|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Adenovirus-mediated gene delivery:Potential applications for gene and cell-based therapies in the new era of personalized medicine显示文摘With rapid advances in understanding molecular pathogenesis of human diseases in the era of genome sciences and systems biology,it is anticipated that increasing numbers of therapeutic genes or targets will become available for targeted therapies.Despite numerous setbacks,efficacious gene and/or cell-based therapies still hold the great promise to revolutionize the clinical management of human diseases.It is wildly recognized that poor gene delivery is the limiting factor for most in vivo gene therapies.There has been a long-lasting interest in using viral vectors,especially adenoviral vectors,to deliver therapeutic genes for the past two decades.Among all currently available viral vectors,adenovirus is the most efficient gene delivery system in a broad range of cell and tissue types.The applications of adenoviral vectors in gene delivery have greatly increased in number and efficiency since their initial development.In fact,among over 2000 gene therapy clinical trials approved worldwide since 1989,a significant portion of the trials have utilized adenoviral vectors.This review aims to provide a comprehensive overview on the characteristics of adenoviral vectors,including adenoviral biology,approaches to engineering adenoviral vectors,and their applications in clinical and preclinical studies with an emphasis in the areas of cancer treatment,vaccination and regenerative medicine.Current challenges and future directions regarding the use of adenoviral vectors are also discussed.It is expected that the continued improvements in adenoviral vectors should provide great opportunities for cell and gene therapies to live up to its enormous potential in personalized medicine. | Cody S.Lee Elliot S.Bishop Ruyi Zhang Xinyi Yu Evan M.Farina Shujuan Yan Chen Zhao Zongyue Zeng Yi Shu Xingye Wu Jiayan Lei Yasha Li Wenwen Zhang Chao Yang Ke Wu Ying Wu Sherwin Ho Aravind Athiviraham Michael J.Lee Jennifer Moriatis Wolf Russell R.Reid Tong-Chuan He | 2017 | Genes & Diseases2017，4，2： | 19 |
| 2 | Biosynthetic pathway of terpenoid indole alkaloids in Catharanthus roseus显示文摘Catharanthus roseus 是最广泛地调查的药用的植物之一,它能生产超过 130 碱,包括强大的 antitumor 药 vinblastine 和长春新碱。这里,我们在 C 在 terpenoid 吲哚碱(TIA ) 的 biosynthetic 小径考察最近的进展。涉及这条小径的相应的酶的 roseus,和鉴定和描述。Strictosidine 是在不同 TIA 的生合成的中央中介,它被 secologanin 和 tryptamine 的冷凝作用形成。Secologanin 从 terpenoid (isoprenoid ) 被导出 biosynthetic 小径,当 tryptamine 从吲哚 biosynthetic 被导出时小径。然后各种各样的特定的结束产品被不同线路在下游的过程期间生产。尽管许多基因和相应的酶在这条小径被描绘了,我们整个 TIA biosynthetic 小径上的知识仍然保持大部分未知最新。TIA biosynthetic 小径的完整的说明是一个重要前提在药用的植物理解 TIA 生合成的规定并且由合成生物技术生产珍贵 TIA。 | Xiaoxuan Zhu Xinyi Zeng Chao Sun Shilin Chen | 2014 | Frontiers of Medicine2014，8，3： | 8 |
| 3 | Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes(imPODs)显示文摘Glomerular podocytes are highly specialized epithelial cells and play an essential role in establishing the selective permeability of the glomerular filtration barrier of kidney.Maintaining the viability and structural integrity of podocytes is critical to the clinical management of glomerular diseases,which requires a thorough understanding of podocyte cell biology.As mature podocytes lose proliferative capacity,a conditionally SV40 mutant tsA58-immortalized mouse podocyte line(designated as tsPC)was established from the Immortomouse over 20 years ago.However,the utility of the tsPC cells is hampered by the practical inconvenience of culturing these cells.In this study,we establish a user-friendly and reversibly-immortalized mouse podocyte line(designated as imPOD),on the basis of the tsPC cells by stably expressing the wildtype SV40 T-antigen,which is flanked with FRT sites.We show the imPOD cells exhibit long-term high proliferative activity,which can be effectively reversed by FLP recombinase.The imPOD cells express most podocyte-related markers,including WT-1,Nephrin,Tubulin and Vinculin,but not differentiation marker Synaptopodin.The imPOD cells do not form tumor-like masses in vivo.We further demonstrate that TGFb1 induces a podocyte injury-like response in the FLP-reverted imPOD cells by suppressing the expression of slit diaphragm-associated proteins P-Cadherin and ZO-1 and upregulating the expression of mesenchymal markers,a-SMA,Vimentin and Nestin,as well as fibrogenic factors CTGF and Col1a1.Collectively,our results strongly demonstrate that the newly engineered im-POD cells should be a valuable tool to study podocyte biology both under normal and under pathological conditions. | Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C.Haydon Hue H.Luu Tong-Chuan He Hua Gan | 2018 | Genes & Diseases2018，5，2： | 5 |
