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| 1 | Construction and Identification of a Human Liver Specific MicroRNA Eukaryotic Expression Vector显示文摘MiR-122 is one of the non-coding RNAs which showed its effects on the lipo-metablism,virus infection and HCC forming through regulation of liver gene expression.Its eukaryotic expression vector was constructed by using pSuper which was widely applied in the siRNA expression.The precursor of human miR-122 gene was amplified by polymerase chain reaction(PCR)from the human genomic DNA.The positive clones were screened by PCR and restriction enzyme digestion.The new expression vector of miR-122 was named pHsa-m122.PHsa-m122 and its controls were transfected to HepG2 cells.The miR-122 expression activity was evaluated by GFP122i sensor reporter plasmid through fluorescence detection and Western blot.It was shown that the fluorescence intensity of GFP122si and pHsa-m122 co-transfection group was weaker than that of the controls,so the functional activity of expressed miR-122 was detected.When HepG2 cells were co-transfected with HBV1.3 and pHsa-m122 plasmids, the results showed miR-122 may down-regulate the gene expression of HBV.The human liver specific microRNA eukaryotic expression vector of miR-122 was constructed successfully,which may facilitate further study of its function in the development of liver virus infection diseases and HCC. | Shanshan Chen Ming Ni Bing Yu Tingting Lv Mengji Lu Feili Gong | 2007 | Cellular & Molecular Immunology2007，4，6： | 18 |
| 2 | Gene Delivery of SOCS3 Protects Mice from Lethal Endotoxic Shock显示文摘Suppressor of cytokine signaling 3 (SOCS3) was reported as a feedback inhibitor of cytokine receptor signaling by inhibiting the JAK-STAT signal transduction pathway. We sought to test the anti-endotoxic septic shock effect of liposome mediated gene delivery of SOCS3 in a lethal endotoxic shock mouse model. BALB/c mice were injected intraperitoneally with 200 μg pcDNA3.1-SOCS3 cationic liposomes, while pcDNA3.1-IL-10 and empty vector as positive and negative control respectively. Forty-eight hours after gene delivery, mice were challenged with 4 μg of E. coli 0127:B8 LPS and 18 mg D-GalN administered i.p. 90 min later, serum TNF-α level was determined. Survival over the next 48 h was evaluated. Peritoneal macrophages from survival mice were stimulated in vitro with 1 μg/ml LPS for 18 h, and the supernatants were harvested for determination of the amount of TNF-α. We found that gene delivery of SOCS3 significantly increase the mouse survival rate from 27.8 ± 9.6% of control group to 61.1 ± 9.6% (p < 0.01). In comparison with control group (218 ± 13 pg/ml) and sham delivery group (219 ± 22 pg/ml), gene delivery of SOCS3 reduced the level of serum TNF-α (68 ± 9 pg/ml) significantly (p < 0.01). Furthermore, gene delivery of SOCS3 displayed the capacity of prevention of tolerance of peritoneal macrophages to LPS. These findings suggest that gene delivery of SOCS3 mediated by liposome is a promising approach for endotoxic septic shock treatment. Cellular & Molecular Immunology. | Min Fang Hong Dai Guang Yu Feili Gong | 2005 | Cellular & Molecular Immunology2005，2，5： | 5 |
| 3 | Dynamic multiple-fault diagnosis with imperfect tests 显示文摘 | RUAN Sui ZHOU Yunkai YU Feili | 2009 | IEEE Transactions on Systems Man and Cybernetics Part A:Systems and Humans2009，39，6： | 1 |
| 4 | A novel congruent organizational design methodology using group tech-nology and a nested genetic algorithm 显示文摘 | Yu Feili Tu Fang Pattipati K R | 2006 | IEEE Transactions on Systems Man and Cybernetics2006，36，1： | 1 |
| 5 | Evidence for 2-Hexene-1, 6- diyl diradicals accompanying the concerted Diels-Alder cycloaddi- tion of acrylonitrile with Nonpolar 1,3-Diene 显示文摘 | Yu feili Padias A B Hall H K Jr | 1993 | J Org Chem1993，58，： | 1 |
| 6 | A Novel Congruent Organizational Design Methodology Using Group Technology and a Nested Genetic Algorithm显示文摘 | MEIRINA CANDRA RUAN SUI | 2004 | Command and Control Research and Technology Symposium2004，，： | 1 |
| 7 | A novel congruentorganizational design methodology using group technologyand a nested genetic algorithm显示文摘 | YU Feili TU Fang PATTIPATI K R | 2006 | IEEE Transactions onSystems Man and Cybernetics Part A : Systems and Hu-man2006，36，1： | 1 |
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