|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cationic nanocarriers induce cell necrosis through impairment of Na^+/K^+-ATPase and cause subsequent inflammatory response显示文摘有积极表面费用的 Nanocarriers 以他们限制了他们的临床的应用的毒性著名。位于他们的毒性下面的机制例如煽动性的反应的正式就职,仍然保持大部分未知。在我们发现了 cationic nanocarriers 的那注射的现在的学习,包括 cationic liposomes, PEI,和 chitosan,导致了坏死的房间的快速的外观。cationic nanocarriers 导致的房间坏死依赖于他们的积极表面费用,但是不要求 RIP1 和 Mlkl。相反,细胞内部的 Na + 超载被发现伴随房间死亡。在文化媒介或有 Na +/K+-ATPase 阳离子绑定地点禁止者 ouabain 的房间的预告的处理的 Na + 的弄空,从房间坏死的保护的房间。而且,有 cationic nanocarriers 的处理在 vitro 并且在 vivo 禁止了 Na +/K+-ATPase 活动。计算模拟证明 cationic 搬运人能与 Na +/K+-ATPase 的阳离子绑定地点交往。有 ouabain 的小剂量的老鼠 pretreated 在 cationic nanocarriers 的致命的剂量的注射以后显示出改进幸存。进一步的分析建议 cationic nanocarriers 和 mitochondrial DNA 的产生的漏导致的那房间坏死能在 vivo 触发严重发炎,它被包含 TLR9 并且 MyD88 发信号的一条小径调停。一起拿,我们的结果揭示由此的新奇机制 cationic nanocarriers 通过和 mitochondrial 的随后的暴露的和 Na +/K+-ATPase, 的相互作用导致尖锐房间坏死是的联系损坏的分子的模式调停的一个关键事件煽动性的回答。我们的学习为评估 nanocarriers 的 biocompatibility 并且为药交货设计更好、更安全的有重要含意。 | Xiawei Wei Bin Shao Zhiyao He Tinghong Ye Min Luo Yaxiong Sang Xiao Liang Wei Wang Shuntao Luo Shengyong Yang Shuang Zhang Changyang Gong Maling Gou Hongxing Deng Yinglan Zhao Hanshuo Yang Senyi Deng Chengjian Zhao Li Yang Zhiyong Qian Jiong Li Xun Sun Jiahuai Han Chengyu Jiang Min Wu Zhirong Zhang | 2015 | Cell Research2015，25，2： | 21 |
| 2 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 3 | Immunomodulatory effects of polysaccharides from edible fungus:a review显示文摘The polysaccharides from edible fungus showed many kinds of biological activities,including anti-tumor,immunomodulatory,anti-inflammatory,anti-diabetes,improving functional constipation activities.In particular,the immunomodulatory effects have been paid more and more attention by scholars,but there was no systematic introduction of their immunomodulatory mechanism.So,this review introduced the immunomodulatory effects and mechanism of edible fungus polysaccharides in recent years,and then the relationships between structure and immunomodulatory effect were also discussed. | Zhenghua Yin Zhenhua Liang Changqin Li Jinmei Wang Changyang Ma Wenyi Kang | 2021 | Food Science and Human Wellness2021，10，4： | 12 |
| 4 | Highly efficient base editing in human tripronuclear zygotes显示文摘 | Changyang Zhou Meiling Zhang Yu Wei Yidi Sun Yun Sun Hong Pan Ning Yao Wanxia Zhong Yixue Li Weiping Li3 Hui Yang Zi-jiang Chen | 2017 | Protein & Cell2017，8，10： | 6 |
| 5 | Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method显示文摘This study was designed to elucidate the immunoregulation of Ganoderma lucidum.HPLC fingerprint and spectrum-effect relationship of G.lucidum were established to predict the active compounds and BP neural network model was established to predict the efficacy.Then the target compounds were identified by high resolution mass spectrometry.The results indicated that there are both enhanced immunity and immunosuppressive components in G.lucidum.BP neural network was trained with the common peak area and immune effi cacy index of G.lucidum fi ngerprint as samples,and a combined evaluation system of G.lucidum fi ngerprint effi cacy was established.The correlation coeffi cient R of BP network model was 0.98643,and the error of pharmacodynamic prediction results was in the ideal range.Eight compounds were identifi ed by high resolution mass spectrometry.The compounds related to immune activity in G.lucidum were determined in this study. | Changqin Li Yiping Cui Jie Lu Lijun Meng Changyang Ma Zhenhua Liu Yan Zhang Wenyi Kang | 2021 | Food Science and Human Wellness2021，10，3： | 6 |
