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| 1 | Glycer-AGEs-RAGE signaling enhances the angiogenic potential of hepatocellular carcinoma by upregulating VEGF expression显示文摘AIM:To investigate the effect of glyceraldehyde-derived advanced glycation end-products(Glycer-AGEs) on hepatocellular carcinoma(HCC)cells.METHODS:Two HCC cell lines(Hep3B and HepG2 cells)and human umbilical vein endothelial cells(HUVEC)were used.Cell viability was determined using the WST-8 assay.Western blotting,enzyme linked immunosorbent assay,and real-time reverse transcriptionpolymerase chain reactions were used to detect protein and mRNA.Angiogenesis was evaluated by assessing the proliferation,migration,and tube formation of HUVEC.RESULTS:The receptor for AGEs(RAGE)protein was detected in Hep3B and HepG2 cells.HepG2 cells werenot affected by the addition of Glycer-AGEs.GlycerAGEs markedly increased vascular endothelial growth factor(VEGF)mRNA and protein expression,which is one of the most potent angiogenic factors.Compared with the control unglycated bovine serum albumin(BSA) treatment,VEGF mRNA expression levels induced by the Glycer-AGEs treatment were 1.00±0.10 vs 1.92 ±0.09(P<0.01).Similarly,protein expression levels induced by the Glycer-AGEs treatment were 1.63±0.04 ng/mL vs 2.28±0.17 ng/mL for the 24 h treatment and 3.36±0.10 ng/mL vs 4.79±0.31 ng/mL for the 48 h treatment,respectively(P<0.01).Furthermore,compared with the effect of the control unglycated BSA-treated conditioned medium,the Glycer-AGEstreated conditioned medium significantly increased the proliferation,migration,and tube formation of HUVEC,with values of 122.4%±9.0%vs 144.5%±11.3%for cell viability,4.29±1.53 vs 6.78±1.84 for migration indices,and 71.0±7.5 vs 112.4±8.0 for the number of branching points,respectively(P<0.01).CONCLUSION:These results suggest that Glycer-AGEs-RAGE signaling enhances the angiogenic potential of HCC cells by upregulating VEGF expression. | Junichi Takino Shoichi Yamagishi Masayoshi Takeuchi | 2012 | World Journal of Gastroenterology2012，18，15： | 27 |
| 2 | Differential mucin phenotypes and their significance in a variation of colorectal carcinoma显示文摘AIM: To investigate mucin expression profiles in colorectal carcinoma (CRC) histological subtypes with regard to clinicopathologic variables and prognosis. METHODS: Mucin (MUC)2 and MUC5AC expressions were assessed by immunohistochemistry for a total of 250 CRC cases that underwent surgical resection. CRCs included 63 well-to-moderately differentiated adenocar-cinomas (WMDAs), 91 poorly differentiated adenocarcinomas (PDAs), 81 mucinous adenocarcinoma (MUAs), and 15 signet-ring cell carcinomas (SRCCs). MUC2 and MUC5AC were scored as positive when ≥ 25% and ≥ 1% of cancer cells were stained positive, respectively. The human mutL homolog 1 and human mutS homolog 2 expressions were assessed by immunohistochemistry in PDAs to investigate mismatch-repair (MMR) status.Tumors that did not express either of these two were considered MMR-deficient. Results were analyzed for associations with clinicopathologic variables and the prognosis in individual histological CRC subtypes. RESULTS: MUC2-positive and MUC5AC-positive WMDA percentages were 49.2% and 30.2%, respectively. In contrast, MUC2-positive and MUC5AC-positive PDA percentages were 9.5% and 51.6%, respectively. MUC2 levels tended to decrease and MUC5AC levels tended to increase from WMDA to PDA. In 21 tumors comprising both adenoma and adenocarcinoma components in a single tumor (4 WMDAs, 7 PDAs, and 10 MUAs), MUC2 was significantly downregulated in PDA and MUC5AC was downregulated in PDA and MUA in the