CASC in the drying-wetting cycles is obviously higher than that in seawater immersion.The Df and m of CASC of carbonation and drying-wetting cycles is higher than that in the drying-wetting cycles.Carbonation increases the Df and m of CASC,which is against CASC to resist chloride corrosion.The corrosion possibility of CASC structures in different ex-posed areas is as follows:splash zone(carbonation and drying-wetting cycles)>tidal zone(drying-wetting cycles)>underwater zone(seawater immersion).Besides,the chloride diffusion rate of C65-CASC is 17.8%-63.4%higher than that of C65-ordinary aggre-gate concrete(OAC)in seawater immersion(underwater zone).Therefore,anti-corrosion measures should be adopted to improve the service life of CASC structure in the oceanic environment. | DA Bo LI Yipeng YU Hongfa MA Haiyan CHEN Haoyu DOU Xuemei WU Zhangyu | 2022 | Journal of Ocean University of China2022，21，1： | 2 |
| 4 | Metabolomics study on the anti-depression effect of xiaoyaosan on rat model of chronic unpredictable mild stress显示文摘 | Yuntao Dai Zhenyu Li Liming Xue Chunyan Dou Yuzhi Zhou Lizeng Zhang Xuemei Qin | 2010 | Journal of Ethnopharmacology2010，，2： | 1 |
| 5 | A Global Analysis of the Relationship Between Urbanization and Fatalities in Earthquake-Prone Areas显示文摘Urbanization can be a challenge and an opportunity for earthquake risk mitigation.However,little is known about the changes in exposure(for example,population and urban land)to earthquakes in the context of global urbanization,and their impacts on fatalities in earthquake-prone areas.We present a global analysis of the changes in population size and urban land area in earthquake-prone areas from 1990 to 2015,and their impacts on earthquake-related fatalities.We found that more than two thirds of population growth(or 70%of total population in 2015)and nearly three quarters of earthquake-related deaths(or 307,918 deaths)in global earthquake-prone areas occurred in developing countries with an urbanization ratio(percentage of urban population to total population)between 20 and 60%.Holding other factors constant,population size was significantly and positively associated with earthquake fatalities,while the area of urban land was negatively related.The results suggest that fatalities increase for areas where the urbanization ratio is low,but after a ratio between 40 and 50%occurs,earthquake fatalities decline.This finding suggests that the resistance of building and infrastructure is greater in countries with higher urbanization ratios and highlights the need for further investigation.Our quantitative analysis is extended into the future using Shared Socioeconomic Pathways to reveal that by 2050,more than 50%of the population increase in global earthquake-prone areas will take place in a few developing countries(Pakistan,India,Afghanistan,and Bangladesh)that are particularly vulnerable to earthquakes.To reduce earthquake-induced fatalities,enhanced resilience of buildings and urban infrastructure generally in these few countries should be a priority. | Chunyang He Qingxu Huang Xuemei Bai Derek TRobinson Peijun Shi Yinyin Dou Bo Zhao Jubo Yan Qiang Zhang Fangjin Xu James Daniell | 2021 | International Journal of Disaster Risk Science2021，12，6： | 1 |
| 6 | Preparation of Functionalized Fe304@SiO2 MagneticNanoparticles for Monoclonal Antibody Purification显示文摘Magnetic Fe304@SiO2 nanoparticles with superparamagnetic properties were prepared via a reverse mi-croemulsion method at room temperature. The as-prepared samples were characterized by transmission electron mi-croscopy(TEM), X-ray diffractometry(XRD), and vibrating sample magnetometry(VSM). The Fe304@SiO2 nanopar-ticles were modified by (3-aminopropyl)triethoxysilane(APTES) and subsequently activated by glutaraldehyde(Glu).Protein A was successfully immobilized covalently onto the Glu activated Fe304@SiO2 nanoparticles. The adsorptioncapacity of the nanoparticles was determined on an ultraviolet spectrophotometer(UV) and approximately up to 203mg/g of protein A could be uniformly immobilized onto the modified Fe304@SiO2 magnetic beads. The core-shell ofthe Fe304@SiO2 magnetic beads decorated with protein A showed a good binding capacity for the chime-ric anti-EGFR monoclonal antibody(anti-EGFR mAb). The purity of the anti-EGFR mAb was analyzed by virtue ofHPLC. The protein A immobilized affinity beads provided a purity of about 95.4%. | HOU Xuemei ZHAO Changjie TIAN Yanlong DOU Shuliang ZHANG Xiang ZHAO Jiupeng | 2016 | Chemical Research in Chinese Universities2016，32，6： | 0 |
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