| 6 | Nanomaterials for radiotherapeutics-based multimodal synergistic cancer therapy显示文摘Over the past decade,numerous studies have attempted to enhance the effectiveness of radiotherapy(external beam radiotherapy and internal radioisotope therapy)for cancer treatment.However,the low radiation absorption coefficient and radiation resistance of tumors remain major critical challenges for radiotherapy in the clinic.With the development of nanomedicine,nanomaterials in combination with radiotherapy offer the possibility to improve the efficiency of radiotherapy in tumors.Nanomaterials act not only as radiosensitizers to enhance radiation energy,but also as nanocarriers to deliver therapeutic units in combating radiation resistance.In this review,we discuss opportunities for a synergistic cancer therapy by combining radiotherapy based on nanomaterials designed for chemotherapy,photodynamic therapy,photothermal therapy,gas therapy,genetic therapy,and immunotherapy.We highlight how nanomaterials can be utilized to amplify antitumor radiation responses and describe cooperative enhancement interactions among these synergistic therapies.Moreover,the potential challenges and future prospects of radio-based nanomedicine to maximize their synergistic efficiency for cancer treatment are identified. | Xi Yang Ling Gao Qing Guo Yongjiang Li Yue Ma Ju Yang Changyang Gong Cheng Yi | 2020 | Nano Research2020，13，10： | 3 |
| 7 | Recent advances in chemical durability and mechanical stability of superhydrophobic materials: Multi-strategy design and strengthening显示文摘Inspired by the lotus leaf effect, non-wetting artificial superhydrophobic surfaces demonstrate an enormous potential in numerous fields. However, limited by poor stability and durability, superhydrophobic surfaces are rarely available for practical applications. In this review, based on the wettability mechanisms and failure modes of superhydrophobic surfaces, it is proposed that the construction of highly stable superhydrophobic materials can be approached from four aspects, including structural design, chemical bonding, interfacial-strengthening of hydrophobic materials and substrates, and self-healing. We introduced in detail the design ideas, strengthening approaches, and characterization tools of highly stable superhydrophobic materials from the perspective of multi-strategy design and strengthening, and provided corresponding insights. Eventually, the development, current status, and prospects of highly stable and multifunctional superhydrophobic materials were also presented in detail. Recent advances and development prospects of durable superhydrophobic materials were summarized and discussed in this review,providing certain insights and design guidelines for the fabrication of stable superhydrophobic materials. | Peng Wang Changyang Li Dun Zhang | 2022 | Journal of Materials Science & Technology2022，，34： | 3 |
| 8 | A Retrospective Study of Treatment Efficacy of 65 Children with Ametroic Amblyopia显示文摘Purpose: To investigate relationship between treatment efficacy and the severity of ametropic amblyopia, the type of anisometropia and patient age. Methods:A total of 65 children with ametropic amblyopia undergoing clinical treatment in the ophthalmology department of Shaoguan Hygienic Hospital of Women and Children between June 2005 and November 2011 were enrolled in this study. The treatment efficacy for those subjects with different severities of ametropic amblyopia, types of anisometropia and ages was recorded. Results: The near-recovery/recovery rate, improvement rate and ineffectiveness rate were 70.8%, 16.9% and 12.3%, respectively. The recovery rates in mild-, moderate- and severe amblyopia groups were 97.1%, 61.1% and 8.3% respectively (P<0.05)..The recovery rate for patients with hyperopic-, astigmatic- and myopic anisometropia were 75.8%, 78.3% and 33.3% respectively. The recovery rates did not differ between hyperopic- and astigmatic- anisometropia children(P>0.05), whereas a statistically significant difference was noted between myopic children compared with their hyperopic and myopic-anisometropia counterparts (all P<0.05). The recovery rate for patients aged 3 to 6 years was 86.8% and 48.1% for those aged between 7 and 10 years (P<0.05). Conclusion: The treatment efficacy in ametropic amblyopia is associated with the severity of amblyopia, type of anisometropia and patient's age. Older patients with more severe amblyopia had poorer treatment efficacy. The efficacy in patients with hyperopic and astigmatic anisometropia was better than that for myopic anisometropia subjects. | Yuxiang Zhang Lihua Huang Changyang Cao Yingfang Li | 2012 | Eye Science2012，27，2： | 2 |
