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| 1 | Exogenous sphingomyelinase causes impaired intestinal epithelial barrier function显示文摘AIM: To test the hypothesis that hydrolysis of sphingomyelin to ceramide changes the composition of tight junctions (TJs) with increasing permeability of the intestinal epithelium. METHODS: Monolayers of Caco-2 cells were used as an in vitro model for the intestinal barrier. Permeability was determined by quantifi cation of transepithelialflux and transepithelial resistance. Sphingolipid-rich membrane microdomains were isolated by a discontinuous sucrose gradient and characterized by Western-blot. Lipid content of microdomains was analysed by tandem mass spectrometry. Ceramide was subcellularly localized by immunofluorescent staining.RESULTS: Exogenous sphingomyelinase increased transepithelial permeability and decreased transepithelial resistance at concentrations as low as 0.01 U/mL. Lipid analysis showed rapid accumulation of ceramide in the membrane fractions containing occludin and claudin-4, representing TJs. In these fractions we observed a concomitant decrease of sphingomyelin and cholesterol with increasing concentrations of ceramide. Immunofluorescent staining confirmed clustering of ceramide at the sites of cell-cell contacts. Neutralization of surface ceramide prevented the permeability-increase induced by platelet activating factor. CONCLUSION: Our findings indicate that changes in lipid composition of TJs impair epithelial barrier functions. Generation of ceramide by sphingomyelinases might contribute to disturbed barrier function seen in diseases such as inflammatory, infectious, toxic or radiogenic bowel disease. | Jürgen Bock Gerhard Liebisch Joachim Schweimer Gerd Schmitz Gerhard Rogler | 2007 | World Journal of Gastroenterology2007，13，39： | 11 |
| 2 | Highly efficient derivation of ventricular cardiomyocytes from induced pluripotent stem cells with a distinct epigenetic signature显示文摘从 pluripotent 干细胞导出的 Cardiomyocytes 能在药测试,疾病建模和基于房间的治疗被使用。没有 procardiogenic 生长因素,然而,从 pluripotent 干细胞的 cardiomyogenesis 的效率通常是低的,产生 cardiomyocyte 人口是异构的。这里,我们证明导致的 pluripotent 干细胞( iPSCs )能从鼠科的室的 myocytes ( VM )被导出,并且与从各种各样的体的房间类型导出的 iPSCs 的另外的报告一致,自发地作为与遗传上匹配的胚胎的干细胞(转换字符)或 iPSCs 相比区分 cardiomyocytes 进跳动的显著地更高的倾向从尾巴尖端成纤维细胞导出的 导出VM 的 iPSCs ( ViPSCs )展览。惊人地,导出 ViPSC 的 cardiomyocytes 显示的多数室的显型。在 ViPSCs 的提高的室的 myogenesis 在区别的早阶段经由心血管的祖先的增加的数字被调停。以便从 ViPSCs 调查提高的室的 myogenesis 的机制,我们执行了全球基因表示和 DNA methylation 分析,它揭示了可以涉及在 pluripotent 干细胞指定 VM 命运的不同 epigenetic 签名。 | Huansheng Xu B Alexander Yi Hao Wu Christoph Bock Hongcang Gu Kathy O Lui Joo-Hye C Park Ying Shao Alyssa K Riley Ibrahim J Domian Erding Hu Robert Willette John Lepore Alexander Meissner Zhong Wang Kenneth R Chien | 2012 | Cell Research2012，22，1： | 7 |
| 3 | Expression of cell volume-regulated kinase h-SGK in pancreatic tissue显示文摘 | KLINGEL K WARNTGES S BOCK J | 2000 | Am J Physiol Gastrointest Liver Physiol2000，279，5： | 1 |
| 4 | Southern California Permanent GPS Geodetic Array:Spatial Filtering of Daily Positions for Estimateing Coseismic and Postseismic Displacement Induced by the Landers Earthquake显示文摘 | WDOWINSKI S BOCK Y ZHANG J | 1997 | Journal of Geophysical Research1997，，： | 1 |
