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| 1 | Effect of compound rhodiola sachalinensis A Bor on CCI4-induced liver fibrosis in rats and its probable molecular mechanisms显示文摘AIM: To explore the anti-fibrotic effect of a traditional Chinese medicine, compound rhodiola sachalinensis A Bor on CCl4-induced liver fibrosis in rats and its probable molecular mechanisms. METHODS: Ninety healthy male SD rats were randomly divided into three groups: normal group (n=-10), treatment group of compound rhodiola sachalinensis A Bor (n=-40) and CCl4-induced model group (n=40). The liver fibrosis was induced by CCl4 subcutaneous injection. Treatment group was administered with compound rhodiola sachalinensis A Bor (0.5 g/kg) once a day at the same time. Then the activities of several serum fibrosis-associated enzymes: alanine aminotransferase (ALT), aspartate aminotransferase (AST), N-acetyl-beta-D-glucosaminidase (β-NAG) and the levels ofserum procollagen Ⅲ (PCⅢ), collagen Ⅳ (CⅣ), hyaluronic acid (HA) were assayed. The histooathol(mical chanaes were observed with HE, VG and Masson stain. The expression of TGF-β1 mRNA,αl (I) mRNA and Na+/Ca2+ exchanger (NCX ) mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) in situ.RESULTS: Compound rhodiola sachalinensis A Bor significantly reduced serum activities of ALT, AST, β-NAG and decreased the levels of PCⅢ, CⅣ, HA, improved the liver histopathological changes, inhibited the expression of TGF-β1 mRNA, α(1) mRNA and Na+/Ca2+ exchanger mRNA in rats. CONCLUSION: Compound rhodiola sachalinensis A Bor can intervene in CCI4-induced liver fibrosis in rats, in which potential mechanisms may be decreasing the production of TGF-β1, reducing the production of collagen, preventing the activation of hepatic stellate cell (HSC) and inhibiting theexpression of TGF-β1 mRNA, αl(I) mRNA and Na+/Ca2+ exchanger mRNA. | Chun-TaoLei Ming-DeJiang Xiao-BinChen YongZhang HuiXu ZhaoWang Xiao-LingWU Wei-ZhengZeng Pi-LongWang | 2003 | World Journal of Gastroenterology2003，9，7： | 21 |
| 2 | Microscopic spread of low rectal cancer in regions of mesorectum:Pathologic assessment with whole-mount sections显示文摘AIM: To assess the microscopic spread of low rectal cancer in mesorectum regions to provide pathological evidence for the necessity of total mesorectal excision (TME). METHODS: A total of 62 patients with low rectal cancer underwent low anterior resection and TME, surgical specimens were sliced transversely on the serial embedded blocks at 2.5 mm interval, and stained with hematoxylin and eosin (HE). The mesorectum on whole-mount sections was divided into three regions: outer region of mesorectum (ORM), middle region of mesorectum (MRM) and inner region of mesorectum (IRM). Microscopic metastatic foci were investigated microscopically on the sections for the metastatic mesorectal regions, frequency, types, involvement of lymphatic vessels and correlation with the original rectal cancer. RESULTS: Microscopic spread of the tumor in mesorectum and ORM was observed in 38.7% (24/62) and 25.8% (16/62) of the patients, respectively. Circumferential resection margin (CRM) with involvement of microscopic metastaticfoci occurred in 6.5% (4/62) of the patients, and distal mesorectum (DMR) involved was 6.5% (4/62) with the spread extent within 3 cm of low board of the main lesions. Most (20/24) of the patients with microscopic metastasis in mesorectum were in Dukes C stage. CONCLUSION: Results of the present study support that complete excision of the mesorectum without destruction of the ORM is essential for surgical management of low rectal cancer, an optimal DMR clearance resection margin should be no less than 4 cm, further pathologic assessment of the regions in extramesorectum in the pelvis is needed. | ZhaoWang Zong-GuangZhou CunWang Gao-PingZhao You-DaiChen Hong-KaiGao Xue-LianZheng RongWang Dai-YunChen Wei-PingLiu | 2004 | World Journal of Gastroenterology2004，10，20： | 8 |
