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| 1 | Single-cell RNA sequencing reveals Nestin+active neural stem cells outside the central canal after spinal cord injury显示文摘Neural stem cells(NSCs)in the spinal cord hold great potential for repair after spinal cord injury(SCI).The ependyma in the central canal(CC)region has been considered as the NSCs source in the spinal cord.However,the ependyma function as NSCs after SCI is still under debate.We used Nestin as a marker to isolate potential NSCs and their immediate progeny,and characterized the cells before and after SCI by single-cell RNA-sequencing(scRNA-seq).We identified two subgroups of NSCs:the subgroup located within the CC cannot prime to active NSCs after SCI,while the subgroup located outside the CC were activated and exhibited the active NSCs properties after SCI.We demonstrated the comprehensive dynamic transcriptome of NSCs from quiescent to active NSCs after SCI.This study reveals that Nestin+cells outside CC were NSCs that activated upon SCI and may thus serve as endogenous NSCs for regenerative treatment of SCI in the future. | Muya Shu Xiaoyu Xue Hu Nie Xianming Wu Minghan Sun Lianyong Qiao Xing Li Bai Xu Zhifeng Xiao Yannan Zhao Yongheng Fan Bing Chen Jixiang Zhang Ya Shi Yaming Yang Falong Lu Jianwu Dai | 2022 | Science China(Life Sciences)2022，65，2： | 2 |
| 2 | Yield photosynthesis and leaf anatomy of maize in inter-and mono-cropping systems at varying plant densities显示文摘Increasing plant density can increase cereal crop yields. However, the physiological and anatomical mechanisms of grain yield increase at high plant densities in maize-based intercropping systems are not well understood. A two-year field experiment was conducted in 2018 and 2019 to investigate grain yield, photosynthetic characteristics, stomatal traits, and leaf anatomy of maize plants in an intercropping system with high plant densities. Two cropping patterns(monocropping and intercropping) and three plant densities(D1, 78,000 plants ha^(-1);D2, 103,500 plants ha^(-1);D3, 129,000 plants ha;were arranged in a randomized block design. Increasing maize plant density significantly increased maize yield, and intercropping gave a significant yield advantage over monocropping under the same plant density. Intercropping combined with high plant density increased the leaf area and SPAD value of maize,increasing the photosynthesis rates after the harvest of pea. At the twelfth leaf stage, the stomatal density and stomatal area of intercrops combined with medium plant density increased by respectively 10.5%and 18.4% relative to their values for the corresponding density of monocrops. Although leaf thickness of maize was reduced by increasing plant density, the chloroplast number and grana lamella number were higher in intercropping than in monocropping under different plant densities. These positive changes in leaf anatomy resulted in increased photosynthesis, suggesting a physiological basis for the increase in grain yield. | Hongwei Yang Qiang Chai Wen Yin Falong Hu Anzhen Qin Zhilong Fan Aizhong Yu Cai Zhao Hong Fan | 2022 | The Crop Journal2022，10，3： | 2 |
| 3 | Short hairpin RNA targeting NP mRNA inhibiting Newcastle disease virus production and other viral structural mRNA transcription显示文摘 | Yue Hua Deng Shu Yang Falong Li Dingfei Fu Anjing Yang Fan Tang Cheng | 2009 | Virus gene2009，38，1： | 1 |
| 4 | Adaptive Null-Forming Scheme in Digital Hearing Aids显示文摘 | LUO Falong YANG Jun Pavlovic C Nehorai A | 2002 | IEEE Transactions on Signal Processing2002，50，7： | 1 |
| 5 | Adaptive null-forming scheme in digital hearing aids 显示文摘 | LUO Falong YANG Jun | 2002 | IEEE Transactions on Signal Pro- cessing2002，50，7： | 1 |
| 6 | Development of quantitative real-time polymerase chain reaction for duck eateritiz virus DNA显示文摘 | Falong Yang | 2005 | Avian Diseases2005，49，3： | 1 |
| 7 | Development of quantitativereal-time polymerase chain reaction for duck enteritis virus DNA显示文摘 | FaLong Yang WenXiang Jia Hua Yue | 2005 | Avain Disease2005，49，3： | 1 |
| 8 | Adaptive null-forming scheme in digital hearing aids 显示文摘 | LUO Falong YANG Jun Pavlovic C | 2002 | IEEE Trans on Acoust Speech and Signal Processing2002，50，： | 1 |
| 9 | Genome sequence of Mycoplasma ovipneumoniae strain SCO1 显示文摘 | YANG Falong TANG Cheng WANG Yong | 2011 | J Bacteriol2011，193，18： | 1 |
| 10 | Adaptive null-forming scheme in digital hearing aids显示文摘 | LUO Falong YANG Jun Pavlovic C | 2002 | IEEE Transactions on Signal Processing2002，50，7： | 1 |
| 11 | Radiation-induced sarcoma of head and neck: 50 years of experience at a single institution in an endemic area of nasopharyngeal carcinoma in China显示文摘 | Zhengbo Wei Ying Xie Jian Xu Yuan Luo Falong Chen Yunli Yang Qifang Huang Anzhou Tang Guangwu Huang | 2012 | Medical Oncology2012，，2： | 1 |
