|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effects of Water-table Depth and Soil Moisture on Plant Biomass, Diversity, and Distribution at a Seasonally Flooded Wetland of Poyang Lake, China显示文摘Hydrological regime has been widely recognized as one of the major forces determining vegetation distribution in seasonally flooded wetland. Poyang Lake, the largest freshwater lake in China, has been encountering dramatic changes in hydrological conditions in last decade, which greatly influenced the wetland vegetations. To explore the relationships between hydrology and vegetation distribution, water-table depth, soil moisture, species composition, diversity and biomass were measured at a seasonally flooded wetland section at Wucheng National Nature Reserve. Three plant communities, Artemisia capillaris, Phragmites australis and Carex cinerascens communities, were examined which are zonally distributed from upland to lakeshore with decreasing elevation. Canonical correspondence analysis(CCA), spearmen correlation and logistic regression were adopted to analyze the relationships between vegetation characteristics and hydrological variables of water-table depth and soil moisture. Results show that significant hydrological gradient exist along the wetland transect. Water-table demonstrates a seasonal variation and is consistently deepest in A. capillaris community(ranging from –0.5 m above ground to +10.3 m below ground), intermediate in P. australis community(–2.6 m to +7.8 m) and shallowest in C. cinerascens community(–4.5 m to +6.1 m). Soil moisture is lowest and most variable in A. capillaris community, highest and least variable in P. australis community, and intermediate and moderate variable in C. cinerascens community. The CCA ordination indicated that variables of water-table depth and soil moisture are strongly related to community distribution, which explained 81.7% of the vegetation variations. Species diversity indices are significantly positively correlated with soil moisture and negatively correlated with moisture variability, while above- and belowground biomass are positively correlated with moisture. Above- and belowground biomass present Gaussian models along the gradient of average water-table depth in growing season, while species diversity indices show bimodal patterns. The optimal average water-table depths for above- and belowground biomass are 0.8 m and 0.5 m, respectively, and are 2.2 m and 2.4 m for species richness and Shannon-Wiener indices, respectively. Outcomes of this work improved the understandings of the relationship between hydrology and vegetation. | XU Xiuli ZHANG Qi TAN Zhiqiang LI Yunliang WANG Xiaolong | 2015 | Chinese Geographical Science2015，25，6： | 24 |
| 2 | Mechanical mechanism of overlying strata breaking and development of fractured zone during close-distance coal seam group mining显示文摘This study mainly investigates the mechanical mechanism of overlying strata breaking and the development of fractured zones during close-distance coal seam group mining in the Gaojialiang coal mine.First,a mechanical model for the second'activation'of broken overlying strata is established,and the related mechanical'activation'conditions are obtained.A recursive formula for calculating the separation distance of overlying strata is deduced.Second,a height determining method for predicting the height of fractured zones during close-distance coal seam group mining is proposed based on two values,namely,the separation distance and ultimate subsidence value of overlying strata.This method is applied to calculate the fractured zone heights in nos.20107 and 20307 mining faces.The calculated results are almost equal to the field observation results.Third,a modified formula for calculating the height of a waterflowing fractured zone is proposed.A comparison of the calculated and observed results shows that the errors are small.The height determining method and modified formula not only build a theoretical foundation for water conservation mining at the Gaojialiang coal mine,but also provide a reference for estimating the height of water-flowing fractured zones in other coal mines with similar conditions. | Jianguo Ning Jun Wang Yunliang Tan Qiang Xu | 2020 | International Journal of Mining Science and Technology2020，30，2： | 19 |
