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| 1 | Promoter hypermethylation of CDH1, FHIT, MTAP and PLAGL1 in gastric adenocarcinoma in individuals from Northern Brazil显示文摘AIM: To evaluate the methylation status of CDH1, FHIT, MTAP and PLAGL1 promoters and the association of these findings with clinico-pathological characteristics. METHODS: Methylation-specific PCR (MSP) assay was performed in 13 nonneoplastic gastric adenocarcinoma, 30 intestinal-type gastric adenocarcinoma and 35 diffuse- type gastric adenocarcinoma samples from individuals in Northern Brazil. Statistical analyses were performed using the chi-square or Fisher’s exact test to assess associations between methylation status and clinico- pathological characteristics. RESULTS: Hypermethylation frequencies of CDH1, FHIT, MTAP and PLAGL1 promoter were 98.7%, 53.9%, 23.1% and 29.5%, respectively. Hypermethylation of three or four genes revealed a significant association with diffuse-type gastric cancer compared with nonneoplastic cancer. A higher hypermethylation frequency wassignificantly associated with H pylori infection in gastric cancers, especially with diffuse-type. Cancer samples without lymph node metastasis showed a higher FHIT hypermethylation frequency. MTAP hypermethylation was associated with H pylori in gastric cancer samples, as well as with diffuse-type compared with intestinal-type. In diffuse-type, MTAP hypermethylation was associated with female gender. CONCLUSION: Our findings show differential gene methylation in tumoral tissue, which allows us to conclude that hypermethylation is associated with gastric carcinogenesis. MTAP promoter hypermethylation can be characterized as a marker of diffuse-type gastric cancer, especially in women and may help in diagnosis, prognosis and therapies. The H pylori infectious agent was present in 44.9% of the samples. This infection may be correlated with the carcinogenic process through the gene promoter hypermethylation, especially the MTAP promoter in diffuse-type. A higher H pylori infection in diffuse-type may be due to greater genetic predisposition. | Mariana Ferreira Leal Eleonidas Moura Lima Patrícia Natália Oliveira Silva Paulo Pimentel Assumpo Danielle Queiroz Calcagno Spencer Luiz Marques Payo Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith | 2007 | World Journal of Gastroenterology2007，13，18： | 24 |
| 2 | Role of mi RNAs and their potential to be useful as diagnostic and prognostic biomarkers in gastric cancer显示文摘Alterations in epigenetic control of gene expression play an important role in many diseases, including gastric cancer. Many studies have identified a large number of upregulated oncogenic mi RNAs and downregulated tumour-suppressor mi RNAs in this type of cancer. In this review, we provide an overview of the role of mi RNAs, pointing to their potential to be useful as diagnostic and/or prognostic biomarkers in gastric cancer. Moreover, we discuss the influence of polymorphisms and epigenetic modifications on mi RNA activity. | Kelly Cristina da Silva Oliveira Taíssa Maíra Thomaz Araújo Camila Inagaki Albuquerque Gabriela Alcantara Barata Carolina Oliveira Gigek Mariana Ferreira Leal Fernanda Wisnieski Fernando Augusto Rodrigues Mello Junior André Salim Khayat Paulo Pimentel de Assumpcao Rommel Mário Rodriguez Burbano Marília Cardoso Smith Danielle Queiroz Calcagno | 2016 | World Journal of Gastroenterology2016，22，35： | 11 |
| 3 | Interrelationship between chromosome 8 aneuploidy,C-MYC amplification and increased expression in individuals from northern Brazil with gastric adenocarcinoma显示文摘AIM: To investigate chromosome 8 numerical aberra- tions, C-MYC oncogene alterations and its expression in gastric cancer and to correlate these findings with histo- pathological characteristics of gastric tumors. METHODS: Specimens were collected surgically from seven patients with gastric adenocarcinomas. Immu- nostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) for C-MYC gene and chromosome 8 centromere were performed. RESULTS: All the cases showed chromosome 8 aneu- ploidy and C-MYC amplification, in both the diffuse and intestinal histopathological