|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Profile improvement during CO_2 flooding in ultra-low permeability reservoirs显示文摘Gas flooding such as CO2 flooding may be effectively applied to ultra-low permeability reservoirs, but gas channeling is inevitable due to low viscosity and high mobility of gas and formation heterogeneity. In order to mitigate or prevent gas channeling, ethylenediamine is chosen for permeability profile control. The reaction mechanism of ethylenediamine with CO2, injection performance, swept volume, and enhanced oil recovery were systematically evaluated. The reaction product of ethylenediamine and CO2 was a white solid or a light yellow viscous liquid, which would mitigate or prevent gas channeling. Also, ethylenediamine could be easily injected into ultra-low permeability cores at high temperature with protective ethanol slugs. The core was swept by injection of 0.3 PV ethylenediamine. Oil displacement tests performed on heterogeneous models with closed fractures, oil recovery was significantly enhanced with injection of ethylenediamine. Experimental results showed that using ethylenediamine to plug high permeability layers would provide a new research idea for the gas injection in fractured, heterogeneous and ultra-low permeability reservoirs. This technology has the potential to be widely applied in oilfields. | Zhao Fenglan Zhang Lei Hou Jirui Cao Shujun | 2014 | Petroleum Science2014，11，2： | 12 |
| 2 | RhMYB108,an R2R3-MYB transcription factor,is involved in ethylene-and JA-induced petal senescence in rose plants显示文摘Rose(Rosa hybrida)plants are major ornamental species worldwide,and their commercial value greatly depends on their open flowers,as both the quality of fully open petals and long vase life are important.Petal senescence can be started and accelerated by various hormone signals,and ethylene is considered an accelerator of petal senescence in rose.To date,however,the underlying mechanism of signaling crosstalk between ethylene and other hormones such as JA in petal senescence remains largely unknown.Here,we isolated RhMYB108,an R2R3-MYB transcription factor,which is highly expressed in senescing petals as well as in petals treated with exogenous ethylene and JA.Applications of exogenous ethylene and JA markedly accelerated petal senescence,while the process was delayed in response to applications of 1-MCP,an ethylene action inhibitor.In addition,silencing of RhMYB108 alter the expression of SAGs such as RhNAC029,RhNAC053,RhNAC092,RhSAG12,and RhSAG113,and finally block ethylene-and JA-induced petal senescence.Furthermore,RhMYB108 was identified to target the promoters of RhNAC053,RhNAC092,and RhSAG113.Our results reveal a model in which RhMYB108 functions as a receptor of ethylene and JA signals to modulate the onset of petal senescence by targeting and enhancing senescence-associated gene expression. | Shuai Zhang Qingcui Zhao Daxing Zeng Jiehua Xu Hougao Zhou Fenglan Wang Nan Ma Yonghong Li | 2019 | Horticulture Research2019，6，1： | 10 |
| 3 | A Genomic Variation Map Provides Insights into the Genetic Basis of Spring Chinese Cabbage (Brassica rapa ssp.pekinensis)Selection显示文摘Chinese cabbage is the most consumed leafy crop in East Asian countries.However,premature bolting induced by continuous low temperatures severely decreases the yield and quality of the Chinese cabbage, and therefore restricts its planting season and geographic distribution.In the past 40years,spring Chinese cabbage with strong winterness has been selected to meet the market demand.Here,we report a genome variation map of Chinese cabbage generated from the resequencing data of 194 geographically diverse accessions of three ecotypes.In-depth analyses of the selection sweeps and genome-wide patterns revealed that spring Chinese cabbage was selected from a specific population of autumn Chinese cabbage around the area of Shandong peninsula in northern China.We identified 23 genomic loci that underwent intensive selection,and further demonstrated by gene expression and haplotype analyses that the incorporation of elite alleles of VERNALISATION INSENTIVE 3.1(BrVIN3.1)and FLOWER LOCUS C 1(BrFLC1)is a determinant genetic source of variation during selection.Moreover,we showed that the quantitative response of BrVIN3.1 to cold due to the sequence variations in the cis elements of the BrVlN3.1 promoter significantly contributes to bolting-time variation in Chinese cabbage.Collectively, our study provides valuable insights into the genetic basis of spring Chinese cabbage selection and will facilitate the breeding of bolting-resistant Varieties by molecular-marker-assisted selection,transgenic or gene editingapproaches. | Tongbing Su Weihong Wang Peirong Li Bin Zhang Pan Li Xiaoyun Xin Honghe Sun Yangjun Yu Deshuang Zhang Xiuyun Zhao Changlong Wen Gang Zhou Yuntong Wang Hongkun Zheng Shuancang Yu Fenglan Zhang | 2018 | Molecular Plant2018，11，11： | 6 |