adenoma-carcinoma sequence. These results suggested that MUC2 levels might be associated with malignant potential and that MUC5AC expression was an early event in tumorigenesis. Despite worse prognoses than WMDA, high MUC2 expression levels were maintained in MUA (95.1%) and SRCC (71.5%), which suggested a pathogenesis for these subtypes distinct from that of WMDA. No significant associations were found between MUC2 expression and any clinicopathologic variables in any histological subtype. MUC5AC expression in PDA was closely associated with right-sided location (P = 0.017), absence of nodal metastasis (P = 0.010), low tumor node metastasis stage (P = 0.010), and MMR deficiency (P = 0.003). MUC2 expression in WMDA was a marginal prognostic factor for recurrence/metastasis-free survival (RFS) by univariate Cox analysis (P = 0.077) but not by multivariate Cox analysis (P = 0.161). MUC5AC expression in PDA was a significant prognostic factor for RFS by univariate Cox analysis (P = 0.007) but not by multivariate Cox analysis (P = 0.104). Kaplan-Meier curves and log-rank tests revealed that MUC2 expression was marginally associated with a better WMDA prognosis [P = 0.064 for RFS and P = 0.172 for overall survival (OS)] but not for PDA. In contrast, MUC5AC expression was significantly and marginally associated with a better PDA prognosis in terms of RFS and OS, respectively(P = 0.004 for RFS and P = 0.100 for OS), but not for WMDA and MUA. CONCLUSION: Mucin core protein expression profiles and clinical significance differ according to histological CRC subtypes. This may reflect different pathogeneses for these tumors. | Yasuo Imai Hidetsugu Yamagishi Kazunori Fukuda Yuko Ono Tohru Inoue Yoshihiko Ueda | 2013 | World Journal of Gastroenterology2013，19，25： | 14 |
| 3 | Herbal cardiotonic pills prevent gut ischemia/reperfusion-induced hepatic microvascular dysfunction in rats fed ethanol chronically显示文摘AIM: Cardiotonic Pill (CP), an oral herbal medicine that includes Danshen (Salviae Miltiorrhizae), Panax notoginseny and Dyroblanops aromatica gaertn, has been clinically used for vascular diseases such as occlusive vasculitis, coronary diseases, atherosclerosis, and cerebral infarction. The main component, SaMae Miltiorrhizae, has been reported to prevent cerebral and intestinal reperfusion injury. However, little is known about the effect of CP on hepatic microcirculation.Thus, this study aimed to determine whether CP could affect hepatic microvascular dysfunction elicited by gut ischemia/reperfusion (I/R) in rats fed ethanol chronically.METHODS: Male Wistar rats were pair-fed with a liquid diet containing ethanol or isocaloric control diet for 6 wk. After laparotomy, one lobe of the liver was examined through an inverted intravital microscope. The rats were exposed to 30 rain of gut ischemia followed by 60 rain of reperfusion.Rhodamine-6G-labeled leukocytes in the sinusoids were observed 90 rain after the onset of superior mesenteric artery ocdusion. Plasma tumor necrosis factor (TNF)-α and endotoxin levels were measured 1 h after the onset of reperfusion.Plasma alanine aminotransferase (ALT) activities were measured 6 h after the onset of reperfusion. In another set of experiments, CP (0.8 g/kg, intragastrically) was administered 1 and 24 h before the onset of ischemia.RESULTS: In control rats, gut I/R elicited increases in the number of stationary leukocytes, and plasma TNF-α and endotoxin levels and plasma ALT activities. These changes were mitigated by pretreatment with CP. In ethanol-fed rats,the gut I/R-induced increases in the number of stationary leukooltes, plasma endotoxin levels and ALT