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1A reappraisal of CTLA-4 checkpoint blockade in cancer immunotherapy显示文摘anti-CTLA-4 抗体由阻止 B7-CTLA-4 相互作用接近否定发信号引起肿瘤拒绝,这被假定。在比临床上有效的 dosing 完成的血浆层次更加高的集中,令人惊讶地, anti-CTLA-4 抗体 Ipilimumab 不由 CTLA-4 也不 CTLA-4 绑定堵住任何一 B7 trans-endocytosis 到使不能调动或联系房间的 B7。因而, Ipilimumab 不从人性化的任何一个 CTLA4 基因在树枝状的房间(DC ) 上增加 B7 (Ctla4 h/h ) 或人的 CD34 + 茎重新组成房间的 NSG 老鼠。在 Ctla4 高效地表示人和老鼠 CTLA4 基因,绑在人的 anti-CTLA-4 抗体然而并非老鼠 CTLA-4 的 h/m 老鼠导致 Treg 弄空和 Fc 受体依赖者肿瘤拒绝。堵住的抗体 L3D10 比得上在引起肿瘤拒绝的非阻塞的 Ipilimumab。显著地,输在人类化期间堵住活动的 L3D10 子孙在导致 Treg 弄空和肿瘤拒绝仍然保持充分能干。有效地在淋巴的器官堵住 CD4 T 细胞激活和 de novo CD8 T 细胞 priming 的 Anti-B7 抗体否定地不影响 Ipilimumab 的 immunotherapeutic 效果。因此,临床上有效的 anti-CTLA-4 mAb 由独立于检查点封锁的机制引起肿瘤拒绝但是依赖于主人 Fc 受体。我们为 CTLA-4 检查点的一个重评价的数据电话封锁假设并且为安全、有效的 anti-CTLA-4 mAbs 的下一代提供新卓见。Xuexiang Du Fei Tang Mingyue Liu Juanjuan Su Yan Zhang Wei Wu Martin Devenport Christopher A Lazarski Peng Zhang Xu Wang Peiying Ye Changyu Wang Eugene Hwang Tinghui Zhu Ting Xu Pan Zheng Yang Liu 2018Cell Research2018,28,4:25
2A Comparison of Carlin-type Gold Deposits:Guizhou Province,Golden Triangle,Southwest China,and Northern Nevada,USA显示文摘Several Au deposits in Guizhou Province,southwest China,described as being similar to the highly productive Carlin-type gold deposits in northern Nevada.USA,were examined to identify similarities and differences between the two districts.Samples were collected along transects from lowto high-grade rock,where possible,and fram stockpiles at the Shuiyindong,Zimudang,Taipingdong,Yata and Jinfeng(formerly Lannigou)deposits.Methods used to examine ore and alteration minerals included hand-sample description:reflectance spectroscopy using an ASD Terraspec spectrometer;analyses of hand samples by carbonate staining with Alizaren red and potassium ferricyanide;transmitted and reflected light petrography;chemical analyses,mineral identification,and imaging using a JEOL.JSM-5610 scanning electron microscope:and quantitative chemical analyses using a JEOL JXA-8900 electron probe microanalyzer.Geochemical analyses of hand samples for 52 elements were done by ALS Chemex.Results indicate both similarities and differences between the two districts.Both districts have similar geologic histories.and deposits at both locations appear to have formed as a result of similar tectonic events.though the district in southwest China lacks evidence of eoeval felsic igneous activity;however,the ore-stage minerals and the fluids that produced the minerals and deposits have some significant differences.The Nevada deposits were dominated by fluid-rock reaction in which host rock Fe was sulfidized to form Au-bearing pyrite.Although ore fluids sulfidized host rock Fe in the Cuizhou deposits.the timing of Fe metasomatism is unknown,so whether the deposits formed in response to sulfidation or pyritization is unclear.Fluid-rock reaction between an acidic,aqueous fluid and highly reactive calcareous rocks in Nevada caused extensive decarbonatization of host rocks,jasperoid replacemerit of carbonate minerals,and alteration of silty rock components to illite and kaolinite.In Guizhou,CO2-bearing ore fluids with temperatures and pressures approaching 100℃ and 500 bars greater than temperatures and pressures detemained for ore fluids in the Nevada deposits,deposited Aubearing pyrite.In examined Guizhou deposits these fluids carbonatized host rocks and farmed both replacement and open-space-filling ore.The fluids,which may have been immiscible,were sufficiently overpressured to fracture wall rocks and to create significant open space filled by vein quartz.While deposit architecture,tectonic setting,and host rocks in Guizhou are quite similar to northern Nevada,ore and alteration minerals suggest that ore-forming processes in examined Guizhou deposits have important similarities to processes associated with formation of orogenic Au systems.The Guizhou deposits display characteristics of both Carlin-type and orogenic systems,perhaps indicating formation at conditions somewhat intermediate to conditions for Carlin-type deposits and orogenic systems.Jean S.CLlNE John L.MUNTEAN Xuexiang GU Yong XIA 2013地学前缘2013,20,1:23
