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| 1 | Incidence of Extended-Spectrum β-Lactamases and Characterization of Integrons in Extended-Spectrum β-Lactamase-producing Klebsiella pneumoniae Isolated in Shantou, China显示文摘这研究涉及生产 Klebsiella pneumoniae 的抗菌素抵抗 ofextended 光谱 beta-lactamase (ESBL ) 的水平,在 Shantou,中国,和它的机制孤立。74 非重复临床 K 孤立。生产在 2 年的一个时期上孤立的 ESBLswere 的 pneumoniae。抗菌素危险性,由 Epsilometer 测试执行了,证明大多数 isolates 是多抵抗的。聚合酶链反应证明在 beta-lactamases 的几种类型之中, SHV 是最流行的, TEM 是第二个大多数流行,并且 CTX-M 是最不流行的。69 孤立为 integrase 基因 IntI1 是积极的,但是没有 IntI2 或 IntI3 基因被发现。班 1 integrons 的可变区域被定序放大并且进一步识别了。13 个不同基因盒子和 11 不同盒子联合被检测。Dfr 和 aadA 盒子是占优势的,盒子 combinationsdfrA12, orfF 和 aadA2 最经常被发现。编码 ESBL 的基因盒子都不是 found.Integrons 是流行的并且起了一个重要作用在多在生产 ESBL K.pneumoniae 的药抵抗。 | Fen YAO Yuanshu QIAN Shuzhen CHEN Peifen WANG Yuanchun HUANG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，7： | 9 |
| 2 | Potential functions of esophageal cancer-related gene-4 in the cardiovascular system显示文摘Esophageal cancer-related gene-4(Ecrg4)is cloned from the normal epithelium of the esophagus.It is constitutively expressed in quiescent epithelial cells and downregulated during tumorigenesis,and Ecrg4 expression levels are inversely correlated with the malignant phenotype of tumor cells,validating that Ecrg4 is a real tumor suppressor gene.Unlike other tumor suppressor genes that usually encode membrane or intracellular proteins,Ecrg4 encodes a 148-amino acid pre-pro-peptide that is tethered on the cell surface in epithelial cells,specialized epithelial cells,and human leukocytes,where it can be processed tissue dependently into several small peptides upon cell activation.Ecrg4 is expressed in a wide variety of other cells/tissues,including cardiomyocytes and conduction system of the heart,,the glomus cells of the carotid body,adrenal glands,choroid plexus,and leukocytes among others,where it exerts distinct functions,such as promoting/suppressing inflammation,inducing neuron senescence,stimulating the hypothalamus--pituitary--adrenal axis,maintaining the stemness of stem cells,participating in the rhythm and rate control of the heart,and possibly gauging the responsiveness of the cardiovascular system(CVS)to hypoxia,in addition to tumor suppression.Here,we briefly review the latest discoveries on Ecrg4 and its underlying molecular mechanisms as a tumor suppressor and focus on the emerging roles of Ecrg4 in the CVS. | Rui Zhou Yuanshu Liu Wenjun Huang Xitong Dan | 2019 | Frontiers of Medicine2019，13，6： | 2 |
| 3 | Comparative proteomic analysis of the effects of high-concentrate diet on the hepatic metabolism and inflammatory response in lactating dairy goats显示文摘Background: To understand the impact of feeding a high-concentrate diet to mid-lactating goats for a long time on liver metabolism and inflammatory response, two dimensional polyacrylamide gel electrophoresis(2-DE) and real-time PCR method were employed to detect proteins differentially expressed in liver and their m RNAs expression in goats fed high concentrate diet(HC) or low concentrate diet(LC). Twelve lactating dairy goats were randomly assigned to either a HC diet group(65 % concentrate of dry matter; n = 6) or a LC diet group(35 % concentrate of dry matter; n = 6) for 10 wk.Results: Twenty differentially expressed spots(≥2.0-fold changes) in the hepatic tissues were excised and successfully identified using MALDI TOF/TOF. Of these, 8 proteins were up-regulated, while the rest 12 proteins were down-regulated in HC goats compared to LC. Differential expressed proteins including alpha enolase 1(ENO1), glutamate dehydrogenase 1(GLUD1), glutathione S-transferase A1(GSTA1), ATP synthase subunit 5β(ATP5β), superoxide dismutase [Cu-Zn](SOD1), cytochrom c oxidase subunit Via(COX6A1) and heat shock protein 60(HSP60) were further verified by real-time PCR and/or western blot at m RNA or protein expression level. Consistent with the 2-DE results, a significant decrease of β-actin protein expression and SOD enzyme