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| 1 | Polyphenolic Compound and the Degree of Browning in Processing Apple Varieties显示文摘Polyphenolic compound in processing apple (Malus domestica Borkh.) varieties and the relationship between polyphenol content and enzymatic browning were studied to provide reference for raw material selection and processing method optimization. The content of polyphenol compound in 10 processing apple varieties (4 cider and 6 juice varieties) were analyzed using the Folin-Ciocalteu method and HPLC. The degree of browning and the activities of polyphenol oxidase were also studied. The content and proportion of the polyphenol varied depending on the variety. Bitter varieties globally showed a higher polyphenol concentration than sweet or acid varieties. Proanthocyanidins, chlorogenic acid, (+)-catechin, (-)-epicatechin were high-concentrated polyphenols in apple fruits. Phloridzin, the unique polyphenol of apple, was abundant in the bitter variety Frequin rouge fruit. Total polyphenols, proanthocyanidins, (+)-catechin, and phloridzin had higher correlations with browning. The correlation was low between chlorogenic acid and browning. The polyphenolic profiles were correlated with the apple types. Cider apples contained more polyphenol than juice apple varieties. The content of flavan-3-ol has a close relationship with fruit browning. | SONG Ye YAO Yu-xin ZHAI Heng DU Yuan-peng CHEN Feng WEI Shu-wei | 2007 | Agricultural Sciences in China2007,6,5: | 17 |
| 2 | Analysis of Genetic Diversity of Processing Apple Varieties显示文摘Genetic diversity of 18 processing apple varieties and two fresh varieties were evaluated using 12 simple sequence repeats (SSR) primer pairs previously identified in Malus domestica Borkh. A total of 87 alleles in 10 loci were detected using 10 polymorphic SSR markers selected within the range of 5-14 alleles per locus. All the 20 varieties could be distinguished using two primer pairs and they were divided into four groups using cluster analysis. The genetic similarity (GS) of groups analyzed using cluster analysis varied from 0.14 to 0.83. High acid variety Avrolles separated from other varieties with GS less than 0.42. The second group contained Longfeng and Dolgo from Northeast of China, the inherited genes of Chinese crab apple. The five cider varieties with high tannin contents, namely, Dabinette, Frequin rouge, Kermerrien, M.Menard, and D.Coetligne were clustered into the third group. The fourth group was mainly composed of 12 juice and fresh varieties. Principal coordinate analysis (PCO) also divided all the varieties into four groups. Juice and fresh apple varieties, Longfeng and Dolgo were clustered together, respectively, using both the analyses. Both the analyses showed there was much difference between cider and juice varieties, cider and fresh varieties, as well as Chinese crab apple and western European crab apple, whereas juice varieties and fresh varieties had a similar genetic background. The genetic diversity and differentiation could be sufficiently reflected by combining the two analytical methods. | SONG Ye ZHAI Heng YAO Yu-xin LI Ming DU Yuan-peng | 2006 | Agricultural Sciences in China2006,5,10: | 10 |
| 3 | The Relation Between Endogenous Hormones and Late-Germination in Buds of Avrolles Apple显示文摘In order to provide the physiological bases for selecting late-germination cultivars that can avoid late frost damage, the very late-germination variety Avrolles (Malus domestica) was used to study the relation between the dynamic changes and balance of endogenous hormones and germination time. The concentrations of endogenous GA3, ABA, IAA, and ZR were determined in buds of Avrolles and Judeline (Malus domestica) from dormancy releasing to germination by capillary electrophoresis. The dynamic changes of endogenous hormones concentration in buds of Avrolles and Judeline were similar; but the magnitude and time of the change were significantly different between the two varieties, especially for GA3. GA3 concentration increased with dormancy releasing, then decreased, and increased again