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| 1 | Predictive value of Ki67 and p53 in locally advanced rectal cancer:Correlation with thymidylate synthase and histopathological tumor regression after neoadjuvant 5-FU-based chemoradiotherapy显示文摘AIM: To investigate the predictive value of Ki67 and p53 and their correlation with thymidylate synthase (TS) gene expression in a rectal cancer patient cohort treated according to a standardized recommended neoadjuvant treatment regimen.METHODS: Formalin fixed, paraffin embedded pre-therapeutical tumor biopsies (n = 22) and post-therapeutical resection specimens (n = 40) from patients with rectal adenocarcinoma (clinical UICC stage Ⅱ/Ⅲ) receiving standardized neoadjuvant 5-fluorouracil (5-FU) based chemoradiotherapy were studied for Ki67 and p53 expression by immunohistochemistry and correlated with TS mRNA expression by quantitative TaqMan real-time PCR after laser microdissection. The results were compared with histopathological tumor regression according to a standardized semiquantitative score grading system.RESULTS: Responders (patients with high tumor regression) showed a significantly lower Ki67 expression than non-responders in the pre-therapeutical tumor biopsies (81.2% vs 16.7%; P < 0.05) as well as in the post-therapeutical resection specimens (75.8% vs 14.3%; P < 0.01). High TS mRNA expression was significantly correlated with a high Ki67 index and low TS mRNA expression was significantly correlated with a low Ki67 index in the pre-therapeutical tumor biopsies (corr. coef. = 0.46; P < 0.01) as well as in the post-therapeutical resection specimens (corr. coef. = 0.40; P < 0.05). No significant association was found between p53 and TS mRNA expression or tumor regression.CONCLUSION: Ki67 has, like TS, predictive value in rectal cancer patients after neoadjuvant 5-FU based chemoradiotherapy. The close correlation between Ki67 and TS indicates that TS is involved in active cell cycle processes. | Christiane Jakob Torsten Liersch Wolfdietrich Meyer Heinz Becker Gustavo B Baretton Daniela E Aust | 2008 | World Journal of Gastroenterology2008，14，7： | 29 |
| 2 | Ephrin-B2 is differentially expressed in the intestinal epithelium in Crohn's disease and contributes to accelerated epithelial wound healing in vitro显示文摘AIM: Eph receptor tyrosine kinases and their membrane bound receptor-like ligands, the ephrins, represent a bi-directional cell-cell contact signaling system that directs epithelial movements in development. The meaning of this system in the adult human gut is unknown. We investigated the Eph/ephrin mRNA expression in the intestinal epithelium of healthy controls and patients with inflammatory bowel disease (IBD).METHODS: mRNA expression profiles of all Eph/ephrin family members in normal small intestine and colon were established by real-time RT-PCR. In addition, differential expression in IBD was investigated by cDNA array technology, and validated by both real-time RT-PCR and immunohistochemistry. Potential effects of enhanced EphB/ephrin-B signaling were analyzed in an in vitro IEC-6 cell scratch wound model.RESULTS: Human adult intestinal mucosa exhibits a complex pattern of Eph receptors and ephrins. Beside the known prominent co-expression of EphA2 and ephrinA1,we found abundantly co-expressed EphB2 and ephrin-B1/2.Interestingly, cDNA array data, validated by real-time PCR and immunohistochemistry, showed upregulation of ephrin-B2 in both perilesional and lesional intestinal epithelial cells of IBD patients, suggesting a role in epithelial homeostasis. Stimulation of ephrin-B signaling in ephrinB1/2 expressing rat IEC-6-cells with recombinant EphB1Fc resulted in a significant dose-dependent acceleration of wound closure. Furthermore, fluorescence microscopy showed that EphB1-Fc induced coordinated migration of wound edge cells is associated with enhanced formation of lamellipodial protrusions into the wound, increased actin stress fiber assembly and production of laminin at the wound edge.CONCLUSION: EphB/ephrin-B signaling might represent a novel protective mechanism that promotes intestinal epithelial wound healing, with potential impact on epithelial restitution in IBD. | Christian Hafner Stefanie Meyer Thomas Langmann Gerd Schmitz Frauke Bataille Ilja Hagen Bernd Becker Alexander Roesch Gerhard Rogler Michael Landthaler Thomas Vogt | 2005 | World Journal of Gastroenterology2005，11，26： | 9 |
