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| 1 | Bisphosphonate-related jaw nec-rosis:a team approach management and prevention显示文摘 | Rayman S Almas K Dincer E | 2009 | Int J Dent Hyg2009，7，2： | 1 |
| 2 | On the performance of Ti/SnO2 and Ti/PbO2 anodes in electrochemical degradation of 2-chlorophenol for wastewater treatment显示文摘 | almas S P Renoldi F | 1999 | J Appl Electrochem1999，29，： | 1 |
| 3 | Managing dental fear and anxiety显示文摘 | Rayman S Dincer E Almas K | 2013 | NY State Dent J2013，79，6： | 1 |
| 4 | Bisphosphonate-related jaw necrosis:a team approach management and prevention显示文摘 | Rayman S Almas K Dincer E | 2009 | Int J Dent Hyg2009，7，2： | 1 |
| 5 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 6 | The effect of additional pleural procedures onto recurrence rates on the spontaneous pneumothorax surgery 显示文摘 | Zeybek A Kalemci S Gurunlu Alma O | 2013 | Iran Red Crescent Med J2013，15，2： | 1 |
| 7 | Thermal stability of novolak-type thermosettings made by the condensation of bark and phenol显示文摘 | Alma M H Kelley S S | 2000 | Polym Degrad Stab2000，68，3： | 1 |
| 8 | The impact of common and rare EGFR mutations in response to EGFR tyrosine kinase inhibitors and platinum-based chemotherapy in patients with non-small cell lung cancer显示文摘 | Oscar Arrieta Andrés Felipe Cardona Luis Corrales Alma Delia Campos-Parra Roberto Sánchez-Reyes Eduardo Amieva-Rivera July Rodríguez Carlos Vargas Hernán Carranza Jorge Otero Nikki Karachaliou Horacio Astudillo Rafael Rosell | 2014 | Lung Cancer2014，，： | 1 |
| 9 | Low temperature (7 C) anaerobic treatment of a triehloroethylene-eontaminated wastewater: Microbial community development显示文摘 | Alma S Anne M E Vincent O | 2011 | Water Research2011，45，13： | 1 |
| 10 | Type-D Personality: A Pre- dictor of Quality of Life and Coronary Heart Disease显示文摘 | Saeed T Niazi G S Almas S | 2011 | East Mediterr Health J2011，17，1： | 1 |
| 11 | The impact of back- ground antiepileptic drugs on the efficacy and safety of prega- balin in treating partial-onset seizures:a post hoe analysis of combined clinical trials显示文摘 | Tomson T Almas M Giordano S | 2011 | Epilepsy Res2011，96，12： | 1 |
| 12 | Halitosis among racially diverse popula- tions显示文摘 | Rayman S Almas K | 2008 | Int J Dent Hyg2008，22，6： | 1 |
| 13 | Additional antiprotozoal flavonol glycosides of the aerial parts of Helianthemum glomeratum显示文摘 | FernandoCalzada Alma DeliaAlanís | 2006 | Phytother Res2006，，1： | 1 |
| 14 | Managing dental fear and anxiety显示文摘 | Rayman S Dincer E Almas K | 2013 | NY State Dent J2013，79，1： | 1 |
| 15 | Bisphosphonate-related jaw necrosis: a team approach management and prevention显示文摘 | Rayman S Almas K Dincer E | 2009 | Int J Dent Hyg2009，7，2： | 1 |
| 16 | A Proteomics Study of Brassinosteroid Response in Arabidopsis显示文摘 | DENG Z P ZHANG X TANG W Q OSES-PRIETO J A SUZUKI N GENDORN J M CHEN H J GUAN S H CHALKLEY R J PETERMAN T K ALMA L B WANG Z Y | 2007 | Molecular & Cellular Proteomics2007，6，： | 1 |
| 17 | Bio-accumulation and toxicity of lead(Pb) in Lemna gibba L(duckweed)显示文摘 | Alma S Sobrino María G Miranda Carlos Alvarez | | Journal of Environmental Science and Health Part A0，45，： | 1 |
| 18 | Dynamic and thermal physical processes in low-current sliding contacts显示文摘 | Kharin S N Alma Ata Mikhailov V V | 2001 | International Applied Mechanics2001，44，12： | 1 |
| 19 | A virtual reality based exercise system for hand rehabilitation poststroke显示文摘 | Sergei V Adamovich Alma S Merians Rares Boian | 2004 | Conf Proc IEEE Eng Med Biol Soc2004，7，： | 1 |
| 20 | Synthesis, characterization and antibacterial activity of some triasole Mannich bases carrying diphenylsulfone moieties 显示文摘 | Almajan G L Barbuceanu S F Alma jan E R | 2009 | Eur J Med Chem2009，44，7： | 1 |
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