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| 1 | Improvement of wheat drought and salt tolerance by expression of a stress- inducible transcription factorGmDREB of soybean (Glycinemax)显示文摘Under stress conditions such as droughthigh-salinity and low-temperature, the transcription factorof DREB (dehydration responsive element binding proteins)improved efficiently stress resistance by regulating the ex-pression of its downstream genes with various environmentastress resistance in plants. GmDREB gene (GenBank Acces-sion No. AF514908) encoding a stress-inducible transcriptionfactor was cloned by screening a cDNA library of Glycinemax cv. Jinong 27 with yeast one-hybrid method. GmDREBgene was 910 bp in length and encoded 174 amino acids con-taining a conserved AP2/EREBP DNA-binding domain of 58amino acids. Two conserved functional amino acids, valineand glutamic acid, were located on the 14th and the 19thamino acid residues in the conserved structural domain. Analkaline amino acid region (KKR) related to a nuclear local-ization signal was at the N-terminal, while an acidic aminoacid region (DDD) related to trans-activation was at theC-terminal. Plant expression vectors were constructed andtransformed into wheat by bombardment. In total, 13 trans-genic plants with Ubi::GmDREB and 11 transgenic plantswith rd29A::GmDREB were identified from 103 regenerationplants by molecular analysis. The drought and salt tolerancesof T1 transgenic lines with Ubi::GmDREB orrd29A::GmDREB were demonstrated to be improved ascompared to wild type. The result also suggested that bothUbiquitin and rd29A promoters could effectively drive theexpression of the GmDREB gene and enhance drought andsalt tolerance of T1 plants. | GAO Shiqing XU Huijun CHENG Xianguo CHEN Ming XU Zhaoshi LI Liancheng YE Xingguo DU Lipu HAO Xiaoyan MA Youzhi | 2005 | Chinese Science Bulletin2005，50，23： | 30 |
| 2 | Neural network-based matrix effect correction in EDXRF analysis显示文摘In this paper we discuss neural network-based matrix effect correction in energy dispersive X-ray fluorescence (EDXRF) analysis,with detailed algorithm to classify the samples.The method can correct the matrix effect effectively through classifying the samples automatically,and influence of X-ray absorption and enhancement by major elements of the samples is reduced.Experiments for the complex matrix effect correction in EDXRF analysis of samples in Pangang showed improved accuracy of the elemental analysis result. | TUO Xianguo CHENG Bo MU Keliang LI Zhe | 2008 | Nuclear Science and Techniques2008，19，5： | 4 |
| 3 | MC simulation of a PGNAA system for on-line cement analysis显示文摘A prompt gamma neutron activation analysis system with a 252Cf neutron source for on-line cement analysis has been simulated with the MCNP code.The results indicate that the optimum arrangement is a Bi shield of 20-mm thickness,a polyethylene moderator of 50-mm thickness,a source-to-sample distance of 70 mm,and cement samples of 1200 mm×600 mm×170 mm.To absorb thermal neutrons and suppress low-energy γ-rays,the optimum-sized sheets are 150 mm×7 mm Cd,and 150 mm×15 mm Pb. | YANG Jianbo TUO Xianguo LI Zhe MU Keliang CHENG Yi MOU Yunfeng | 2010 | Nuclear Science and Techniques2010，21，4： | 4 |
| 4 | Monte-Carlo simulation of cement neutron field distribution characteristics in PGNAA显示文摘The distribution characteristics of the neutron field in cement was simulated using the MCNP code to comply with the requirements of an online Prompt Gamma Neutron Activation Analysis system.Simulation results showed that the neutron relative flux proportion reduced with increasing cement thickness.When the cement thickness remains unchanged,the reduced proportion of thermal neutrons increases to a small extent,but the epithermal, intermediate,and fast neutrons will decrease according to the geometric progression.H element in the cement mainly affects the reduction of fast neutrons and other single-substance elements,e.g.,O,Ca,56Fe,Si,and Al.It also slows down the reduction of the fast neutrons via inelastic scattering.O contributes more than other elements in the reduction of fast neutrons.Changing the H content affects the thermal,epithermal,intermediate,and fast neutrons, while changing the Ca,Fe,and Si contents only influences the thermal,epithermal,and intermediate neutrons;hence, there is little effect on the reduction of fast neutrons. | YANG Jianbo YANG Yigang LI Yuanjing TUO Xianguo LI Zhe LIU Mingzhe CHENG Yi MU Keliang WANG Lei | 2012 | Nuclear Science and Techniques2012，23，6： | 3 |