types of Lauren. No significant difference (P < 0.05) was observed between the level ofchromosome 8 ploidy and the site, stage or histological type of the adenocarcinomas. C-MYC high amplification, like homogeneously stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Structural rearrangement of C-MYC, like translocation, was observed only in the diffuse type. Regarding C-MYC gene, a significant difference (P < 0.05) was observed between the two histological types. The C-MYC protein was expressed in all the studied cases. In the intestinal- type the C-MYC immunoreactivity was localized only in the nucleus and in the diffuse type in the nucleus and cytoplasm. CONCLUSION: Distinct patterns of alterations between intestinal and diffuse types of gastric tumors support the hypothesis that these types follow different genetic path- ways. | Danielle Queiroz Calcagno Mariana Ferreira Leal Aline Damaceno Seabra Andre Salim Khayat Elizabeth Suchi Chen Samia Demachki Paulo Pimentel Assumpcao Mario Henrique Girao Faria Silvia Helena Barem Rabenhorst Márcia Valéria Pitombeira Ferreira Marília de Arruda Cardoso Smith Rommel Rodríguez Burbano | 2006 | World Journal of Gastroenterology2006，12，38： | 9 |
| 4 | MYC and gastric adenocarcinoma carcinogenesis显示文摘MYC is an oncogene involved in cell cycle regulation,cell growth arrest,cell adhesion,metabolism,ribosome biogenesis,protein synthesis,and mitochondrial function. It has been described as a key element of several carcinogenesis processes in humans. Many studies have shown an association between MYC deregulation and gastric cancer. MYC deregulation is also seen in gastric preneoplastic lesions and thus it may have a role in early gastric carcinogenesis. Several studies have suggested that amplification is the main mechanism of MYC deregulation in gastric cancer. In the present review,we focus on the deregulation of the MYC oncogene in gastric adenocarcinoma carcinogenesis,including its association with Helicobacter pylori (H pylori) and clinical applications. | Danielle Queiroz Calcagno Mariana Ferreira Leal Paulo Pimentel Assumpo Marília de Arruda Cardoso Smith Rommel Rodríguez Burbano | 2008 | World Journal of Gastroenterology2008，14，39： | 8 |
| 5 | Clinical implication of 14-3-3 epsilon expression in gastric cancer显示文摘AIM:To evaluate for the first time the protein and mRNA expression of 14-3-3εin gastric carcinogenesis.METHODS:14-3-3εprotein expression was determined by western blotting,and mRNA expression was examined by real-time quantitative RT-PCR in gastric tumors and their matched non-neoplastic gastric tissue samples.RESULTS:Authors observed a significant reduction of 14-3-3εprotein expression in gastric cancer(GC)samples compared to their matched non-neoplastic tissue.Reduced levels of 14-3-3εwere also associated with diffuse-type GC and early-onset of this pathology.Our data suggest that reduced 14-3-3εmay have a role in gastric carcinogenesis process.CONCLUSION:Our results reveal that the reduced 14-3-3εexpression in GC and investigation of 14-3-3ε interaction partners may help to elucidate the carcino-genesis process. | Mariana Ferreira Leal Danielle Queiroz Calcagno Smia Demachki Paulo Pimentel Assumpo Roger Chammas Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith | 2012 | World Journal of Gastroenterology2012，18，13： | 6 |
| 6 | Association between Helicobacterpylori, Epstein-Barr virus, human papillomavirus and gastric adenocarcinomas显示文摘AIM To correlate Helicobacter pylori(H. pylori), Epstein-Barr virus(EBV) and human papillomavirus(HPV) with gastric cancer(GC) cases in Pará State, Brazil.METHODS Tissue samples were obtained from 302 gastric adenocarcinomas. A rapid urease test was used to detect the presence of H. pylori, and the presence of the cagA gene in the HP-positive samples was confirmed by PCR. An RNA in situ hybridization test designed to complement Eber1 RNA was used to detect the presence of EBV in the samples, and the L1 region of HPV was detected using nested PCR. Positive HPV samples were genotyped and analyzed for E6 and E7 viral gene expression. Infections were also correlated with the clinical and pathological