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| 1 | TiO_2 nanotubes as solid-phase extraction adsorbent for the determination of polycyclic aromatic hydrocarbons in environmental water samples显示文摘An analytical method based on TiO 2 nanotubes solid-phase extraction (SPE) combined with gas chromatography (GC) was established for the analysis of seven polycyclic aromatic hydrocarbons (PAHs): acenaphtylene, acenaphthene, anthracene, fluorene, phenanthrene, fluoranthene and pyrene. Factors affecting the extraction efficiency including the eluent type and its volume, adsorbent amount, sample volume, sample pH and sample flow rate were optimized. The characteristic data of analytical performance were determined to investigate the sensitivity and precision of the method. Under the optimized extraction conditions, the method showed good linearity in the range of 0.01-0.8 μg/mL, repeatability of the extraction (RSD were between 6.7% and 13.5%, n = 5) and satisfactory detection limits (0.017-0.059 ng/mL). The developed method was successfully applied to the analysis of surface water (tap, river and dam) samples. The recoveries of PAHs spiked in environmental water samples ranged from 90% to 100%. All the results indicated the potential application of titanate nanotubes as solid-phase extraction adsorbents to pre-treat water samples. | Bochra Bejaoui Kefi Latifa Latrous El Atrache Hafedh Kochkar Abdelhamid Ghorbel | 2011 | Journal of Environmental Sciences2011，23，5： | 10 |
| 2 | Role of sirtuins in ischemia-reperfusion injury显示文摘Ischemia-reperfusion injury(IRI)remains an unresolved and complicated situation in clinical practice,especially in the case of organ transplantation.Several factors contribute to its complexity;the depletion of energy during ischemia and the induction of oxidative stress during reperfusion initiate a cascade of pathways that lead to cell death and finally to severe organ injury.Recently,the sirtuin family of nicotinamide adenine dinucleotide-dependent deacetylases has gained increasing attention from researchers,due to their involvement in the modulation of a wide variety of cellular functions.There are seven mammalian sirtuins and,among them,the nuclear/cytoplasmic sirtuin 1(SIRT1)and the mitochondrial sirtuin 3(SIRT3)are ubiquitously expressed in many tissue types.Sirtuins are known to play major roles in protecting against cellular stress and in controlling metabolic pathways,which are key processes during IRI.In this review,we mainly focus on SIRT1 and SIRT3 and examine their role in modulating pathways against energy depletion during ischemia and their involvement in oxidative stress,apoptosis,microcirculatory stress and inflammation during reperfusion.We present evidence of the beneficial effects of sirtuins against IRI and emphasize the importance of developing new strategies by enhancing their action. | Eirini Pantazi Mohamed Amine Zaouali Mohamed Bejaoui Emma Folch-Puy Hassen Ben Abdennebi Joan Roselló-Catafau | 2013 | World Journal of Gastroenterology2013，19，43： | 7 |
| 3 | Effects of Institut Georges Lopez-1 and Celsior preservation solutions on liver graft injury显示文摘AIM: To compare Institut Georges Lopez(IGL-1) and Celsior preservation solutions for hepatic endothelium relaxation and liver cold ischemia reperfusion injury(IRI).METHODS: Two experimental models were used.In the first one, acetylcholine-induced endotheliumdependent relaxation(EDR) was measured in isolated ring preparations of rat hepatic arteries preserved or not in IGL-1 or Celsior solutions(24 h at 4 ℃).To determine nitric oxide(NO) and cyclooxygenase EDR, hepatic arteries were incubated with L-NG-nitroarginine methyl ester(L-NAME), an inhibitor of endothelium nitric oxide synthase(e NOS), or with L-NAME plus indomethacin, an inhibitor of cyclooxygenase.In the second experiment, rat livers were cold-stored in IGL-1 or Celsior solutions for 24 h at 4 ℃ and then perfused 'ex vivo ' for 2 h at 37 ℃.Liver injury was assessed by transaminase measurements, liver function by bile production and bromosulfophthalein clearance, oxidative stress by malondialdehyde levels and catalase activity and alterations in cell signaling pathways by pA kt, pA MPK, eN OS and MAPKs proteins level.RESULTS: After cold storage for 24 h with either Celsior or IGL-1, EDR was only slightly altered.Infreshly isolated arteries, EDR was exclusively mediated by NO.However, cold-stored arteries showed NOand COX-dependent relaxation.The decrease in NO-dependent relaxation after cold storage was significantly more marked with Celsior.The second study indicated that IGL-1 solution obtained better liver preservation and protection against IRI than Celsior.Liver injury was reduced, function was improved and there was less oxidative stress.IGL-1 solution activated Akt and AMPK, which was concomitant with increased eN OS expression and nitrite/nitrate levels.Furthermore, MAPKs kinases were regulated in livers preserved with IGL-1 solution since reductions in p-p38, p-ERK and p-JNK protein levels were observed.CONCLUSION: IGL-1 solution preserved NO-dependent relaxation better than Celsior storage solution and enhanced liver graft preservation. | Donia Tabka Mohamed Bejaoui James Javellaud Joan Roselló-Catafau Jean-Michel Achard Hassen Ben Abdennebi | 2015 | World Journal of Gastroenterology2015，21，14： | 6 |