| 5 | A Genetic-Algorithm-based Neural Network Approach for Radioactive Activity Prediction显示文摘In this paper,a genetic-algorithm-based artificial neural network(GAANN)model radioactivity prediction is proposed,which is verified by measuring results from Long Range Alpha Detector(LRAD).GAANN can integrate capabilities of approximation of Artificial Neural Networks(ANN)and of global optimization of Genetic Algorithms(GA)so that the hybrid model can enhance capability of generalization and prediction accuracy,theoretically.With this model,both the number of hidden nodes and connection weights matrix in ANN are optimized using genetic operation.The real data sets are applied to the introduced method and the results are discussed and compared with the traditional Back Propagation(BP)neural network,showing the feasibility and validity of the proposed approach. | WANG Lei TUO Xianguo YAN Yucheng LIU Mingzhe CHENG Yi LI Pingchuan | 2013 | Nuclear Science and Techniques2013，24，6： | 2 |
| 6 | Mutagenesis reveals that the rice OsMPT3 gene is an important osmotic regulatory factor显示文摘Plant mitochondrial phosphate transporters regulate phosphate transport and ATP synthesis. Determining whether they function in abiotic stress response process would shed light on their response to salt stress. We used the CRISPR/Cas9 gene-editing system to mutagenize two mitochondrial phosphate transporters, OsMPT3;1 and OsMPT3;2, to investigate their regulatory roles under salt stress. Two cas9(CRISPR-associated protein9)-free homozygous mutants, mpt33 and mpt30, were confirmed to be stable. Both OsMPT3;1 and OsMPT3;2 were markedly induced by salt stress, and their mutagenesis strongly inhibited growth and development, especially under salt stress. Mutagenesis sharply reduced the accumulation of ATP, phosphate, calcium, soluble sugar, and proline and increased osmotic potential, malondialdehyde, and Na^+ /K^+ ratio under salt stress. Both mutants demonstrate normal growth and development in the presence of ATP, revealing high sensitivity to exogenous ATP under salt stress. The mutants showed lowered rates of Na^+ efflux but also of K^+ and Ca^(2+) influx under salt stress. Mutagenesis of OsMPT3;2 altered the enrichment profiles of differentially expressed genes involved mainly in synthesis of secondary metabolites, metabolism of glycolysis, pyruvate, tricarboxylic acid cycle, in response to salt stress. The mutant displayed significant accumulation differences in 14 metabolites involved in 17 metabolic pathways, and strongly up-regulated the accumulation of glutamine, a precursor in proline synthesis, under salt stress. These findings suggest that the OsMPT3 gene modulates phosphate transport and energy supply for ATP synthesis and triggers changes in accumulation of ions and metabolites participating in osmotic regulation in rice under salt stress, thus increasing rice salt tolerance. This study demonstrates the effective application of CRISPR/Cas9 gene-editing to the investigation of plant functional genes. | Shengcai Huang Shichao Xin Guoqiang Xie Jiao Han Zhonglai Liu Bing Wang Shuqing Zhang Qingyu Wu Xianguo Cheng | 2020 | The Crop Journal2020，8，3： | 2 |
| 7 | mTOR regulate EMT through RhoA and Rac1 pathway in prostate cancer显示文摘 | XianGuo Chen HaiYan Cheng TengFei Pan Yi Liu Yang Su CuiPing Ren DaKe Huang XiaoJun Zha ChaoZhao Liang | 2015 | Mol. Carcinog2015，，10： | 1 |
| 8 | Re- search of online automatic titanium grade analyzer and method based on energy dispersive X-ray flu- orescence technology显示文摘 | TUO Xianguo LI Zhe CHENG Yi | 2010 | Applied Radiation and Isotopes2010，68，： | 1 |
| 9 | Research of online automatic titanium grade analyzer and method based on energy dispersive X-ray fluorescence technology 显示文摘 | TUO Xianguo LI Zhe CHENG Yi | 2010 | Applied Radiation and Isotopes2010，68，： | 1 |
| 10 | Cutting forces prediction in generalized pocket machining显示文摘 | Zhao cheng Wei M J W Xianguo Han | | Int J Adv Manuf Technol0，，： | 1 |