| 3 | Dual Energy CT Characterization of Urinary Calculi: Initial In Vitro and Clinical Experience显示文摘 | Anno Graser Thorsten R. C. Johnson Markus Bader Michael Staehler Nicolas Haseke Konstantin Nikolaou Maximilian F. Reiser Christian G. Stief Christoph R. Becker | 2008 | Investigative Radiology2008，，2： | 3 |
| 4 | Neoadjuvant chemotherapy with cisplatin, 5-FU, and leucovorin (PLF) in locally advanced gastric cancer: a prospective phase II study显示文摘 | Katja Ott Andreas Sendler Karen Becker Hans-Joachim Dittler Hermann Helmberger Raimonde Busch Christian Kollmannsberger J. Rüdiger Siewert Ulrich Fink | 2003 | Gastric Cancer2003，，3： | 3 |
| 5 | Consensus and Future Directions on the Definition of High On-Treatment Platelet Reactivity to Adenosine Diphosphate显示文摘 | Laurent Bonello Udaya S. Tantry Rossella Marcucci Ruediger Blindt Dominick J. Angiolillo Richard Becker Deepak L. Bhatt Marco Cattaneo Jean Philippe Collet Thomas Cuisset Christian Gachet Gilles Montalescot Lisa K. Jennings Dean Kereiakes Dirk Sibbing Die | 2010 | Journal of the American College of Cardiology2010，，12： | 3 |
| 6 | Mutation of C. elegans demethylase spr-5 extends transgenerational longevity显示文摘象长寿那样的复杂 organismal 性质能被非基因的因素越过代播送。这里,我们表明 C 的那删除。elegans histone H3 离氨酸 4 dimethyl (H3K4me2 ) demethylase, spr-5,在 lifespan 引起 trans 辈份的增加。我们识别一个修改染色质的网络,它调整这 lifespan 扩展。我们进一步证明这 trans 辈份的 lifespan 延期依赖于包含类固醇 dafachronic 酸的一条神经质的发信号小径,原子受体 DAF-12 的使活跃之物。这些调查结果建议 demethylase SPR-5 的损失引起 H3K4me2 表明小径的已知的调整 lifespan 的 mis 规定和激活,导致 trans 辈份的 lifespan 扩展。 | Eric Lieberman Greer Ben Becker Christian Latza Adam Antebi Yang Shi | 2016 | Cell Research2016，26，2： | 3 |
| 7 | Argon Plasma Coagulation of Cervical Heterotopic Gastric Mucosa as an Alternative Treatment for Globus Sensations显示文摘 | Monther Bajbouj Valentin Becker Florian Eckel Stephan Miehlke Oliver Pech Christian Prinz Roland M. Schmid Alexander Meining | 2009 | Gastroenterology2009，，2： | 2 |
| 8 | Preoperative chemoradiotherapy and postoperative chemotherapy with fluorouracil and oxaliplatin versus fluorouracil alone in locally advanced rectal cancer: initial results of the German CAO/ARO/AIO-04 randomised phase 3 trial显示文摘 | Claus R?del Torsten Liersch Heinz Becker Rainer Fietkau Werner Hohenberger Torsten Hothorn Ullrich Graeven Dirk Arnold Marga Lang-Welzenbach Hans-Rudolf Raab Heiko Sülberg Christian Wittekind Sergej Potapov Ludger Staib Clemens Hess Karin Weigang-K?hler G | 2012 | Lancet Oncology2012，，7： | 2 |
| 9 | Material differentiation by dual energy CT: initial experience显示文摘 | Thorsten R. C. Johnson Bernhard Krau? Martin Sedlmair Michael Grasruck Herbert Bruder Dominik Morhard Christian Fink Sabine Weckbach Miriam Lenhard Bernhard Schmidt Thomas Flohr Maximilian F. Reiser Christoph R. Becker | 2007 | European Radiology2007，，6： | 2 |
| 10 | Ephrin-B reverse signaling induces expression of wound healing associated genes in IEC-6 intestinal epithelial cells显示文摘AIM: Eph receptors and ephrin ligands play a pivotal role in development and tissue maintenance. Since previous data have indicated an involvement of ephrin-B2 in epithelial healing, we investigated the gene expression and downstream signaling pathways induced by ephrin-B mediated cell-cell signaling in intestinal epithelial cells.METHODS: Upon stimulation of ephrin-B pathways in IFC-6 cells with recombinant rat EphB1-Fc, gene expression was analyzed by Affymetrix(R) rat genome 230 high density