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| 1 | The adult human testis transcriptional cell atlas显示文摘Human adult spermatogenesis balances spermatogonial stem cell (SSC) self-renewal and differentiation,alongside complex germ cell-niche interactions,to ensure long-term fertility and faithful genome propagation. Here,we performed single-cell RNA sequencing of -6500 testicular cells from young adults. We found five niche/somatic cell types (Leydig,myoid,Sertoli,endothelial,macrophage),and observed germline-niche interactions and key human-mouse differences. Spermatogenesis,including meiosis,was reconstructed computationally,revealing sequential coding,non-coding,and repeat-element transcriptional signatures. Interestingly,we identified five discrete transcriptional/developmental spermatogonial states,including a novel early SSC state,termed State 0. Epigenetic features and nascent transcription analyses suggested developmental plasticity within spermatogonial States. To understand the or丨gin of State 0,we profiled testicular cells from infants,and identified distinct similarities between adult State 0 and infant SSCs. Overall,our datasets describe key transcriptional and epigenetic signatures of the normal adult human testis,and provide new insights into germ cell developmental transitions and plasticity. | Jingtao Guo Edward J. Grow Hana Mlcochova Geoffrey J. Maher Cecilia Lindskog Xichen Nie Yixuan Guo Yodai Takei Jina Yun Long Cai Robin Kim Douglas T. Carrell Anne Goriely James M. Hotaling Bradley R. Cairns | 2018 | Cell Research2018，28，12： | 12 |
| 2 | Photolysis of benzophenone with two-step two-laser excitation显示文摘The first laser excites a molecule to a lower triplet state and another sequential laser excites it resonantly to higher triplet states or makes it ionization. This two-step two-laser method provides a novel way to study the electron transfer or charge transfer of excited molecules. The higher excited benzophenone and its radical cation can be observed under the time-resolved absorption method when it was excited to lower triplet state by one laser and another one exciting it to higher triplet states resonantly. The higher excited benzophenone molecules undergoing inter-molecular hydrogen abstraction with iso-propylalcohol molecules are faster than the lower ones. | CAI Xichen HAN Zhenhui , YAO Side& LIN NianyunLaboratory of Radiation Chemistry, Shanghai Institute of Nuclear Research, Chinese Academy of Sciences, Shanghai 201800, China | 2001 | Science China Chemistry2001，44，6： | 1 |
| 3 | Photophysical and photochemical processes of riboflavin (vitamin B2) by means of the transient absorption spectra in aqueous solution显示文摘 | Changyuan Lu Zhenhui Han Guanshu Liu Xichen Cai Yuling Chen Side Yao | 2001 | Science in China Series B: Chemistry2001，，1： | 1 |
| 4 | Photochemical Photobiological Sciences 显示文摘 | Kelechi C A Cai Xichen Douglas C N | 2008 | 7 ( 12 ) : 1469- 14722008，7，12： | 1 |
| 5 | Viral RNA-based transfection and expression of enhanced green fluorescent protein in the parasitic protozoan Eimeria stiedae显示文摘Eimeria stiedae(E.stiedae),the most common protozoan pathogen of rabbits,causes coccidiosis.This disease is problematic for rabbit breeders [1].There is little information about the cellular and molecular biology of this pathogen and a lack of genetic tools.Genetic manipulation is necessary to advance our understanding of parasite biology and ultimately develop vaccines and treat diseases.DNA-mediated and viral RNA-mediated transfection systems have successfully been established