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您的检索式：作者名="Adrien Guillot"
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| 1 | Kupffer cell restoration after partial hepatectomy is mainly driven by local cell proliferation in IL-6-dependent autocrine and paracrine manners显示文摘Kupffer cells(KCs),which are liver-resident macrophages,originate from the fetal yolk sac and represent one of the largest macrophage populations in the body.However,the current data on the origin of the cells that restore macrophages during liver injury and regeneration remain controversial.Here,we address the question of whether liver macrophage restoration results from circulating monocyte infiltration or local KC proliferation in regenerating livers after partial hepatectomy(PHx)and uncover the underlying mechanisms.By using several strains of genetically modified mice and performing immunohistochemical analyses,we demonstrated that local KC proliferation mainly contributed to the restoration of liver macrophages after PHx.Peak KC proliferation was impaired in Il6-knockout(KO)mice and restored after the administration of IL-6 protein,whereas KC proliferation was not affected in Il4-KO or Csf2-KO mice.The source of IL-6 was identified using hepatocyte-and myeloid-specific Il6-KO mice and the results revealed that both hepatocytes and myeloid cells contribute to IL-6 production after PHx.Moreover,peak KC proliferation was also impaired in myeloid-specific Il6 receptor-KO mice after PHx,suggesting that IL-6 signaling directly promotes KC proliferation.Studies using several inhibitors to block the IL-6 signaling pathway revealed that sirtuin 1(SIRT1)contributed to IL-6-mediated KC proliferation in vitro.Genetic deletion of the Sirt1 gene in myeloid cells,including KCs,impaired KC proliferation after PHx.In conclusion,our data suggest that KC repopulation after PHx is mainly driven by local KC proliferation,which is dependent on IL-6 and SIRT1 activation in KCs. | Yeni Ait Ahmed Yaojie Fu Robim M.Rodrigues Yong He Yukun Guan Adrien Guillot Ruixue Ren Dechun Feng Juan Hidalgo Cynthia Ju Fouad Lafdil Bin Gao | 2021 | Cellular & Molecular Immunology2021，18，9： | 2 |
| 2 | Next-Generation Imaging: New Insights from Multicolor Microscopy in Liver Biology and Disease显示文摘1.Introduction Our current understanding of the complex biology of solid organs,such as the liver,has been tremendously advanced by a wide variety of methodological approaches based on cell biology,biochemistry,molecular biology,and histology.Conventional methods such as the morphological assessment of tissue structure by histology and immunohistochemistry have generated valuable knowledge and revealed improved treatment options for various liver diseases.Furthermore,due to recent technological advances,it has been possible to gain deeper insights into physiological and pathological tissue organization,as well as dynamic processes taking place in vivo.These novel methods include single-cell tar-geted transcriptome analysis,mass spectrometry-based metabolite and protein expression studies,and considerable advances in two-and three-dimensional(2D/3D)imaging.This commentary will briefly review the recent progress that has been made in the field of imaging-based liver research,highlighting the novel possibilities of high-dimensional,multiparametric imaging in two and three dimensions,as well as those of dynamic time-lapse in vitro and in vivo imaging(Fig.1).We will also highlight some potential applications of these techniques for specific research questions in liver biology and disease. | Felix Heymann Adrien Guillot Moritz Peiseler Frank Tacke | 2022 | Engineering2022，8，2： | 0 |
| 3 | Somatic mutant clone screening: scan for novel NASH target genes in mouse liver显示文摘In a landmark study recently published in Cell,Wang et al.demonstrated a novel method of somatic adeno-associated virus(AAV)-transposon in vivo clonal screening(termed MOSAICS),which is capable to generate multiple mutant hepatocyte clones within the same tissue and thereby to scan for non-alcoholic steatohepatitis(NASH)target genes(Fig.1).1 Non-alcoholic fatty liver disease(NAFLD)is the most common liver disease affecting about 20–30%of the global population and associated with metabolic dysfunction.NAFLD can progress to NASH,characterized by liver inflammation,steatosis and hepato-cyte ballooning,and NAFLD/NASH can lead to fibrosis,cirrhosis,and ultimately liver cancer.For decades,researchers have been seeking to understand its pathogenesis and to discover ther-apeutic strategies to treat NAFLD/NASH.However,up to now,there is still no specific pharmacotherapy available. | Tian Lan Adrien Guillot Frank Tacke | 2023 | Signal Transduction and Targeted Therapy2023，8，9： | 0 |
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