| 3 | A new criterion of coal burst proneness based on the residual elastic energy index显示文摘To evaluate the coal burst proneness more precisely,a new energy criterion namely the residual elastic energy index was proposed.This study begins by performing the single-cyclic loading-unloading uniaxial compression tests with five pre-peak unloading stress levels to explore the energy storage characteristics of coal.Five types of coals from different mines were tested,and the instantaneous destruction process of the coal specimens under compression loading was recorded using a high speed camera.The results showed a linear relationship between the elastic strain energy density and input energy density,which confirms the linear energy storage law of coal.Based on this linear energy storage law,the peak elastic strain energy density of each coal specimen was obtained precisely.Subsequently,a new energy criterion of coal burst proneness was established,which was called the residual elastic energy index(defined as the difference between the peak elastic strain energy density and post peak failure energy density).Considering the destruction process and actual failure characteristics of coal specimens,the accuracy of evaluating coal burst proneness based on the residual elastic energy index was examined.The results indicated that the residual elastic energy index enables reliable and precise evaluations of the coal burst proneness. | Fengqiang Gong Yunliang Wang Zhiguo Wang Junfeng Pan Song Luo | 2021 | International Journal of Mining Science and Technology2021，31，4： | 16 |
| 4 | Picroside Ⅱ down-regulates matrix metalloproteinase-9 expression following cerebral ischemia/reperfusion injury in rats显示文摘Studies have shown that Picroside II attenuates inflammatory reactions following brain ischemia through the inhibition of the TLR-4-NF-κB signal transduction pathway,and ameliorates cerebral edema through the reduction of aquaporin-4 expression.Matrix metalloproteinase-9 (MMP-9),located downstream of the TLR-4-NF-κB signal transduction pathway,can degrade the neurovascular matrix,damage the blood-brain barrier to induce cerebral edema,and directly result in neuronal apoptosis and brain injury.Therefore,the present study further observed MMP-9 expression in the brain tissues of rats with cerebral ischemia/reperfusion injury following Picroside II treatment.Results demonstrated that Picroside II significantly reduced MMP-9 expression in ischemic brain tissues,as well as neuronal apoptosis and brain infarct volume,suggesting Picroside II exhibits neuroprotection by down-regulating MMP-9 expression and inhibiting cell apoptosis. | Xiang Li Xinying Xu Zhen Li Yunliang Guo Qin Li Xiaodan Li Zhen Zhou | 2010 | Neural Regeneration Research2010，5，18： | 13 |
| 5 | Chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and nonalcoholic steatohepatitis via angiogenesis rather than endoplasmic reticulum stress显示文摘In the present study,we aimed to investigate the role of endoplasmic reticulum stress(ERS)and its related inflammation and angiogenesis in liver fibrosis in a rat model of combined hypoxia and nonalcoholic steatohepatitis(NASH)and to confirm whether the intervention of hypoxia-inducible factor 1α(HIF1α)can improve fibrosis.Liver histological changes and biochemical indices,HIF1α,inflammatory factors,ERS-related parameters(GRP78,CHOP,caspase-3,and caspase-12),and angiogenesis indices(VEGFA,VEGFR2,and CD34)were evaluated.Compared with the control rats,the liver tissue of rats with hypoxia and NASH had obvious NASH characteristics and hepatic fibrosis was significantly aggravated,including bridging fibrosis in some rats.The mRNA expression levels of HIF1α,VEGFA,and VEGFR2 and total immunohistochemical staining scores of VEGFR2 and CD34 were significantly increased.In addition,HIF1α silencing significantly decreased HIF1α,biochemical indices(ALT,AST,and TG),inflammatory factors(TNFα,IL6,and IL1β),and angiogenesis indices(CD34 and VEGFR2),consequently,improved the hepatic fibrosis score in the rat model of combined hypoxia and NASH.Taken together,chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and NASH via angiogenesis rather than ERS and HIF1α intervention can improve liver fibrosis,angiogenesis,inflammatory factors,and biochemical indices.Therefore,HIF1α is a key regulatory factor of liver fibrosis in rats with combined hypoxia and NASH. | Wei Wu Weiping Li Jiaojiao Wei Chunsheng Wang Yunliang Yao Weihua Zhu Weimei He Weimei Zhou Jiang Liu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，2： | 8 |