before germination in the two varieties. GA3 concentration in Avrolles was 1.72 times that in Judeline at the first peak, the gap increased to 2.22 times at germination. ZR concentration exhibited a continuous increase trend, but it decreased sharply before germination. ZR accumulation in Avrolles took 36 days longer than in Judeline, the peak value was 44% higher than in Judeline. Before germination, ZR concentration in Avrolles was 2.12 times that in Judeline. The differences between IAA and ABA concentration were relatively small in the two varieties, while the ratios of GA3/ABA and (GA3 + IAA + ZR)/ABA in Avrolles were 2.08 and 1.58 times those in Judeline, respectively. The germination of apple bud was regulated by the endogenous hormones. For the late-germination apple Avrolles, its germination requires higher concentration of GA3 and ZR, which leads to the high ratios of GA3/ABA and (GA3 + IAA + ZR)/ABA. | QIN Dong WANG Jin-zheng GUO Jian-min ZHAI Heng | 2009 | Agricultural Sciences in China2009,8,5: | 10 |
| 4 | Functional characterization of WRKY46 in grape and its putative role in the interaction between grape and phylloxera (Daktulosphaira vitifoliae)显示文摘WRKY transcription factors are involved in defense responses caused by biotic stresses.Phylloxera(Daktulosphaira vitifoliae Fitch),a pest widespread in viticulture,elicits transcriptional reprogramming of plant defense-associated components,such as regulons related to WRKYs and salicylic acid(SA)signaling.In this study,we characterized WRKY46,a WRKY transcription factor responsible for phylloxera attack,and revealed the molecular mechanism for WRKY-mediated defense responses to phylloxera.qRT-PCR and GUS staining analyses revealed that WRKY46 is induced in response to phylloxera damage and mechanical wounding.VvWRKY46 is a nuclear-localized transcription factor that activates its downstream target VvCHIB by direct protein–DNA interaction.Regulons involved in the SA-mediated defense response were regulated during incompatible interactions between“1103 Paulsen”rootstock and phylloxera.In addition,WRKY46 exhibited a higher transcript abundance in“1103 Paulsen”than in“Crimson Seedless”,regardless of whether the plants were infected with phylloxera.Furthermore,the enhanced expression of VvWRKY46 significantly attenuated phylloxera attack and delayed nymph development of composite grape plants.In summary,we demonstrated that WRKY46 plays a role in the SA-mediated defense-regulatory network by directly binding to the downstream structural gene VvCHIB.The phylloxera-responsive gene WRKY46 was identified,which could improve the understanding of the basic mechanism of grapevine in response to phylloxera. | Feng-Pan Wang Pan-Pan Zhao Lei Zhang Heng Zhai Yuan-Peng Du | 2019 | Horticulture Research2019,6,1: | 6 |
| 5 | Curcumin inhibits the replication of enterovirus 71 in vitro显示文摘Human enterovirus 71(EV71)is the main causative pathogen of hand,foot,and mouth disease(HFMD)in children.The epidemic of HFMD has been a public health problem in Asia-Pacific region for decades,and no vaccine and effective antiviral medicine are available.Curcumin has been used as a traditional medicine for centuries to treat a diversity of disorders including viral infections.In this study,we demonstrated that curcumin showed potent antiviral effect again EV71.In Vero cells infected with EV71,the addition of curcumin significantly suppressed the synthesis of viral RNA,the expression of viral protein,and the overall production of viral progeny.Similar with the previous reports,curcumin reduced the production of ROS induced by viral infection.However,the antioxidant property of curcumin did not contribute to its antiviral activity,since N-acetyl-L-cysteine,the potent antioxidant failed to suppress viral replication.This study also showed that extracellular signal-regulated kinase(ERK)was activated by either viral infection or curcumin treatment,but the activated ERK did not interfere with the antiviral effect of curcumin,indicating ERK is not involved in the antiviral mechanism of curcumin.Unlike the previous reports that curcumin