| 9 | Synthesis and characterization of PEG-PCL-PEG thermosensitive hydrogel显示文摘 | ChangYang Gong Shuai Shi PengWei Dong Bing Kan MaLing Gou XianHuo Wang XingYi Li Feng Luo Xia Zhao YuQuan Wei ZhiYong Qian | 2008 | International Journal of Pharmaceutics2008，，1： | 1 |
| 10 | A novel injectable local hydrophobic drug delivery system: Biodegradable nanoparticles in thermo-sensitive hydrogel显示文摘 | MaLing Gou XingYi Li Mei Dai ChangYang Gong XianHuo Wang Yao Xie HongXin Deng LiJuan Chen Xia Zhao ZhiYong Qian YuQuan Wei | 2008 | International Journal of Pharmaceutics2008，，1： | 1 |
| 11 | Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice显示文摘We here report a genome-editing strategy to correct spinal muscular atrophy(SMA).Rather than directly targeting the pathogenic exonic mutations,our strategy employed Cas9 and guide-sg RNA for the targeted disruption of intronic splicing-regulatory elements.We disrupted intronic splicing silencers(ISSs,including ISS-N1 and ISS+100)of survival motor neuron(SMN)2,a key modifier gene of SMA,to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs.Survival of splicing-corrected iPSC-derived motor neurons was rescued with SMN restoration.Furthermore,co-injection of Cas9 mRNA from Streptococcus pyogenes(SpCas9)or Cas9 from Staphylococcus aureus(SaCas9)alongside their corresponding sgRNAs targeting ISS-N1 into zygotes rescued 56%and 100%of severe SMA transgenic mice(Smn-/-,SMN2tg/-).The median survival of the resulting mice was extended to>400 days.Collectively,our study provides proof-of-principle for a new strategy to therapeutically intervene in SMA and other RNA-splicing-related diseases. | Jin-Jing Li Xiang Lin Cheng Tang Ying-Qian Lu Xinde Hu Erwei Zuo He Li Wenqin Ying Yidi Sun Lu-Lu Lai Hai-Zhu Chen Xin-Xin Guo Qi-Jie Zhang Shuang Wu Changyang Zhou Xiaowen Shen Qifang Wang Min-Ting Lin Li-Xiang Ma Ning Wang Adrian R.Krainer Linyu Shi Hui Yang Wan-Jin Chen | 2020 | National Science Review2020，7，1： | 1 |
| 12 | Preparation of polystyrene-poly(vinyl alcohol) double shell显示文摘 | LI Bo ZHANG Zhanwen WANG Changyang | 2003 | Proceedings of the SHE2003，5228，72： | 1 |
| 13 | Bioinspired therapeutic platform based on extracellular vesicles for prevention of arterial wall remodeling in hypertension显示文摘Arterial stiffness due to the vessel remodeling is closely linked to raised blood pressure,and its physiopathologic mechanism is still not fully understood.We here aimed to explore whether extracellular vesicle(EV)mediated intercellular communication between endothelium and smooth muscle cell contribute to the blood vessel remodeling under hypertension.We here revealed that the arterial endothelial cells robustly secreted EV,which in turn could be circulated and/or directly taken up by the subendothelial smooth muscle cells(SMC).Under hypertension,the EV secretion increased and the miRNA profile changed significantly mainly due to the raised mechanical force and subsequent enhanced reactive oxygen species generation.Among the miRNA cargos in the EV,miR-320d/423-5p were found increased most significantly.In vivo delivery of miR-320d/423-5p mimics via engineered EV increased their expression in arterial vessels,recapitulating the phenotype in hypertension.In contrast,therapeutic delivery of miR-320d/423-5p