| 6 | Anti-oxidant effect of picroside II in a rat model of cerebral ischemia/reperfusion injury显示文摘Picroside II,the major active component of picroside,has been shown to induce PC12 cell axonal growth and relieve free radical damage.In vivo experiments have demonstrated that picroside II can improve neurological function in rats with cerebral ischemia/reperfusion injuries.In the present in vivo study,enzyme-linked immunosorbent assay and immunohistochemistry revealed that picroside II increased superoxide dismutase content and reduced inducible nitric oxide synthase content in the ischemic hemisphere.The effects of picroside II were similar to those of salvianic acid A sodium,an active control drug.These results indicate that picroside II exerts a neuroprotective effect,possibly by downregulating inducible nitric oxide synthase expression,increasing superoxide dismutase activity,and inhibiting neuronal apoptosis. | Li Sun Xiaodan Li Ling Wang Lihua Qin Yunliang Guo Zhen Zhou | 2011 | Neural Regeneration Research2011，6，15： | 8 |
| 7 | Recent Progress in Automotive Gasoline Direct Injection Engine Technology显示文摘Gasoline direct injection(GDI)engines are currently the dominant powertrains for passenger cars.With the implementation of increasingly stringent fuel consumption and emission regulationsworldwide,GDI engines are facing challenges owing to high particulate matter emissions and a tendency to knock,leading to a change in the research and design(R&D)issues compared with those in the twentieth century.This paper reviews the progress in research regarding GDI engine technologies over the past 20 years,focusing on combustion system configurations,and also highlights common issues in GDI R&D,including pre-ignition and deto-knock,soot formation and PM emissions,injector deposits and gasoline compression ignition(GCI).First,an overview of recent developments in the field as driven by regulations is provided,following which progress in injection and combustion systems is examined.Third,the review addresses the occurrence and mechanism of deto-knock and considers means of suppressing this phenomenon.The fourth section discusses soot formation mechanisms and particulate matter emission characteristics of GDI engines and describes the application of gasoline particulate filter(GPF)after-treatment.The subsequent section summarizes studies regarding injector deposit formation,as well as pioneering research into GCI combustion modes.Finally,a summary and future prospects for GDI engine technologies are provided. | Shijin Shuai Xiao Ma Yanfei Li Yunliang Qi Hongming Xu | 2018 | Automotive Innovation2018，1，2： | 8 |
| 8 | Change of Annual Extreme Water Levels and Correlation with River Discharges in the Middle-lower Yangtze River: Characteristics and Possible Affecting Factors显示文摘As one of the fastest developing regions in China, the middle-lower Yangtze River(MLYR) is vulnerable to floods and droughts. With obtained time series of annual highest water level(HWL), annual lowest water level(LWL) and the corresponding river discharges from three gauging stations in MLYR that covering the period 1987–2011, the current study evaluated the change characteristics of annual extreme water levels and the correlation with river discharges by using the methods of trend test, Mann-Whitney-Pettitt(MWP) test and double mass analysis. Major result indicated a decreasing/increasing trend for annual HWL/LWL of all stations in MLYR during the study period. A change point in 1999 was identified for annual HWL at the Hankou and Datong stations. The year 2006 was found to be the critical year that the relationship between annual extreme water levels and river discharges changed in the MLYR. With contrast to annual LWL in MLYR, further investigation revealed that the change characteristics of annual HWL were highly consistent with regional precipitation in the Yangtze River Basin, while the linkage with Three Gorges Dam(TGD) operation is not strong. Our observation also pointed out that the effect of serious down cutting of the riverbed and the enlargement of the cross-section area during the initial period of TGD operation caused the downward trend of the relationship between annual LWL and river discharge. Whereas, the relatively raised river water level before the flood season due to TGD regulation since 2006 explained for the changing upward trend of the relationship between annual HWL and river discharge. | YE Xuchun XU Chong-Yu LI Yunliang LI Xianghu ZHANG Qi | 2017 | Chinese Geographical Science2017，27，2： | 7 |