| 5 | Behavioral intention formation in knowledge sharing:Examining the roles of extrinsic motivators,social-psychological forces,and organizational chmate显示文摘 | Bock G W Zmud R W Kim Y G Lee J N | | 0，，01： | 1 |
| 6 | Bevacizumab as a potent inhibitor of inflammatory corneal angiogenesis and lymphangiogenesis 显示文摘 | Bock F Onderka J Dietrich T | 2007 | Invest Ophthalmol Vis Sci2007，48，6： | 1 |
| 7 | Organization and structure of the Methanococcus transcriptional unit homologous to the Escherichia coli 'Spectinomycin Operon' 显示文摘 | Auer J Spicker G Bock A | 1989 | J Mol Biol1989，209，1： | 1 |
| 8 | Mesodensity organo-clay associations in a near-shore sediment显示文摘 | Bock M J Mayer L M | 2000 | Marine Geology2000，163，： | 1 |
| 9 | Instantaneous Geodetic Positioning with 10-50 Hz GPS Measurements: Noise Characteristics and Implications for Monitoring Networks显示文摘 | Genrich J F Bock Y | 2006 | J Geophys Res2006，111，03： | 1 |
| 10 | Carbohydrate structuraldetermination by NMR spectroscopy : modern methods andlimitations显示文摘 | Duus J Gotfredsen C H Bock K | 2000 | Chem Rev2000，100，45： | 1 |
| 11 | Integration of endocytosis and signal transduction by lipoprotein receptors 显示文摘 | Bock HH Herz J | 2003 | Sci STKE2003，176，： | 1 |
| 12 | Tissue- and stage-specific modulation of RNA editing of the psbF and psbL transcript from spinach plastids-a new regulatory mechanism?显示文摘 | Bock R Hagemann R K?ssel H Kudla J | 1993 | Mol Gen Genet1993，240，2： | 1 |
| 13 | Sensitive and specific detection of proviral bovine leukemia virus by 5' Taq nuclease PCR using a 3' minor groove binder fluorogenic probe 显示文摘 | Lew A E Bock R E Molloy J B | 2004 | J Virol Methods2004，115，2： | 1 |
| 14 | Molecular farming for new drugs and vaccines-Current perspectives on the production of pharmaceuticals in transgenic plants显示文摘 | Ma J K C Barros E Bock R | 2005 | EMBO Rep2005，6，7： | 1 |
| 15 | Human papillomavirus-the moet significant risk determinant of cervical intraepithelial neoplasia显示文摘 | KJAER S K BRULE A J C BOCK J E | 1996 | Int J Cancer1996，65，： | 1 |
| 16 | Validation of the 4B5 rabbit monoclonal antibody in determining HER-2/neu status in breast cancer显示文摘 | van der Vegt B de Bock G H Bart J | 2009 | Mod Pathol2009，22，7： | 1 |
| 17 | Response of spring wheat to nitrogen fertilizers of different nitrification rates显示文摘 | Goos R J Schimelfenig J A Bock B R | 1999 | Agronomy Journal1999，91，2： | 1 |
| 18 | Evaluation of cardiac STATus CK-MB/myoglobin device for rapidly ruling out acute myocardial infarction显示文摘 | Brogan GX Jr Bock J L MeCuskey C F | 1997 | Clin Lab Med1997，17，： | 1 |
| 19 | Behav- ioral intention formation in knowledge sharing:Exam- ining the roles of extrinsic motivators, social-psycho- logical forces, and organizational climate 显示文摘 | Bock G W Zmud R W Kim Y G Lee J N | 2005 | MIS Quarterly2005，29，1： | 1 |
| 20 | Evaluating the use of different waveforms for intravesical electrical stimulation:a study in the rat显示文摘 | De Bock F Dirckx J Wyndaele JJ | 2011 | Neurourol Urodyn2011，30，1： | 1 |
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