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2篇 您的检索式:作者名="Ansgar Poetsch"
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1Unraveling the Acidithiobacillus caldus complete genome and its central metabolisms for carbon assimilation显示文摘Acidithiobacillus caldus 是在 bioleaching 反应堆的主导的氧化硫的细菌之一。它在在 bioleaching 过程期间维持减少的无机的硫混合物的高酸味和氧化起必要作用。在这份报告, A 的完全的染色体顺序。caldus SM-1 被介绍。染色体由一个染色体(2,932,225 bp ) 和四 plasmids 组成(pLAtc1, pLAtc2, pLAtc3, pLAtcm ) 并且它富于重复序列(为 11% 全部的染色体的财务) ,它经常与 transposable 被联系基因元素。特别地, ISAtfe 的 12 个拷贝和 ISAtc1 的 37 个拷贝被识别了,建议他们是在染色体的活跃 transposons。A。caldus SM-1 为中央新陈代谢和碳混合物, 29 蛋白质 / 酶与 proteomic 工具在之中是可看作是相同的吸收编码所有酶。SM-1 改正 CO2via 古典 CalvinBasshamBenson (CBB ) 周期,和罐头操作完全的 Embden-Meyerhof 小径(EMP ) ,戊糖磷酸盐小径(PPP ) ,和 gluconeogenesis。它有一个不完全的 tricarboxylic 酸周期(TCA ) 。涉及糖类举起的四通常认为的 transporters 被识别。一起拿,结果建议 SM-1 能吸收糖类,因为在基本的盐媒介的 1% 葡萄糖或蔗糖的增加显著地增加了 SM-1 的生长,这随后试验性地被证实。SM-1 的完全的染色体为它的生理学和遗传的进一步的调查提供了基本数据,这被结束,除了在这研究揭示的碳新陈代谢。Xiao-Yan You Xu Guo Hua-Jun Zheng Ming-Jiang Zhang Li-Jun Liu Yong-Qiang Zhu Baoli Zhu Sheng-YueWang Guo-Ping Zhao Ansgar Poetsch Cheng-Ying Jiang Shuang-Jiang Liu 2011Journal of Genetics and Genomics2011,38,6:5
2Proteomic and molecular investigations revealed that Acidithiobacillus caldus adopts multiple strategies for adaptation to NaCl stress显示文摘Acidithiobacillus caldus plays an important role in commercial bioleaching.To understand how NaCl stress adaptation occurs in A.caldus,we grew A.caldus strain SM-1 in media containing high NaCl concentrations.SM-1 grew at concentrations of up to 1.0-mol L^(-1)NaCl,but growth was severely inhibited at higher concentrations.Proteomic analysis showed that SM-1 used multiple strategies to respond to NaCl stress.In addition to several heatshock proteins,enzymes involved in proline biosynthesis increased under NaCl stress.In addition,two DNA-binding proteins and a third protein of unknown function(Atc_(1291)),which was subsequently identified as a putative single-stranded DNA-binding protein,were up-regulated in the presence of NaCl stress.These DNA-binding proteins might play a role in response to osmotic stress.Atc_(1291)was cloned and expressed in Escherichia coli.Surprisingly,we found that E.coli BL21/pET28a-atc_(1291)grew to higher cell densities than E.coli BL21/pET28a,regardless of NaCI stress.Homologs to Atc_(1291)were identified in several groups of Proleohacleria.The role of Atc_(1291)in enhancing cell growth needs further investigation.Xu Guo Chengying Jiang Yuanming Luo Mingjiang Zhang Ansgar Poetsch Shuangjiang Liu 2014Chinese Science Bulletin2014,59,3:2
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