| 3 | Pathological study of distal mesorectal cancer spread to determine a proper distal resection margin显示文摘AIM: Local recurrence after curative surgical resection for rectal cancer remains a major problem. Several studies have shown that incomplete removal of cancer deposits in the distal mesorectum contributes a great share to this dismal result. Clinicopathologic examination of distal mesorectum in lower rectal cancer was performed in the present study to assess the incidence and extent of distal mesorectal spread and to determine an optimal distal resection margin in sphincter-saving procedure.METHODS: We prospectively examined sepecimens from 45 patients with lower rectal cancer who underwent curative surgery. Large-mount sections were performed to microscopically observe the distal mesorectal spread and to measure the extent of distal spread. Tissue shrinkage ratio was also considered. Patients with involvement in the distal mesorectum were compared with those without involvement with regard to clinicopathologic features.RESULTS: Mesorectal cancer spread was observed in 21patients (46.7%), 8 of them (17.8%) had distal mesorectal spread. Overall, distal intramural and/or mesorectal spreads were observed in 10 patients (22.2%) and the maximum extent of distal spread in situ was 12 mm and 36 mm respectively. Eight patients with distal mesorectal spread showed a significantly higher rate of lymph node metastasis compared with the other 37 patients without distal mesorectal spread (P = 0.043).CONCLUSION: Distal mesorectal spread invariably occurs in advanced rectal cancer and has a significant relationship with lymph node metastasis. Distal resection margin of 1.5 cm for the rectal wall and 4 cm for the distal mesorectum is proper to those patients who are arranged to receive operation with a curative sphincter-saving procedure for lower rectal cancer. | Gao-PingZhao Zong-GuangZhou Wen-ZhangLei Yong-YangYu CunWang ZhaoWang Xue-LianZheng RongWang | 2005 | World Journal of Gastroenterology2005，11，3： | 7 |
| 4 | Effects of chebulinic acid on differentiation of human leukemia K562 cells显示文摘AIM: To study effects of chebulinic acid on erythroid and megakaryocytic differentiation in K562 cells. METHODS: The benzidine staining method was used to evaluate hemoglobin synthesis; the expression of erythroid specific glycophorin A (GPA) protein and megakaryocytic surface marker CD61 was determined by flow cytometry using fluorescence labeled antibodies; erythroid and megakaryocytic mRNA expression was analyzed by RT-PCR. RESULTS: During erythroid differentiation induced by butyric acid (BA) or hemin, chebulinic acid not only inhib- ited the hemoglobin synthesis of BA- and hemin-treated K562 cells in concentration-dependant manner with IC50 of 4 μmol/L and 40 μmol/L respectively, but also inhibited another erythroid differentiation marker acetylcholinesterase at the concentration of 50 μmol/L in the cells either treated or untreated with each erythroid differentiation inducers, whereas chebulinic acid 50 μmol/L did not change GPA protein expression in these cells significantly. When K562 cells were treated with TPA 50 μg/L for 72 h to induce megakaryocytic differentiation, the presence of chebulinic acid 50 μmol/L slightly provoked the decrease of GPA protein expression induced by TPA. Chebulinic acid did not change the TPA-induced CD61 expression at the same concentration. Chebulinic acid also reduced the mRNA levels of erythroid relative genes including γ-globin, PBGD, NF-E2, and GATA-1 genes in K562 cells either treated or untreated with BA, whereas chebulinic acid upregulated the mRNA levels of GATA-2 transcription factor in these cells. CONCLUSION: Chebulinic acid had inhibitory effect on erythroid differentiation likely through changing transcriptional activation of differentiation relative genes, which suggests that chebulinic acid or other tannins might influence the efficiency of some anti-tumor drugs-induced differentiation or the hematopoiesis processes. | Zong-chunYI ZhaoWANG Hai-xiaLI Ming-jieLIU Rong-congWU Xiao-huiWANG | 2004 | Acta Pharmacologica Sinica2004，25，2： | 4 |
