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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Liquid Crystal Motion Picture Projector显示文摘LiquidCrystalMotionPictureProjector①SHIYongji(LuoyangTechnologyColege,Luoyang471003,CHN)Abstract:Aliquidcrystalmovingpicturep... | SHI Yongji(Luoyang Technology College,Luoyang 471003,CHN)Abstract: A liquid crystal moving picture projector and method are described.Light incident on a liquid crystal display-type device is selectively scattered or transmitted by respective portions of liquid crystal display,and a projection mechanism projects an image formed by either such scattered light or such transmitted light.A liquid crystal moving picture projector includes a liquid crystal display for creating characteristics of an image,and projecttion optics for projecting images sequentially created by the display.The display includes a liquid crystal material capable of temporary storing information at respective areas.The temporary storage may be a function of charge storing directly on liquid crystal material.A method of projecting plural images in sequence includes:creating an image or characteristics of an image in a liquid crystal material,storing such image in such liquid crystal material,directing light at such liquid crystal material,projecting such image as a function of light transmitted through or scattered by such liquid crystal material,and creating a further image in such liquid crystal material for subsequent projection. | 1997 | Semiconductor Photonics and Technology1997，3，3： | 35 |
| 3 | Effects of aminoguanidine on nitric oxide production induced by inflammatory cytokines and endotoxin in cultured rat hepatocytes显示文摘AIM To study the effects of aminoguanidine(AG) and two L-arginine analogues Nω-nitro-L-arginine methyl ester (L-NAME) and Nω-nitro-L-arginine (L-NNA) on nitric oxide (NO) productioninduced by cytokines (TNF-α, IL-11β, and IFN-γ)and bacterial lipopolysaccharide (LPS) mixture(CM) in the cultured rat hepatocytes, andexamine their mechanisms action.METHODS Rat hepatocytes were incubatedwith AG, L-NAME, L-NNA, Actinomycin D (ActD)and dexamethasene in a medium containing CM(LPS plus TNF-α, IL-1β, and IFN-γ) for 24 h. NOproduction in the cultured supernatant wasmeasured with the Griese reaction. IntracellularcGMP level was detected with radioimmunoasey.RESULTS NO production was markedlyblocked by AG and L-NAME in a dose-dependentmanner under inflammatory stimuli conditiontriggered by CM in vitro. The rate of themaximum inhibitory effects of L-NAME (38.9%)was less potent than that obtained with AG(53.7%, P<0.05). There was no significantdifference between the inhibitory effects of AGand two L-arginine analogues on intracellularcGMP accumulation in rat cultured hepatocytes.Non-specific NOS expression inhibitordexamethasone ( DEX ) and iNOS mRNAtranscriptional inhibitor ActD also significantlyinhibited CM-induced NO production. AG(0.1mmol.L-1) and ActD (0.2ng@Lt) wereequipotent in decreasing NO production inducedby inflammatory stimuli in vitro, and botheffects were more potent than that induced bynon-selectivity NOS activity inhibitor L-NAME(0. 1 mmol@ L- 1) under similar stimuli conditions(P | Guo Liang Zhang Ye Hong Wang Hui Ling Teng Zhi Bin Lin Department of Pharmacology,School of Basic Medical Sciences,Beijing University,Beijiog 100083,ChinaDr.Guo Liang Zhang graduated from Xinxiang Medical College in 1982,got Ph.D.at Nagoya City University Medical School,Japan in 1994,finished postdoctoral research at Beijing Medical Univcrsity in 1996,now an associate professor of pharmacology,specialized in hepatic pharmacology,having 15 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 20 |