| 4 | Polyethylene glycol rinse solution:An effective way to prevent ischemia-reperfusion injury显示文摘AIM:To test whether a new rinse solution containing polyethylene glycol 35(PEG-35)could prevent ischemia-reperfusion injury(IRI)in liver grafts.METHODS:Sprague-Dawley rat livers were stored in University of Wisconsin preservation solution and then washed with different rinse solutions(Ringer’s lactate solution and a new rinse solution enriched with PEG-35 at either 1 or 5 g/L)before ex vivo perfusion with Krebs-Heinseleit buffer solution.We assessed the following:liver injury(transaminase levels),mitochondrial damage(glutamate dehydrogenase activity),liver function(bile output and vascular resistance),oxidative stress(malondialdehyde),nitric oxide,liver autophagy(Beclin-1 and LCB3)and cytoskeleton integrity(filament and globular actin fraction);as well as levels of metalloproteinases(MMP2 and MMP9),adenosine monophosphate-activated protein kinase(AMPK),heat shock protein 70(HSP70)and heme oxygenase 1(HO-1).RESULTS:When we used the PEG-35 rinse solution,reduced hepatic injury and improved liver function were noted after reperfusion.The PEG-35 rinse solution prevented oxidative stress,mitochondrial damage,and liver autophagy.Further,it increased the expression of cytoprotective heat shock proteins such as HO-1 and HSP70,activated AMPK,and contributed to the restoration of cytoskeleton integrity after IRI.CONCLUSION:Using the rinse solution containing PEG-35 was effective for decreasing liver graft vulnerability to IRI. | Mohamed Amine Zaouali Mohamed Bejaoui Maria Calvo Emma Folch-Puy Eirini Pantazi Gianfranco Pasut Antoni Rimola Hassen Ben Abdennebi René Adam Joan Roselló-Catafau | 2014 | World Journal of Gastroenterology2014，20，43： | 6 |
| 5 | Emerging concepts in liver graft preservation显示文摘The urgent need to expand the donor pool in order to attend to the growing demand for liver transplantation has obliged physicians to consider the use of suboptimal liver grafts and also to redefine the preservation strategies. This review examines the different methods of liver graft preservation, focusing on the latest advances in both static cold storage and machine perfusion(MP). The new strategies for static cold storage are mainly designed to increase the fatty liver graft preservation via the supplementation of commercial organ preservation solutions with additives. In this paper we stress the importance of carrying out effective graft washout after static cold preservation, and present a detailed discussion of the future perspectives for dynamic graft preservation using MP at different temperatures(hypothermia at 4 ℃, normothermia a t 3 7 ℃ and subnormothermia at 20 ℃- 2 5 ℃). Finally, we highlight some emerging applications of regenerative medicine in liver graft preservation. In conclusion, this review discusses the 'state of the art' and future perspectives in static and dynamic liver graft preservation in order to improve graft viability. | Mohamed Bejaoui Eirini Pantazi Emma Folch-Puy Pedro M Baptista Agustín García-Gil René Adam Joan Roselló-Catafau | 2015 | World Journal of Gastroenterology2015，21，2： | 5 |
| 6 | Sirtuin 1 in rat orthotopic liver transplantation:An I GL-1 preservation solution approach显示文摘AIM:To investigate the possible involvement of Sirtuin1(SIRT1)in rat orthotopic liver transplantation(OLT),when Institute Georges Lopez 1(IGL-1)preservation solution is enriched with trimetazidine(TMZ).METHODS:Male Sprague-Dawley rats were used as donors and recipients.Livers were stored in IGL-1 preservation solution for 8h at 4℃,and then underwent OLT according to Kamada’s cuff technique without arterialization.In another group,livers were stored in IGL-1 preservation solution supplemented with TMZ,at10-6 mol/L,for 8 h at 4℃and then underwent OLT.Rats were sacrificed 24 h after reperfusion,and liver and plasma samples were collected.Liver injury(transaminase levels),mitochondrial damage(glutamate dehydrogenase activity)oxidative stress(malondialdehyde levels),and nicotinamide adenine dinucleotide(NAD+),the cofactor necessary for SIRT1 activity,were