inhibited protein degradation through ubiquitin–proteasome system(UPS),we found that curcumin had no impact on UPS in control cells.However,curcumin did reduce the activity of proteasomes which was increased by viral infection.In addition,the accumulation of the short-lived proteins,p53 and p21,was increased by the treatment of curcumin in EV71-infected cells.We further probed the antiviral mechanism of curcumin by examining the expression of GBF1 and PI4KB,both of which are required for the formation of viral replication complex.We found that curcumin significantly reduced the level of both proteins.Moreover,the decreased expression of either GBF1 or PI4KB by the application of siRNAs was sufficient to suppress viral replication.We also demonstrated that curcumin showed anti-apoptotic activity at the early stage of viral infection.The results of this study provide solid evidence that curcumin has potent anti-EV71 activity.Whether or not the down-regulated GBF1 and PI4KB by curcumin contribute to its antiviral effect needs further studies. | Ying Qin Lexun Lin Yang Chen Shuo Wu Xiaoning Si Heng Wu Xia Zhai Yan Wang Lei Tong Bo Pan Xiaoyan Zhong Tianying Wang Wenran Zhao Zhaohua Zhong | 2014 | Acta Pharmaceutica Sinica B2014,4,4: | 5 |
| 6 | Melatonin Treatment Enhances the Polyphenol Content and Antioxidant Capacity of Red Wine显示文摘Melatonin and polyphenols are strong antioxidants, and melatonin is also a potential plant growth regulator. The role of melatonin in regulating polyphenol metabolism is unclear. This study assessed the primary impacts of exogenous melatonin treatment on phenolics accumulation and antioxidant capacity of the 'Moldova' wine. It was found that two times of 100 μmol·L^(-1) melatonin treatment clearly enhanced the endogenous melatonin content of ripened berries and wine. Further experiments indicated that melatonin treatment significantly increased the contents of total phenols, flavonoids and anthocyanins in wine. Additionally, the contents of most of the 20 detected individual phenolic compounds were significantly enhanced by melatonin treatment in wine. Particularly, the content of two non-flavonoid phenolic compounds(syringic and coumaric acid) and four anthocyanin compounds [Mv-3-Glu, Mv-3-(6-Coum)Glu-5-Glu, Dp-3-(6-AC)Glu and Dp-3-(6-Coum)Glu-5-Glu]were largely increased by melatonin treatment. In contrast, only Pn-3-(6-Coum)Glu was significantly reduced. Moreover, melatonin treatment significantly enhanced the antioxidant capacity of wine indicated by DPPH and FRAP assays. In summary, melatonin treatment enhanced the polyphenol content and antioxidant capacity of wine. | XU Lili YUE Qianyu BIAN Feng'e ZHAI Heng YAO Yuxin | 2018 | Horticultural Plant Journal2018,4,4: | 4 |
| 7 | Effects of 1-MCP on Ethylene Synthesis and Metabolismof Apple During Storage显示文摘Red Fuji apple (Malus domestica Borkh var. Red Fuji) fruits were used to study the effect of 1-MCP on ethylene biosynthesis metabolism during storage. The results showed that 1-MCP maintained the firmness and inhibited the respiration rate, LOX activity and ethylene production rate of fruits. Further study indicated that 1-MCP inhibited ACS (ACC synthase) activity from the 15th day, increased ACC accumulation,and delayed the appearance of ACO (ACC oxidase) activity peak. The increase of protein kinase activity was also inhibited by 1-MCP during fruit ethylene climacteric time. | LI Fu-jun, ZHAI Heng, YANG Hong-qiang, ZHANG Xin-hua, SHU Huai-rui and ZHOU Jie(Horticultural College , Shandong Agricultural University, Tai’an 271018 , P.R. China) | 2003 | Agricultural Sciences in China2003,2,11: | 4 |
| 8 | Preparation of polypyrrole/polyvinylalcohol(PPy/PVA)composite foam electrode material显示文摘Polypyrrole/polyvinylalcohol(PPy/PVA) foam was prepared by direct foam polymerization in water and then it was coated on the indium-tin oxide transparent conductive glass(ITO) to form conventional three-electrode cell.FTIR and UV-vis spectra were adopted to characterize the molecular structure and the absorption spectra of foam material,respectively.The porous structure of PPy/PVA foams and their photoelectric conversion behaviors were studied.The dimension of the pores is bigger than 100μm in diameter.Compared with the smooth film,the V_(oc) and I_(sc) of the foam film enhanced by 1.58-fold and 5.59-fold,respectively. | Xin Yi Wang Li Ping Heng Nai Liang Yang Qiang Xie Jin Zhai | 2010 | Chinese Chemical Letters2010,21,7: | 3 |
