|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A panel of monoclonal antibodies against the prion protein proves that there is no prion protein in human pancreatic ductal epithelial cells显示文摘Prion diseases are a group of neurodegenerative diseases that are fatal. The study of these unique diseases in China is hampered by a lack of resources. Amongst the most important resources for biological study are monoclonal antibodies. Here, we characterize a panel of monoclonal antibodies specific for cellular prion protein by enzyme-linked immunosorbent assay(ELISA), immunofluorescent staining, flow cytometry, and western blotting. We identify several antibodies that can be used for specific applications and we demonstrate that there is no prion protein expression in human pancreatic ductal epithelial cells(HPDC). | Liheng Yang Yan Zhang Lipeng Hu Ying Zhu Man-Sun Sy Chaoyang Li | 2014 | Virologica Sinica2014，29，4： | 3 |
| 2 | Malaria over-diagnosis in Cameroon:diagnostic accuracy of Fluorescence and Staining Technologies(FAST)Malaria Stain and LED microscopy versus Giemsa and bright field microscopy validated by polymerase chain reaction显示文摘Background:Malaria is a major world health issue and its continued burden is due,in part,to difficulties in the diagnosis of the illness.The World Health Organization recommends confirmatory testing using microscopy-based techniques or rapid diagnostic tests(RDT)for all cases of suspected malaria.In regions where Plasmodium species are indigenous,there are multiple etiologies of fever leading to misdiagnoses,especially in populations where HIV is prevalent and children.To determine the frequency of malaria infection in febrile patients over an 8-month period at the Regional Hospital in Bamenda,Cameroon,we evaluated the clinical efficacy of the Flourescence and Staining Technology(FAST)Malaria stain and ParaLens AdvanceTM microscopy system(FM)and compared it with conventional bright field microscopy and Giemsa stain(GS).Methods:Peripheral blood samples from 522 patients with a clinical diagnosis of“suspected malaria”were evaluated using GS and FM methods.A nested PCR assay was the gold standard to compare the two methods.PCR positivity,sensitivity,specificity,positive predictive value(PPV),and negative predictive value(NPV)were determined.Results:Four hundred ninety nine samples were included in the final analysis.Of these,30 were positive via PCR(6.01%)with a mean PPV of 19.62%and 27.99%for GS and FM,respectively.The mean NPV was 95.01%and 95.28%for GS and FM,respectively.Sensitivity was 26.67%in both groups and specificity was 92.78%and 96.21%for GS and FM,respectively.An increased level of diagnostic discrepancy was observed between technicians based upon skill level using GS,which was not seen with FM.Conclusions:The frequency of malarial infections confirmed via PCR among patients presenting with fever and other symptoms of malaria was dramatically lower than that anticipated based upon physicians’clinical suspicions.A correlation between technician skill and accuracy of malaria diagnosis using GS was observed that was less pronounced using FM.Additionally,FM increased the specificity and improved the PPV,suggesting this relatively low cost approach could be useful in resource-limited environments.Anecdotally,physicians were reluctant to not treat all patients symptomatically before results were known and in spite of a negative microscopic diagnosis,highlighting the need for further physician education to avoid this practice of overtreatment.A larger study in an area with a known high prevalence is being planned to compare the two microscopy methods against available RDTs. | Sean M.Parsel Steven A.Gustafson Edward Friedlander Alexander A.Shnyra Aderosoye J.Adegbulu Ying Liu Nicole M.Parrish Syed A.Jamal Eve Lofthus Leo Ayuk Charles Awasom Carolyn J.Henry Carole P.McArthur | 2017 | Infectious Diseases of Poverty2017，6，1： | 2 |
| 3 | Ultrasound of malignant cervical lymph nodes显示文摘 | Ahuja AT Ying M Ho SY | 2008 | Cancer Imaging2008，8，1： | 1 |
| 4 | A homodimer of the β-subunit of inhibin A stimulates the secretion of pituitary follicle stimulating hormone显示文摘 | Ling N Ying SY Ueno N | 1986 | Biochem Biophys Res Commum1986，138，： | 1 |
| 5 | Endoscopic-assisted subcutaneous mastectomy and axillary dissection with immediate mammary prosthesis reconstruction for early breast cancer显示文摘 | Ho WS Ying SY Chan AC | 2002 | Surg Endosc2002，16，2： | 1 |
| 6 | An epidemiological study of 1063 hospitalized burn patients in a tertiary burns centre in Hong Kong显示文摘 | Ho WS Ying SY | 2001 | Burns2001，27，2： | 1 |
| 7 | Inhibin production and secretion in human placental cells cultured in vitro显示文摘 | Qu JP Ying SY Thomas K | 1992 | Obstet Gynecol1992，79，5： | 1 |
| 8 | Treatment of port wine stainswith intense pulsed light:a prospective study显示文摘 | HoWS Ying SY Chan PC | 2004 | DermatolSurg2004，30，6： | 1 |
| 9 | The microRNA (miRNA) : overview of the RNA genes that modulate gene function 显示文摘 | Ying SY Chang DC Lin SL | 2008 | Mol Biotechnol2008，38，3： | 1 |
| 10 | A novel mRNA-cDNA interference phenomenon for silencing bcl-2 expression in human LNCaP cells显示文摘 | Lin SL Chuong CM Ying SY | 2001 | Biochem Biophys Res Commun2001，281，3： | 1 |
| 11 | A 10-year study of the changes in the pattern and treatment of 6493 fractures显示文摘 | Cheng JC Ng BK Ying SY | 1999 | J Pediatr Orthop1999，19，3： | 1 |
| 12 | Incidental Receipt of Cardiac Medications and Survival Outcomes Among Patients With Stage III Non–Small-Cell Lung Cancer After Definitive Radiotherapy显示文摘 | Hongmei Wang Zhongxing Liao Yan Zhuang Ying Liu Lawrence B. Levy Ting Xu Syed Wamique Yusuf Daniel R. Gomez | 2014 | Clinical Lung Cancer2014，，： | 1 |
| 13 | Transcriptional control of Shh/Ptc1 signaling in embryonic development显示文摘 | Lin SL Chang S J Ying SY | 2006 | Gene2006，367，： | 1 |
| 14 | Pituitary FSH is released by a heterodimer of the beta-subunits from the two forms ofinhibin显示文摘 | Ying SY Vaughan J | 1986 | Nature1986，321，6072： | 1 |
| 15 | China leads in ramie显示文摘 | Sy Liu Ying | 1990 | Textile Asia1990，，3： | 1 |
| 16 | Endoscopic-assisted subcutaneous mastectomy and axillary dissection with immediate mammary prosthesis reconstruction for early breast cancer显示文摘 | Ho WS Ying SY Chan AC | 2002 | Surg Endosc2002，16，2： | 1 |
| 17 | Prospective Study on the Treatment of Postburn Hyperpigmentation by Intense Pulsed Light 显示文摘 | Ho WS Chan HH Ying SY | 2003 | Lasers Surg Med2003，32，： | 1 |
| 18 | The microRNA (miRNA):overview of the RNA genes that modulate gene function显示文摘 | Ying SY Chang DC Lin SL | 2008 | Mol Biotechnol2008，38，3： | 1 |
| 19 | Intron-mediated RNA interference and microRNA biogenesis显示文摘 | Ying SY Lin SL | 2009 | Methods Mol Biol2009，487，： | 1 |
| 20 | Recurrence of nevus of Ota after successful treatment with Q-switched lasers 显示文摘 | Chan HH Leung RS Ying SY | 2000 | Arch Dermatol2000，136，9： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员