in many protozoan parasites [2,3].It was discovered that E.stiedae possesses a dsRNA virus(EsRV1),and more recently,the genome of this virus has been sequenced.To date,however,there is no report on the use of viral RNA-mediated transfection for E.stiedae.In this study,we report a transient viral RNA-mediated transfection of E.stiedae using enhanced green fluorescent protein(EGFP)as a reporter gene.Our study provides a valuable genetic tool for studying the biology of E.stiedae. | Caiyan Xin Jianhua Li Pengtao Gong Bin Wu Nan Zhang Ju Yang Xuepeng Cai Xichen Zhang | 2019 | Acta Biochimica et Biophysica Sinica2019，51，2： | 0 |
| 6 | Analysis of products of thymine irradiated by ^(18)O^(8+) ion beam in N_2O saturated aqueous solution显示文摘Some methods of capillary gas chromatography, such as GC, GC-MS,GC-FT-IR, are used to analyze the products of thymine irradiated by 18O8+ ion beam in N2O saturated aqueous solution. From the results of GC-MS the molecular weight of products can be determined, and from the results of GC-FT-IR some molecular structure information of products can be obtained. By this way the products,5,6-Dihydrothyndne, 5-Hydroxyl-5-Methylhydantoin, 5-Hydroxyl-6-Hydrothymine, 5-Hydro-6-Hydroxylthymine, 5-Hydroxymethyluracil, Trans-Thymine glycol, Cis-Thymine glycol and dimers are determined without separation of them from samples. Though these products are as same as those products of thymine irradiated by γ rays in N2O saturated aqueous solution, the mechanism of thymine irradiated by heavy ion beam in aqueous solution is different from that by γ rays. The main products of thymine irradiated by 18O8+ ion beam in N2O saturated aqueous solution are hydroxyl adducts at 5-6 band of thymine, while the main products of thymine irradiated by γ ray in N2O saturated aqueous solution are dimers of thymine. | CAI Xichen WEI Zengquan LI Wenjian LIANG Jianping LI Qiang (Institute of Modern Physics, the Chinese Academy of Sciences, Lanzhou 730000) | 1999 | Nuclear Science and Techniques1999，10，3： | 0 |
| 7 | Progress of IMP Laser Ion Source显示文摘Progress of IMP Laser Ion SourceProgressofIMPLaserIonSource￥ZhaoZhizheng;YangYongfengandCaiXichenLotsOfprogressesofIMPlaserio... | Zhao Zhizheng Yang Yongfeng and Cai Xichen | 1995 | IMP & HIRFL Annual Report1995，，0： | 0 |
| 8 | Stepwise Resonant Excitation and Photoionization of ^(169)Tm显示文摘StepwiseResonantExcitationandPhotoionizationof^(169)TmStepwiseResonantExcitationandPhotoionizationof^(169)Tm￥YangYOngfeng;Zhao... | Yang YOngfeng Zhao Zhizheng and Cai Xichen | 1995 | IMP & HIRFL Annual Report1995，，0： | 0 |
| 9 | Design of a Stable AtomicSource and Ionizers显示文摘Design of a Stable Atomic Source and IonizersDesignofaStableAtomicSourceandIonizers￥CaiXichen,YangYongfengamdZhaoZhizhengForo... | Cai Xichen, Yang Yongfeng amd Zhao Zhizheng | 1995 | IMP & HIRFL Annual Report1995，，0： | 0 |
| 10 | Optical Detector Used for Measuring CVL Beam Pulse Properties 显示文摘Optical Detector Used for Measuring CVL Beam Pulse Properties￥ZhuShaofei;ZhaoZhizheng;YangYongfengandCaiXichenBecauselaserion... | Zhu Shaofei Zhao Zhizheng Yang Yongfeng and Cai Xichen | 1994 | IMP & HIRFL Annual Report1994，，0： | 0 |
| 11 | Status of Studying Laser Ion Source显示文摘Status of Studying Laser Ion Source￥ZhaoZhizheng;YangYongfeng;ZhuShaofei;CaiXichen;ShangRenchengandXuSida(PhysicsDepartment,T... | Zhao Zhizheng Yang Yongfeng Zhu Shaofei Cai Xichen Shang Rencheng and Xu Sida(Physics Department,Tsinghua University.) | 1994 | IMP & HIRFL Annual Report1994，，0： | 0 |
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