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| 1 | Clinical utility of Epstein-Barr virus DNA and other liquid biopsy markers in nasopharyngeal carcinoma显示文摘Nasopharyngeal carcinoma(NPC)is a malignant epithelial tumor ubiquitously associated with the Epstein-Barr virus(EBV),which is highly prevalent in South China,Southeast Asia,and North Africa.Despite being a highly radio-sensitive and treatable cancer,a majority of NPC patients are diagnosed in their advanced stage,and locoregional and distant relapses following definitive treatment contribute largely to cancer-specific mortality among these patients.Given that EBV-driven NPC is the predominant variant seen in endemic regions,various EBV detection methods have been developed and are utilized in screening,prognostication,and post-treatment surveillance of NPC patients.While the Immunoglobulin A(IgA)serology assay is the most extensively studied EBV detection method,the detection of plasma EBV DNA released during replication or cellular apoptosis has shown superior outcomes in endemic population screening,prognostication,and detection of distant relapse.Furthermore,there is emerging evidence on the use of circulating tumor cells,microRNAs,DNA hypermethylation,and combination assays in various clinical scenarios.Herein,this paper provides a comprehensive overview of the relevant studies using various EBV detection techniques in the management of NPC.Specifically,the recent advances,clinical evidence,and challenges associated with the clinical application of EBV liquid biopsies in population screening,prognostication,and surveillance of NPC are presented. | Rong Tan Sean Kean Ann Phua Yoke Lim Soong Lynette Lin Ean Oon Kian Sing Chan Sasidharan Swarnalatha Lucky Jamie Mong Min Han Tan Chwee Ming Lim | 2020 | Cancer Communications2020，40，11： | 8 |
| 2 | Functional micro‐concrete 3D hybrid structures f abricated by two‐photon polymerization显示文摘Arbitrary micro-scale three-dimensional(3D)structures fabrication is a dream to achieve many exciting goals that have been pursued for a long time.Among all these applications,the direct 3D printing to fabricate human organs and integrated photonic circuits are extraordinary attractive as they can promote the current technology to a new level.Among all the 3D printing methods available,two-photon polymerization(2PP)is very competitive as it is the unique method to achieve sub-micron resolution to make any desired tiny structures.For the conventional 2PP,the building block is the photoresist.However,the requirement for the building block is different for different purposes.It is very necessary to investigate and improve the photoresist properties according to different requirements.In this paper,we presented one hybrid method to modify the mechanical strength and light trapping efficiency of the photoresist,which transfers the photoresist into the micro-concretes.The micro-concrete structure can achieve±22%strength modification via a silica nano-particles doping.The structures doped with gold nano-particles show tunable plasmonic absorption.Dye doped hybrid structure shows great potential to fabricate 3D micro-chip laser. | Yang Li Lianwei Chen Fang Kong Zuyong Wang Ming Dao Chwee Teck Lim Fengping Li Minghui Hong | 2017 | 光电工程2017，44，4： | 2 |
| 3 | Retreatment in locally recurrent nasopharyngeal carcinoma:Current status and perspectives显示文摘1 Background Nasopharyngeal carcinoma(NPC)arises from the epithelial cells that cover and line the nasopharynx.While it is considered a rare cancer globally,it is commonly observed in South China and a few other ethnically distinct racial groups.Due to its propensity to spread early through the submucosal tissue and the highly infiltrative nature of this disease,NPC spreads easily through areas of lesser resistance within the pharyngobasilar fascia with a tendency for neural infiltration[1-3]. | Sharon Shuxian Poh Yoke Lim Soong Kiattisa Sommat Chwee Ming Lim Kam Weng Fong Terence WK Tan Melvin LK Chua Fu Qiang Wang Jing Hu Joseph TS Wee | 2021 | Cancer Communications2021，41，5： | 1 |
| 4 | Pan-CDK inhibition augments cisplatin lethality in nasopharyngeal carcinoma cell lines and xenograft models显示文摘In addition to their canonical roles in regulating cell cycle transition and transcription,cyclin-dependent kinases(CDKs)have been shown to coordinate DNA damage response pathways,suggesting a rational pairing of CDK inhibitors with genotoxic chemotherapeutic agents in the treatment of human malignancies.Here,we report that roniciclib(BAY1000394),a potent pan-CDK inhibitor,displays promising anti-neoplastic activity as a single agent and potentiates cisplatin lethality in preclinical nasopharyngeal carcinoma(NPC)models.Proliferation of the NPC cell lines HONE-1,CNE-2,C666-1,and HK-1 was effectively curbed by roniciclib treatment,with IC_(50)values between 11 and 38 nmol/L.These anticancer effects were mediated by pleiotropic mechanisms consistent with successful blockade of cell cycle CDKs 1,2,3,and 4 and transcriptional CDKs 7 and 9,ultimately resulting in arrest at G1/S and G2/M,downregulation of the transcriptional apparatus,and repression of anti-apoptotic proteins.Considerably enhanced tumor cell apoptosis was achieved following combined treatment with 10 nmol/L roniciclib and 2.0μmol/L cisplatin;this combination therapy achieved a response over 250%greater than either drug alone.Although roniciclib chemosensitized NPC cells to cisplatin,it did not sensitize untransformed(NP69)cells.The administration of 0.5 mg/kg roniciclib to BALB/c xenograft mice was well tolerated and effectively restrained tumor growth comparable to treatment with 6 mg/kg cisplatin,whereas combining these two agents produced far greater tumor suppression than either of the monotherapies.In summary,these data demonstrate that roniciclib has strong anti-NPC activity and synergizes with cisplatin chemotherapy at clinically relevant doses,thus justifying further evaluation of this combinatorial approach in clinical settings. | Nicholas LSyn Pei Li Lim Li Ren Kong Lingzhi Wang Andrea Li-Ann Wong Chwee Ming Lim Thomas Kwok Seng Loh Gerhard Siemeister Boon Cher Goh Wen-Son Hsieh | 2018 | Signal Transduction and Targeted Therapy2018，3，1： | 0 |