| 9 | Similar simulation study on the deformation and failure of surrounding rock of a large section chamber group under dynamic loading显示文摘Large and super-large section chamber groups in coal mines are frequently affected by dynamic loads resulting from production activities such as roadway driving and blasting.The stability of the surrounding rock is poor,and it is difficult to control.In this paper,a similar simulation test was used to study the deformation and evolution laws of the surrounding rock of a triangle-shaped chamber group under different dynamic loads.The results showed that under dynamic loading,the vertical stress of the surrounding rock of the chamber group increased in an oscillatory form.The maximum stress concentration coefficient reached 4.09.The damage degree of the roof was greater than that of the two sides.The deformation of the roof was approximately 1.2 times that of the two sides.For the chamber closer to the power source,the stress oscillation amplitude of the surrounding rock was larger,and the failure was more serious.The force of the anchorage structure showed a phased increasing characteristic;additionally,the force of the anchorage structure on the adjacent side of the chambers was greater than that on the other side.This study reveals the deformation and failure evolution laws of the surrounding rock of large section chamber groups under dynamic loading. | Xuesheng Liu Shilin Song Yunliang Tan Deyuan Fan Jianguo Ning Xuebin Li Yanchun Yin | 2021 | International Journal of Mining Science and Technology2021，31，3： | 6 |
| 10 | Quantized Hybrid Precoding Design for Millimeter-Wave Large-Scale MIMO Systems显示文摘Millimeter wave(mmWave) and large-scale multiple input multiple output(MIMO) are two emerging technologies in fifth-generation wireless communication systems. The power consumption and hardware cost of radio frequency(RF) chains increase exponentially with the bit resolution of analog-to-digital converters(ADCs) and digital-to-analog converters(DACs). One promising solution is to employ few RF chains with low-bit ADCs and DACs. In this paper, we consider mmWave large-scale MIMO systems with low bits DACs and ADCs. Leveraging on the Bussgang theorem and the additive quantization noise model(AQNM), a closed-form expression of the achievable rate is derived to show the effect of the ADCs? and DACs? resolution. Moreover, an orthogonal matching pursuit(OMP) based hybrid precoding algorithm is proposed to increase the achievable rate. Our results show that the impact of DACs is more pronounced than the impact of ADCs. Furthermore, 5-bit ADCs and DACs are sufficient at the transceiver to operate without a significant performance loss. | Zelin Lu Yunliang Zhang Jiayi Zhang | 2019 | China Communications2019，16，4： | 4 |
| 11 | Synthesis of montmorillonite-chitosan hollow and hierarchical mesoporous spheres with single-template layer-by-layer assembly显示文摘In order to develop a facile and precisely controlled approach to synthesize hierarchical mesoporous materials with tailored property, in this work, a novel study was carried out to fabricate montmorillonitechitosan hollow and hierarchical mesoporous spheres(MMTNS@CS-HMPHS) based on single-template layer-by-layer(Lb L) assembly. Scanning electron microscopy(SEM), transmission electron microscopy(TEM), specific surface area analysis and X-ray photoelectron spectroscopy(XPS) analyses were carried out to characterize the morphology and surface properties of MMTNS@CS-HMPHS. Benefitting from the unique lamellar structure of MMTNS, mesoporous channels are formed on the shell of MMTNS@CS hollow spheres, resulting in high surface area. Moreover, the surface functionalization and pore size of MMTNS@CS-HMPHS can be easily tuned, due to the tailored property through Lb L assembly method.Besides the unique microstructure, MMTNS@CS-HMPHS also possesses the active sites generated from both MMT and chitosan, which greatly promotes its performance in fields of adsorption, drug delivery and catalyst supports, etc. | Peng Chen Yunliang Zhao Tianxing Chen Tingting Zhang Shaoxian Song | 2019 | Journal of Materials Science & Technology2019，35，10： | 4 |
