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| 1 | Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells显示文摘BACKGROUND Human adipose-derived stromal/stem cells(hASCs)are one of the most useful types of mesenchymal stromal/stem cells,which are adult multipotent cells with great therapeutic potential for the treatment of several diseases.However,for successful clinical application,it is critical that high-quality cells can be obtained.Diverse factors seem to be able to influence cell quality and performance,especially factors related to donors’intrinsic characteristics,such as age.Nevertheless,there is no consensus regarding this characteristic,and there is conflicting information in the literature.AIM To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young donors.METHODS hASCs were harvested from liposuctioned adipose tissue obtained from female donors(aged 20-70 years).Cells were distributed into two groups according to age range:old hASCs(oASCs,≥55 years,n=9)and young hASCs(yASCs,≤35 years,n=9).For each group,immunophenotypic characterization was performed by flow cytometry.Population doubling time was assessed over seven days.For adipogenic potential evaluation,lipid deposits were assessed after 7 d,14 d and 21 d of adipogenic induction.Osteogenic potential was verified by analyzing cell mineralization after 14 d,21 d and 28 d of osteogenic induction.mRNA expression of PPARγ2,CEBPA and Runx2 were detected by quantitative reverse transcription polymerase chain reaction.RESULTS hASCs were successfully obtained,cultured,and grouped according to their age:yASCs(26.33±4.66 years old)and oASCs(64.78±4.58 years old).After maintenance of the cells in culture,there were no differences in morphology between cells from the young and old donors.Additionally,both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal cells.The average doubling time indicated that yASCs(4.09±0.94 d)did not significantly differ from oASCs(4.19±1.29 d).Concerning differentiation potential,after adipogenic and osteogenic induction,yASCs and oASCs were able to differentiate to greater levels than the noninduced control cells.However,no differences were found in the differentiation efficiency of yASCs and oASCs in adipogenesis or osteogenesis.Additionally,the mRNA expression of PPARγ2,CEBPA and Runx2 were similar in yASCs and oASCs.CONCLUSION Our findings suggest that age does not seem to significantly affect the cell division or adipogenic or osteogenic differentiation ability of adipose-derived stem cells isolated from lipoaspirates. | Cintia DS Horinouchi María Julia Barisón Anny W Robert Crisciele Kuligovski Alessandra M Aguiar BrunoDallagiovanna | 2020 | World Journal of Stem Cells2020，12，12： | 2 |
| 2 | Pressor effects of electrical stimulation of medial prefrontal cortex in unanesthetized rats显示文摘 | TAVARES R F ANTUNES-RODRIGUES J DE AGUIAR CORREA F M | 2004 | Journal of Neuroscience Research2004，77，4： | 1 |
| 3 | Lamellar spacing selection in a directionally solidified Sn-Se eutectic alloy显示文摘 | Aguiar M R Caram R | 1997 | J Crystal Growth1997，174，： | 1 |
| 4 | D-opti- mal designs 显示文摘 | De Aguiar P 17 Bourguignon B Khots M S et el | 1995 | Chemometr Intdl Lab1995，30，2： | 1 |
| 5 | C-reactive protein clinical applications and proposals for a rational use显示文摘 | Aguiar FJ Ferreira-JOnior M Sales MM | 2013 | Rev Assoc Med Bras2013，59，1： | 1 |
| 6 | Assessment of the enzymatic hydrolysis profile of cellulosic substrates based on reducing sugar release显示文摘 | Silveira M H L Aguiar R S Siika-aho M | 2014 | Bioresource Technology2014，151，： | 1 |
| 7 | Physicochemical studies on the interaction between N - Decanoyl - N - methylglucamide and bovine serum albumin 显示文摘 | RUIZ C C HIERREZUELO J M AGUIAR J | 2007 | Biomacromolecules2007，8，8： | 1 |
| 8 | Short-Term Load Forecasting for Microgrids Based on Artificial Neural Networks显示文摘 | HERNANDEZ L BALADRON C AGUIAR J M | 2013 | Energies2013，6，3： | 1 |
| 9 | Fluid dynamics study of the influence of the position of the feed in fabric filters 显示文摘 | Rocha S M S Vieira L G M Aguiar M L | 2009 | Computer Aided Chemical Engineering2009，27，： | 1 |
| 10 | Slowmagic-angle coil spinning: a high-sensitivity and high-resolution NMR strategy for microscopic biologicalspecimens显示文摘 | WONG A AGUIAR P M SAKELLARIOU D | 2010 | Magnetic Resonance in Medicine2010，63，2： | 1 |
| 11 | Unplanned extubation in the neonatal ICU:a systematic review,critical appraisal,and evidence-based recommendations显示文摘 | Silva P S Reis M E Aguiar V E | 2013 | Respir Care2013，58，7： | 1 |
| 12 | Quadri-algebras显示文摘 | AGUIAR M LODAY J L | 2004 | Journal of Pure and Applied Algebra2004，191，： | 1 |
| 13 | Litter effects on plant regenera- tion in arid lands: a complex balance between seed retention, seed longevity and soil-seed contact 显示文摘 | ROTUNDO J L AGUIAR M R | 2005 | Journal of Ecology2005，93，4： | 1 |
| 14 | Friedel-Crafts acylaiton of anisole with acetic anhydride over silica-supported hetempolyphostungstic acid (HPW/SiO2 )显示文摘 | CARDOSO L A M ALVES W It GONZAGA A R E AGUIAR L M G ANDRADE H M C | 2004 | J Mol Catal A2004，209，12： | 1 |
| 15 | Internal suture technique for improving projection and stability in secondary gluteoplasty显示文摘 | JAIMOVICH C A ALMEIDA M W AGUIAR L F | 2010 | Aesthet Surg J2010，30，3： | 1 |
| 16 | Encapsulation of insulin-cyelodextrin complex in PLGA microspheres: a new approach for prolonged puhnonary insulin delivery显示文摘 | Aguiar M M Rodrigues J M Silva Cunba A | 2004 | Microencapsul2004，21，5： | 1 |
| 17 | Best cases from the AFIP:congenital intracranial teratoma显示文摘 | SANDOW B A DORY C E AGUIAR M A | 2004 | Radiographics2004，24，4： | 1 |
| 18 | Nuclear inosi- tol 1,4,5-trisphosphate is a necessary and conserved sig- nal for the induction of both pathological and physiological cardiomyocyte hypertrophy 显示文摘 | Arantes LA Aguiar C J Amaya M J | 2012 | J Mol Cell Cardiol2012，53，4： | 1 |
| 19 | Antimalarial activity and mechanisms of action of two novel 4- aminoquinolines against chloroquine-resistant parasites 显示文摘 | AGUIAR A C C SANTOS R D M FIGUEIREDO F J B | 2012 | PLoS One2012，7，37： | 1 |
| 20 | Microwave Promoted Suzuki Reactions between Aroyl Chlorides and Boronic Acids Catalyzed by Het- erogeneous and Homogeneous Phosphine-free Palla- dium Catalysts 显示文摘 | DANIELA DE L M AGUIAR L C ANTUNES O A | 2011 | J of Organomet Chem2011，696，1516： | 1 |
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