activity was observed in liver of HC goats(P < 0.05), while ENO1 protein expression was significantly up-regulated in HC compared to LC goats(P < 0.05). However, western blot analysis did not show a significant difference of hepatic HSP60 protein between HC and LC group, which did not match the decrease of HSP60 content detected by 2-DE analysis. Real-time PCR showed that glutathione S-transferase P1(GSTP1) and SOD1 m RNA expression was significantly decreased in liver of HC goats, while cytochrom c oxidase(COX3) and ATPase 8(ATP8) m RNAs expression were markedly increased compared to LC(P < 0.05). Gene Ontology(GO) analysis revealed that HC diet resulted in altered expression of proteins related to catalytic and mitochondrial metabolism in the liver, and may increase the stress response with up-regulating the expression of differentiation 14(CD14) cluster and serum amyloid A(SAA) as well as C-reactive protein(CRP) in the liver.Conclusions: These results suggest that feeding high concentrate diet to lactating goats for 10 wk leads to the activation of the inflammatory response, and decreases the anti-oxidant capacity, and subsequently impairs the mitochondrial function in the liver. | Yongqian Duanmu Rihua Cong Shiyu Tao Jing Tian Haibo Dong Yuanshu Zhang Yingdong Ni Ruqian Zhao | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 2 |
| 4 | Effects of maternal treatment of dehydroepiandrosterone (DHEA) on serum lipid profile and hepatic lipid metabolism-related gene expression in embryonic chickens显示文摘 | CHEN Juan TANG Xue ZHANG Yuanshu | 2010 | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology2010，155，4： | 1 |
| 5 | Mechanism of anti-β-adrenoceptor antibody mediated myocardial damage in dilated cardiomyopathy 显示文摘 | Liao Yuhua Cheng Longxian Tu Yuanshu | 1997 | J Tongji Medical University1997，17，： | 1 |
| 6 | Mutant prevention concentrations of fluoroquinolones against Campylobacter je- juni isolated from chicken显示文摘 | Wang L Yuanshu Z Yuhan Z | 2010 | Vet Microbiol2010，144，34： | 1 |
| 7 | A province-scale maize yield estimation method based on TM and Modis time-series interpolation显示文摘 | He Xin Jing Yuanshu Gu Xiaohe | 2010 | Sensor letters2010，8，1： | 1 |
| 8 | Effect of norfloxacin and clonidine on human trabecular meshwork cells in vitro显示文摘 | Wang Qiang Wei Houren Fan Zhgenjun Lu Yuanshu | 1994 | Graefe’s Archive for Clinical and Experimental Ophthalmology1994，，9： | 1 |
| 9 | Postantibiotic effects and postan-tibiotic sub-MIC effects of tilmicosin,erythromycin and tia-mulin on erythromycin-resistant streptococcus suis显示文摘 | Liping Wang Yuanshu Zhang | 2009 | Brazilianjournal of microbiology2009，40，： | 1 |
| 10 | Analysis of Sources of Atmospheric Fine Particulates in Nan'ao County显示文摘Based on on-line single particle aerosol mass spectrometry(SPAMS) with high time resolution,the sources of atmospheric fine particulate matter in Nan'ao County were analyzed.The results showed that during the monitoring period from March 24 to 27 in 2015,the primary source of fine particulate matter in Nao'ao County was motor vehicle exhaust,that is,24.7% of fine particulates were from motor vehicle exhaust,followed by biomass burning(17.2%),fire coal(17.2%),and sea salt(13.3%).The proportions of fire coal and sea salt revealed the peculiarity of sources of atmospheric fine particulate matter in Nan'ao County.Therefore,it is suggested that some measures should be adopted to limit tourist traffic to reduce the adverse impact of motor vehicle exhaust on ambient air quality in Nan'ao County. | Xiao Lianghong Zhang Yufeng Chen Shuchi Xie Yuanshu Guo Yan | 2015 | Meteorological and Environmental Research2015，6，11： | 1 |
| 11 | Identifi-cation and sequence analysis of turnip mosaic virus infection crucif-erous crops in southwest of China 显示文摘 | JIANG Yu WANG Jian-hui YANG Hui XU Mo-yun YUANShu SUN Wen XU Wei-lin XI De-hui UN Hong-hui | 2010 | Journal of Plant Pathology2010，92，： | 1 |