characteristics of the patients.RESULTS The majority of the 302 samples analyzed were obtained from men(65%) aged 55 years or older(67%) and were classified as the intestinal subtype(55%). All three pathogens were found in the samples analyzed in the present study(H. pylori : 87%, EBV: 20%, HPV: 3%). Overall, 78% of the H. pylori-positive(H. pylori+) samples were cag A +(H. pylori-cag A+), and there was an association between the cytotoxic product of this gene and EBV. Coinfections of H. pylori-cagA+ and EBV were correlated with the most advanced tumor stages. Although only 20% of the tumors were positive for EBV, infection with this virus was associated with distant metastasis. Only the HPV 16 and 18 strains were found in the samples, although no expression of the E6 and E7 oncoproteins was detected. The fundus of the stomach was the region least affected by the pathogens.CONCLUSION HPV was not involved in gastric tumorigenesis. Prophylactic and therapeutic measures against H. pylori and EBV may prevent the development of GC, especially the more aggressive forms. | Carolina Rosal Teixeira de Souza Marcelli Carolini Alves Almeida Andre Salim Khayat Emerson Lucena da Silva Paulo Cardoso Soares Luiz Claudio Chaves Rommel Mario Rodriguez Burbano | 2018 | World Journal of Gastroenterology2018，24，43： | 6 |
| 7 | Impacts of land use and land cover changes on hydrological processes and sediment yield determined using the SWAT model显示文摘Land use and land cover(LULC)changes strongly affect local hydrology and sediment yields.The current study focused on a basin in the Brazilian Amazon and had the following three objectives:(1)to perform an effective diagnosis of flow and sediment yield,(2)to evaluate the impacts of LULC changes over the last 40 years on the hydro-sedimentological variables,and(3)to investigate the impacts of the possible trends or breaking points in the flow,surface runoff,and sediment yield series.The Soil and Water Assessment Tool(SWAT)model validation showed that the simulated results were consistent with the data measured in the dynamic reproduction of flow seasonality.Furthermore,changes in LULC altered surface runoff,sediment yield,and flow according to the Mann-Kendall and Pettitt non-parametric tests.It was also observed that the sub-basins in which pastureland is predominant are more susceptible to increased surface runoff and sediment yield.According to trend and homogeneity tests,these sub-basins had break points in the time series of these variables.On the other hand,in the sub-basins whose land cover is predominantly forest,the time series is homogeneous and trendless.The current study highlighted the impacts that 40 years of LULC changes in an Amazonian basin had on hydro-sedimentological variables,which,in turn,can play an important role in changing the hydrological cycle,consequently these changes can also impact diverse human activities in the region,such as agribusiness,livestock,energy production,food security,and public water supply.Thus,it is concluded that the SWAT model is a good estimator of hydro-sedimentological processes in Amazonian basins and can be used by decision makers in the management of water and environmental resources. | Edivaldo Afonso de Oliveira Serrao Madson Tavares Silva Thomas Rocha Ferreira Lorena Conceiçao Paiva de Ataide Cleber Assis dos Santos Aline Maria Meiguins de Lima Vicente de Paulo Rodrigues da Silva Francisco de Assis Salviano de Sousa Denis Jose Cardoso Gomes | 2022 | International Journal of Sediment Research2022，37，1： | 3 |
| 8 | Parallel computing techniques applied to the simultaneous design of structure and material显示文摘 | Coelho Pedro G Cardoso Joao B Fernandes Paulo R | 2011 | Adv Eng Software2011，42，5： | 1 |
| 9 | Synthesis and characterization of novel diazenes bearing pyrrole, thiophene and thiazole heterocycles as efficient photochromic and nonlinear optical (NLO) materials显示文摘 | M. Manuela M. Raposo A. Maurício C. Fonseca M. Cidália R. Castro M. Belsley M. Fátima S. Cardoso Luís M. Carvalho Paulo J. Coelho | 2011 | Dyes and Pigments2011，，1： | 1 |