3Lycorine inhibits breast cancer growth and metastasis via inducing apoptosis and blocking Src/FAK-involved pathway显示文摘Breast cancer is the most commonly diagnosed cancer type worldwide among women and more than 90% of patients die from tumor metastasis. Lycorine, a natural alkaloid, has been widely reported possessing potential efficacy against cancer proliferation and metastasis. In our study, the anti-tumor potency on breast cancer was evaluated in vitro and in vivo for the first time. Our results indicated that lycorine inhibited breast cancer cells growth, migration and invasion as well as induced their apoptosis.In in vivo study, lycorine not only suppressed breast tumor growth in xenograft models and inhibited breast tumor metastasis in MDA-MB-231 tail vein model. More importantly, we found lycorine had less toxicity than first-line chemotherapy drug paclitaxel at the same effective dose in vivo. Furthermore, on mechanism, lycorine inhibited tumor cell migration and invasion via blocking the Src/FAK(focal adhesion kinase)-involved pathway. In conclusion, our study implied lycorine was a potential candidate for the treatment of breast cancer by inhibition of tumor growth and metastasis.Xuexiang Ying Anling Huang Yajing Xing Liping Lan Zhengfang Yi Pingqing He 2017Science China(Life Sciences)2017,60,4:15
4CD59 Silencing via Retrovirus-Mediated RNA Interference Enhanced Complement-Mediated Cell Damage in Ovary Cancer显示文摘CD59,belonging to membrane complement regulatory proteins(mCRPs) ,inhibits the cytolytic activity of complement and is over-expressed in solid cancers,including ovary cancer. The aim of the present study was to construct recombinant retrovirus encoding shRNA targeted human CD59 and infect A2780 cells in order to investigate the relationship between decreased CD59 expression and tumorigenesis of ovary cancer. siCD59 and siCD59-C were successfully constructed and identified by PCR,restriction endonuclease analyses and DNA sequencing,respectively. The siCD59 was able to efficiently infect A2780 cells,which was confirmed by Western blotting. When incubated with fresh normal human serum(8%,v/v) for 1 h at 37°C,the cell viability was decreased and cell damage was increased in siCD59 infected A2780 cells compared to siCD59-C infected cells. This led to the activation of caspase-3. The apoptosis in siCD59 infected cells was shown with hypercondensed nuclei using Hoechst staining. Meanwhile,the weight of ovary tumor graft in nude mice was significantly decreased in siCD59 group compared to that of siCD59-C group. And the expression of CD59 protein in tumor tissue in siCD59 group was significantly decreased. These results suggested that CD59 silencing in ovary cancer cells via retrovirusmediated RNAi can enhance complement-mediated cell damage,inhibiting growth of ovary cancer. CD59 might be a potential target for gene therapy in ovary cancer.Xuexiang Shi Bei Zhang Jinlin Zang Guoying Wang Meihua Gao 2009Cellular & Molecular Immunology2009,6,1:12
5Quantitative assessment of acute kidney injury by noninvasive arterial spin labeling perfusion MRI:a pilot study显示文摘The kidneys are essential for maintaining homeostasis,are responsible for the reabsorption of water,glucose and amino acids,and filter the blood by removing waste.Acute kidney injury(AKI) is a syndrome characterized by the rapid loss of renal excretory function and the accumulation of end metabolic products of urea and creatinine.AKI is associated with the later development of chronic kidney disease and end-stage kidney disease,and may eventually be fatal.Early diagnosis of AKI and assessments of the effects of treatment,however,are challenging.The pathophysiological mechanism of AKI is thought to be the imbalance between oxygen supply and demand in the kidneys.We have assessed the ability of arterial spin labeling(ASL) perfusion magnetic resonance imaging(MRI),without the administration of contrast media,to quantify renal blood flow(RBF) non-invasively.We found that RBF was significantly lower in AKI patients than in healthy volunteers.These results suggest that ASL perfusion MRI,a noninvasive measurement of RBF,may be useful in the early diagnosis of AKI.DONG Jian YANG Li SU Tao YANG XueDong CHEN Bin ZHANG Jue WANG XiaoYing JIANG XueXiang 2013Science China(Life Sciences)2013,56,8:8
6Sequential use of ultraviolet light and chlorine for reclaimed water disinfection显示文摘Several disinfection processes of ultraviolet (UV),chlorine or UV followed by chlorine were investigated in municipal wastewater according to the inactivation of Escherichia coli,Shigella dysenteriae and toxicity formation.The UV inactivation of the tested pathogenic bacteria was not affected by the quality of water.It was found that the inactivated bacteria were obviously reactivated after one day in dark.Fluorescent light irradiation increased the bacteria repair.The increase of UV dosage could cause more damage to bacteria to inhibit bacteria self-repair.No photoreactivation was detected when the UV dose was up to 80 mJ/cm2 for E.coli DH5α,and 23 mJ/cm2 for S.dysenteriae.Nevertheless,sequential use of 8 mJ/cm2 of UV and low concentration of chlorine (1.5 mg/L) could effectively inhibit the photoreactivation and inactivate E.coli below the detection limits within seven days.Compared to chlorination alone,the sequential disinfection decreased the genotoxicity of treated wastewater,especially for the sample with high NH 3-N concentration.Xiujuan Wang Xuexiang Hu Chun Hu Dongbin Wei 2011Journal of Environmental Sciences2011,23,10:8