activities were enhanced. Pretreat^nent with CP attenuated the enhancement of gut I/R-induced responses by chronic ethanol consumption.CONCLUSION: These results suggest that CP prevents the gut I/R-induced hepatic microvascular dysfunction and hepatocellular injury. A reduction of inflammatory responses such as TNF-α production via reduction of blood endotoxin levels appears to be involved in the mechanisms. Chronic ethanol consumption enhances gut I/R-induced hepatic microvascular and hepatocellular injury. CP also attenuates an enhancement of gut I/R-induced responses by chronic ethanol consumption via the reduction of blood endotoxin levels. | Yoshinori Horie Shuka Mori Masahiro Konishi Mikio Kajihara Takehiko Kaneko Yoshiyuki Yamagishi Shinzo Kato Hiromasa Ishii Toshifumi Hibi | 2005 | World Journal of Gastroenterology2005，11，4： | 13 |
| 4 | A review of target gene specificity of flavonoid R2R3-MYB transcription factors and a discussion of factors contributing to the target gene selectivity显示文摘Flavonoid biosynthetic 基因并列地经常在花或水果开发期间以一种时间的方式被调整,导致 flavonoid 混合物的特定的累积侧面。R2R3-MYB-type 抄写因素(TF ) 成员生产 flavonoids 的一套 biosynthetic 基因,并且,因此, R2R3-MYBs 为结构的基因的协调表示负责。尽管很多信息关于鉴别那涉及 flavonoid 累积并且机能上地描绘了 R2R3-MYBs 迄今为止是可得到的,这是在 flavonoid 小径的 MYB 因素的全球规定上的第一评论。在这评论介绍的数据证明 anthocyanin, flavone/flavonol/3-deoxyflavonoid (FFD ) , proanthocyanidin (PA ) ,和 isoflavonoid 被 R2R3-MYBs 的不同亚群独立地调整。而且, FFD 特定的 R2R3-MYBs 作为他们的目标基因为早 biosynthetic 基因(EBG ) 有偏爱;从 dicot 种类的 anthocyanin 特定的 R2R3-MYBs 实质上调整迟了的 biosynthetic 基因(LBG ) ;留下的 R2R3-MYBs 有大量目标基因特性。阐明在 R2R3-MYBs 之间的微分目标基因特性的性质,我们分析了 DNA 有约束力的领域(也称为 MYB 域) R2R3-MYBs 和识别 cis 元素的分发。我们识别了位于或就在可能说明不同识别 DNA 顺序并且随后的 dicot anthocyanin R2R3-MYBs 的 helix-3 前的四保存氨基酸残余不同目标基因特性到留下的 R2R3-MYB TF。 | Yunsong LAI Huanxiu LI Masumi YAMAGISHI | 2013 | Frontiers in Biology2013，8，6： | 13 |
| 5 | Surgical treatment of HCC in a patient with lamivudine-resistant hepatitis B cirrhosis with adefovir dipivoxil显示文摘We describe a 77-year-old woman with chronic hepatitis B who became resistant to lamivudine.She was started on adefovir(10 mg daily)while still continuing lamivudine therapy.Four mo later her liver function improved and serum Hepatitis B virus(HBV)-DNA level became undetectable.Three years after the start of additional adefovir treatment,hepatocellular carcinoma (HCC)was detected and the patient underwent a successful hepa-tectomy.Our findings suggest tha-t the addition of adefovir to ongoing lamivudine therapy cannot completely suppress hepatocarcinogenesis,but is useful for improving liver function in patients with lamivudine-resistant HBV-related cirrhosis,allowing HCC surgery. | Takashi Akima Masaya Tamano Hidetsugu Yamagishi Keiichi Kubota Takahiro Fujimori Hideyuki Hiraishi | 2010 | World Journal of Hepatology2010，2，8： | 11 |
| 6 | Feasibility of single-incision laparoscopic cholecystectomy for acute cholecystitis显示文摘AIM: To assess the safety of single-incision laparoscopic cholecystectomy(SILC) for acute cholecystitis.METHODS: All patients who underwent SILC at Sano Hospital(Kobe, Japan) between January 2010 and December 2014 were included in this retrospective study. Clinical data related to patient characteristics and surgical outcomes were collected from medical records. The parameters for assessing the safety of the procedure included operative time, volume of blood loss, achievement of the critical view of safety, use of additional trocars, conversion to laparotomy, intraoperative and postoperative complications, and duration of postoperative hospital stay. Patient backgrounds were statistically compared between those with and without conversion to laparotomy.RESULTS: A total of 100 patients underwent SILC for acute cholecystitis during the period. Preoperative endoscopic treatment was performed for suspected choledocholithiasis in 41 patients(41%). The mean time from onset of acute cholecystitis was 7.7 d. According to the Updated Tokyo Guidelines(TG13) for the severity of cholecystitis, 86 and 14 patients had grade Ⅰ and grade Ⅱ acute cholecystitis, respectively. The mean operative time was 87.4 min. The mean estimated blood loss was 80.6 mL. The critical view of safety was obtained in 89 patients(89%). Conversion laparotomy was performed in 12 patients(12%). Postoperative complications of Clavien-Dindo grade Ⅲ or greater were observed in 4 patients(4%). The mean duration of postoperative hospital stay was 5.7 d. Patients converted from SILC to laparotomy tended to have higher days after onset.CONCLUSION: SILC is feasible for acute cholecystitis; in addition, early surgical intervention may reduce the risk of laparotomy conversion. | Taro Ikumoto Hidetsugu Yamagishi Mineo Iwatate Yasushi Sano Masahito Kotaka Yasuo Imai | 2015 | World Journal of Gastrointestinal Endoscopy2015，7，19： | 8 |
| 7 | Pseudolymphoma of the liver associated with primary biliary cirrhosis:A case report and review of literature显示文摘We report a case of two pseudolymphomas of the liver in a 63-year-old Japanese woman with primary biliary cirrhosis.One of the lesions was found incidentally during a medical examination,presenting as a 10 mm hypodense nodule that revealed hyperdensity in the early phase and hypodensity in the late phase in computed tomography(CT)after injection of contrast medium.Retrospectively,the 10 mm nodule had first been discovered as a 4 mm nodule during CT 4 years previously.Superparamagnetic iron oxide-enhanced MRI revealed another 4 mm hyperintense nodule in segment 6 in addition to the 10 mm hyperintense nodule in segment 7.CT during arterial portography revealed two hypointense nodules.Findings with other imaging modalities such as ultrasonography,magnetic resonance imaging,and hepatic angiography were consistent with hepatocellular carcinoma.A right posterior segmentectomy was performed,and the lesions were microscopically diagnosed as pseudolymphoma.To the best of our knowledge,only 31 other cases of this disease have ever been reported,with a highly asymmetrical male:female ratio of 1:9.7.Although we could find only one case of transformation of hepaticpseudolymphoma into lymphoma in the liver,the exact nature of development from benign pseudolymphoma to malignant lymphoma is still not fully understood and cases of hepatic lymphoma need to be followed carefully. | Toshihide Okada Hiroshi Mibayashi Kenkei Hasatani Yoshiaki Hayashi Shigetsugu Tsuji Yoshibumi Kaneko Masashi Yoshimitsu Takashi Tani Yoh Zen Masakazu Yamagishi | 2009 | World Journal of Gastroenterology2009，15，36： | 8 |
| 8 | Effects of Weak Light on Starch Accumulation and Starch Synthesis Enzyme Activities in Rice at the Grain Filling Stage显示文摘Dynamic changes of starch, amylose, sucrose contents and the activities of starch synthesis enzymes under shading treatments after flowering were studied using two rice varieties IR72 (indica) and Nipponbare (japonica) as materials. Under shading treatments, the starch, amylose and sucrose contents decreased, while ADP-glucose pyrophosphorylase (ADPGPPase) activity only changed a little, soluble starch synthase activity and granule bound starch synthase activity decreased, soluble starch branching enzyme (SSBE, Q-enzyme) activity and granule bound starch branching enzyme (GBSBE, Q-enzyme) activity increased, and starch debranching enzyme (DBE, R-enzyme) activity varied with varieties. Correlation analyses showed that the changes of starch content were positively and significantly correlated with the changes of sucrose content in the weak light. Both ADPGPPase activity and SSBE activity werep ositively and significantly correlated with starch accumulation rate. It was implied that the decline of starch synthase activities was related to the decrease of starch content and the increase of the activity of starch branching enzyme played an important role in the decrease of the ratio of amylose to the total starch under the weak light. | LI Tian Ryu OHSUGI Tohru YAMAGISHI Haruto SASAKI | 2006 | Rice science2006，13，1： | 7 |
| 9 | 太阳耀斑和相关电离层吸收事件显示文摘利用23周太阳峰期期间南极中山站(不变磁纬74.5°S)成像式宇宙噪声接收机的观测结果, 对由太阳质子和X射线耀斑引起的日侧电离层吸收事件进行了分析, 定量地给出电离层吸收和X射线耀斑强度的对应关系, 同时还利用北极Ny-Alesund(不变磁纬76.08癗)站的观测数据对此关系进行对比研究, 得出了在理论和观测上都较为一致的结论. 同时, 认为M级以上的X射线耀斑才能引起日侧电离层较为明显的吸收. | 贺龙松 M. Nishino 张北辰 刘勇华 刘瑞源 刘顺林 胡红桥 N.Sato H. Yamagishi | 2000 | 科学通报2000，45，17： | 7 |
| 10 | Molecular pathogenesis of sporadiccolorectal cancers显示文摘Colorectal cancer(CRC)results from the progressive accumulation of genetic and epigenetic alterations that lead to the transformation of normal colonic mucosa to adenocarcinoma.Approximately 75%of CRCs are sporadic and occur in people without genetic predisposition or family history of CRC.During the past two decades,sporadic CRCs were classified into three major groups according to frequently altered/mutated genes.These genes have been identified by linkage analyses of cancer-prone families and by individual mutation analyses of candidate genes selected on the basis of functional data.In the first half of this review,we describe the genetic pathways of sporadic CRCs and their clinicopathologic features.Recently,large-scale genome analyses have detected many infrequently mutated genes as well as a small number of frequently mutated genes.These infrequently mutated genes are likely described in a limited number of pathways.Gene-oriented models of CRC progression are being replaced by pathway-oriented models.In the second half of this review,we summarize the present knowledge of this research field and discuss its prospects. | Hidetsugu Yamagishi Hajime Kuroda Yasuo Imai Hideyuki Hiraishi | 2016 | Chinese Journal of Cancer2016，35，1： | 7 |
| 11 | Treatment of bleaching wastewater from pulp paper plants in China using enzymes and coagulants显示文摘The treatment of wastewater from pulp\|paper plants in China by horseradish peroxidase was investigated in this study. The effects of horseradish peroxidase and coagulants were discussed in detail. The results indicated that enzymes might improve the removal of AOX, TOC and colour for pulp\|paper wastewater and modified chitosan is far more effective than Al\-2(SO\-4)\-3 to remove AOX, TOC and colour. | ZHANG Tong\+1, Shinji Wada\+2, Takao Yamagishi\+2, Ichikawa Hiroyasu\+2, Kenji Tatsumi\+ 2* , ZHAO Qing\|xiang\+1 (1. Department of Environmental Engineering, East China University of Science and Technology, Shanghai 200237,China 2. National Institut | 1999 | Journal of Environmental Sciences1999，11，4： | 5 |