inhibitors via engineered EV alleviated the phenotype in spontaneous hypertension rat model.Together,we have found that the injured endothelium due to the raised mechanical force in hypertension contributes to the arterial wall remodeling via the secreted EV.Our study has not only provided novel insights on the mechanism of hypertension associated blood vessel wall remodeling,but also shed light on therapeutic intervention of hypertension associated vascular diseases. | Chen Wang Changyang Xing Zhelong Li Yunnan Liu Qiaoying Li Yixiao Wang Jiao Hu Lijun Yuan Guodong Yang | 2022 | Bioactive Materials2022，7，2： | 1 |
| 14 | Allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci using Staphylococcus aureus Cas9(SaCas9)显示文摘Allele-specific DNA methylation is the most important imprinting marker localized to differentially methylated regions(DMRs),and aberrant genomic imprinted DNA methylation is associated with some human diseases,including Prader-Willi syndrome and cancer.Thus,the development of an effective strategy for the precise editing of allele-specific methylated genes is essential for the functional clarification of imprinting elements and the correction of imprinting disorders in human diseases.To discover a feasible allele-specific genome editing tool based on the CRISPR/Cas system,which is an efficient genetargeting technique in various organisms,we examined the targeting efficiency of Staphylococcus aureus Cas9(SaCas9)and Streptococcus pyogenes Cas9(SpCas9)in response to DNA methylation interference.We found that the targeting efficiency of SaCas9,but not SpCas9,was enhanced by targeted DNA demethylation using the d Cas9-Tet1 catalytic domain(CD)but suppressed by targeted DNA methylation using Dnmt3l-Dnmt3a-d Cas9.An in vitro cleavage assay further demonstrated that SaCas9 nuclease activity was inhibited by 5-methylcytosine(5mC)in a synthesized Cp G-containing context.Further analysis with Ch IP-Q-PCR demonstrated that the non-methylated sequence targeting of Sa Cas9 depends on the binding preference of SaCas9 to non-methylated sequences.Taking advantage of this feature of SaCas9,we have successfully obtained non-methylated allele-biased targeted embryos/mice for two imprinting genes,H19 and Snrpn,with relatively high efficiencies of 28.6%and 47.4%,respectively.These results indicate that the targeting efficiency of SaCas9 was strongly reduced by DNA methylation.By using SaCas9,we successfully achieved allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci. | Yajing Liu Jianan Li Changyang Zhou Bin Meng Yu Wei Guang Yang Zongyang Lu Qingmei Shen Yu Zhang Hui Yang Yunbo Qiao | 2019 | Science Bulletin2019，64，21： | 1 |
| 15 | A Likelihood andLocal Constraint Level Set Model for Liver Tumor Segmenta-tion from CT Volumes显示文摘 | Li Changyang Wang Xiuying Eberl S | 2013 | IEEE Transactions on BiomedicalEngineering2013，60，10： | 1 |
| 16 | Recent advances in enhancing reactive oxygen species based chemodynamic therapy显示文摘Chemodynamic therapy(CDT),defined as an in situ oxidative stress response catalyzed by the Fenton or Fenton-like reactions to generate cytotoxic hydroxyl radicals(•OH)at tumor sites,exhibits conspicuous inhibition of tumor growth.It has attracted extensive attention for its outstanding edge in effectiveness,lower systemic toxicity and side effects,sustainability,low cost and convenience.However,the inconfor-mity of harsh Fenton reaction conditions and tumor microenvironment hamper its further development,based on which,numerous researchers have made efforts in further improving the efficiency of CDT.In this review,we expounded antitumor capacity of CDT in mechanism,together with its limitation,and then summarized and came up with several strategies to enhance CDT involved tumor therapy strategies by 1)improving catalytic efficiency;2)increasing hydrogen peroxide levels at tumor sites;3)reducing glutathione levels at tumor sites;4)applying external energy intervention;5)amplifying the distribu-tion of hydroxyl radicals at tumor sites;and 6)combination therapy.Eventually,the perspectives and challenges of CDT are further discussed to encourage more in-depth studies and rational reflections. | Xinchao Li Rui Luo Xiuqi Liang Qinjie Wu Changyang Gong | 2022 | Chinese Chemical Letters2022，33，5： | 1 |
