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| 1 | Virulence and potential pathogenicity of coccoid Helicobacter pylori induced by antibiotics显示文摘AIM To explore the virulence and the potential pathogenicity of coccoid Helicobacter pylori (H. pylori) transformed from spiral form by exposure to antibiotic.METHODS Three strains of H. pylori, isolated from gastric biopsy specimens of confirmed peptic ulcer, were converted from spiral into coccoid from by exposure to metronidazole.Both spiral and coccoid form of H. pylori were tested for the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells, and the differences of the protein were analysed by SDS-PAGE and Western blot,The mutation of the genes including ureA, ureB,hpaA; vacA and cagA, related with virulence,was detected by means of PCR and PCR-SSCP.RESULTS In the coccoid H. pylori, the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells alldecreased. In strain F44, the rate and index of adherence reduced from 70.0% ± 5.3% to 33% ±5.1% and from 2.6 ±0.4 to 0.96 ±0.3 (P<0.01),respectively. The invasion of coccoid H. pylori into Hep-2 cell could be seen under electronmicroscope. SDS-PAGE showed that the content of the protein with the molecular weight over Mr74 000 decreased, and the hybriditional signal in band Mr 125 000 weakened, while the band Mr 110000 and Mr63000 strengthened in coccoid H. pylori as shown in Western blot. The results of PCR were all positive, and PCR-SSCP indicated that there may exist the point mutation in gene hpaA or vacA.CONCLUSION The virulence and the proteins with molecular weight over Mr74 000 in coccoid H. pylori decrease, but no deletion exists in amplification fragments from ureA, ureB, hpaA,vacA and cagA genes, suggesting that coccoid H. pylori may have potential pathogenicity. | Fei Fei She1 Dong Hui Su1 Jian Yin Lin2 Lin Ying Zhou3 1Department of Microbiology, Fujian Medical University. Fuzhou 350004, Fujian Province, China2Department of Molecular Medicine, Fujian Medical University, Fuzhou 350004, Fujian Province, China 3Laboratory of Electron Microscope, Fujian Medical University, Fuzhou 350004. Fujian Province. ChinaFei Fei She. graduated from Fujian Medical University as a postgraduate in 1991, now associate professor of microbiology and immunology, specialized in molecular biology of pathogen, having 15 papers published. | 2001 | World Journal of Gastroenterology2001，7，2： | 18 |
| 2 | 上海市城乡高血压患病率动态对比分析显示文摘按全国统一标准和方法,对上海城乡各3个随机抽样点15岁及以上成人21189名进行血压普查,校正应答率91.85%.结果:①临界、确诊和合并(临界+确诊)患病率分别为6.38%、6.31%、12.69%;城乡标化患病率分别为11.05%、6.59%,患病率骤升年龄城市较早5岁、患病率及血压水平城乡相差10~15年.②男性在55岁前患病率高于女性,65岁后相反;血压值男性在46岁后、女性在45岁后城高于乡,40岁前在男性城乡差别不大,而女性反乡高于城.③城市合并标化患病率本次与1979年普查结果间无明显差别.而农村则本次为1979年的181.04%,且患病率骤升年龄提前5岁.城乡标化患病率差别从1979年的2.2倍降至本次的0.68倍. | 赵光胜 袁晓源 上海市1991年高血压抽样调查组 | 1992 | 中国慢性病预防与控制1992，，1： | 12 |
| 3 | 北京市高血压流行病学调查分析显示文摘北京市高血压患病率在国内属高发区之一,本研究调查了北京地区所有区县45558人高血压患病率,结果:①临界、确诊、和合并(临界+确诊)高血压标化患病率分别为9.26%,10.46%,19.72%.②高血压患病率于35岁以后明显增加,45岁以前患病率男性高于女性,45岁以后女性患病率上升加快,至55岁以后超过男性.③城市临界、确诊、合并高血压患病率分别为8.92%,8.97%,17.82%,农村分别为11.72%,9.56%、21.28%,农村明显高于城市,.④不同职业合并高血压患病率有明显差异,工人、农民较高,19.66%,19.56%,科技人员最低14.99%,⑤教育水平、卫生知识水平与高血压患病率及血压水平呈负相关. | 胡长梅 高晓兰 张小明 北京市1991年高血压抽样调查组 | 1993 | 中国慢性病预防与控制1993，1，6： | 9 |
| 4 | 高氯酸·五氨·[2-(5-氰基四唑酸根)]合钴(Ⅲ) 的制备[J]显示文摘 | 盛涤伦 刘晓慧 于天义 王锦明 吕巧莉 马凤娥. 高氯酸五氨·合钴(Ⅲ)的制备. 火工品. 1991 (04) | 1991 | 火工品1991，，4： | 4 |
