|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Engineering Introns to Express RNA Guides for Cas9- and Cpfl-Mediated Multiplex Genome Editing显示文摘定期聚类 interspaced 短 palindromic 重复(CRISPR ) 系统为指向的 DNA 作为革命站台出现了。这个系统使用一个地点特定的 RNA 指南指导一个 CRISPR 受动器(例如, Cas9 和 Cpf1 ) 到一个 DNA 目标。这里,我们详细描述一般策略同时从 Cas9 和 Cpf1 的 introns 表示多重指南 RNA (gRNA ) 和 CRISPR RNA (crRNA ) 。这个方法利用处理系统或 Cpf1 的 crRNA 处理活动劈开包含 tRNA-gRNA polycistron 或 crRNA-crRNA 数组的拼接的 intron 的内长的 tRNA。我们证明 tRNA-gRNA intron 能作为一基因与 Cas9 熔化。如此的混合基因能用一个聚合酶 II 倡导者被表示,并且在同时指向多重地点展出了高效率和坚韧性。我们 ? 也在调停 Cpf1 的染色体用 intronic tRNA-crRNA 和 crRNA-crRNA 数组编辑实现了这策略。有趣地,包含 Cpf1 和 intronic crRNA 的混合基因与常规 Cpf1 向量相比穿展出显著地增加的效率。一起拿,这研究介绍一个方法从一混合基因表示 CRISPR 试剂增加编辑染色体的效率和能力。由于它的简洁和通用性,这个方法能广泛地被用来在优核质开发复杂 CRISPR 工具。 | Dan Ding Kaiyuan Chen Yuedan Chen Hong Li Kabin Xie | 2018 | Molecular Plant2018，11，4： | 14 |
| 2 | Comparisons of plant calcium fraction between two different vegetation zones in semi-arid region显示文摘To explore the characteristics of plant calcium(Ca) fraction, we analyzed 91 plant species in the Ningxia Habahu National Nature Reserve in Yanchi County of Ningxia and 84 plant species in Zhenglan Banner of Inner Mongolia. Results show that, for the two regions, there is no significant difference between Ca fraction for the same growth type, and between water soluble Ca content or between hydrochloric acid soluble Ca content for plants of the same ecosystem and between hydrochloric acid soluble Ca content for plants of the same family. In similar vegetation zones, there is a significant difference among hydrochloric acid soluble Ca content for different growth types in Yanchi County and Zhenglan Banner,which was the highest for annual herbs and the lowest for perennial herbs. There is a significant difference between acetic acid soluble Ca content and between hydrochloric acid soluble Ca content for sandy land and grassland ecosystems in Yanchi County. There is a significant difference among the same Ca fraction of different families in the same region. Thus, the characteristics of plant Ca fraction are results of long-term adaptation to the environment. | MengQi Li XingDong He XiangXiang Yang YueDan Zhao YuBao Gao | 2018 | Research in Cold and Arid Regions2018，10，4： | 2 |
| 3 | Permutation entropy applied to movement behaviors of drosophila melanogaster显示文摘 | Liu Yuedan | 2011 | Modern Physics Letters B2011，，25： | 1 |
| 4 | Attack robustness of cascad- ing load model in interdependent networks 显示文摘 | Wang Jianwei Wu Yuedan Li Yun | 2014 | International Jour- nal of Modern Physics C2014，26，15： | 1 |
| 5 | Evaluation of stream ecosystem health and species association based on multi-taxa (benthic macroinvertebrates, algae, and microorganisms) patterning with different levels of pollution显示文摘 | Tae-Soo Chon Xiaodong Qu Woon-Seok Cho Hyun-Ju Hwang Hongqu Tang Yuedan Liu Jung-Hye Choi Myounghwa Jung Bok Sil Chung Hak Young Lee Young Ryun Chung Sung-Cheol Koh | 2013 | Ecological Informatics2013，，： | 1 |
| 6 | A Systems Genetics Approach Identifies Genes and Pathways for Type 2 Diabetes in Human Islets显示文摘 | Jalal Taneera Stefan Lang Amitabh Sharma Joao Fadista Yuedan Zhou Emma Ahlqvist Anna Jonsson Valeriya Lyssenko Petter Vikman Ola Hansson Hemang Parikh Olle Korsgren Arvind Soni Ulrika Krus Enming Zhang Xing-Jun Jing Jonathan L.S. Esguerra Claes B. Wollhei | 2012 | Cell Metabolism2012，，1： | 1 |
| 7 | TPX2 in malignantly transformed human bronchial epithelial cells by anti-benzo[ a ]pyrene-7,8-diol-9,10-epoxide显示文摘 | Lijuan Zhang He Huang Luyao Deng Ming Chu Lan Xu Juanling Fu Yunlan Zhu Xiuchun Zhang Shulin Liu Zongcan Zhou Yuedan Wang | 2008 | Toxicology2008，，1： | 1 |