| 17 | A likelihood and local constraint level set model for liver tumor segmen- tation from CT volurnes显示文摘 | Li Changyang Wang Xiuying EBERL S | 2013 | IEEE Transactions on Biomedical Engineering2013，60，10： | 1 |
| 18 | Synthesis and characterization of PEG-PCL-PEG thermosensitive hydrogel显示文摘 | ChangYang Gong Shuai Shi PengWei Dong Bing Kan MaLing Gou XianHuo Wang XingYi Li Feng Luo Xia Zhao YuQuan Wei ZhiYong Qian | 2008 | International Journal of Pharmaceutics2008，，1： | 1 |
| 19 | A comprehensive review on mechanism of natural products against Staphylococcus aureus显示文摘The abuse of antibiotics seriously threatens the therapeutic effect.Natural products from plants,antimicrobial peptides and micro-organisms have antibacterial activity against Staphylococcus aureus.The mechanism of natural products reported in recent years on S.aureus includes six types,which are the acting on bacterial cell wall,cytomembrane,metabolic pathway,protein,nucleic acid,biofilm and quorum sensing system to achieve antibacterial effect.This article summarized the mechanism of natural products reported in recent years on S.aureus.It is found that the natural products acting on cell wall or cell wall synthesis pathway key enzymes are relatively safe and highly selective.It has the prospect of developing into medicine and provides the clue to investigate and utilize natural products in antibacterial activity. | Yingying Zhao Jinfeng Wei Changqin Li Adel F.Ahmed Zhenhua Liu Changyang Ma | 2022 | Journal of Future Foods2022，2，1： | 0 |
| 20 | A tactical nanomissile mobilizing antitumor immunity enables neoadjuvant chemo-immunotherapy to minimize postsurgical tumor metastasis and recurrence显示文摘Neoadjuvant chemotherapy has become an indispensable weapon against high-risk resectable cancers,which benefits from tumor downstaging.However,the utility of chemotherapeutics alone as a neoadjuvant agent is incapable of generating durable therapeutic benefits to prevent postsurgical tumor metastasis and recurrence.Herein,a tactical nanomissile(TALE),equipped with a guidance system(PD-L1 monoclonal antibody),ammunition(mitoxantrone,Mit),and projectile bodies(tertiary amines modified azobenzene derivatives),is designed as a neoadjuvant chemo-immunotherapy setting,which aims at targeting tumor cells,and fast-releasing Mit owing to the intracellular azoreductase,thereby inducing immunogenic tumor cells death,and forming an in situ tumor vaccine containing damage-associated molecular patterns and multiple tumor antigen epitopes to mobilize the immune system.The formed in situ tumor vaccine can recruit and activate antigen-presenting cells,and ultimately increase the infiltration of CD8^(+)T cells while reversing the immunosuppression microenvironment.Moreover,this approach provokes a robust systemic immune response and immunological memory,as evidenced by preventing 83.3%of mice from postsurgical metastasis or recurrence in the B16-F10 tumor mouse model.Collectively,our results highlight the potential of TALE as a neoadjuvant chemo-immunotherapy paradigm that can not only debulk tumors but generate a long-term immunosurveillance to maximize the durable benefits of neoadjuvant chemotherapy. | Tao He Mingxing Hu Shunyao Zhu Meiling Shen Xiaorong Kou Xiuqi Liang Lu Li Xinchao Li Miaomiao Zhang Qinjie Wu Changyang Gong | 2023 | Acta Pharmaceutica Sinica B2023，13，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员