arrays at different time points. Differentially expressed genes were confirmed by real-time RT-PCR. In addition, MAP kinase pathways and focal adhesion kinase (FAK) activation downstream of ephrin-B were investigated by immunoblotting and fluorescence microscopy.RESULTS: Stimulation of the ephrin-B reverse signaling pathway in IEC-6 cells induces predominant expression of genes known to be involved into wound healing/cell migration, antiapoptotic pathways, host defense and inflammation. Cox-2, c-Fos, Egr-1, Egr-2, and MCP-1 were found among the most significantly regulated genes.Furthermore, we show that the expression of repairrelated genes is also accompanied by activation of the ERK1/2 MAP kinase pathway and FAK, two key regulators of epithelial restitution.CONCLUSION: Stimulation of the ephrin-B reverse signaling pathway induces a phenotype characterized by upregulation of repair-related genes, which may partially be mediated by ERK1/2 pathways. | Christian Hafner Stefanie Meyer Ilja Hagen Bernd Becker Alexander Roesch Michael Landthaler Thomas Vogt | 2005 | World Journal of Gastroenterology2005，11，29： | 2 |
| 11 | Prediction of survival after liver transplantation by pre-transplant parameters显示文摘 | Tobias J. Weismüller Jana Prokein Thomas Becker Hannelore Barg-Hock Jürgen Klempnauer Michael P. Manns Christian P. Strassburg | 2008 | Scandinavian Journal of Gastroenterology2008，，6： | 1 |
| 12 | A survey on problems and methods in generalized assembly line balancing 显示文摘 | Christian Becker Armin Scholl | 2006 | European Journal of Operational Research2006，168，5： | 1 |
| 13 | A survey on problems and methods in generalized assembly line balancing 显示文摘 | Christian Becker Amain Scholl | 2006 | European Journal of Operation Research2006，，168： | 1 |
| 14 | Soluble intercellular adhesion molecule- 1 inhibits MHC- restricted Spcitfic T cell/tumor interaction显示文摘 | Christian T Schmidt R | 1993 | J Immunol1993，151，12： | 1 |
| 15 | Adenocarcinomas of the Esophagogastric Junction Are More Likely to Respond to Preoperative Chemotherapy than Distal Gastric Cancer显示文摘 | Daniel Reim Ralf Gertler Alexander Novotny Karen Becker Christian Büschenfelde Matthias Ebert Martin Dobritz Rupert Langer Heinz Hoefler Helmut Friess Christoph Schumacher | 2012 | Annals of Surgical Oncology2012，，7： | 1 |
| 16 | Indications for and Outcomes After Combined Lung and Liver Transplantation: A Single-Center Experience on 13 Consecutive Cases显示文摘 | Gerrit Grannas Michael Neipp Marius M. Hoeper Jens Gottlieb Rainer Lück Thomas Becker Andre Simon Christian P. Strassburg Michael P. Manns Tobias Welte Axel Haverich Jürgen Klempnauer Bj?rn Nashan Martin Strueber | 2008 | Transplantation2008，，4： | 1 |
| 17 | Outcome and quality of life in patients with polycystic liver disease after liver or combined liver‐kidney transplantation显示文摘 | Gabriele I. Kirchner Kinan Rifai Tobias Cantz Bjoern Nashan Christoph Terkamp Thomas Becker Christian Strassburg Hannelore Barg‐Hock Siegfried Wagner Rainer Lück Juergen Klempnauer Michael P. Manns | 2006 | Liver Transpl2006，，8： | 1 |
| 18 | Assessing the Impact of Ischaemia Time During Partial Nephrectomy <ce:link locator='eulogo1'/>显示文摘 | Frank Becker Hein Van Poppel Oliver W. Hakenberg Christian Stief Inderbir Gill Giorgio Guazzoni Francesco Montorsi Paul Russo Michael St?ckle | 2009 | European Urology2009，，4： | 1 |
| 19 | Substrates and Regulation Mechanisms for the Human Mitochondrial Sirtuins Sirt3 and Sirt5显示文摘 | Christine Schlicker Melanie Gertz Panagiotis Papatheodorou Barbara Kachholz Christian F.W. Becker Clemens Steegborn | 2008 | Journal of Molecular Biology2008，，3： | 1 |
| 20 | Validation of extraction methods for total RNA and miRNA from bovine blood prior to quantitative gene expression analyses 显示文摘 | Hammerle-Fickinger Andrea Riedmaier Irmgard Becker Christiane | 2010 | Biotechnology Letters2010，32，1： | 1 |
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