| 12 | Microstructure and Mechanical Properties of Mg–3Al–Zn Magnesium Alloy Sheet by Hot Shear Spinning显示文摘Hot shear spinning experiments with Mg–3.0 Al–1.0 Zn–0.5 Mn(AZ31 B, wt%) magnesium alloy sheets were conducted at various temperatures, spindle speeds and feed ratios to investigate the effects of these processing parameters on the microstructure, crystallographic texture and mechanical properties. The AZ31 B sheet displayed good shear formability at temperatures from 473 to 673 K, spindle speeds from 300 to 600 rev/min and feed ratios from 0.1 to 0.5 mm/rev. During the dynamic recrystallization process, the grain size and texture were affected by the deformation temperature of the hot shear spinning process. Each of the spun sheets presented a strong basal texture, and the c-axis of most of the grains was parallel to the normal direction. The optimal hot shear spinning parameters were determined to be a temperature of 473 K, a spindle speed of 300 rev/min and a feed ratio of 0.1 mm/rev. The yield strength, ultimate tensile strength and elongation in the rolled direction reached 221 MPa, 288 MPa and 14.1%, and those in the transverse direction reached 205 MPa, 280 MPa and 12.4%, respectively. The improved strength and decreased mechanical anisotropy resulted from the fine grain size and strong basal texture. | Fenghua Wang Peng Su Linxin Qin Shuai Dong Yunliang Li Jie Dong | 2020 | Acta Metallurgica Sinica(English Letters)2020，33，9： | 4 |
| 13 | Deformation tests and failure process analysis of an anchorage structure显示文摘In order to study the failure process of an anchorage structure and the evolution law of the body’s deformation field,anchor push-out tests were carried out based on digital speckle correlation methods(DSCM).The stress distribution of the anchorage interface was investigated using the particle flow numerical simulation method.The results indicate that there are three stages in the deformation and failure process of an anchorage structure:elastic bonding stage,a de-bonding stage and a failure stage.The stress distribution in the interface controls the stability of the structure.In the elastic bonding stage,the shear stress peak point of the interface is close to the loading end,and the displacement field gradually develops into a‘‘V’’shape.In the de-bonding stage,there is a shear stress plateau in the center of the anchorage section,and shear strain localization begins to form in the deformation field.In the failure stage,the bonding of the interface fails rapidly and the shear stress peak point moves to the anchorage free end.The anchorage structure moves integrally along the macro-crack.The de-bonding stage is a research focus in the deformation and failure process of an anchorage structure,and plays an important guiding role in roadway support design and prediction of the stability of the surrounding rock. | Zhao Tongbin Yin Yanchun Tan Yunliang Song Yimin | 2015 | International Journal of Mining Science and Technology2015，25，2： | 4 |
| 14 | Ore-controlling effect of structure and distribution of deep rock mass on Pb-Zn deposit in Qingchengzi ore concentration area,Liaoning显示文摘Qingchengzi ore concentration area in Liao-Ji rift is an important lead-zinc ore area of China,and the deep prospecting in this area has great prospects.Based on the spatial occurrence of ore bodies the Pb-Zn deposit can be divided into three types:layered,vein-shaped and pinnate.The deep geological conditions in this area are deduced by analyzing the tectonic evolution process and rock mass gravity inversion.The tectonic evolution of Liao-Ji rift can be divided into three stages:Paleoproterozoic extension period,Mesoproterozoic compression period and Mesozoic reactivation period.The magmatic activities in the Indosinian epoch led to the distribution pattern of the present deposits.According to the gravity inversion,Shuangdinggou-and Xinling rock masses on the north and south sides of the mining area are connected in the deep.The connected rock body might be distributed in the entire mining area.Xinling rock mass may be a branch extending from Shuangdinggou rock mass along the northeast trending fault,the connected rocks provide magmatic hydrothermal fluid for the final,folds and faults result in different types of ore body shapes. | NIU Mingxuan YU Yunliang TIAN Liya NIU Wenqing XU Haibo | 2020 | Global Geology2020，23，2： | 4 |