| 12 | KLF4 facilitates chromatin accessibility remodeling in porcine early embryos显示文摘Chromatin accessibility remodeling driven by pioneer factors is critical for the development of early embryos.Current studies have illustrated several pioneer factors as being important for agricultural animals,but what are the pioneer factors and how the pioneer factors remodel the chromatin accessibility in porcine early embryos is not clear.By employing low-input DNase-seq(liDNase-seq),we profiled the landscapes of chromatin accessibility in porcine early embryos and uncovered a unique chromatin accessibility reprogramming pattern during porcine preimplantation development.Our data revealed that KLF4 played critical roles in remodeling chromatin accessibility in porcine early embryos.Knocking down of KLF4 led to the reduction of chromatin accessibility in early embryos,whereas KLF4 overexpression promoted the chromatin openness in porcine blastocysts.Furthermore,KLF4 deficiency resulted in mitochondrial dysfunction and developmental failure of porcine embryos.In addition,we found that overexpression of KLF4 in blastocysts promoted lipid droplet accumulation,whereas knockdown of KLF4 disrupted this process.Taken together,our study revealed the chromatin accessibility dynamics and identified KLF4 as a key regulator in chromatin accessibility and cellular metabolism during porcine preimplantation embryo development. | Wei Zhu Guowei Bu Ruifeng Hu Jixiang Zhang Lianyong Qiao Kai Zhou Tingting Wang Qiao Li Jingjing Zhang Linhui Wu Yali Xie Taotao Hu Shichun Yang Jiaqi Guan Xiaoyu Chu Juanjuan Shi Xia Zhang Falong Lu Xin Liu Yi-Liang Miao | 2024 | Science China(Life Sciences)2024，67，1： | 0 |
| 13 | Transplantation of neural stem progenitor cells from different sources for severe spinal cord injury repair in rat显示文摘Neural stem progenitor cell(NSPC)transplantation has been regarded as a promising therapeutic method for spinal cord injury(SCI)repair.However,different NSPCs may have different therapeutic effects,and it is therefore important to identify the optimal NSPC type.In our study,we compared the transcriptomes of human fetal brain-derived NSPCs(BNSPCs),spinal cord-derived NSPCs(SCNSPCs)and H9 embryonic stem-cell derived NSPCs(H9-NSPCs)in vitro and subsequently we transplanted each NSPC type on a collagen scaffold into a T8-9 complete SCI rat model in vivo.In vitro data showed that SCNSPCs had more highly expressed genes involved in nerve-related functions than the other two cell types.In vivo,compared with BNSPCs and H9-NSPCs,SCNSPCs exhibited the best therapeutic effects;in fact,SCNSPCs facilitated electrophysiological and hindlimb functional recovery.This study demonstrates that SCNSPCs may be an appropriate candidate cell type for SCI repair,which is of great clinical significance. | Bai Xu Man Yin Yaming Yang Yunlong Zou Wenbin Liu Lianyong Qiao Jixiang Zhang Zhan Wang Yayu Wu He Shen Minghan Sun Weiyuan Liu Weiwei Xue Yongheng Fan Qi Zhang Bing Chen Xianming Wu Ya Shi Falong Lu Yannan Zhao Zhifeng Xiao Jianwu Dai | 2023 | Bioactive Materials2023，，5： | 0 |
| 14 | shRNA-triggered RNAi inhibits expression of NDV NP gene in chicken embryo fibroblast显示文摘RNA interference(RNAi)technology is a powerful tool for identifying gene functions.Chicken embryo fibroblast(CEF)is an ideal model for studying the interaction between avian viruses and their hosts.To establish a methodological platform for RNAi studies in CEF,three plasmid vectors expressing short hairpin RNAs(shRNAs)targeted against the Newcastle disease virus(NDV)NP gene were constructed.One of them,ndv1,was proven effective on blocking viral replication in CEF and chicken embryos.Four hours prior to infection with NDV,the CEF was transfected with the plasmids by Silent-fect.An unrelated shRNA sequence(HK)was used in mock transfection.The expression of a potent shRNA resulted in up to 2.3,21.1 and 9.8 fold decreases in NP gene expression at 3,6 and 9 h post infection in CEF,respectively.The ndv1 was able to completely inhibit the replication of the virus in CEF within 48 post infection.Furthermore,the pathological changes in CEF caused by NDV were delayed,and the degree of pathological changes was lighter compared with the mock transfection in the presence of ndv1.When the complex of shRNASilent-fect and NDV was co-injected into the allantoic cavity of 10-day-old embryonated eggs with 10^(5) or 10^(6) ELD50 NDV,NDV replication was decreased by 94.14% and 62.15% after 17 h,respectively.These findings suggest that the newly synthesized NP protein is critical for NDV transcription and replication and provide a basis for identifying the functions of viral genes and screening for effective siRNAs against viruses in CEF and chicken embryo by RNAi. | Hua YUE Dingfei LI Anjing FU Li MA Falong YANG Cheng TANG | 2008 | Frontiers in Biology2008，3，4： | 0 |
| 15 | First identification of a novel Aichivirus D in goats with diarrhea显示文摘Kobuvirus comprises 6 officially recognized species,namely Aichivirus A-F,and can be further divided into 20 genotypes through VP1 gene phylogenetic analysis(http://gffzzc5675cdb024b4598sf6f05qux6uxf66cf.ffgz.tsg.suse.edu.cn/report/chapter/picornaviridae/picornaviridae/kobuvirus).Aichivirus A in human,Aichivirus B in bovine,Aichivirus C in porcine and caprine,Aichivirus D in yak have been proved to be associated with diarrhea(Chen Y S et al.2013;Yang et al.2015;Zhu et al.2016;Zhai et al.2017;Wang et al.2020;Abi et al.2022;Yan et al.2023). | Chen Yang Keha-mo Abi Hua Yue Falong Yang Cheng Tang | 2024 | Journal of Integrative Agriculture2024，23，4： | 0 |
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