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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | A new method for judging the computational stability of the difference schemes of nonlinear evolution equations显示文摘For the non-conservative difference schemes of nonlinear evolution equations with aperiodic boundary conditions, taken one-dimensional nonlinear advection equation as an example, a new method for judging the computational stability is given. It is proved to be practical and effective through several numerical examples. The stability criteria obtained by this method are really the necessary conditions of computational stability. | LIN Wantao Jl Zhongzhen WANG Bin YANG Xiaozhong | 2000 | Chinese Science Bulletin2000，45，15： | 18 |
| 3 | 基于流行病学、空间、基因组学数据的中国上海内部人口流动与结核病传播动力学研究显示文摘中国国内人口大规模从农村向城市流动,为结核病控制工作带来了新的挑战。2018年7月的《The Lancet. Infectious diseases》刊载了一项研究,旨在将基因组数据、空间数据和流行病学数据结合起来,描述有大量流动人口的城市环境中结核病的传播动力学特征。研究者们针对上海松江地区的培养阳性结核分枝杆菌开展了一项基于人群的研究。采用全基因组测序法,对具有相同"数目可变串联重复序列(VNTR)"组合的结核分枝杆菌表观基因簇进行了区分,并分析了居住距离与基因学成簇的结合分枝杆菌感染风险之间的关系。 | Yang C Lu L Warren JL | 2018 | 疾病监测2018，33，8： | 17 |
| 4 | ANOMALOUS BEHAVIOR REVISITED:DYNAMIC RESPONSE OF ELASTIC-PLASTIC STRUCTURES显示文摘Based on the minimum principle of acceleration in the elastic-plastic continua under fi-nite deformation,the dynamic response of an elastic-perfectly plastic pin-ended beam subjected to rect-angular impulse loading is studied with the help of a numerical approach.The calculated results once a-gain show the anomalous behavior of the beam during its response process,which was previously foundin[1].By carefully analyzing the instantaneous distribution of the bending moment,the membraneforce,the curvature and displacement during the response process,it is concluded that the interactiveeffect between the geometry and materials nonlinearities of the structure is the key reason for leading tothe anomalous behavior.This will be helpful for clarifying some misunderstandings in explaining theproblem before. | Xi, F Yang, JL Li, ZL | 1998 | Acta Mechanica Solida Sinica1998，11，4： | 7 |
| 5 | 自取样本高危型HPV分型检测的子宫颈癌初筛及其联合细胞学或病毒载量的二次筛查方案的评价显示文摘目的评价阴道自取样本高危型HPV(HR-HPV)分型检测用于子宫颈癌初筛的筛查效率,并探讨以不同HR-HPV亚型组合联合医生取样本的细胞学检查或HR-HPV病毒载量检测对自取样本HR-HPV阳性者进行二次筛查的筛查效率。方法本研究的数据来自2009年4月至2010年4月深圳市子宫颈癌筛查项目Ⅱ(SHENCCAST-Ⅱ)数据库,收集其中有自取样本的基质辅助激光解吸电离-飞行时间质谱分析(MALDI-TOF-MS)技术进行的HR-HPV分型检测结果以及医生取样本的第2代杂交捕获技术(HC-Ⅱ)检测的HR-HPV病毒载量结果、子宫颈细胞学检查结果的妇女共8556例,转诊阴道镜检查者均有活检组织病理检查结果。