| 15 | A Layout Optimization Method of Composite Wing Structures Based on Carrying Efficiency Criterion显示文摘二水平的布局优化策略为大规模合成翅膀结构在这份报纸被建议。设计要求根据结构的变丑在系统水平被调整,当布局在从系统水平满足限制的分系统水平被优化时。各种各样的失败的来临的度在翅膀面板的批评负担被采用估计结构正在带效率。由作为一个目的优化效率,这个问题的连续性能被保证。使硬的翅膀面板被相等的 orthotropic 盘子建模,并且全球弄弯负担被精力方法预言。stringer 皮肤本地人扣环抵抗上的支持弹性的非线性的效果被神经网络(NN ) 调查并且接近代理人模型。这些失败预言基于分析答案,它能有效地节省计算资源。最后,大规模翅膀结构的不可分的优化作为一个例子被完成。结果完成设计要求并且显示出这个方法的可行性。 | ZHAO Qun DING Yunliang JIN Haibo | 2011 | Chinese Journal of Aeronautics2011，24，4： | 4 |
| 16 | Comparison of Charlson's weighted index of comorbidities with the chronic health score for the prediction of mortality in septic patients显示文摘 | Cui Yunliang Wang Tao Bao Jun Tian Zhaotao Lin Zhaofen Chen Dechang | 2014 | Chinese Medical Journal2014，，14： | 4 |
| 17 | Survivin-specific small interfering RNAs enhance sensitivity of glioma U-87MG cells to paclitaxel by promoting apoptosis显示文摘A survivin siRNA expression vector was transfected into glioma U-87MG cells and these cells were then treated with paclitaxel.The results showed that survivin-specific siRNA combined with paclitaxel treatment synergistically inhibited glioma U-87MG cell proliferation and promoted apoptosis.This treatment also inhibited the expression of the cell cycle regulatory proteins,survivin,cyclinD1,c-Myc and CDK4 and enhanced the sensitivity of U-87MG cells to paclitaxel. | Yunliang Xie Yanbo Liu Weigao Shen Bo Zhang Qun Liu | 2012 | Neural Regeneration Research2012，7，13： | 3 |
| 18 | Pretreatment of biomass by torrefaction显示文摘Agricultural biomass has some drawbacks such as high moisture content,low energy density and wide distribution and as a result,the cost of transport and storage are high.Moreover,raw biomass has poor grindability so its use in a pulverized boiler or entrained flow gasifier is difficult.Torrefaction is a mild pyrolysis process carried out at temperatures ranging from 200°C to 300°C to deal with these problems.The cotton stalk and wheat straw were torrefied in a fix-bed reactor at moderate temperatures (200°C,230°C,250°C,270°C and 300°C) under N 2 for 30min.The biomass chars after torrefaction had higher energy density and improved grindability characteristics compared with raw biomass and they also showed hydrophobic characteristics.The volatiles consist of a condensable fraction and a non-condensable fraction.The former mainly contained water and tar (organic products but mainly acetic acid).The non-condensable products are typically comprised of CO 2,CO and a small amount of CH 4 and even trace H 2.The volatiles increased with an increase in the torrefaction temperature but the solid yield and the energy yield decreased.However,the grindability and energy density of the biomass char showed great improvement.A kinetic study on the generation of the main non-condensable gases was undertaken and we conclude that the gases are formed by parallel independent first-order reactions.Characteristic kinetic parameters for the generation of each gas were determined. | WANG GuiJun LUO YongHao DENG Jian KUANG JiangHong ZHANG YunLiang | 2011 | Chinese Science Bulletin2011，56，14： | 3 |
| 19 | Morphology of polar ionospheric O^+ ion upflow: FAST observations during quiet time显示文摘With energetic ion measurements on FAST satellite, the morphologic features of quiet-time ionospheric O+ ion upflowing at altitudes of 2000 to 4000 km are drawn out for the first time. The pre-noon cusp/cleft is the predominant region of upflowing occurrence for O+ with lower energy. Meanwhile the pre-dawn sector near the equatorward edge of the plasma convection dominates the occurrence for the higher energy O+ ions. No matter whether the energy is lower or higher, the upflows occur often over a wide MLT range of lower latitudes outside the auroral oval. The upflowing within the pre-midnight (21:00― 22:00 MLT) auroral oval carrys larger energy fluxes, with extremely large fluxes for higher energy O+ appearing near the polar cap boundary. For altitudes of 2000―4200 km under observation, the ion conics occur much more frequently than ion beams. Ion beams are rarely found below 3000 km, while the conics occur uniformly over the observed altitudes. | DANG Ge MA ShuYing ZHOU YunLiang | 2007 | Chinese Science Bulletin2007，52，24： | 3 |