| 5 | Modular micro-PCR system for the onsite rapid diagnosis of COVID-19显示文摘Effective containment of the COVID-19 pandemic requires rapid and accurate detection of the pathogen.Polymerase chain reaction(PCR)remains the gold standard for COVID-19 confirmation.In this article,we report the performance of a cost-effective modular microfluidic reverse transcription(RT)-PCR and RT-loop mediated isothermal amplification(RTLAMP)platform,Epidax®,for the point-of-care testing and confirmation of SARS-CoV-2.This platform is versatile and can be reconfigured either for screening using endpoint RT-PCR or RT-LAMP tests or for confirmatory tests using realtime RT-PCR.Epidax®is highly sensitive and detects as little as 1 RNA copy perµL for real-time and endpoint RT-PCR,while using only half of the reagents.We achieved comparable results with those of a commercial platform when detecting SARS-CoV-2 viruses from 81 clinical RNA extracts.Epidax®can also detect SARS-CoV-2 from 44 nasopharyngeal samples without RNA extraction by using a direct RT-PCR assay,which shortens the sample-to-answer time to an hour with minimal user steps.Furthermore,we validated the technology using an RT-LAMP assay on 54 clinical RNA extracts.Overall,our platform provides a sensitive,cost-effective,and accurate diagnostic solution for lowresource settings. | Phuong Quoc Mai Nguyen Ming Wang Nelisha Ann Maria Adelicia Yongling Li Hsih Yin Tan Gordon Minru Xiong Meng-Kwang Marcus Tan Ali Asgar S.Bhagat Catherine W.M.Ong Chwee Teck Lim | 2022 | Microsystems & Nanoengineering2022，8，4： | 0 |
| 6 | Real time in-vivo miniature endoscopic surveillance system for imaging of nasopharynx显示文摘Objective:To test the feasibility of a real time miniature endoscope system for imaging the nasopharynx.Study design:Preclinical assessment on skull model and cadaver.Methods:A 3.5 mm miniature endoscope was fabricated and the image capture of the nasopharynx was investigated by positioning the miniature camera system at the posterior free edge of the vomer bone.Wireless real time transmission of the images and quality was tested in a skull model.Next,three nasopharyngeal surveillance miniature camera system were developed for possible clinical translation.Two prototypes were anchored on the nasal septum and the last prototype was designed using a patient self-administered surveillance process.These prototypes were tested for feasibility on both the phantom skull and cadaveric model.Risk assessments were also performed to assess risk,safety and validate the reliability of the material utilized for clinical translation.Results:Insertion and anchorage of the miniature surveillance endoscope prototypes at the vomer bone were feasible on all 3 prototypes.The quality of captured images was reasonable and miniaturized camera was responsive to pan at different angles so that the entire nasopharynx may be surveyed.Risk assessments on the material such as pull out test,breaking force analysis,finite element test and tensile strength test were reliable for possible clinical translation.Conclusions:Real time miniature endoscope system for surveillance of nasopharyngeal cancer is feasible.Clinical translation of this technology was possible but requires further refinement in enhancing image quality and wireless transmission of the captured images. | Chwee Ming Lim Gangodu Nagaraja Rao Hongliang Ren | 2020 | World Journal of Otorhinolaryngology-Head and Neck Surgery2020，6，1： | 0 |
| 7 | Mechano-responsive hydrogel for direct stem cell manufacturing to therapy显示文摘Bone marrow-derived mesenchymal stem cell(MSC)is one of the most actively studied cell types due to its regenerative potential and immunomodulatory properties.Conventional cell expansion methods using 2D tissue culture plates and 2.5D microcarriers in bioreactors can generate large cell numbers,but they compromise stem cell potency and lack mechanical preconditioning to prepare MSC for physiological loading expected in vivo.To overcome these challenges,in this work,we describe a 3D dynamic hydrogel using magneto-stimulation for direct MSC manufacturing to therapy.With our technology,we found that dynamic mechanical stimulation(DMS)enhanced matrix-integrinβ1 interactions which induced MSCs spreading and proliferation.In addition,DMS could modulate MSC biofunctions including directing MSC differentiation into specific lineages and boosting paracrine activities(e.g.,growth factor secretion)through YAP nuclear localization and FAK-ERK pathway.With our magnetic hydrogel,complex procedures from MSC manufacturing to final clinical use,can be integrated into one single platform,and we believe this‘all-in-one’technology could offer a paradigm shift to existing standards in MSC therapy. | Yufeng Shou Ling Liu Qimin Liu Zhicheng Le Khang Leng Lee Hua Li Xianlei Li Dion Zhanyun Koh Yuwen Wang Tong Ming Liu Zheng Yang Chwee Teck Lim Christine Cheung Andy Tay | 2023 | Bioactive Materials2023，，6： | 0 |
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