| 5 | Phosphonium and arsonium ylides(?)——ⅩⅦ.A facile synthesis of methyl 2,6-bisperfluoroalkylbenzoates via acyclic precursors显示文摘The title compounds 6a—6f were prepared with high yield via intramolecular Wittigreaction of methyl 3-perfluoroalkyl-6-perfluoroacyl-2-triphenylphosphoranylidenehex-3,5-dienoates(5a—5i)which were obtained from the reaciton of 3-perfluoroacylprop-2-enylidenetriphenylphos-phoranes(3a—3c)with methyl perfluoroalkynoates(4a—4c). | DING,Wei-Yu CAO,Wei-Guo XU,Zhen-Rong SHI,Zhi-Jian YAO,Yuan Department of Chemistry,Shanghai University of Science and Technology,Shanghai 201800 For part XVI of the series,see J.Chem.Soc.,Perkin Trans.1,1369(1991). | 1993 | Chinese Journal of Chemistry1993，11，1： | 3 |
| 6 | Economic growth in a cross section of countries 显示文摘 | 1991 | 1991 | The Quarterly Journal of Economics1991，106，2： | 1 |
| 7 | Formation of nitrate and ammonium-ions in titanium-dioxide mediated photocatalytic degradation of organic-compounds compounds containing nitrogen-atoms 显示文摘 | McEvoy S R Matthews R W 1991 | 1991 | Environ Sci Technol1991，25，： | 1 |
| 8 | Physiology of inorganic C acquisition and implication for resource use efficiency by marine phytoplankton:relation to increased CO2 and temperature显示文摘 | Raven J A 1991 | 1991 | Plant Cell Environ1991，14，： | 1 |
| 9 | A new species of Larix(Pinaceae) from the early Tertiary of Axel Heiberg Island,Arctic Canada显示文摘 | LePage BA Basinger JF 1991 | 1991 | Rev Palaeobot Palynol1991，70，： | 1 |
| 10 | Measuring the information content of stock trades显示文摘 | Hasbrouck Joel 1991 | 1991 | Journal of Finance461991，，： | 1 |
| 11 | Isolation and characterization of a cDNA clone encoding an alternative oxidase protein of Sauromatum guttatum(Schott)显示文摘 | Rhoads DM McIntosh L 1991 | 1991 | Proc Nat Acad Sci USA1991，88，： | 1 |
| 12 | Chemical composition of emissions from urban sources of fine aerosols 显示文摘 | Markowski G R Cass G R 1991 | 1991 | Environ Sci Technol1991，25，： | 1 |
| 13 | Qualitative and quantitative analysis of the phenelic constituents from Orthosiphon aristatus显示文摘 | Wahono S Peter P Victor W 1991 | 1991 | Planta Med1991，57，： | 1 |
| 14 | BIOCLIMa bioclimate analysis and prediction system显示文摘 | Busby JR 1991 | 1991 | Plant Protection Quarterly1991，6，1： | 1 |
| 15 | Functional and symbolic congruence approaches to consumer satisfaction/dissatisfaction in tourism 显示文摘 | Chon K S Olsen M D (1991) | 1991 | Journal of the International Academy of Hospitality Research1991，218，： | 1 |
| 16 | Tourism,welfare and de-industrialization in a small open economy显示文摘 | Copeland B R( 1991 ) | 1991 | Economica New Series 58(232)1991，，： | 1 |
| 17 | Policies for tourism development in Kenya 显示文摘 | Dieke P U C ( 1991 ) | 1991 | Annals of Tourism Research1991，，： | 1 |
| 18 | Degradation of halogenated phenols in anoxic marine sediments显示文摘 | Abrahamsson K 1991 | 1991 | Marine Pollution Bulletin1991，22，： | 1 |
| 19 | Phylogenetic relationships within North American Astragalus(Fabaceae)显示文摘 | Sanderson MJ 1991 | 1991 | Systematic Botany1991，16，： | 1 |
| 20 | Marketing your community:Image analysis of norfolk 显示文摘 | Chon K S Weaver P A Kim C Y( 1991 ) | 1991 | The Cornell Hotel and Restaurant Administration Quarterly1991，3137，： | 1 |
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