| 10 | Characterization of azo reduction activity in a novel asco- mycete yeast strain显示文摘 | RAMALHO P A CARDOSO M H CAVACO PAULO A | 2004 | Applied and Environmental Microbiol- ogy2004，70，4： | 1 |
| 11 | Trisomy 18: review of the clinical,etiologic,prognostic,and ethical aspects显示文摘 | Rafael Fabiano M Rosana Cardoso M Paulo Ricardo G | 2013 | Rev Paul Pediatr2013，31，1： | 1 |
| 12 | On the sonication of chitin:effects on its structure and morphology and influence on its deacetylation显示文摘 | Márcia Barreto Cardoso Roberta Signini Sérgio Paulo Campana-Filho | 2001 | Polymer Bulletin2001，47，2： | 1 |
| 13 | Effect of the reinforcement ( carbon or glass fibres ) on friction and wear behaviour of the PEEK against steel surface at a long dry sliding 显示文摘 | Paulo Davim J Cardoso Rosaria | 2009 | Wear2009，266，78： | 1 |
| 14 | The correlation between age, body weight and testicular parameters in Murrah Buffalo bulls raised in Brazil显示文摘 | Luz Patricia Aparecida Cardoso da Santos Paulo Ramos da Silva Andrighetto Cristiana | 2012 | Journal of Reproduction and Devel- opment2012，59，1： | 1 |
| 15 | New approach for obtaining the C-factor of RUSLE considering the seasonal effect of rainfalls on vegetation cover显示文摘We present a new approach for calculating the C-factor of RUSLE considering the effect of low-reflectance vegetation cover areas on the reduction of the effects on erosion caused by rainfall seasonality.For this,we propose the coefficients Cr2(rescaled 2)and C-PC(Precipitation Correction),which represent the Cfactor,and an adaptation in NDVI calculation,according to the seasonality of precipitation(NDVI-PC).The Cr2 factor is used when there is no seasonal effect of rainfall on vegetation,while the C-PC factor is calculated for localities under the influence of seasonality,from NDVI-PC.The proposed approaches were tested using different satellites images in the Palmares-Ribeir~ao do Saco watershed,Rio de Janeiro,Brazil.The values of Cr2 and C-PC factors were compared to the Cr factor(rescaled)and to mean values from the literature for different land covers.Our results indicated that the Cr2 factor represents an improvement in accuracy in relation to Cr by considering specific values of the studied area to normalize the data without generalizations.Furthermore,the C-PC factor is able to simulate the effect of seasonality,providing more realistics values of soil loss by the RUSLE as a function of the proportion of area affected by the rainfall seasonality obtained from NDVI-PC.We conclude that both Cr2 and C-PC factors generate values similar of the C-factor observed in the literature,and therefore are able to provide better soil loss estimation than that using the Cr factor. | Pietro Menezes Sanchez Macedo Paulo Tarso Sanches Oliveira Mauro Antonio Homem Antunes Valdemir Lucio Durigon Elaine Cristina Cardoso Fidalgo Daniel Fonseca de Carvalho | 2021 | International Soil and Water Conservation Research2021，9，2： | 1 |
| 16 | hTERT methylation and expression in gastric cancer显示文摘 | Carolina Oliveira Gigek Mariana Ferreira Leal Patricia Natalia Oliveira Silva Luara Carolina Frias Lisboa Eleonidas Moura Lima Danielle Queiroz Calcagno Paulo Pimentel Assump??o Rommel Rodriguez Burbano Marilia de Arruda Cardoso Smith | 2009 | Biomarkers2009，，8： | 1 |
| 17 | Yarn hairiness parameterization using a coherent signal processing technique 显示文摘 | CARVALHO Vitor CARDOSO Paulo BELSLEY Michael | 2008 | Sensors and Actuators2008，142，： | 1 |
| 18 | Yarn hairiness and diameter characterization using a CMOS line array 显示文摘 | CARVALHO Vitor CARDOSO Paulo VASCONCELOS Rosa | 2008 | Measurement2008，41，： | 1 |
| 19 | Metastatic cancer to the stomach显示文摘 | Paulo Moacir de Oliveira Campoli Flávio Hayato Ejima Daniela Medeiros Milhomem Cardoso Osterno Queiroz da Silva Jales Benevides Santana Filho Paulo Adriano de Queiroz Barreto Márcio Martins Machado Eliane Duarte Mota Jo?o Alves Araujo Filho Rita de Cássia | 2006 | Gastric Cancer2006，，1： | 1 |
| 20 | Hedonic and utilitarian shopping motivations among Portuguese young adult consumers显示文摘 | Paulo Ribeiro Cardoso Sara Carvalho Pinto | 2010 | International Journal of Retail & Distribution Management2010，，7： | 1 |
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