| 4 | Identification of long noncoding RNAs involved in resistance to downy mildew in Chinese cabbage显示文摘Brassica downy mildew,a severe disease caused by Hyaloperonospora brassicae,can cause enormous economic losses in Chinese cabbage(Brassica rapa L.ssp.pekinensis)production.Although some research has been reported recently concerning the underlying resistance to this disease,no studies have identified or characterized long noncoding RNAs involved in this defense response.In this study,using high-throughput RNA sequencing,we analyzed the disease-responding mRNAs and long noncoding RNAs in two resistant lines(T12–19 and 12–85)and one susceptible line(91–112).Clustering and Gene Ontology analysis of differentially expressed genes(DEGs)showed that more DEGs were involved in the defense response in the two resistant lines than in the susceptible line.Different expression patterns and proposed functions of differentially expressed long noncoding RNAs among T12–19,12–85,and 91–112 indicated that each has a distinct disease response mechanism.There were significantly more cis-and trans-functional long noncoding RNAs in the resistant lines than in the susceptible line,and the genes regulated by these RNAs mostly participated in the disease defense response.Furthermore,we identified a candidate resistance-related long noncoding RNA,MSTRG.19915,which is a long noncoding natural antisense transcript of a MAPK gene,BrMAPK15.Via an agroinfiltration-mediated transient overexpression system and virus-induced gene silencing technology,BrMAPK15 was indicated to have a greater ability to defend against pathogens.MSTRG.19915-silenced seedlings showed enhanced resistance to downy mildew,probably because of the upregulated expression of BrMAPK15.This research identified and characterized long noncoding RNAs involved in resistance to downy mildew,laying a foundation for future in-depth studies of disease resistance mechanisms in Chinese cabbage. | Bin Zhang Tongbing Su Peirong Li Xiaoyun Xin Yunyun Cao Weihong Wang Xiuyun Zhao Deshuang Zhang Yangjun Yu Dayong Li Shuancang Yu Fenglan Zhang | 2021 | Horticulture Research2021，8，1： | 3 |
| 5 | Genome-wide analysis of changes in miRNA and target gene expression reveals key roles in heterosis for chinese cabbage biomass显示文摘Heterosis is a complex phenomenon in which hybrids show better phenotypic characteristics than their parents do.Chinese cabbage(Brassica rapa L.spp.pekinensis)is a popular leafy crop species,hybrids of which are widely used in commercial production;however,the molecular basis of heterosis for biomass of Chinese cabbage is poorly understood.We characterized heterosis in a Chinese cabbage hybrid cultivar and its parental lines from the seedling stage to the heading stage;marked heterosis of leaf weight and biomass yield were observed.Small RNA sequencing revealed 63 and 50 differentially expressed microRNAs(DEMs)at the seedling and early-heading stages,respectively.The expression levels ofthe majority of miRNA clusters in the hybrid were lower than the mid-parent values(MPVs).Using degradome sequencing,we identi fied 1,819 miRNA target genes.Gene ontology(GO)analyses demonstrated that the target genes ofthe MPV-DEMs and low parental expression level dominance(ELD)miRNAs were signi ficantly enriched in leaf morphogenesis,leaf development,and leaf shaping.Transcriptome analysis revealed that the expression levels of photosynthesis and chlorophyll synthesis-related MPV-DEGs(differentially expressed genes)were signi ficantly different in the F_(1) hybrid compared to the parental lines,resulting in increased photosynthesis capacity and chlorophyll content in the former.Furthermore,expression of genes known to regulate leaf development was also observed at the seedling stage.Arabidopsis plants overexpressing BrGRF4.2 and bra-miR396 presented increased and decreased leaf sizes,respectively.These results provide new insight into the regulation of target genes and miRNA expression patterns in leaf size and heterosis for biomass of B.rapa. | Peirong Li Tongbing Su Deshuang Zhang Weihong Wang Xiaoyun Xin Yangjun Yu Xiuyun Zhao Shuancang Yu Fenglan Zhang | 2021 | Horticulture Research2021，8，1： | 1 |