| 4 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 5 | Ginsenoside Rg1 Prevents Cogni- tive Impairment and Hippocampus Senescence in a Rat Model of D - Galactose - In- duced Aging 显示文摘 | Jiahong Zhu Xinyi Mu Jin Zeng | 2014 | PLOS ONE2014，9，6： | 1 |
| 6 | Af- forestation in China cools local land surface tempera ture显示文摘 | PENG Shushi PIAO Shilong ZENG Xinyi | 2014 | Proceedings of the National Academy of Sci- ences2014，111，8： | 1 |
| 7 | Application of Bayesian Regularized BP Neural Network Model for Trend Analysis, Acidity and Chemical Composition of Precipitation in North Carolina显示文摘 | Min Xu Guangming Zeng Xinyi Xu Guohe Huang Ru Jiang Wei Sun | 2006 | Water Air and Soil Pollution (-)2006，，1： | 1 |
| 8 | JaponiconeA induces apoptosis of bortezomib-sensitive and -resistant myeloma cells in vitro and in vivo by targeting IKKβ显示文摘Objective:Multiple myeloma(MM)remains incurable with high rates of relapse.New therapeutic drugs are therefore urgently needed to improve the prognosis.JaponiconeA(JA),a natural product isolated from Inula japonica Thunb,has shown good anti-MM potential.A comprehensive study should therefore be conducted to identify both the in vitro and in vivo mechanisms of the anti-MM effects of JA.Methods:CCK8 assays and flow cytometry were used to detect the proliferation,apoptosis,and cell cycle of MM cell lines when treated with JA.In vivo experiments were conducted using subcutaneous xenograft mouse models.We also identified possible targets and the mechanism of JA using RNA-seq and c-Map databases,and identified the specific targets of JA in bortezomib-sensitive and-resistant MM cell lines using CETSA,DARTS,and rescue experiments.Furthermore,JA and bortezomib were used separately or together to characterize their possible synergistic effects.Results:In vitro,JA inhibited proliferation,and induced apoptosis and G2/M phase arrest in MM cell lines,and selectively killed primary CD138+MM cells.In vivo,JA also demonstrated a strong anti-tumor effect with no observable toxicity.In addition,JA showed synergetic effects in combination with bortezomib,and enhanced the anti-tumor effect of bortezomib in bortezomibresistant cells.CETSA and DARTS confirmed direct binding of JA to NF-κB inhibitor kinase beta(IKKβ),and overexpression of IKKβor knockdown of IκBαpartially rescued the apoptosis induced by JA.Conclusions:JA exhibited strong anti-tumor effects in MM.It sensitized myeloma cells to bortezomib and overcame NF-κB-induced drug resistance by inhibiting IKKβ,providing a new treatment strategy for MM patients. | Zilu Zhang Chenjing Ye Jia Liu Wenbin Xu Chao Wu Qing Yu Xiaoguang Xu Xinyi Zeng Huizi Jin Yingli Wu Hua Yan | 2022 | Cancer Biology & Medicine2022，19，5： | 1 |
| 9 | Mechanism of cancer: Oncohistones in action显示文摘Oncohistones are histones with high-frequency point mutations that are associated with tumorigenesis.Although each histone variant is encoded by multiple genes, a single mutation in one allele of one gene seems to have a dominant effect over global histone H3 methylation level at the relevant amino acid residue. These oncohistones are highly tumor type specific. For example, H3K27M and H3G34V/R mutations occur only in pediatric brain cancers, whereas H3K36M and H3G34W/L have only been found in pediatric bone tumors. H1 mutations also seem to be exclusively linked to lymphomas. In this review, we discuss the occurrence, frequency and potential functional mechanisms of each oncohistone in tumorigenesis of its relevant cancer. We believe that further investigation into the mechanism regarding their tumor type specificity and cancer-related functions will shed new light on their application in cancer diagnosis and targeted therapy development. | Lei Qiu Xiaoyan Hu Qian Jing Xinyi Zeng Kui-Ming Chan Junhong Han | 2018 | Journal of Genetics and Genomics2018，45，5： | 1 |