determined by biochemical methods.SIRT1 and its substrates(acFox O1,ac-p53),the precursor of NAD+,nicotinamide phosphoribosyltransferase(NAMPT),as well as the phosphorylation of adenosine monophosphate activated protein kinase(AMPK),p-m TOR,p-p70S6K(direct substrate of m TOR),autophagy parameters(beclin-1,LC3B)and MAP kinases(p-p38 and p-ERK)were determined by Western blot.RESULTS:Liver grafts preserved in IGL-1 solution enriched with TMZ presented reduced liver injury and mitochondrial damage compared with those preservedin IGL-1 solution alone.In addition,livers preserved in IGL-1+TMZ presented reduced levels of oxidative stress.This was consistent with enhanced SIRT1 protein expression and elevated SIRT1 activity,as indicated by decreased acetylation of p53 and Fox O1.The elevated SIRT1 activity in presence of TMZ can be attributed to the enhanced NAMPT protein and NAD+/NADH levels.Up-regulation of SIRT1 was consistent with activation of AMPK and inhibition of phosphorylation of m TOR and its direct substrate(p-p70S6K).As a consequence,autophagy mediators(beclin-1 and LC3B)were overexpressed.Furthermore,MAP kinases were regulated in livers preserved with IGL-1+TMZ,as they were characterized by enhanced p-ERK and decreased p-p38protein expression.CONCLUSION:Our study shows that IGL-1 preservation solution enriched with TMZ protects liver grafts from the IRI associated with OLT,through SIRT1 up-regulation. | Eirini Pantazi Mohamed Amine Zaouali Mohamed Bejaoui Emma Folch-Puy Hassen Ben Abdennebi Ana Teresa Varela Anabela Pinto Rolo Carlos Marques Palmeira Joan Roselló-Catafau | 2015 | World Journal of Gastroenterology2015，21，6： | 5 |
| 7 | Bortezomib enhances fatty liver preservation in I nstitut G eorge L opez‐1 solution through adenosine monophosphate activated protein kinase and Akt / mTOR pathways显示文摘 | Mohamed Bejaoui Mohamed Amine Zaouali Emma Folch‐Puy Eirini Pantazi Fawzia Bardag‐Gorce Teresa Carbonell Joan Oliva Antoni Rimola Hassen Ben Abdennebi Joan Roselló‐Catafau | 2014 | J Pharm Pharmacol2014，，1： | 3 |
| 8 | An Adverbial Approach for the Formal Specification of Topological Constraints Involving Regions with Broad Boundaries 显示文摘 | Lotfi Bejaoui | 2008 | Lecture Notes In Computer Science2008，27，6： | 1 |
| 9 | Experimental study of the E(m,2)/E(m,1064) ratio as a function of wavelength, fuel type, height above the burner and temperature显示文摘 | Bejaoui S Lemaire R Desgroux P | 2014 | Applied Physics B : Lasers and Optics2014，116，2： | 1 |
| 10 | Efficient call admission control scheme for 4G wireless networks 显示文摘 | Bejaoui T Nasser N | 2009 | Wireless Communications and Mobile Computing2009，9，： | 1 |
| 11 | Modeling of the link between microstructure and effective diffusivity of cement pastes using a simplified composite model显示文摘 | BEJAOUI S BARY B | 2004 | Applied Clay Science2004，26，： | 1 |
| 12 | Ochratoxin A removal in synthetic and natural grape juices by selected oenological Saccharomyces strains显示文摘 | Bejaoui H Mathieu F Taillandier P | 2004 | Journal of Applied Microbiology2004，97，5： | 1 |
| 13 | Optimization of the tetrazolium-based colorimetric assay for the measurement of cell number and cytotoxicity显示文摘 | Pag~ M Bejaoui N Cinq M B | 1988 | Int J Immunopharmacol1988，10，： | 1 |
| 14 | Structure of CARB-4 and AER-1 earbenieillin-hydrolyzing beta-laetamases显示文摘 | Sanschagrin F Bejaoui N Levesque RC | 1998 | Antimierob Agents Chemother1998，42，8： | 1 |
| 15 | Champignons ochratoxinogènes et ochratoxine A(OTA)dans des vignobles Franais et procédés biologiques de décontamination de l'OTA dans les mots de raisin显示文摘 | BEJAOUI H BEJAOUI H | 2005 | Bibliogr2005，，： | 1 |
| 16 | Structure of CARB-4 and AER-Ⅰ carbenicillin-hydrolyzing betalactamases显示文摘 | Sanschagrin F Bejaoui N Levesque R C | 1998 | Antimicrob Agents Chemother1998，42，8： | 1 |
| 17 | Prospective evaluation of patient - reported outcomes during treatment with deferasirox or deferoxamine for iron overload in patients with beta - thalassaemia 显示文摘 | Cappellini MD Bejaoui M Agaoglu L | 2007 | Clin Ther2007，29，5： | 1 |
| 18 | Strcture of CARB-4 and AER-1 carbenicillin-hydrolyzing beta-lactamases显示文摘 | SANSCHAGRIN F BEJAOUI N LEVESQUE R C | 1998 | Antimicrob Agents Chemother1998，42，8： | 1 |
| 19 | Clinical and genetic heterogeneity of inherited autosomal recessive susceptibility to disseminated Mycobacterium boris Bacille Calmette-Guerin infection 显示文摘 | Elloumi-Zghal H Barbouche MR Chemli J Bejaoui M Harbl A Snoussi N | 2002 | J Infect Dis2002，185，10： | 1 |
| 20 | Structure of CARB-4 and AER- 1 carbenicillin-hydrolyzing beta-lactamases显示文摘 | Sanschagrin F Bejaoui N Levesque RC | 1998 | Antimicrob Agents Chemother1998，42，8： | 1 |
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