| 5 | Synthesis and application of biocompatible nontoxic nanoparticles for reclamation of Ce^3+ from synthetic wastewater: Toxicity assessment, kinetic, isotherm and thermodynamic study显示文摘The aim of present study is to synthesize forsterite nanoparticles(FRST) for the reclamation of cerium ions(Ce^(3+)) from synthetic wastewater.The aim to synthesize FRST nanoparticles is due to its biocompatible and nontoxic nature.The formation of nanoparticles with average diameter of 58 nm was confirmed by TEM analysis.SEM images of bare FRST nanoparticles show a heterogeneous surface with porous nature.BET surface area of FRST nanoparticles is calculated to be 33.69 m^2/g.The significant uptake of Ce^(3+) ions can be obtained for all the selected concentrations(25-150 mg/L) within 2 h of adsorbent—adsorbate interaction.The pH study shows that by increasing pH from acidic to alkaline range,higher removal can be achieved.Temperature study demonstrates the endothermic nature of Ce^(3+)adsorption.The value of sticking probability suggests very high sticking probability of Ce^(3+) ion for FRST nanoparticles.Ce^(3+) uptake is favored by higher temperature and with the increase in temperature from298 to 328 K,Langmuir adsorption capacity increases from 36.45 to 42.99 m^2/g.Applicability of FRST nanoparticles was also investigated for other light and heavy rare earth elements in single solute and multisolute systems,FRST nanoparticles show the significant removal of divalent metallic pollutants as well.The assessment of chemical toxicity of treated wastewater was carried out with the bioluminescent photobacterium(Vibrio fischeri) and decreased toxicity was observed in treated water samples.The outcome of present study suggests that the FRST nanoparticles can be efficiently utilized for the removal of Ce^(3+) ions and a wide range of other pollutant species as well. | Varsha Srivastava Sidra Iftekhar ZhaoWang Indu Babu Mika Sillanpaa | 2018 | Journal of Rare Earths2018，36，9： | 2 |
| 6 | Pituitary adenylate cyclase activating-peptide and its receptor antagonists in development of acute pancreatitis in rats显示文摘AIM: Pituitary adenylate cyclase activating-peptide (PACAP) is a late member of the secretin/glucagon/vasoactive intestinal peptide (VIP) family of brain-gut peptides. It is unknown whether PACAP takes part in the development of acute pancreatitis and whether PACAP or its antagonists can be used to suppress the progression of acute pancreatitis.We investigated the actions of PACAP and its receptor antagonists in acute pancreatitis on rats.METHODS: Acute pancreatitis was induced in rats with caerulein or 3.5% sodium taurocholate. The rats were continuously infused with 5-30 μg/kg PACAP via jugular vein within the first 90 min, while 10-100 μg/kg PACAP6-27 and (4-Cl-D-Phe6, Leu17) VIP (PACAP receptor antagonists) were intravenously infused for 1 h. Biochemical and histopathological assessments were made at 4 h after infusion. Pancreatic and duodenal PACAP concentrations were determined by enzyme-linked immunosorbent assay (ELISA). Chinese ink-perfused pancreas was fixed, sectioned and cleared for counting the functional capillary density.RESULTS: PACAP augmented caerulein-induced pancreatitis and failed to ameliorate sodium taurocholate-induced pancreatitis. ELISA revealed that relative concentrations of PACAP in pancreas and duodenum were significantly increased in both sodium taurocholate- and caeruleininduced pancreatitis compared with those in normal controls.Unexpectedly, PACAP6-27 and (4-Cl-DPhe6, Leu17) VIP could induce mild acute pancreatitis and aggravate caeruleininduced pancreatitis with characteristic manifestations of acute hemorrhagic/necrotizing pancreatitis. Functional capillary density of pancreas was interpreted in the context of pancreatic edema, and calibrated functional capillary density (calibrated FCD), which combined measurement of functional capillary density with dry weight/wet weight ratio, was introduced. Hyperemia or congestion, rather than ischemia, characterized pancreatic microcirculatory changes in acute pancreatitis.CONCLUSION: PACAP may take part in thepathogenesis of acute pancreatitis in rats. The two PACAP receptor antagonsits might act as partial agonists. Calibrated functional capillary density can reflect pancreatic microcirculatory changes in acute pancreatitis. | You-DaiChen Zong-GuangZhou ZhaoWang Hong-KaiGao Wen-WeiYan CurtWang Gao-PingZhao,Xiao-HuiPeng | 2005 | World Journal of Gastroenterology2005，11，4： | 2 |