分析自取样本HR-HPV分型检测作为子宫颈癌初筛方案(即方案1)时,基于其不同HR-HPV亚型组合[包括5个亚方案,即1a:HPV 16和(或)18型(HPV 16/18型)阳性;1b:HPV 16/18/58型阳性;1c:HPV 16/18/58/31/33型阳性;1d:HPV 16/18/58/31/33/52型阳性;1e:所有14种HR-HPV亚型中任一亚型阳性]的阴道镜转诊方案检出子宫颈上皮内瘤变(CIN)Ⅱ及以上病变(CINⅡ+)、CINⅢ及以上病变(CINⅢ+)的敏感度与特异度;并以自取样本HR-HPV初筛阳性者即以方案1中的各亚方案为基础,对比分析其联合医生取样本的细胞学检查(即方案2,包括2a、2b、2c、2d、2e共5个亚方案)或HR-HPV病毒载量检测(即方案3,包括3a、3b、3c、3d共4个亚方案)作为分流指标的二次筛查方案的筛查效率。结果(1)本研究纳入的8556例妇女的年龄为(38.9±7.9)岁,自取样本检测的HR-HPV阳性率为13.77%(1178/8556),其中HPV 16/18型、HPV 16/18/58型、HPV 16/18/58/31/33型和HPV 16/18/58/31/33/52型组合的阳性率分别为3.16%(270/8556)、5.14%(440/8556)、6.66%(570/8556)和9.81%(839/8556)。医生取样本的HR-HPV病毒载量≥10相对光单位/临床阈值(RLU/CO)者占8.87%(759/8556),细胞学结果≥未明确诊断意义的不典型鳞状上皮细胞(ASCUS)者占12.05%(1031/8556)。(2)方案1中,所有14种HR-HPV亚型中任一亚型阳性者(即方案1e)行阴道镜检查对检出CINⅡ+、CINⅢ+的敏感度在所有方案(包括3个方案共14个亚方案)中最高(分别为92.70%、94.33%),但是特异度和阳性预测值(PPV)在所有方案中最低(特异度分别为88.44%、87.58%,PPV分别为18.34%、11.29%),且阴道镜检查率在所有方案中最高(为13.77%)。方案1的其他亚方案(即方案1a、1b、1c、1d)中,检出CINⅡ+、CINⅢ+的特异度方案1a最高,分别为97.92%、97.69%,其他亚方案也较高,均达90%以上;但敏感度方案1d最高(分别为88.41%、92.20%)。(3)HPV 16/18型阳性者直接行阴道镜检查(即方案1a),非HPV 16/18型阳性者行医生取样本细胞学检查或HR-HPV病毒载量检测,细胞学结果达到阈值(≥ASCUS,即方案2a)或病毒载量达到阈值(≥10 RUL/CO,即方案3a)者行阴道镜检查,其阴道镜转诊率低,而筛查CINⅡ+、CINⅢ+的敏感度和特异度则较高。若在此两个二次筛查方案基础上,首先根据自取样本HR-HPV分型检测结果,依次增加另外4种HR-HPV亚型(即HPV 58、31、33和52型)阳性者行阴道镜检查,再以细胞学检查或病毒载量检测结果进行二次分流,筛查CINⅡ+、CINⅢ+的敏感度也相应提高。结论以自取样本HR-HPV分型检测为子宫颈癌初筛方案,以及其联合细胞学检查或病毒载量检测进行二次筛查的方案,可以在筛查CINⅡ+、CINⅢ+的敏感度、特异度以及阴道镜检查率之间获得较好的平衡;将HPV 58、31、33、52型纳入HPV 16/18型阳性的初筛分流指标,并联合细胞学检查或病毒载量检测的序贯二次筛查,可进一步提高筛查效率。 | 郭春磊 罗红学 王纯 渠新风 Yang Bin Belinson JL 杜辉 吴瑞芳 | 2021 | 中华妇产科杂志2021，56，4： | 6 |
| 6 | Rapid health transition in China, 1990–2010: findings from the Global Burden of Disease Study 2010显示文摘 | Gonghuan Yang Yu Wang Yixin Zeng George F Gao Xiaofeng Liang Maigeng Zhou Xia Wan Shicheng Yu Yuhong Jiang Mohsen Naghavi Theo Vos Haidong Wang Alan D Lopez Christopher JL Murray | 2013 | The Lancet . 2013 (9882)2013，，9882： | 4 |
| 7 | Rapid health transition in China, 1990–2010: findings from the Global Burden of Disease Study 2010显示文摘 | Gonghuan Yang Yu Wang Yixin Zeng George F Gao Xiaofeng Liang Maigeng Zhou Xia Wan Shicheng Yu Yuhong Jiang Mohsen Naghavi Theo Vos Haidong Wang Alan D Lopez Christopher JL Murray | 2013 | The Lancet2013，，9882： | 3 |
| 8 | Rapid health transition in China, 1990–2010: findings from the Global Burden of Disease Study 2010显示文摘 | Gonghuan Yang Yu Wang Yixin Zeng George F Gao Xiaofeng Liang Maigeng Zhou Xia Wan Shicheng Yu Yuhong Jiang Mohsen Naghavi Theo Vos Haidong Wang Alan D Lopez Christopher JL Murray | 2013 | 2013 (9882)2013，，9882： | 3 |
| 9 | Hypoxia-reoxygenation-induced apoptosis in cultured adult rat myocytes and the protective effect of platelets and transforming growth factor-beta ( 1 ) 显示文摘 | Yang BC Zander DS Mehta JL | 1999 | J Pharmacol Exp Ther1999，291，2： | 2 |