| 6 | Technological ehanllenges for CO2 EOR in China显示文摘 | Yue Xiang-an Zhao Renbao Zhao Fenglan | 2007 | Science Paper Online2007，2，7： | 1 |
| 7 | Author Correction: RhMYB108, an R2R3-MYB transcription factor, is involved in ethylene- and JA-induced petal senescence in rose plants显示文摘After publication of our article[1],we became aware that there were errors in Fig.7b,namely the negative control of pLacZi+pJG4-5-RhMYB108(2nd row,panel 1).The error does not affect the result,discussion or conclusion in the article.The correct version of Figure is shown below.We apologise to the journal and to readers for this error. | Shuai Zhang Qingcui Zhao Daxing Zeng Jiehua Xu Hougao Zhou Fenglan Wang Nan Ma Yonghong Li | 2019 | Horticulture Research2019，6，1： | 1 |
| 8 | Genetic mapping and localization of a major QTL for seedling resistance to downy mildew in Chinese cabbage (Brassica rapa ssp. pekinensis)显示文摘 | Shuancang Yu Fenglan Zhang Renbo Yu Yanmin Zou Jiani Qi Xiuyun Zhao Yangjun Yu Deshuang Zhang Li Li | 2009 | Molecular Breeding2009，，4： | 1 |
| 9 | DEVELOPMENT OF SENSOR NEEDLES FORCHINESE TRADITIONAL MEDICINE显示文摘Development of sensor needles for chinese traditional medicine is a long term re-search project in our university. Now, temperature. PH, PO2 and potential sensor needles havebeen developed. These new elements are derived from the combination between a sensor and an ac-tuator. The design principle, functional materials, fabrication technic, calibration method and clin-ical application of these needles are reported in this paper. | Shu Ren Tongpin Li Fenglan Yu Ersheng Kong Jie Shen Qi Jiang Hanan Liu Rongzhen Zhao, Tonji Medical University,Wuhan 430030,P. R. China | 1993 | Chinese Journal of Biomedical Engineering(English Edition)1993，2，1： | 1 |
| 10 | Surface properties of walnut protein from AOT reverse micelles 显示文摘 | Fenglan Liu Xianehang Wang Xiaoyan Zhao | 2014 | Int J Food Sci Technol2014，49，2： | 1 |
| 11 | Selenium Differentially Regulates Flavonoid Accumulation and Antioxidant Capacities in Sprouts of Twenty Diverse Mungbean(Vigna radiata(L.)Wilczek)Genotypes显示文摘Seed germination with selenium(Se)is promising for producing Se-biofortified foods.Mungbean(Vigna radiata(L.)Wilczek)sprout is freshly eaten as a salad dressed with sauce,making it superior for Se biofortification.Since the Se safety range for the human body is extremely narrow,it is imperative to evaluate the genotypic responses of mungbean sprouts to Se.This study evaluated the Se enrichment capacity and interaction withflavonoids and antioxidant systems in sprouts of 20 mungbean germplasms.Selenium treatment was done by immersing mung-bean seeds in 20μM sodium selenite solution for 8 h.Afterward,the biomass,Se amounts,flavonoid(particularly vitexin and isovitexin)contents,antioxidant capacity,and key biosynthetic gene expressions were measured.Sprout Se content was 2.0-7.0μg g^(-1) DW among the 20 mungbean germplasms.Selenium treatment differentially affected the biomass,totalflavonoid,vitexin,isovitexin,antioxidant enzyme activities,and antioxidant capacities of the mungbean germplasms.Eight germplasms showed increased biomass(p<0.05),the highest increasing by 127%,but 13 did not phenotypically respond to Se treatment.Seven and six germplasms showed varied levels of vitexin and isovitexin increment after Se treatment,the highest measuring 2.67-and 2.87-folds for vitexin and isovitexin,respectively.Two mungbeanflavonoid biosynthesis genes,chalcone synthase(VrCHS)and chalcone isomerase(VrCHI)were significantly up-regulated in the germplasms with increased vitexin and isovitexin levels(p<0.05).Moreover,Se enrichment capacity was significantly correlated with the vitexin,isovitexin,and antiox-idant capacities.In conclusion,mungbean sprouts could be a useful Se-biofortified food,but the Se enrichment capacity and nutritional response must be determined for each germplasm before commercialization. | Fenglan Zhao Jizhi Jin Meng Yang Franklin Eduardo Melo Santiago Jianping Xue Li Xu Yongbo Duan | 2024 | Phyton-International Journal of Experimental Botany2024，93，3： | 0 |