| 9 | Numerical modeling and simulation of temperature distribution uncertainty subject to ferromagnetic thermoseeds hyperthermia显示文摘In the process of ferromagnetic thermoseeds mediated magnetic induction hyperthermia,the temperature distribution in the tumor region is a key factor to determine the therapy effect.During the preoperative treatment planning,discrepancies of the treatment parameters may lead to the temperature distribution uncertainty within the target area.Inaccurate prediction of temperature distribution may induce the treatment failure;therefore,it would be significant to investigate the uncertainty of tissue temperature prediction caused by the disturbance of calculation parameters.In this paper,3D temperature field and necrosis zone of tissues in ferromagnetic thermoseeds hyperthermia are simulated by the finite volume algorithm,and effects of parameter uncertainties on the temperature distribution are revealed.Results show that selecting appropriate magnetic field parameters for treatment is a priority to guarantee therapeutic effect.Thermoseed properties and blood perfusion rate are the obvious disturbing terms for temperature distribution,while the metabolic heat of bio-tissues can often be ignored within limits.Our investigation is of importance for guiding reasonable and optimal preoperative treatment planning. | Zi-Han Zhuo Jie Wang Wei-Ming Zhai Heng Wang Jin-Tian Tang | 2014 | Chinese Science Bulletin2014,59,12: | 2 |
| 10 | Rice GLUTATHIONE PEROXIDASE1-mediated oxidation of bZIP68 positively regulates ABA-independent osmotic stress signaling显示文摘Osmotic stress caused by drought and high salinity is a significant environmental threat that limits plant growth and agricultural yield. Redox regulation plays an important role in plant stress responses, but the mechanisms by which plants perceive and transduce redox signals are still underexplored. Here, we report a critical function for the thiol peroxidase GPX1 in osmotic stress response in rice, where it serves as a redox sensor and transducer. GPX1 is quickly oxidized upon exposure to osmotic stress and forms an intramolecular disulfide bond, which is required for the activation of bZIP68, a VRE-like basic leucine zipper (bZIP) transcription factor involved in the ABA-independent osmotic stress response pathway. The disulfide exchange between GPX1 and bZIP68 induces homo-tetramerization of bZIP68 and thus positively regulates osmotic stress response by regulating osmotic-responsive gene expression. Furthermore, we discovered that the nuclear translocation of GPX1 is regulated by its acetylation under osmotic stress. Taken together, our findings not only uncover the redox regulation of the GPX1-bZIP68 module during osmotic stress but also highlight the coordination of protein acetylation and redox signaling in plant osmotic stress responses. | Heng Zhou Feng Zhang Fengchao Zhai Ye Su Ying Zhou Zhenglin Ge Priyadarshini Tilak Jürgen Eirich Iris Finkemeier Ling Fu Zongmin Li Jing Yang Wenbiao Shen Xingxing Yuan Yanjie Xie | 2022 | Molecular Plant2022,15,4: | 2 |
| 11 | Dynamic Changes in the Calcium Content of Several Apple Cultivars During the Growing Season显示文摘Dynamic changes in calcium content were investigated in eight apple cultivars. The results showed that the calcium concentration in leaves and shoots increased with fruit development. The cultivars displayed only a small difference in the calcium concentration during the early stage of development, the difference became very significant at the late stage of development, especially in shoots. In shoots, for example, calcium content was highest in Starkrimson (19 638.6 mg kg-1) and lowest in Fuji (8 751.3 mg kg-1). Calcium concentration was highest in young fruits and was found to decrease