| 8 | Treatment of secondary central nervous system involvement in systemic aggressive B cell lymphoma using R-MIADD chemotherapy:a single-center study显示文摘Background:Secondary central nervous system lymphoma(SCNSL)is defined as lymphoma involvement within the central nervous system(CNS)that originated elsewhere,or a CNS relapse of systemic lymphoma.Prognosis of SCNSL is poor and the most appropriate treatment is still undetermined.Methods:We conducted a retrospective study to assess the feasibility of an R-MIADD(rituximab,high-dose methotrexate,ifosfamide,cytarabine,liposomal formulation of doxorubicin,and dexamethasone)regimen for SCNSL patients.Results:Nineteen patients with newly diagnosed CNS lesions were selected,with a median age of 58(range 20 to 72)years.Out of 19 patients,11(57.9%)achieved complete remission(CR)and 2(10.5%)achieved partial remission(PR);the overall response rate was 68.4%.The median progression-free survival after CNS involvement was 28.0 months(95%confidence interval 11.0–44.9),and the median overall survival after CNS involvement was 34.5 months.Treatment-related death occurred in one patient(5.3%).Conclusions:These single-centered data underscore the feasibility of an R-MIADD regimen as the induction therapy of SCNSL,further investigation is warranted. | Yuchen Wu Xuefei Sun Xueyan Bai Jun Qian Hong Zhu Qu Cui Ruixian Xing Yuedan Chen Qing Liu Wenyuan Lai Junhong Li Yaming Wang Shengjun Sun Nan Ji Yuanbo Liu | 2021 | Chinese Neurosurgical Journal2021，7，2： | 0 |
| 9 | Polymodal Functionality of C. elegans OLL Neurons in Mechanosensation and Thermosensation显示文摘Sensory modalities are important for survival but the molecular mechanisms remain challenging due to the polymodal functionality of sensory neurons. Here, we report the C. elegans outer labial lateral(OLL) sensilla sensory neurons respond to touch and cold. Mechanosensation of OLL neurons resulted in cell-autonomous mechanically-evoked Ca~(2+) transients and rapidly-adapting mechanoreceptor currents with a very short latency.Mechanotransduction of OLL neurons might be carried by a novel Na~+ conductance channel, which is insensitive to amiloride. The bona fide mechano-gated Na~+ -selective degenerin/epithelial Na~+ channels, TRP-4, TMC, and Piezo proteins are not involved in this mechanosensation.Interestingly, OLL neurons also mediated cold but not warm responses in a cell-autonomous manner. We further showed that the cold response of OLL neurons is not mediated by the cold receptor TRPA-1 or the temperaturesensitive glutamate receptor GLR-3. Thus, we propose the polymodal functionality of OLL neurons in mechanosensation and cold sensation. | Yuedan Fan Wenjuan Zou Jia Liu Umar Al-Sheikh Hankui Cheng Duo Duan Du Chen Siyan Liu Luyi Chen Jilei Xu Firdosh Ruhomutally Lijun Kang | 2021 | Neuroscience Bulletin2021，37，5： | 0 |
| 10 | β2 Adrenergic receptor on T lymphocytes and its clinical implications显示文摘Sustained complex cross-talk between the immune system and the nervous system plays a vital role in retaining homeostasis in a healthy individual.One of the central regulatory mechanisms involved is the existence and functions of β2-adrenergic receptors(β2AR) on T lymphocytes.This article reviews research progress made recently,including the expression of adrenergic receptors on T lymphocytes,the structure and intracellular pathways of β2AR,the activation of β2AR by either endogenous or exogenous agonists,and the effect of β2AR stimulation on T cells which alters T cell proliferation,differentiation,cytokine production and T-helper-mediated antibody production.Furthermore,we discuss the roles of β2AR played in the pathogenesis and treatment of autoimmune diseases. | Xuelai Fan Yuedan Wang | 2009 | Progress in Natural Science:Materials International2009，19，1： | 0 |
