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| 1 | Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease显示文摘AIM: To determine the features of Enterococcus that contribute to the development and maintenance of the inflammatory process in patients with inflammatory bowel disease (IBD). METHODS: Multiplex polymerase chain reaction (PCR) was applied to assess the presence of genes that encode virulence factors [surface aggregating protein (asa1), gelatinase (gelE), cytolysin (cylA), extracellular surface protein (esp) and hyaluronidase (hyl)] in the genomic DNA of 28 strains of Enterococcus isolated from the intestinal tissues of children with IBD (n =16) and of children without IBD (controls; n = 12). Additionally, strains with confirmed presence of the gelE gene were tested by PCR for the presence of quorum sensing genes (fsrA, fsrB, fsrC) that control the gelatinase production. Gelatinase activity was tested on agar plates containing 1.6% gelatin. We also analysed the ability of Enterococcus strains to release and decompose hydrogen peroxide (using Analytical Merckoquant peroxide test strips) and tested their ability to adhere to Caco-2 human gut epithelium cells and form biofilms in vitro. RESULTS: A comparison of the genomes of Enterococcus strains isolated from the inflamed mucosa of patients with IBD with those of the control group showed statistically significant differences in the frequency of theasa1 gene and thegelE gene. Furthermore, the cumulative occurrence of different virulence genes in the genome of a single strain ofEnterococcus isolated from the IBD patient group is greater than in a strain from the control group, although no significant difference was found. Statistically significant differences in the decomposition of hydrogen peroxide and adherence to the Caco-2 epithelial cell line between the strains from the patient group and control group were demonstrated. The results also showed that profuse biofilm production was more frequent amongEnterococcus strains isolated from children with IBD than in control strains. CONCLUSION: Enterococcus strains that adhere strongly to the intestinal epithelium, form biofilms and possess antioxidant defence mechanisms seem to have the greatest influence on the inflammatory process. | Edyta Golińska Anna Tomusiak Tomasz Gosiewski Gra yna Wiecek Agnieszka Machul Diana Mikoajczyk Magorzata Bulanda Piotr B Heczko Magdalena Strus | 2013 | World Journal of Gastroenterology2013，19，23： | 8 |
| 2 | TGF superfamily and MMP2,MMP9,TIMP1 genes expression in the endometrium of women with impaired reproduction显示文摘 | SKRZYPCZAK J WIRSTLEIN P MIKO AJCZYK M | | 0，，1： | 1 |
| 3 | Oxidative stress may be a piece in the endometriosis puzzle显示文摘 | Szczepańska M Ko?lik J Skrzypczak J Miko?ajczyk M | 2003 | Fertil Steril2003，79，： | 1 |
| 4 | Influence of fibre formation conditions on the properties of nanocomposite PAN fibres containing nanosilver显示文摘 | Miko~ajczyk T Szparaga G | 2009 | Fibres Textiles Eastern Europe2009，17，4： | 1 |
| 5 | A scale and affine invariant inter-est point detectors 显示文摘 | Miko|ajczyk K Schmid C | 2004 | International Journal of Computer Vi-seion2004，60，1： | 1 |
| 6 | Effects of Aroclor 1254 on LH and 17,2013-P secretion in female Prussian carp (Carassius gibelio Bloch ) in the spawning season显示文摘 | Socha M Soko~owska-Miko~ajczyk M Szczerbik P | 2013 | Czech J Anita Sci2013，58，8： | 1 |
| 7 | A comparison of affine region detectors显示文摘 | MIKOI AJCZYK K TUYTELAARS T SCHMID C | 2005 | Inter- national Journal of Computer Vision2005，65，12： | 1 |
| 8 | The assessment of LIF in uterine flushing-a possible new diagnostic tool in states of impaired fertility显示文摘 | Miko|/ajczyk M Skrzypczak J Szymanowski K | 2003 | Reprod Biol2003，3，3： | 1 |
| 9 | The influenceof mianserin on TNF, IL-6 and IL-10 serum levels in rats un-der chronic mild stress显示文摘 | Manikowska K Miko ajczyk M Miko ajczak P | 2014 | Pharmacol Rep2014，66，1： | 1 |
| 10 | Scale & affine invariant interest point detectors显示文摘 | MIKOI AJCZYK K SCtlMID C | 2004 | International Journal of Computer Vision2004，60，1： | 1 |
| 11 | Multifunctional algi- hate fibres with anti-bacterial properties显示文摘 | Mik~ajczyk T Wc~owska-Czapnik D | 2005 | Fibres Text East Eur2005，3，51： | 1 |
| 12 | Effect of low-energy laser irradiation and antioxidant supplementation on cell apoptosis during skeletal muscle post-injury regeneration in pigs显示文摘 | Otrocka-Domagaa I Mikoajczyk A Pa z'dzior-Czapula K | 2014 | Pol J Vet Sci2014，18，3： | 1 |
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