| 10 | Ginsenoside Rgl prevents cognitive impairment and hippocampus senescence in a rat model of d-galactose-induced aging 显示文摘 | Jiahong Zhu Xinyi MU Jin Zeng | 2014 | PLoS 0ne2014，9，10： | 1 |
| 11 | Supercontinuum fiber laser-based coherent anti-Stokes Raman scattering microscopy for label-free chemical imaging显示文摘Coherent anti-Stokes Raman scattering(CA RS)microscopy can resolve the chemical compo-nents and distribution of living biological systems in a label-firee manner and is favored in several disciplines.Current CA RS microscopes typically use bulky,high-performance solid-state lasers,which are expensive and sensitive to environmental changes.With their relatively low cost and environmental sensitivity,supercontinum fiber(SF)lasers with a small footprint have found increasing use in biomedical applications.Upon these features,in this paper,we homebuilt a low-cost CARS microscope based on a SF laser module(scCA RS microscope).This SF laser module is specially customized by adding a time synchronized seed source channel to the SF laser to form a dual-channel output laser.The performance of the scCARS microscope is evaluated with dimethyl sulfoxide,whose results confirm a spatial resolution of better than 500nm and a detection sensitivity of millimolar concentrations.The dual-color imaging capability is further demonstrated by imaging different species of mixed microspheres.We finally explore the potential of our scCARS microscope by mapping lipid droplets in different cancer cells and corneal stromal lenses. | Nan Wang Jiaojiao Zhang Wangting Zhou Siting Liu Jing Li Xinyi Xu Qi Zeng Yong Li Shouping Zhu Xueli Chen | 2022 | Journal of Innovative Optical Health Sciences2022，15，4： | 0 |
| 12 | Coupling Analysis of Tourism Development and Rural Revitalization in Yannanfei Tea Field Resort of Guangdong Province显示文摘By deconstructing the dimensions of rural tourism and rural revitalization and constructing a coupled conceptual model,a coupled evaluation model is constructed.Yannanfei Tea Field Resort in Guangdong is taken as a research case for empirical analysis,to explore the coupled development trend of the two.The results show that①the tourism development of Guangdong Yannanfei Tea Field Resort has a good synergistic relationship with rural revitalization.The two complement and promote each other,and rural tourism has significantly promoted the development of rural revitalization.②The coupling development of tourism development and rural revitalization in Guangdong Yannanfei Tea Field Resort is divided into coupling imbalance period and coupling coordination period.The results indicate that the coordination between the two has not yet reached a level of good coordination and high-quality coordination.It is recommended that the resort should fully utilize the opportunity of rural revitalization strategy,increase investment in rural tourism,promote the transformation and upgrading of the resort,impel the integration of tea and tourism,and develop new business model of tea-tourism rural vacation. | CHEN Haiming LIU Jun ZENG Ziye FENG Jinhuan WU Xinyi WU Zhuohua CHEN Yiyu | 2023 | Journal of Landscape Research2023，15，6： | 0 |
| 13 | Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy显示文摘Chemoresistance has long been the bottleneck of ovarian cancer(OC)prognosis.It has been shown that mitochondria play a crucial role in cell response to chemotherapy and that dysregulated mitochondrial dynamics is intricately linked with diseases like OC,but the underlying mechanisms remain equivocal.Here,we demonstrate a new mechanism where CRL4^(CUL4A/DDB1)manipulates OC cell chemoresistance by regulating mitochondrial dynamics and mitophagy.CRL4^(CUL4A/DDB1)depletion enhanced mitochondrial fission by upregulating AMPKα^(Thr172)and MFF^(Ser172/Ser146)phosphorylation,which in turn recruited DRP1 to mitochondria.CRL4^(CUL4A/DDB1)loss stimulated mitophagy through the Parkin-PINK1 pathway to degrade the dysfunctional and fragmented mitochondria.Importantly,CRL4^(CUL4A/DDB1)loss inhibited OC cell proliferation,whereas inhibiting autophagy partially reversed this disruption.Our findings provide novel insight into the multifaceted function of the CRL4 E3 ubiquitin ligase complex in regulating mitochondrial fission,mitophagy,and OC chemoresistance.Disruption of CRL4^(CUL4A/DDB1)and mitophagy may be a promising therapeutic strategy to overcome chemoresistance in OC. | Yang Meng Lei Qiu Xinyi Zeng Xiaoyan Hu Yaguang Zhang Xiaowen Wan Xiaobing Mao Jian Wu Yongfeng Xu Qunli Xiong Zhixin Chen Bo Zhang Junhong Han | 2023 | Signal Transduction and Targeted Therapy2023，8，1： | 0 |