| 11 | GmDREB2,a soybean DRE-binding transcription factor,conferred drought and high-salt tolerance in transgenic plants显示文摘 | Chen Ming Wang Qiaoyan Cheng Xianguo | | 0，，02： | 1 |
| 12 | Measuring energy loss of alpha particles in different vacuum conditions显示文摘In this paper,energy loss of alpha particles in different vacuum levels is studied experimentally and via theoretical analysis.A better understanding of energy loss of a particle in vacuum will help detect more exact numbers of alpha particles.Two ^(239)Pu sources are used to measure the energy loss of a particle crossing different vacuum levels (different air pressures).The experimental data are obtained from an instrument-PAM-100 developed by authors. The experimental results have shown that increasing vacuum levels will lead to more alpha residual energy but less energy loss.When the vacuum level reaches 0.04 MPa,alpha particles(^(239)Pu,5.115 MeV)will lose the energy of about 0.175 MeV with traversing 5 mm distance.Theoretical calculations have shown a good agreement with experimental results.This implies that the instrument has a high accuracy and could be applied in field work. | CHENG Yi TUO Xianguo HUANG Lianmei LI Zhe YANG Jianbo ZHOU Chuanwen SONG Qianqian | 2011 | Nuclear Science and Techniques2011，22，3： | 0 |
| 13 | Impacts of Climate and Nutrients on Carbon Sequestration Rate by Wetlands: A Meta-analysis显示文摘Global numerous wetlands are the most productive ecosystem and have high carbon sequestration potential to mitigate increasing CO2 in the atmosphere. However, few are available on estimating average carbon sequestration rates by global wetlands(Carbonsq) at century timescale. In this article, Carbonsq data of 473 wetland soil/sediment cores from the literatures were collected in detail by the meta-analysis method. These cores were no more than 300 years old and spanned a latitudinal range from 33.6° S to 69.7° N. Globally, the average Carbonsq was 185.2 g/(m^2·yr) regardless of wetland types. Carbonsq varied remarkably between wetland types and ranked as an order of salt marsh(247.7 g/(m^2·yr)) > mangrove(229.8 g/(m^2·yr)) > freshwater marsh(196.7 g/(m^2·yr)) > peatland(76.9 g/(m^2·yr)). Carbonsq was positively related to mean annual temperature(AMT) and annual precipitation(Pre). Nitrogen was the most common and primary factor controlling Carbonsq regardless of wetland types. | CHENG Caifeng LI Min XUE Zhenshan ZHANG Zongsheng LYU Xianguo JIANG Ming ZHANG Hongri | 2020 | Chinese Geographical Science2020，30，3： | 0 |
| 14 | A cluster of mutagenesis revealed an osmotic regulatory role of the OsPIP1 genes in enhancing rice salt tolerance显示文摘Aquaporins play important regulatory roles in improving plant abiotic stress tolerance.To better understand whether the Os PIP1 genes collectively dominate the osmotic regulation in rice under salt stress,a cluster editing of the Os PIP1;1,Os PIP1;2 and Os PIP1;3 genes in rice was performed by CRISPR/Cas9 system.Sequencing showed that two mutants with Cas9-free,line 14 and line 18 were successfully edited.Briefly,line 14 deleted a single C base in both the Os PIP1;1 and Os PIP1;3 genes,and inserted a single T base in the Os PIP1;2 gene,respectively.While line 18 demonstrated an insertion of a single A base in the Os PIP1;1gene and a single T base in both the Os PIP1;2 and Os PIP1;3 genes,respectively.Multiplex editing of the Os PIP1 genes significantly inhibited photosynthetic rate and accumulation of compatible metabolites,but increased MDA contents and osmotic potentials in the mutants,thus delaying rice growth under salt stress.Functional loss of the Os PIP1 genes obviously suppressed the expressions of the Os PIP1,Os SOS1,Os CIPK24 and Os CBL4 genes,and increased the influxes of Na+and effluxes of K^(+)/H^(+)in the roots,thus accumulating more Na+in rice mutants under salt stress.This study suggests that the Os PIP1 genes are essential modulators collectively contributing to the enhancement of rice salt stress tolerance,and multiplex editing of the Os PIP1 genes provides insight into the osmotic regulation of the PIP genes. | Leyuan Tao Bing Wang Shichao Xin Wei Li Shengcai Huang Laihua Liu Jing Cui Qianru Zhang Xianguo Cheng | 2023 | The Crop Journal2023，11，4： | 0 |
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