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| 1 | The correlation of preoperative CT, MR imaging, and clinical staging (FIGO) with histopathology findings in primary cervical carcinoma显示文摘 | Ozsarlak O T~alma W Schepens E | 2003 | Eur Radiol2003，13，10： | 1 |
| 2 | Bisphosphonate-related jaw nec-rosis:a team approach management and prevention显示文摘 | Rayman S Almas K Dincer E | 2009 | Int J Dent Hyg2009，7，2： | 1 |
| 3 | Managing dental fear and anxiety显示文摘 | Rayman S Dincer E Almas K | 2013 | NY State Dent J2013，79，6： | 1 |
| 4 | Bisphosphonate-related jaw necrosis:a team approach management and prevention显示文摘 | Rayman S Almas K Dincer E | 2009 | Int J Dent Hyg2009，7，2： | 1 |
| 5 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 6 | Damage detection in structures:From mode shape to frequency response function methods显示文摘 | Maia N M M Silva J M M Almas E A M | 2008 | Me-chanical Systems and Signal Processing2008，17，3： | 1 |
| 7 | Elutriated acid fermentation of municipal primary sludge 显示文摘 | Alma Y H Speeceb R E | 2006 | Water Research2006，40，11： | 1 |
| 8 | National, regional, and worldwide estimates of preterm birth rates in the year 2010 with time trends since 1990 for selected countries: a systematic analysis and implications显示文摘 | Hannah Blencowe Simon Cousens Mikkel Z Oestergaard Doris Chou Ann-Beth Moller Rajesh Narwal Alma Adler Claudia Vera Garcia Sarah Rohde Lale Say Joy E Lawn | 2012 | The Lancet2012，，9832： | 1 |
| 9 | Dana- age detection in structures:from mode shape to fre- quency response function methods 显示文摘 | Maia N M M Silva J M M Almas E A M | 2003 | Mechanical Systems and Signal Processing2003，17，3： | 1 |
| 10 | Pleurotus eryngii species complex:Sequence analysis andphylogeny based on partial EF1a and RPB2 genes显示文摘 | Alma E Rodriguez Estrada Maria delMar Jimenez-Gasco DanielJ Royse | 2010 | Fungal B-iology2010，114，： | 1 |
| 11 | Immunogenicity of Helicobacter pylori Urease B Protein and DNA Vaccines in a Mouse Model显示文摘 | Rodolfo E Bégué Alma R Cruz Andres Ramgoolam Mary B Breslin | 2007 | Journal of Pediatric Gastroenterology and Nutrition2007，，4： | 1 |
| 12 | Low temperature (7 C) anaerobic treatment of a triehloroethylene-eontaminated wastewater: Microbial community development显示文摘 | Alma S Anne M E Vincent O | 2011 | Water Research2011，45，13： | 1 |
| 13 | Darnge Detection in Structures from Mode Shape to Frequency Response Function Methods 显示文摘 | Maia N M Silva J M Almas E A | 2003 | Mechanical Systems and Signal Processing2003，17，3： | 1 |
| 14 | Glucose Sensor O -GlcNAcylation Coordinates with Phosphorylation to Regulate Circadian Clock显示文摘 | Krista Kaasik Saul Kivim?e Jasmina J. Allen Robert J. Chalkley Yong Huang Kristin Baer Holger Kissel Alma L. Burlingame Kevan M. Shokat Louis J. Ptá?ek Ying-Hui Fu | 2013 | Cell Metabolism2013，，2： | 1 |
| 15 | Managing dental fear and anxiety显示文摘 | Rayman S Dincer E Almas K | 2013 | NY State Dent J2013，79，1： | 1 |
| 16 | Bisphosphonate-related jaw necrosis: a team approach management and prevention显示文摘 | Rayman S Almas K Dincer E | 2009 | Int J Dent Hyg2009，7，2： | 1 |
| 17 | Angiogenie factors and an- giogenesis inhibitors in exudative pleural effusions显示文摘 | Ruiz E Alma n C Alegre J | 2005 | Lung2005，183，3： | 1 |
| 18 | Damage detection in structures: from mode shape to frequency response function methods 显示文摘 | MaiaN M M Silva J M M Almas E A M | 2003 | Mechanical Systems and Signal Processing2003，17，3： | 1 |
| 19 | Immunomodulatory properties of mesenchymal stromal cells 显示文摘 | ALMA J NAUTA WILLEM E FIBBE | 2007 | Blood2007，110，： | 1 |
| 20 | Damagedetection in structures: from mode shape to frequencyresponse function methods 显示文摘 | Maia N M M Silva J M M Almas E A M | 2003 | Mechanical Systems andSignal Processing2003，17，3： | 1 |
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