| 14 | Highly sensitive colorimetric detection of NH3 based on Au@Ag@AgCl core-shell nanoparticles显示文摘Abstract:As an important component of the atmosphere,ammonia(NH_(3))plays a very important role in maintaining the balance of environment.However,it is also one of the most toxic gases that can cause damage to the human respiratory system and mucous membranes even at low concentrations.As such,development of highly sensitive and selective NH_(3)sensors is of high significance for environmental monitoring and health maintenance.Herein,we have synthesized Au@Ag@Ag Cl core-shell nanoparticles(NPs)by oxidative etching and precipitating Au@Ag core-shell NPs using FeCl3 and further used them as optical probes for the colorimetric detection of NH_(3).The sensing mechanism is based on the fact that the etching of NH_(3)on AgCl and Ag shell leads to the variations of ingredients and core-to-shell ratio of the Au@Ag@AgCl NPs,thereby inducing noticeable spectral and color changes.By replacing the outmost layer of Ag with AgCl,not only is the stability of the sensor against oxygen significantly enhanced,but also is the sensitivity of the method improved.The method exhibits good linear relationship for the detection of NH_(3)from 0 to 5000 mmol/L with the limit of detection of 6.4 mmol/L.This method was successfully applied to the detection of simulated air polluted by NH_(3),indicating its practical applicability for environmental monitoring.This method shows great potential for on-site NH_(3)detection particularly in remote area,where a simple,fast,low-cost,and easy-to-handle method is highly desirable. | Zhiwei Qiu Yitong Xue Jiyong Li Yunzhi Zhang Xinyi Liang Congying Wen Houjian Gong Jingbin Zeng | 2021 | Chinese Chemical Letters2021，32，9： | 0 |
| 15 | Single-domain antibodies for radio nuclear imaging and therapy of esophageal squamous cell carcinoma:a narrative review显示文摘Single-domain antibodies have the characteristics of small molecular weight,strong tissue penetration,and high affinity,and are widely used to construct molecular probes for disease diagnosis and treatment.This article reviews molecular imaging studies including positron emission tomography(PET),single-photon emission computed tomography/computed tomography(CT),PET/CT,and fluorescent imaging of molecular probes composed of single-domain antibodies against eight esophageal squamous cell carcinoma biological targets.These 8 targets are highly expressed on the membrane of esophageal squamous cell carcinoma cells and include epidermal growth factor receptor,human epidermal growth factor receptor 2,human epidermal growth factor receptor 3,hepatocyte growth factor receptor,vascular endothelial growth factor receptor 2,chemokine receptor 4,chemokine receptor 7,and carcinoembryonic antigen.The current problems and solutions are also discussed to provide a reference for future design of molecular imaging probes targeting esophageal squamous cell carcinoma. | Huifang Liu Xu Nie Zhenchao Tian Dan Chen Xueli Chen Qi Zeng Xinyi Xu | 2020 | Journal of Bio-X Research2020，3，4： | 0 |
| 16 | Histone variant H2A.Z facilitates DNA replication显示文摘In eukaryotes,DNA wraps around the histone octamer to form nucleosomes,which are further compacted into the chromosome structure.The chromosome contains multiple replication origins that are regulated by both DNA sequence and epigenetic features. | Lei Qiu Xinyi Zeng Junhong Han | 2020 | Science China(Life Sciences)2020，63，5： | 0 |