| 12 | Effect of High Temperature on Sucrose Content and Sucrose Cleaving Enzyme Activity in Rice Grain During the Filling Stage显示文摘Dynamic changes of sucrose, fructose, glucose contents and differences in activities of sucrose synthase, vacuolar invertase, and cell wall bound invertase in rice grain after flowering stage were studied under natural and high temperatures by using two japonica rice varieties Koshihikari and Sasanishiki. In rice grains, the sucrose synthase activity was higher than that of invertase, which was significantly correlated with starch accumulation rate, indicating that the sucrose synthase played an important role in sucrose degradation and starch synthesis. Under high temperature, the significant increase in grain sucrose content without any increase in fructose and glucose contents, suggested that the high temperature treatment enhanced sucrose accumulation, while diminished sucrose degradation in rice grains. Compared with the control plants, the decrease in activities of sucrose synthase, vacuolar invertase, and cell wall bound invertase with high temperature treated plants indicated that the deceleration of sucrose-degradation was related to the decrease in activities of sucrose synthase and invertase. | LI Tian LIU Qi-hua Ryu OHSUGI Tohru YAMAGISHI Haruto SASAKI | 2006 | Rice science2006，13，3： | 5 |
| 13 | Long-term survival of a case with multiple liver metastases from duodenal gastrointestinal stromal tumor drastically reduced by the treatment with imatinib and hepatectomy显示文摘工具包受体酷氨酸激酶的组成的激活是在胃肠的基质肿瘤(大意) 的致病的一个关键因素。但是几乎没有 imatinib mesylate (IM ) 和外科疗法的联合是否能与 unresectable 在情况中延长幸存的很少信息多重肝转移。我们报导酷氨酸激酶禁止者 IM 和外科疗法对待的大意的手术后的复发的一个案例。对处理的起始的完全的反应(CR ) 为 18 瞬间继续,但是单个肝转移在 IM 处理期间在左肝的脑叶显示出生长。在再发性瘤的部分切除术以后,手术后的路线是平静的,没有复发,病人为 24 瞬间幸存。当前, imatinib 是为 non-resectable 大意的首要的治疗,但是单个代理人治疗经常导致肿瘤抵抗。就算到 imatinib 的忍耐发生, imatinib 和外科疗法的联合能在这里报导了的一些情况中延长幸存。然而,周期性的大意的很多案例上的进一步的研究是必要的评估治疗与外科相结合的 IM 的有效性。 | Chouhei Sakakura Akeo Hagiwara Koji Soga Koji Miyagawa Susumu Nakashima Tetsuji Yoshikawa Shuichi Kin Yuenn Nakase Nobuki Yamaoka Yoshihiko Sagara Hisakazu Yamagishi | 2006 | World Journal of Gastroenterology2006，12，17： | 5 |
| 14 | Decreased hemoglobin after initial treatment is associated with treatment resistance in Kawasaki disease in Kobayashi risk stratification显示文摘Background Although Kawasaki disease(KD)is known to cause anemia,the relationship between its prognosis and hemoglobin level is less well known.Methods We herein performed a secondary analysis of data from a previous study(Post-RAISE).Children aged 6 months to 2 years at the time of KD diagnosis were enrolled.Decreased hemoglobin was defined as a decrease>10%on post-treatment day 2 compared with the level prior to the initial therapy.Results Of 1253 KD cases,treatment resistance was observed in 209(17%)and was significantly more frequent in the decreased hemoglobin group(37/167 vs.172/1086,P=0.041).Multivariable logistic regression analysis revealed that decreased hemoglobin was associated with resistance to the initial treatment(odds ratio 1.53;95%confidence interval 1.00-2.33;P=0.048).Conclusions Decreased hemoglobin was significantly associated with resistance to initial treatment in patients with KD. | Yukari Atsumi Hiroshi Sakakibara Yoshihiko Morikawa Koichi Miyata Hiroyuki Yamagishi Masahiro Misawa Masaru Miura | 2020 | World Journal of Pediatrics2020，16，6： | 5 |
| 15 | Involvement of the TAGE-RAGE system in non-alcoholic steatohepatitis: Novel treatment strategies显示文摘Non-alcoholic fatty liver disease(NAFLD)is a major cause of liver disease around the world.It includes a spectrum of conditions from simple steatosis to non-alcoholic steatohepatitis(NASH)and can lead to fibrosis,cirrhosis,liver failure,and/or hepatocellular carcinoma.NAFLD is also associated with other medical conditions such as obesity,diabetes mellitus(DM),metabolic syn-drome,hypertension,insulin resistance,hyperlipidemia,and cardiovascular disease(CVD).In diabetes,chronic hyperglycemia contributes to the development of both macro-and microvascular conditions through a variety of metabolic pathways.Thus,it can cause a variety of metabolic