| 4 | Hydrogen storage characteristics of Ti 2 Ni alloy synthesized by the electro-deoxidation technique显示文摘 | Mustafa Anik Bedri Baksan Tu?ba ?l?er Orbay Nilüfer Kü?ükdeveci Alanur Binal Aybar Re?at Can ?zden Hakan Ga?an Nur?en Ko? | 2014 | Intermetallics2014，，： | 2 |
| 5 | Exploring polymorphisms and their effects on reproductive traits of the INHA and INH:A genes in three goat breeds显示文摘 | Hou J An X Li G et at | 2012 | Animal Science Journal2012，83，4： | 1 |
| 6 | Procalcitonin as a marker for the detection of bacteremia and sepsis in the emergency department 显示文摘 | Riedel S Melendez JH An AT | 2011 | Am J Clin Pathol2011，135，2： | 1 |
| 7 | Anatomical considerations of the pediatric ilioinguinal/iliohypogastric nerve block显示文摘 | van Schoor AN Boon JM Bosenberg AT | | 0，，05： | 1 |
| 8 | Procalcitonin as a marker for the detection of bacteremia and sepsis in the emergency department显示文摘 | Riedel S Melendez JH An AT | 2011 | Am J Clin Pathol2011，135，2： | 1 |
| 9 | Detection of fow-level EGFR T790M mutation in lung cancer tissues显示文摘 | Oh JE An CH Yoo N J et at | 2011 | APMIS2011，119，7： | 1 |
| 10 | Terminal cancer patients' and their primary caregivers' attitudes toward hospice/palli- ative care and their effects on actual utilization: A prospec- tive cohort study 显示文摘 | An At/ Lee JK Yun YH | 2014 | Palliat Med2014，28，7： | 1 |
| 11 | r assessing the flbrotie effects of insulin - like growth factor bind- ing proteins 显示文摘 | Yasuoka H Larregina AT Yamaguchi Y Human skin culture as an ex vivo model for | 2008 | Open Rheumatol J2008，2，： | 1 |
| 12 | Solid breast nodules : use of sonography todistinguish between benign and malignant lesions 显示文摘 | Stavors AT Thickman an D Rapp CL | 1995 | Radiology1995，196，： | 1 |
| 13 | Effects of centralleptin administration on blood pressure in normotenive nits显示文摘 | CASTO R M V AN NESS J M OVERTON J M et at | 1998 | Neurosci Lett1998，246，1： | 1 |
| 14 | Thioredoxin glutathione reductase from Schistosoma mansoni: an essential parasite enzyme and a key drug target显示文摘 | Kuntz AN Charvet DC Sayed AA et at | 2007 | PLoS Med2007，6，4： | 1 |
| 15 | Procalcitonin as a marker for the detection of bacteremia and sepsis in the emergency department显示文摘 | Riedel S Melendez JH An AT | | 0，，02： | 1 |
| 16 | Inner Regulations Determine General Trend显示文摘The orientation of bilateral relations in the two countries’ external strategy plays a decisive role in the general trend of bilateral relations. Demand plays the major decisive role in the strategic orientation of external relations. Only when demand is needed, there is a driving force in developing relations. Thus importance will be attached to | Hu Jiping is an Associate Research Professor and Deputy Director at the Division for Northeast Asian Studies, China Institute of Contemporary International Relations. | 2003 | Contemporary International Relations2003，13，11： | 1 |
| 17 | Development of sera reference panels for quality control of ELISA diagnostic kits in Russia显示文摘 | Kanev AN Vorob'eva MS Shalunova NV et at | 1998 | Vestn Ross Akad Med Nauk1998，，3： | 1 |
| 18 | A pilot study of soluble adhesion molecules as surrogate markers for acute liver allograft rejection显示文摘 | Navarro VJ Silver D Langnas AN et at | 1998 | Transplantation1998，65，1： | 1 |
| 19 | The microvesicle as a vehicle for EMMPRIN in tumor-stromal interactions显示文摘 | Sidhu SS Mengistab AT Tauscher AN | 2004 | Oncogene2004，23，4： | 1 |
| 20 | Constrained Total Least-Squares Location Algorithm Using Time-Difference-of-Arrival Measurements显示文摘 | Yang K An J Bu X et at | 2010 | Vehicular Technology IEEE Transactions on2010，59，3： | 1 |
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