|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 2 | Effects of losartan on expression of connexins at the early stage of atherosclerosis in rabbits显示文摘 | Ruan LM Cai W Chen JZ | | 0，，02： | 1 |
| 3 | Health,life expectancy,and health care spending among the elderly显示文摘 | Lubitz L Cai LM Cramarow E | | 0，，11： | 1 |
| 4 | Comparison of toxicity effects of ropivacaine,bupivaeaine, and lidoeaine on rabbit inter- vertebral disc cells in vitro 显示文摘 | CAI XY XIONG LM YANG SH | 2014 | Spine J2014，14，3： | 1 |
| 5 | Epstein-Barr virus-encoded microRNA BART1 induces tumour metastasis by regulating PTEN-dependent pathways in nasopharyngeal carcinoma 显示文摘 | Cai LM Ye YF Jiang Q | 2015 | Nat Commun2015，6，： | 1 |
| 6 | Effects of Losartan on expression of connexins at the early stage of atherosclerosis in rabbits显示文摘 | Ruan LM Cai W Chen JZ | 2010 | Int J Med Sci2010，7，2： | 1 |
| 7 | EBV-miR-BART7-3p promotes the EMT and metastasis of nasopharyngeal eareinoma cells by suppressing the tumor suppressor PTEN 显示文摘 | CAI LM LYU XM LUO WR | 2015 | Oncogene2015，34，17： | 1 |
| 8 | Semisynthesis of an antifungal lipopeptide echinoeandin显示文摘 | Journet M Cai DW DiMichele LM | 1999 | J Org Chem1999，64，7： | 1 |
| 9 | Endoscopic mucosal resection vs endoscopic submucosal dissection for rectal carcinoid tumours: a systematic review and meta-analysis显示文摘 | Zhong DD Shao LM Cai JT | 2013 | Colorectal Dis2013，15，3： | 1 |
| 10 | Endoscopic mucosal resection vs endoscopic submucosal dissection for rectal carcinoid tumours:a systematic review and meta analysis显示文摘 | Zhong DD Shao LM Cai JT | 2013 | Colorectal Dis2013，15，28： | 1 |
| 11 | Dynamics of a nonautonomous predator-prey dispersion-delay system with BeddingDeAngelis functional response显示文摘 | Cai LM Li XZ Yu JY | 2009 | Chaos solitons fractals2009，40，： | 1 |
| 12 | Description of a new species of genus Epidaus Sta1 (Hemiptera: Reduviidae:Harpactorinae) from China, with a key to Chinese species 显示文摘 | ZHANG W J ZHAO P CAO LM CAI WZ | 2010 | Zootaxa2010，，2517： | 1 |
| 13 | Improving effects of huperzine A on spatial working memory in aged monkeys and young adult monkeys with experimental cognitive impairment 显示文摘 | Ye JW Cai JX Wang LM | 1999 | J Pharmacol Exp Ther1999，288，2： | 1 |
| 14 | Efficient renal clearance of DNA tetrahedron nanopartides enables quantitative evaluation of kidney function显示文摘DNA tetrahedro n nano structure (DTN) is one of the simplest DNA nano structures and has bee n successfully applied for biose nsin g, imagi ng, and treatment of can cer. To facilitate its biomedical applications and pote ntial clinical tran slation, fun dame ntal un derstandi ng of DTN's transportation among major organs in living organisms becomes increasingly important. Here, we describe the efficient renal clearanee of DTN in healthy mice by using positron emission tomography (PET) imaging. The kidney elimination of DTN was later applied for renal function evaluation in murine models of unilateral ureteral obstruction (UUO). We further established a mathematical program of DTN to validate its changes of transportation pattern in healthy and UUO mice. We believe the establishment of pharmacokinetic profiles and mathematical model of DTN may provide in sight for future optimization of DNA nano structures for biomedical applications. | Dawei Jiang Hyung-Jun lm Madeline E. Boleyn Christopher G. England Dalong Ni Lei Kang Jonathan W. Engle Peng Huang Xiaoli Lan Weibo Cai | 2019 | Nano Research2019，12，3： | 1 |
| 15 | Highly sensitive colorimetric sensor for Hg2+ detection based on cationic polymer/DNA interaction 显示文摘 | Zhu YY Cai YL Zhu YB Zheng LX Ding JY Quan Y Wang LM Qi B | 2015 | Biosens Bioelectron2015，69，： | 1 |
| 16 | Improving effects of Huperzine A on spatial working memory in aged monkeys and young adult monkeys with expeirmental cognitive impairment 显示文摘 | Ye JW Cai JX Wang LM | 1999 | Pharmacol Exp Ther1999，288，： | 1 |
| 17 | Endoscopic mucosal resection (EMR) versus endoscopic submucosal dissection (ESD) for rectal carcinoid tumours:a systematic review and meta-analysis显示文摘 | Zhong DD Shao LM Cai JT | | 0，，03： | 1 |
| 18 | Oxidation of indole by cytochrome P450 enzymes显示文摘 | Gillam EMJ Notley LM Cai H | 2000 | Biochemistry2000，39，13： | 1 |
| 19 | Improving effects of huperzine a on spatial working memory in aged monkeys and young adult monkeys with experimental cognitive impairment显示文摘 | Ye JW Cai JX Wang LM | 1999 | J Pharmacol Exp Ther1999，288，： | 1 |
| 20 | Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus 显示文摘 | Liao HX Lynch R Zhou T Gao F Alam SM Boyd SD Fire AZ Roskin KM Schramm CA Zhang Z ZhuJ Sha- piro L NISC Comparative Sequencing Program Mullikin JC Gnanakaran S Hraber P Wiehe K Kelsoe G Yang G Xia SM Montefiori DC Parks R Lloyd KE Scearce RM Soderberg KA Cohen M Kamanga G Louder MK Tran LM Chen Y Cai F Chen S Moquin S Du X Joyce MG Srivatsan S Zhang B Zheng A Shaw GM Hahn BH Kepler TB Korber BT Kwong PD Mascola JR Haynes BF | 2013 | Nature2013，496，7446： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员