| 10 | 益生菌抑制自发性高血压大鼠的菌群失调和血压升高:短链脂肪酸的作用显示文摘该研究旨在评估益生菌短双歧杆菌CECT7263(BFM)及发酵乳酸杆菌CECT5716(LC40)、短链脂肪酸丁酸盐及醋酸盐对自发性高血压大鼠(spontaneously hypertensive rat,SHR)心血管的作用。方法和结果:5周龄的Wistar Kyoto大鼠(WKY)10只和周龄匹配的SHR 50只随机分为6组:对照WKY、对照SHR、BFM干预的SHR、LC40干预的SHR、丁酸盐干预的SHR和醋酸盐干预的SHR。 | 袁源(译) 练桂丽(审校) Robles-Vera I Toral M de la Visitación N Sánchez M Gómez-Guzmán M Romero M Yang T Izquierdo-Garcia JL Jiménez R Ruiz-Cabello J Guerra-Hernández E Raizada MK Pérez-Vizcaíno F Duarte J | 2020 | 中华高血压杂志2020，28，9： | 2 |
| 11 | Single oxygen is the major species participating in the induction of DNA strand breakage and 8-hydroxydeoxyguanosine adduct by lead acetate显示文摘 | Yang JL Wang LC Chang CY | 1999 | Environmental and molecular mutagenesis1999，33，： | 2 |
| 12 | Amelioration of collagen-induced arthritis by CD95 (Apo-1/Fas)-ligand gene transfer 显示文摘 | Yang Y Horton JL | 1997 | J Clin Invest1997，100，8： | 1 |
| 13 | Different effects of post-conditioning on myocardial no-reflow in the normal and hypercholesterolemic mini-swines显示文摘 | Zhao JL Yang YJ You SJ | 2007 | Microvasc Res2007，73，2： | 1 |
| 14 | Diminished hepatic response to fasting/refeeding and liver X receptor agonists in mice with selective deficiency of sterol regulatory element-binding protein-1c显示文摘 | Liang G Yang J Horton JD Hammer RE Goldstein JL Brown MS | 2002 | J Biol Chem2002，277，11： | 1 |
| 15 | Singlet oxygen is the major species participating in the induction of DNA strand breakage and 8-hydroxydeoxyguanosine adduct by lead acetate 显示文摘 | YANG JL WANG LC CHANG CY | 1999 | Environ Mol Mutagen1999，33，3： | 1 |
| 16 | Shoulder kinematic features using arm elevation and rotation tests for classifying patients with frozen shoulder syndrome who respond to physical therapy显示文摘 | Yang JL Chang CW Chen SY | 2008 | Manual Therapy2008，13，6： | 1 |
| 17 | Current clinical regulation of PDK/PTEN/Akt/mTOR signalling in treatment of human cancer 显示文摘 | Lim HJ Crowe P Yang JL | 2015 | JCancer Res Clin Oncol2015，141，4： | 1 |
| 18 | Cyclin B1 proteolysis via p38 MAPK signaling participates in G2 checkpoint elicited by arsenite显示文摘 | Li JP Yang JL | 2007 | J Cell Physiol2007，212，2： | 1 |
| 19 | Genetic markers of survival and liver recurrence after resection of liver metastases from colorectal cancer显示文摘 | Crowe PJ Yang JL Berney CR | 2001 | World J Surg2001，25，8： | 1 |
| 20 | Different Effects of Tirofiban and Aspirin plus Clopidogrel on Myocardial No-Reflow in a Mini-swine Model of Acute Myocardial Infarction and Reperfusion 显示文摘 | Yang YJ Zhao JL You SJ | 2006 | Heart2006，92，8： | 1 |
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