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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Gastrin,somatostatin,and experimental disturbance of the gastrointestinal tract in rats显示文摘INTRODUCTIONThe field of gastrointestinal hormones has expanded at a dizzying rate[1-4].Gastrointestinal hormones as regulatory peptides that appear to be major components of bodily integration and have important regulatory actions on physioligical function of the gastrointestinal tract .The successful isolation of some gastrointestinal hormones and the development of sensitive methods for their detection have led to the unexpected finding that they also exist in the brain . | Yong Li Yao~1 Bo Xu~2 Wan Dai Zhang~1 Yu Gang Song~1 ~1Institute of Gastrointestinal Diseases,Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2Department of Orthopedics Nanfang Hospital,First Military Medical University,Guangzhou 510515,Guangdong Province,ChinaYong Li Yao graduated from First Military Mcdical University with a master degree in 1998.She now works in the Institute of Gastrointestinal Diseases,Nanfang Hospital as a doctoral candidate majoring gastrointestinal diseases and gastrointestinal hormones,she has published 8 papers. | 2001 | World Journal of Gastroenterology2001，7，3： | 12 |
| 3 | Transretinoic acid inhibits rats gastric epithelial dysplasia induced by N-methyi-N-nitro-N-nitrosoguanidine:influences on cell apoptosis and expression of its regulatory genes显示文摘INTRODUCTIONGastric epithelial dysplasia (GED) hypothetically is a straight-forward concept: dysplastic epithelium replacing the normal gastric epithelium of the stomach [1].In the stomach ,like any other segment of the gut ,it is defined as an unequivocal non-invasive epithelial change[2,3].The observation of gastric dysplasia as a cancerous lesion was recognized over a century ago ,but it is only after the advent of gastroscopy that its clinical significance has been stressed[4-7]. | Ru Tao Cui~1 Gan Cai~2 Zhao Bao Yin~(2*) Yong Cheng~2 Qiu Hong Yang~1 Tao Tian~(3#) ~1Liver Center,Beijing Friendship Hospital Affiliated to CapitalUniversity of Medical Sciences~2Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai China and Department of Pathophysiology.Shanghai Medical University.Shanghai,China ~3Pathological Department,Shanghai Medical University (?)Now working at UCLA,USA ~#Now working at University of Michigan,USADr.Ru Tao Cui graduated and got the bachelor degree from Shandong University of Traditional Chinese Medicine in 1994,got doctor degree from Shanghai University of Traditional Chinese Medicine in 2000,now studying as a postdoc,majoring gastroenterology,having 29 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 4 | Influence of IFNα-2b and BCG on the release of TNF and IL-1 by Kupffer cells in rats with hepatoma显示文摘INTRODUCTIONKupffer cells are residential macrophages in the liver ,which play a critical role in the maintenance of normal liver function and in immunal surveilance of hepatocellular carcinoma (HCC) and other cancers[1].The biological immune modulants have cancers[2].In our previous studies ,the combined use of biological immune modulants showed better dffects . | Xian Yong Bai~1 Xiu Hong Jia~2 Ling Zhong Cheng~3 Yun Di Gu~3 ~1Department of Histology and Embryology,Binzhou Medical College,Binzhou 256603,Shandong Province,China ~2Departrnent of Pediatrics,Binzhou Medical College,Binzhou 256603,Shandong Province,China ~3Department of Histology and Embryology,Shanghai Medical University,Shanghai 200032,ChinaDr.Xian Yong Bai graduated from the Shanghai Medical University as a postgraduate in 1995,Professor of Histology and Embryology,specialized in hepatic tumor immunology,having 36 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 7 |
| 5 | Percutaneous transcatheter assessment of the left main coronary artery: current status and future directions显示文摘 | Bing R Yong AS Lowe HC | 2015 | JACC Cardiovasc Interv2015，8，12： | 1 |
| 6 | Cardiac troponin-T and the pre- diction of acute and long-term mortality 'after acute pulmonary em- bolism显示文摘 | Ng AC Yong AS Chow V | 2011 | International Journal of Cardiology Available online2011，24，： | 1 |
| 7 | Ex vivo characterization of polyclonal memory CD8+ T-cell responses to PRAME-specific peptides in patients with acute lymphoblastic leukemia and acute and chronic myeloid leukemia显示文摘 | Rezvani K Yong AS Tawab A | | 0，，： | 1 |
| 8 | Circulating levels of soluble EMMPRIN (CD147) correlate with levels of soluble glycoprotein VI in human plasma显示文摘 | Pennings GJ Yong AS Wong C | 2014 | Platelets2014，25，8： | 1 |
| 9 | Repeated PR1 and WT1 peptide vaccination in Montanide-adjuvant fails to induce sustained high-avidity,epitope-specific CD8+ T cells in myeloid malignancies显示文摘 | Rezvani K Yong AS Mielke S | | 0，，： | 1 |
| 10 | Monoclonal antibody targe-ting of IL - 3 receptor a with CSL362 effectively depletes CMLprogenitor and stem cells 显示文摘 | Nievergall E Ramshaw HS Yong AS | 2014 | Blood2014，123，8： | 1 |
| 11 | Uremic pruritus is improved by gabapentin 显示文摘 | Yong AS Lee KY | 2014 | Int J Dermatol2014，53，9： | 1 |
| 12 | Association between BMI-1 expression,acute graft-versus-host disease,and outcome following allogeneic stem cell transplantation from HLA-identical siblings in chronic myeloid leukemia显示文摘 | Mohty M Szydlo RM Yong AS | | Blood0，，： | 1 |
| 13 | Expression of EMMPRIN (CD147) on circulating platelets in vivo显示文摘 | Pennings GJ Yong AS Kritharides L | 2010 | JThromb Haemost2010，8，3： | 1 |
| 14 | The polycomb group BMI1 gene is a molecular marker for predicting prognosis of chronic myeloid leukemia显示文摘 | Mohty M Yong AS Szydlo RM | 2007 | Blood2007，110，1： | 1 |
| 15 | The polycombgroup BMI1 gene is a molecular marker for predictingprognosis of chronic myeloid leukemia 显示文摘 | Mohty M Yong AS Szydlo RM | 2007 | Blood2007，110，1： | 1 |
| 16 | Acute pulmonary embol- ism in individuals aged 80 and older显示文摘 | Moutzouris JP Chow V Yong AS | 2014 | J Am Geriatr Soc2014，，62： | 1 |
| 17 | The index of microcirculatory resistance predicts myocardial infarction related to percutaneous coronary intervention显示文摘 | Ng MK Yong AS Ho M | 2012 | Circ Cardiovasc Interv2012，5，4： | 1 |
| 18 | Prognostic value of the index of microcirculatory resistance measured after primary percutaneous coronary intervention显示文摘 | Fearon WF Low AF Yong AS | 2013 | Circulation2013，127，24： | 1 |
| 19 | Prognostie impact of the Charlson comorbidity index on mortality following acute pulmonary embolism显示文摘 | Ng AC Chow V Yong AS | 2013 | Respiration2013，85，5： | 1 |
| 20 | Prognostic Impact of the Charl- son Comorbidity Index on Mortality following Acute Pulmonary Em- bolism显示文摘 | Ng AC Chow V Yong AS | 2013 | Respiration2013，85，5： | 1 |
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