| 20 | Interventional effect of phycocyanin on mitochondrial membrane potential and activity of PC12 cells after hypoxia/reoxygenation显示文摘BACKGROUND: Phycocyanin can relieve decrease of mitochondrial membrane potential through reducing production of active oxygen so as to protect neurons after hypoxia/reoxygenation. OBJECTIVE: To observe the effect of phycocyanin on activity of PC12 cells and mitochondrial membrane potential after hypoxia/reoxygenation. DESIGN: Randomized controlled study. SETTING: Cerebrovascular Disease Institute of Affiliated Hospital, Medical College of Qingdao University. MATERIALS: The experiment was carried out at the Key Laboratory of Prevention and Cure for cerebropathia in Shandong Province from October to December 2005. PC12 cells, rat chromaffin tumor cells, were provided by Storage Center of Wuhan University; phycocyanin was provided by Ocean Institute of Academia Sinica; Thiazoyl blue tetrazolium bromide (MTT) and rhodamine 123 were purchased from Sigma Company, USA; RPMI-1640 medium, fetal bovine serum and equine serum were purchased from Gibco Company, USA. METHODS: ① Culture of PC12 cells: PC12 cells were put into RPMI-1640 medium which contained 100 g/L heat inactivation equine serum and 0.05 volume fraction of fetal bovine serum and incubated in CO2 incubator at 37 ℃. Number of cells was regulated to 4 × 105 L-1, and cells were inoculated at 96-well culture plate. The final volume was 100 μL. ② Model establishing and grouping: Cultured PC12 cells were randomly divided into three groups: phycocyanin group, model control group and non-hypoxia group. At 24 hours before hypoxia, culture solution in phycocyanin group was added with phycocyanin so as to make sure the final concentration of 3 g/L , but cells in model control group did not add with phycocyanin. Cells in non-hypoxia group were also randomly divided into adding phycocyanin group (the final concentration of 3 g/L) and non-adding phycocyanin group. Cells in model control group and phycocyanin group were cultured with hypoxia for 1 hour and reoxygenation for 1, 2 and 3 hours; meanwhile, cells in non-hypoxia group were cultured with oxygen and were measured at 1 hour after hypoxia/reoxygenation. ③ Detecting items: At 1, 2 and 3 hours after reoxygenation, absorbance (A value) of PC12 cells was measured with MTT technique so as to observe activity and quantity of cells. Fluorescence intensity of PC12 cells marked by rhodamine 123 was measured with confocal microscope in order to observe changes of mitochondrial membrane potential. MAIN OUTCOME MEASURES: Comparisons between quantity and activity of PC12 cells and mitochondrial membrane potential at 1, 2 and 3 hours after reoxygenation. RESULTS: ① Effect of phycocyanin on quantity and activity of PC12 cells: A value was 0.924±0.027 in adding phycocyanin group and 0.924±0.033 in non-adding phycocyanin group. A value was lower in model control group and phycocyanin group than that in non-hypoxia group at 1, 2 and 3 hours after reoxygenation (0.817±0.053, 0.838±0.037, 0.875±0.029; 0.842±0.029, 0.872±0.025, 0.906±0.023, P < 0.05). A value was higher in phycocyanin group than that in model control group at 1, 2 and 3 after culture (P < 0.05). With culture time being longer, A value was increased gradually in phycocyanin group and model control group after reoxygenation (P < 0.05). ② Effect of phycocyanin on mitochondrial membrane potential of PC12 cells: Fluorescence intensity was 2.967±0.253 in adding phycocyanin group and 2.962±0.294 in non-adding phycocyanin group. Fluorescence intensity was lower in model control group and phycocyanin group than that in non-hypoxia group at 1, 2 and 3 hours after hypoxia/reoxygenation (1.899±0.397, 2.119±0.414, 2.287±0.402; 2.191±0.377, 2.264±0.359, 2.436±0.471, P < 0.05); but it was higher in phycocyanin group than that in model control group at 1, 2 and 3 after reoxygenation (P < 0.05). With culture time being longer, fluorescence intensity was increased gradually in phycocyanin group and model control group after reoxygenation (P < 0.05). CONCLUSION: Phycocyanin and reoxygenation can protect PC12 cells after hypoxia injury through increasing mitochondrial membrane potential and cellular activity, and the effect is improved gradually with prolonging time of reoxygenation. | Nan Jiang Yunliang Guo Hongbing Chen | 2006 | Neural Regeneration Research2006，1，2： | 3 |
您所在的机构暂未开通此部分的功能，请联系管理员