| 7 | Differences in platelet endothelial cell adhesion molecule-1 expression between peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis显示文摘AIM: To investigate the changes of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonuclear leukocytes (PMNs) in peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis (AEP).METHODS: Fifty Wistar rats were randomly divided into control group (n=10) and AEP group (n=40). A model of AEP was established by subcutaneous injection of cerulein 5.5 and 7.5 μg/kg at 0 and 1 h after the beginning of experiment respectively. PECAM-1 expression on PMNs from splenic vein and inferior vena cava was determined by RT-PCR at mRNA level and determined by flow cytometry at protein level.RESULTS: In experimental rats, an increased PECAM-1mRNA expression was seen from 4 to 8 h of AEP in peripheral circulation (0.77±0.25%, 0.76±0.28%, 0.89±0.30%,1.00±0.21% ), while in pancreatic microcirculation,expression decreased from 2 h and reached the lowest level at 6 h of AEP (0.78±0.29%, 0.75±0.26%, 0.62±0.28%,0.66±0.20%). There were significant differences at 8-h time point of AEP between peripheral circulation and pancreatic microcirculation (1.00±0.21% vs0.66±0.20%, P<0.05).Meanwhile,the difference at protein level was also found.CONCLUSION: A reverse expression of PECAM-1 on PMNs was found between peripheral circulation and pancreatic microcirculation, suggesting that inhibition of PECAM-1expression may improve the pathological change of AEP. | Hong-KaiGao Zong-GuangZhou Fang-HaiHan You-QinChert Wen-WeiYan TaoHe CunWang ZhaoWang | 2005 | World Journal of Gastroenterology2005，11，5： | 2 |
| 8 | Resolution of biotin intermediate lactone by enzyme-catalyzed stereoselective lactonization in organic solvent显示文摘 | Jian- Yong Zheng ZhaoWang Qing Zhu | 2009 | Joumal of Molecular Catalysis B : Enzymatic2009，56，1： | 1 |
| 9 | Echinacoside sup-presses cellular senescence of human fibroblastic cells by down-regulation of p53显示文摘 | HuiZhu CongCheng ChiZhang ZhaoWang | | 0，，： | 1 |
| 10 | Oral Preparations of Chinese materiamedicabysemi - bionicextraetion 显示文摘 | Zhang Zhaowang Sun Xiumei | 1996 | ChemicalAbstraetsVol1996，，： | 1 |
| 11 | Supernova survey system in beijing astronomical Observatory (I)显示文摘 | Yulei Qiu Weidong Li Zhaowang Zhao Qiyuan Qiao Yong Rao Jingyao Hu Qibin Li | 1999 | Science in China Series A: Mathematics1999，，2： | 1 |
| 12 | Oral preparation of Chinese material medicine by semi-bionic extraction 显示文摘 | Zhang zhaowang Sun Xiumei | 1996 | Chem Abstracts1996，124，： | 1 |
| 13 | Oral Preparations of Chinese materia medica by semi-bionic extraction显示文摘 | Zhang zhaowang Sun xiumei | 1996 | Chemical Abstracts1996，1996，： | 1 |
| 14 | A simulation-based multi-objective genetic algorithm (SMOGA) procedure for BOT network design problem显示文摘 | Anthony Chen Kitti Subprasom Zhaowang Ji | 2006 | Optimization and Engineering2006，，3： | 1 |
| 15 | Solving the overlapping problem in route choice with paired combinatorial logit model显示文摘 | Chen A Kasikitwiwat P Zhaowang J | 2003 | Transportation Research Record:Journal of the Transportation Research Board2003，1857，： | 1 |
| 16 | Mean- variance Model for the Build-operate-transfer Scheme under Demand Uncertainty 显示文摘 | CHEN A SUBPRASOM K JI Zhaowang | 2003 | Transportation Research Record2003，1857，： | 1 |
| 17 | A Simulationbased Multi-objective Genetic Algorithm (SMOGA) Procedure for BOT Network Design Problem 显示文摘 | CHEN A SUBPRASOM K JI Zhaowang | 2006 | Optimization and Engineering2006，7，8： | 1 |
| 18 | Mean variancemodel {or the build-operate-transfer scheme under demand uncertMn显示文摘 | Chen A Subprasom K JI Advanced Transportation Zhaowang | 2003 | Transportation Research Re cord2003，，1857： | 1 |
| 19 | Coupling simulation algorithm of a rotating flat-plate blade with particle dampers under centrifugal forces显示文摘 | Xia Zhaowang Liu Xiandong Shan Yingchun | 2011 | Journal of Vibration and Acoustics Transactions of the ASME2011，133，4： | 1 |
| 20 | Coupling simulation algorithm of discrete element method and finite element method for particle damper显示文摘 | Xia Zhaowang Liu Xiandong Shan Yingchun | 2009 | Journal of Low Frequency Noise Vibration and Active Control2009，28，3： | 1 |
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