| 12 | Effects of Distiller's Grain Substrate with Acid Regulation on the Growth of Tobacco Seedlings in Floating Breeding System显示文摘In order to reduce the use of peat resources and realize the sustainable development of tobacco,the pH of distiller's grain substrate was adjusted with humic acid,and the effects of various distiller's grain substrates on the growth of tobacco seedlings were studied to find out the method of cultivating high-quality tobacco seedlings by using distiller's grain substrate. The results showed that when 60% of humic acid was added to the distiller's grain substrate made from coarse distiller's grains,fine distiller's grains,perlite and vermiculite,both the pH and electrical conductivity decreased significantly and were similar to that of the conventional substrate. Moreover,the emergence rate of tobacco seedlings,the rate of strong tobacco seedlings,leaf number,stem height,root growth and quality of tobacco seedlings were good. | Sheng JIANG Sang HU Fei XIONG Yonghui ZHANG Chun XIA Zilin LI Yongyu ZHAO Guangming TANG Fenglan QIN Qixin GU Jinchao ZHAO Junfeng XIN Fuzhao NIAN | 2018 | Agricultural Biotechnology2018，7，4： | 0 |
| 13 | Silencing of the Nonspecific Phospholipase C6(NPC6)Gene Induces Ricinoleic Acid Accumulation in Castor Seeds显示文摘Castor,scientifically known as Ricinus communis L.,is among the top ten oil crops globally.It is considered a renewable resource and is commonly referred to as‘green oil’.Castor seeds contain castor oil as their main component,which is predominantly composed of ricinoleic acid.This study utilized RNAi technology to silence the NPC6 gene in NO.2129 castor,resulting in the creation of mutant plants L1 and L2.The weight of 100 dry seed kernels from L1 and L2 exceeds that from NO.2129.The crude fat and ricinoleic acid levels of L1 and L2 were higher than those of NO.2129 at various developmental stages.In the proteomics analysis of 60-day-old castor seeds,a total of 21 differentially expressed proteins were identified,out of which 19 were successfully recognized.Eleven of the differentially expressed proteins identified were legumins,which play a crucial role in nutrient storage within the seed.Silencing the NPC6 gene results in the accumulation of ricinoleic acid in castor seeds.The findings of this study not only enhance our knowledge of NPC6’s role in regulating castor seed oil synthesis but also offer fresh perspectives for investigating oil synthesis and accumulation in other plant species. | Yong Zhao Lili Li Rui Luo Mu Peng Tongtong Jiang Mingda Yin Yanpeng Wen Zihan Wang Fenglan Huang Fanjuan Meng | 2023 | Phyton-International Journal of Experimental Botany2023，92，12： | 0 |
| 14 | Proteomic Analyses of Three Inflorescence Styles of Castor(Ricinus communis L.)at Different Developmental Stages显示文摘Castor(Ricinus communis L.)is one of ten oil crops in the world and has complex inflorescence styles.Generally,castor has three inflorescence types:single female inflorescence(SiFF),standard female inflorescence(StFF)and bisexual inflorescence(BF).StFF is realized as a restorer line and as a maintainer line,which was applied to castor hybrid breeding.However,the developmental mechanism of the three inflorescences is not clear.Therefore,we used proteomic techniques to analyze different inflorescence styles.A total of 72 diferentially abundant protein species(DAPs)were detected.These DAPs are primarily involved in carbon and energy metabolism and carbon fixation in the photosynthetic organism pathway.The results showed that DAPs are involved in photosynthesis to control the distribution of imported carbohydrates and exported photoassimilates and thus affect the inflorescence development of castor.In addition,these DAPs are also involved in cysteine and methionine metabolism.Quantitative real-time PCR(qRT-PCR)results demonstrated that the proteomics data collected in this study were reliable.Our findings indicate that the carbon cycle and amino acid metabolism influence the inflorescence development of castor. | Xue Lei Yong Zhao Rui Luo Mingda Yin Yanpeng Wen Zhiyan Wang Xuemei Hu Fenglan Huang | 2023 | Phyton-International Journal of Experimental Botany2023，92，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员