| 17 | Antibacterial activity of the ethyl acetate part of Abrus cantoniensis against Staphylococcus aureus显示文摘Objective:The aim of this work was to measure the antibacterial activity(against Escherichia coli and Staphylococcus aureus[S.aureus])of the ethyl acetate part of Abrus cantoniensis and assess their potential as medicines.Methods:The experiment was divided into four groups:negative control group[with Mueller-Hinton broth(MHB)],positive control group(with 75%ethanol),blank group(with MHB)and test group(with the ethyl acetate part of Abrus cantoniensis).The antibacterial activities of the extracts were evaluated by the Oxford cup assay and minimum inhibitory concentration(MIC).Time-kill curve experiments,scanning electron microscopy,the content of DNA,RNA and protein were used to study the antibacterial mechanism of the ethyl acetate extract part on the growth and viability of S.aureus.The study procedures were approved by the Animal Care and Use Committee of Xi’an Jiaotong University(approval No.XJTULAC2016-412)on January 22,2016.Results:The ethyl acetate part of Abrus cantoniensis extract exhibited the highest inhibitory activity against the growth of S.aureus with an inhibition zone diameter of 16.4mm and MIC value of 0.5mg/mL.The general activity range of the ethyl acetate part,determined using a time-killing curve,was found to be 0.5mg/mL to 40mg/mL(MIC to 80MIC).Changes in the scanning electron microscopy images and of DNA,RNA and proteins of S.aureus indicated possible mechanisms of the inhibitory activity of the ethyl acetate part.Conclusion:The ethyl acetate part of Abrus cantoniensis damaged bacterial cell structures,which results in protoplasm leakage,and eventually bacterial cell death. | Xu Nie Huifang Liu Xinyi Xu Yonghua Zhan Hui Xie Qi Zeng | 2021 | Journal of Bio-X Research2021，4，2： | 0 |
| 18 | Sir Run Run Shaw Hospital leads the establishment of the first National Engineering Research Center in the field of minimally invasive medicine in China显示文摘With the rapid development of science and technology,the minimally invasive surgery(MIS)has become a reality.MIS is associated with less pain,a shorter hospital stay and fewer complications.The higher-quality minimally invasive instruments allow the surgeons to perform the surgical interventions efficiently without sacrificing patient safety.Therefore,the academic research,technology development,achievement transformation as well as clinical application of minimally invasive devices is extremely important。 | Qingjie Zeng Xinyi Wu Yifan Wang | 2022 | Laparoscopic, Endoscopic and Robotic Surgery2022，5，4： | 0 |
| 19 | The development and application of dual-comb spectroscopy in analytical chemistry显示文摘Analytical chemistry plays an important role in the qualitive and quantitative analysis for molecules in the various circumstances,especially for the high-resolution analysis.The dual-comb spectroscopy(DCS)technology with the characteristics of high resolution,high sensitivity and instantaneous sampling exhibited a great potential in high-resolution in-situ spectral methods and has been active in the fields of spatial ranging,air composition analysis,reaction monitoring and so on.In this review,we will summarize the principle of DCS according to the different wavelength coverage and overview the applications of DCS in analytical chemistry. | Ziheng Wei Xinyi Ren Ming Yan Hulie Zeng | 2023 | Chinese Chemical Letters2023，34，1： | 0 |
| 20 | 0D/3D direct Z-scheme heterojunctions hybridizing by MoS2 quantum dots and honeycomb conjugated triazine polymers (CTPs) for enhanced photocatalytic performance显示文摘Herein,a novel direct Z-scheme photocatalyst was accomplished by hybridization of 0D MoS2 quantum dots(MSQDs)and 3D honeycomb-like conjugated triazine polymers(CTP)(namely,CTP-MSQD).The unique 0D/3D hierarchical structure significantly enhanced the exposure of active sites and light harvesting property,while the formed p-n junction enabled the direct strong interface coupling without the necessity of any mediators.The optimized CTP-MSQD3 exhibited continuously increased visible-light-driven photocatalytic activity and strong durability both in Cr(VI)reduction and H2 evolution,featured a rate of 0.069 min^(-1) and 1070μmol/(hr•g),respectively,which were 8 times than those of pure 3D-CTP(0.009 min^(−1) and 129μmol/(hr•g)).We believe that this work provides a promising photocatalyst system that combines a 0D/3D hierarchical structure and a Z-scheme charge flow for efficient and stable photocatalytic conversion. | Shuqi Li Weishun Lai Xinming Jiang Yashuang Wang Xinyi Cai Da Wang Shuang Song Min Liu Tao Zeng | 2023 | Journal of Environmental Sciences2023，，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员