and hemodynamic conditions,including upregulated advanced glycation end-products(AGEs)synthesis.In our previous study,the most abundant type of toxic AGEs(TAGE);i.e.,glyceraldehyde-derived AGEs,were found to make a significant contribution to the pathogenesis of DM-induced angiopathy.Furthermore,accumulating evidence suggests that the binding of TAGE with their receptor(RAGE)induces oxidative damage,promotes inflammation,and causes changes in intracellular signaling and the expression levels of certain genes in various cell populations including hepatocytes and hepatic stellate cells.All of these effects could facilitate the pathogenesis of hypertension,cancer,diabetic vascular complications,CVD,dementia,and NASH.Thus,inhibiting TAGE synthesis,preventing TAGE from binding to RAGE,and downregulating RAGE expression and/or the expression of associated effector molecules all have potential as therapeutic strategies against NASH.Here,we examine the contributions of RAGE and TAGE to various conditions and novel treatments that target them in order to prevent the development and/or progression of NASH. | Masayoshi Takeuchi Jun-ichi Takino Akiko Sakasai-Sakai Takanobu Takata Tadashi Ueda Mikihiro Tsutsumi Hideyuki Hyogo Sho-ichi Yamagishi | 2014 | World Journal of Hepatology2014，6，12： | 5 |
| 16 | Recurrent chromosomal rearrangements at bands 8q24 and 11q13 in gastric cancer as detected by multicolor spectral karyotyping显示文摘AIM: To identify chromosomal translocations specific to gastric cancer (GC), spectral karyotyping (SKY) analysis was performed on established cell lines and cancerous ascitic fluids.METHODS: SKY analysis of 10 established cell lines and seven cancerous ascitic fluid samples identified recurrent chromosomal breakpoints and translocations in GC,several of which involved chromosomal loci of oncogenes or tumor suppressor genes.RESULTS: A total of 630 chromosomal breaks were identified. Chromosome no.8 was the most frequently involved in rearrangements (65 breaks), followed by chromosomes no. 11 (53), no. 1 (49), no. 7 (46), no. 13 (37), no. 3 (36), no. 17 (33), and no. 20 (29). Frequent breakpoints were detected in 8q24.1 (30 breaks), 11q13 (29), 13q14 (16), 20q11.2 (14), 7q32 (13), 17q11.2 (13),18q21 (12), 17q23 (9), 18q11.2 (9). SKY analysis identified a total of 242 chromosomal rearrangements including 190 reciprocal and non-reciprocal translocations. The recurrent combinations of chromosomal bands involved in translocations were 8q24.1 and 13q14 (3 cases), 8q24.1 and 11q13 (3), 11q13 and 17q11.2 (2), and 18q11.2 and 20q11.2 (2). Our study validated the ability of SKY to characterize in detail the chromosomal rearrangements in solid tumors and derived cell lines. Moreover,fluorescence in situ hybridization helped to identify the insertions, translocations, and homogeneously staining regions of MYCand CCND1 gene loci.CONCLUSION: The non-random co-localization of certain cytogenetic bands suggests the importance of chromosomal translocations in gastric carcinogenesis, by serving as landmarks for the cloning of GC causing genes. | Yasuhide Yamashita Kazuhiro Nishida Takashi Okuda Kenichi Nomura Yosuke Matsumoto Shoji Mitsufuji Shigeo Horiike Hiroyuki Hata Chohei Sakakura Akio Hagiwara Hisakazu Yamagishi Masafumi Taniwaki | 2005 | World Journal of Gastroenterology2005，11，33： | 4 |