| 12 | Effect of norfloxacin and clonidine on human trabecular meshwork cells in vitro显示文摘 | Wang Qiang Wei Houren Fan Zhgenjun Lu Yuanshu | 1994 | Graefe’s Archive for Clinical and Experimental Ophthalmology1994，，9： | 1 |
| 13 | Evaluating virtual cells and Multistage Flow Shop: A Analytical Approach 显示文摘 | ASOO J VAKHARIA JAYA P MOILY HUANG YUANSHU | 1999 | The International Journal of Flexible Manufacturing Systems1999，，11： | 1 |
| 14 | Infectivity of a genotype 4 hepatitis E virus cDNA clone by intrahepatic inoculation of laboratory rats显示文摘 | Yumin Zhu Xiaoming Yu Yuanshu Zhang Yanyan Ni Fusheng Si Ruisong Yu Shijuan Dong Yaowei Huang Zhen Li | 2013 | Veterinary Microbiology2013，，： | 1 |
| 15 | Effects of maternal treatment of dehydroepiandrosterone(DHEA)on serum lipid profile and hepatic lipid metabolism-related gene expression in embryonic chickens显示文摘 | Chen Juan Tang Xue Zhang Yuanshu | 2010 | Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology2010，155，4： | 1 |
| 16 | Mutant prevention con-centrations of fluoroquinolones against Campylobacter jejuni isolated from chicken显示文摘 | Wang L Yuanshu Z Yuhan Z | 2010 | Vet Microbiol2010，144，34： | 1 |
| 17 | Application of MODTRAN Model in Atmospheric Correction of HJ/CCD Data显示文摘MODTRAN model was used for the atmospheric correction of one HJ-1B / CCD2 image,and the effect of atmospheric correction was evaluated from the changes of spectral characteristics of typical ground objects,the comparison with the MODIS surface reflectance product,and the effect on normalized differential vegetation index( NDVI). The results show that atmospheric correction eliminated the increase effect in visible bands and the absorption in near-infrared band. Atmospheric correction results and the MODIS surface reflectance product with high accuracy were highly consistent in the reflectance of vegetation,water and residents,and the average error of vegetation was 12.8%. According to the comparison of changing characteristics of NDVI before and after atmospheric correction,it could be found that atmospheric correction had corrected NDVI of mixed pixels and made it more reasonable. NDVI of each kind of ground objects improved,among which NDVI of vegetation increased most greatly,which can help differentiate vegetation from other ground objects. In a word,MODTRAN model has a good effect on atmospheric correction of HJ /CCD images. | Xiong Shiwei Hu Shanshan Shen Anyun Yu Linghua Li Weiguo Jing Yuanshu | 2016 | Meteorological and Environmental Research2016，7，6： | 0 |
| 18 | Effect of cefodizime on cytokines expression in mouse neutrophils and its related mechanism显示文摘Objective: To explore the regulating effects of cefodizime on cytokines expression of neutrophil response to Klebsiella pneumoniae (Kle. p) treatment. Methods: We detected the types and expression of cytokines secreted by neutrophils by cDNA array and RT-PCR. We also analyzed the changes of signal transduction in this process by detecting the expression of toll like receptor 4 (TLR4) and the inhibitor factor of κBα (I-κBα) expressed by neutrophils. The activity of NF-κB DNA-binding in neutrophils was measured by electrophoretic mobility shift assay (EMSA). Results: Cefodizime increased the neutrophils production of TNF-α, IL-1β and the mRNA expression of TLR4 in the early stage of Kle. p stimulation in mice, which seemed corresponding to the enhanced NF-κB DNA-binding activity. Conclusion: Cefodizime regulates the cytokines expression of neutrophils through the LPS-TLR4-NF-κB pathway by affecting the expression of TLR4 mRNA and the DNA binding activities of NF-κB in mice with the challenge of Kle. p. | Song Hui Qian Yuanshu Li Guanwu | 2008 | Journal of Medical Colleges of PLA(China)2008，23，4： | 0 |
| 19 | 无形中的有形--秦皇岛阿那亚三期景观设计显示文摘如同2007年龙湖地产奇迹般的创造了北京“龙湖滟澜山”一样,2016年北京天行九州旅游置业开发有限公司在秦皇岛向业界打造了一个现代版的乌托邦社区阿那亚.从2014年第一次接触此项目至今,看到她从无到有的全过程,应该说是当下时代的最经典的产物之一,几乎不可复制. | Yuanshu Insititue of Landscape Planning and Design, Beijing | 2018 | 风景园林2018，25，10： | 0 |
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