| 11 | Characteristics of TCRαβ + CD4 - CD8- thymocytes in fetal thymic organ culture显示文摘TCRαβ+CD4-CD8- (TCR+ DN) thymocytes at different developmental periods, i.e. after either 9 or 18 days of culture in the fetal thymic organ culture (FTOC) system, were characterized in the properties of phenotype, proliferation, differentiation and apoptosis. The results showed that anti-CD3 mAb significantly promoted proliferation of TCRαβ+ DN cells generated after 18 days of culture in FTOC, whereas the cells generated after 9 days of culture responded to anti-CD3 mAb by proliferation weakly. IL-7 efficiently induced TCRαβ+ DN cells at day 9 of FTOC to differentiate into TCRαβ+CD4+/CD8+ SP cells without detectable transitional stage of TCRαβ+CD4+CD8+ (DP) cells. In contrast, fewer TCRαβ+ DN cells generated after 18 days of FTOC were induced to differentiate into SP cells. The thymic stromal cell line MTEC5 cells synergized with IL-7 to promote the differentiation of TCRαβ+ DN cells. In addition, TCRαβ+ DN cells were shown to be less susceptible to apoptosis compared with the other major thymocyte subsets. Taken together, these data have provided insight into the characteristics of TCRαβ+ DN thymocytes. | XIAO Shiyun , LI Yan , QIAN Xiaoping, WANG Yuedan and CHEN Weifeng(Department of Immunology, Peking University Health Science Center, Beijing 100083, China) | 2003 | Progress in Natural Science:Materials International2003，13，6： | 0 |
| 12 | Features on N/P ratio of plants with different functional groups between two types of steppe in semi-arid area显示文摘The differences in nitrogen/phosphorus(N/P)ratios of different functional groups in ecology are more helpful in explaining species competition and community dynamics.Based on the functional groups of plant growth type,carbon metabolism pathway,root type and phylogenetic type,we analyzed characteristics of leaf N/P ratios of 77 species in Sanggendalai(typical grassland zone)of Zhenglan Banner,Inner Mongolia,China and 91 species in the Habahu National Nature Reserve(desertified grassland zone)in Yanchi County of Ningxia,China.The results show that the N/P ratio(16.91)of C3 plants in the desertified steppe was significantly larger than that(12.72)in the typical steppe,but there was no significant difference between the N/P ratios of C4 plants in the two zones.There was no significant difference in N/P ratios between C3 plants and C4 plants in the same zone.Similarly,the N/P ratio(16.60)of dicotyledons in desertified steppe were significantly higher than that(12.98)in typical steppe,while differences in N/P ratios between monocotyledonous plants of the two zones was not significant,and there existed no significant difference in N/P ratios between dicotyledonous and monocotyledonous plants in the same zone.The N/P ratio had significant difference between gramineous and non-gramineous plants in the typical steppe but not in the desertified steppe,but there existed no significant difference in N/P ratios among different root types of perennial herbaceous plants in the same type of steppe or between two types of steppe.Thus,different features on the N/P ratios of C3 plants and dicotyledonous plants between typical steppe and desertified steppe may lead to different growth status of plants,and the N/P ratio stoichiometric of the same plant functional group may be a foundation of the changes of a plant community. | YueDan Zhao XingDong He Lei Chen XinFeng Ding MengQi Li PingYi Xu YuBao Gao | 2019 | Research in Cold and Arid Regions2019，11，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员