| 17 | Toosendanin-induced Ca^(2+) Conductance Increase in Neuroblastoma×Giioma Hybrid Cells显示文摘Toosendanin (TSN), a triterpenoid derivative extracted from the bark of Melia toosendan Seib et Zucc and used in Chinese traditional medicine as an anthelmintic against ascaris, is a presynaptic neuromuscular blocker. Without affecting the nerve conduction, the muscle resting potential as well as acetylcholine (ACh) sensitivity of the muscle membrane at the end-plate region, TSN blocks the neuromuscular transmission selectively by inhibiting ACh release from the motor nerve terminals. It was interesting to notice | 施玉樑 Furuya Kishio 王文萍 Terakawa Susumu 徐科 Yamagishi Shunichi | 1993 | Chinese Science Bulletin1993，38，17： | 4 |
| 18 | Atorvastatin decreases serum levels of advanced glycation endproducts (AGEs) in nonalcoholic steatohepatitis (NASH) patients with dyslipidemia: clinical usefulness of AGEs as a biomarker for the attenuation of NASH显示文摘 | Yuki Kimura Hideyuki Hyogo Sho-ichi Yamagishi Masayoshi Takeuchi Tomokazu Ishitobi Yoshitaka Nabeshima Koji Arihiro Kazuaki Chayama | 2010 | Journal of Gastroenterology2010，，7： | 3 |
| 19 | Can magnifying endoscopy with narrow band imaging be useful for low grade adenomas in preoperative biopsy specimens?显示文摘 | Kazuhiro Miwa Hisashi Doyama Renma Ito Hiroyoshi Nakanishi Katsura Hirano Satoko Inagaki Kei Tominaga Naohiro Yoshida Kenichi Takemura Shinya Yamada Yoshibumi Kaneko Kazuyoshi Katayanagi Hiroshi Kurumaya Toshihide Okada Masakazu Yamagishi | 2012 | Gastric Cancer2012，，2： | 3 |
| 20 | Clinical importance of colonoscopy in patients with gastric neoplasm undergoing endoscopic submucosal dissection显示文摘AIM To evaluate the usefulness of total colonoscopy(TCS) for patients undergoing gastric endoscopic submucosal dissection(ESD) and to assess risk factors for colorectal neoplasms.METHODS Of the 263 patients who underwent ESD at our department between May 2010 and December 2013, 172 patients undergoing TCS during a one-year period before and after ESD were targeted. After excluding patients with a history of surgery or endoscopic therapy for colorectal neoplasms, 158 patients were analyzed. Of the 868 asymptomatic patients who underwent TCS during the same period because of positive fecal immunochemical test(FIT) results, 158 patients with no history of either surgery or endoscopic therapy for colorectal neoplasms who were matched for age and sex served as the control group for comparison.RESULTS TCS revealed adenoma less than 10 mm in 53 patients(33.6%), advanced adenoma in 17(10.8%), early colorectal cancer in 5(3.2%), and advanced colorectalcancer in 4(2.5%). When the presence or absence of adenoma less than 10 mm, advanced adenoma, and colorectal cancer and the number of adenomas were compared between patients undergoing ESD and FITpositive patients, there were no statistically significant differences in any of the parameters assessed. The patients undergoing ESD appeared to have the same risk of colorectal neoplasms as the FIT-positive patients. Colorectal neoplasms were clearly more common in men than in women(P = 0.031). Advanced adenoma and cancer were significantly more frequent in patients with at least two of the following conditions: hypertension, dyslipidemia, and diabetes mellitus(P = 0.019).CONCLUSION In patients undergoing gastric ESD, TCS appears to be important for detecting synchronous double neoplasms. Advanced adenoma and cancer were more common in patients with at least two of the following conditions: hypertension, dyslipidemia, and diabetes mellitus. Caution is therefore especially warranted in patients with these risk factors. | Chieko Tsuchida Naoto Yoshitake Hitoshi Kino Yoshihito Kaneko Masakazu Nakano Kohei Tsuchida Keiichi Tominaga Takako Sasai Hironori Masuyama Hidetsugu Yamagishi Yasuo Imai Hideyuki Hiraishi | 2017 | World Journal of Gastroenterology2017，23，23： | 3 |
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