with the growth of fruit, and was characterized by a dramatic drop at the rapid expansion stage. There was a significant difference among cultivars. Young Starkrimson fruits contained the highest calcium concentration of 506.52 mg kg-1 among cultivars tested, followed by Pink Lady and Fuji. The calcium concentration in mature fruits from high to low is as follows: Starkrimson, Sansa, Pink Lady, Senshu, Gala, Fuji, Red General and New Century. In this study, it was found that eight cultivars continuously assimilated calcium during the whole growing season, especially at the young stage when fruit took up 35-46% of total calcium. The calcium content in fruitlets was low in all cultivars; in the expansion stage, there was rapid absorption of about 30% of total calcium, whereas in the ripening fruit, content of calcium was reduced. The calcium accumulation increased with fruit growth in stalk, similar to that in fruit. | ZHENG Wei-wei YOU Chun-xiang DU Zhong-jun ZHAI Heng | 2006 | Agricultural Sciences in China2006,5,12: | 2 |
| 12 | Effects of Partial Rootzone Drying on the Growth of Vitis vinifera cv.Malvasia Grafted on Different Rootstocks显示文摘To lay a biological foundation for rootstocks and alternate irrigation (AI) popularization, the effects of partial rootzone drying (PRD) on the growth of the grapevine Malvasia grafted on different rootstocks were investigated. Biological effects of 1/2 divided root irrigation on three combinations, i.e., Malvasia/420A, Malvasia/3309C, and Malvasia/110R, were studied by wood-boxed plants. All the plants were separated into three groups for different irrigation strategies. Mass growth of new root in alternate-irrigated plants was remarkably promoted by about 7.8-22.2% higher than the well- watered ones. However, new shoot growth, especially the internode was reduced by alternate irrigation. The average root-shoot ratio of all the three combinations was increased from 1.1 to 1.46. New root growth and internode length were decreased by fixed partial rootzone irrigation (FI) at different amount, M/3309C at 37.9 and 36.9%, M/110R at 18.4 and 22.5%, respectively. Total biomass of all the three combinations under FI decreased at the rate of 19.2-34.3% compared with well-watered ones. Water stress adaptation of grapevine mainly depends on rootstock. 110R is more efficient than 3309C and 420A in water stress adaptation. PRD-AI benefited root growth, thus improved the drought-resistant ability of grapevine. | QI Wei GUAN Xue-qiang LI En-mao ZHAI Heng WANG Xiao-fang DU Yuan-peng | 2007 | Agricultural Sciences in China2007,6,5: | 2 |
| 13 | Solvent fuming dual-responsive switching of both wettability and solid-state luminescence in silole film显示文摘 | Heng L P Dong Y Q Zhai J | 2008 | Langmuir2008,24,: | 1 |
| 14 | Multiple models and neural networks based decoupling control of ball mill coal-pulverizing systems显示文摘 | Tianyou Chai Lianfei Zhai Heng Yue | 2011 | Journal of Process Control2011,21,3: | 1 |
| 15 | Multiple models and neural networks based decoupling control of ball mill coal-pulverizing systems显示文摘 | Tianyou Chai Lianfei Zhai Heng Yue | 2010 | Journal of Process Control2010,,3: | 1 |
| 16 | Bio-inspired multi-scale struc- tures in dye-sensitized solar cell显示文摘 | JIN L ZHAI J HENG L | 2009 | Journal of Photochemistry and Photobiology C: Photochemistry Reviews2009,10,4: | 1 |
| 17 | Fabrication of Hexaphenylsilole Nanowires and Their Morphology-Tunable Photoluminescence 显示文摘 | Heng L P Zhai J Qin A J | 2007 | ChemPhysChem2007,8,10: | 1 |
| 18 | Multiple models andneural networks based decoupling control of ball mill coal - pulveri-zing systems显示文摘 | Chai Tian - you Zhai Li an - fei Yue Heng | 2011 | Journal of Process Control2011,21,: | 1 |
| 19 | Multiple Models and Neural Networks Based Decoupling Control of Ball Mill Coal-pulverizing Systems显示文摘 | CHAI T Y ZHAI L F HENG Y | 2011 | Journal of Process Control2011,21,03: | 1 |
| 20 | Multiple models and neural networks based decoupling control of ball mill coal-pulverizing systems显示文摘 | Tianyou Chai Lianfei Zhai Heng Yue | 2010 | Journal of Process Control2010,,3: | 1 |