7The Effect of Tide on the Global Climate Change显示文摘The differential rotation between the solid and fluid spheres caused by tidal force could explain the 1500 to 1800-year cycle of the world's temperature. Strong tide increases the vertical and horizontal mixing of water in the oceans, drawing the cold Pacific water from the depths to the surface and the warm water from the west to the east, where it cools or warms the atmosphere above, absorbs or releases CO2 to decrease or increase greenhouse effect and to make La Nina or El Nino occur in the global. The moon's declination and obliquity of the ecliptic affect the tidal intensity. The exchange of tidal energy and tide-generating force caused by the sun, moon and major planets makes the earth's layers rotate in different speeds. The differenti-al rotation between solid and fluid of the earth is the basic reason for El Nino and global climate change.YANG Xuexiang, CHEN Zhen and CHEN Dianyou( College of Ceo - exploration Science and Technology, Jilin University, Changchun 130026, P. R. China)Qiao Qiyuan(National Astronomical Observatory, Chinese Academy of Science, Beijing 100012, P. R. China) 2002Global Geology2002,7,1:7
8Uncoupling therapeutic from effects for safer and effective CTLA4 humanized mice immunotherapy-related adverse anti-CTLA-4 antibodies in显示文摘Xuexiang Du Mingyue Liu Juanjuan Su Peng Zhang Fei Tang Peiying Ye Martin Devenport Xu Wang Yan Zhang Yang Liu Pan Zheng 2018Cell Research2018,28,4:6
9Hijacking antibody-induced CTLA-4 lysosomal degradation for safer and more effective cancer immunotherapy显示文摘It remains unclear why the clinically used anti-CTLA-4 antibodies, popularly called checkpoint inhibitors, have severe immunotherapy-related adverse effects (irAEs) and yet suboptimal cancer immunotherapeutic effects (CITE). Here we report that while irAE-prone Ipilimumab and TremeIgG1 rapidly direct cell surface CTLA-4 for lysosomal degradation, the non-irAE-prone antibodies we generated, HL12 or HL32, dissociate from CTLA-4 after endocytosis and allow CTLA-4 recycling to cell surface by the LRBA-dependent mechanism. Disrupting CTLA-4 recycling results in robust CTLA-4 downregulation by all anti-CTLA-4 antibodies and confers toxicity to a non-irAE-prone anti-CTLA-4 mAb. Conversely, increasing the pH sensitivity of TremeIgG1 by introducing designed tyrosine-to-histidine mutations prevents antibody-triggered lysosomal CTLA-4 downregulation and dramatically attenuates irAE. Surprisingly, by avoiding CTLA-4 downregulation and due to their increased bioavailability, pH-sensitive anti-CTLA-4 antibodies are more effective in intratumor regulatory T-cell depletion and rejection of large established tumors. Our data establish a new paradigm for cancer research that allows for abrogating irAE while increasing CITE of anti-CTLA-4 antibodies.Yan Zhang Xuexiang Du Mingyue Liu Fei Tang Peng Zhang Chunxia Ai James K. Fields Eric J. Sundberg Olga S. Latinovic Martin Devenport Pan Zheng Yang Liu 2019Cell Research2019,29,8:6
10Radiomic analysis of pulmonary ground-glass opacity nodules for distinction of preinvasive lesions, invasive pulmonary adenocarcinoma and minimally invasive adenocarcinoma based on quantitative texture analysis of CT显示文摘Objective: To identify the differences among preinvasive lesions, minimally invasive adenocarcinomas(MIAs)and invasive pulmonary adenocarcinomas(IPAs) based on radiomic feature analysis with computed tomography(CT).Methods: A total of 109 patients with ground-glass opacity lesions(GGOs) in the lungs determined by CT examinations were enrolled, all of whom had received a pathologic diagnosis. After the manual delineation and segmentation of the GGOs as regions of interest(ROIs), the patients were subdivided into three groups based on pathologic analyses: the preinvasive lesions(including atypical adenomatous hyperplasia and adenocarcinoma in situ) subgroup, the MIA subgroup and the IPA subgroup. Next, we obtained the texture features of the GGOs. The data analysis was aimed at finding both the differences between each pair of the groups and predictors to distinguish any two pathologic subtypes using logistic regression. Finally, a receiver operating characteristic(ROC) curve was applied to accurately evaluate the performances of the regression models.Results: We found that the voxel count feature(P<0.001) could be used as a predictor for distinguishing IPAs from preinvasive lesions. However, the surface area feature(P=0.040) and the extruded surface area feature(P=0.013) could be predictors of IPAs compared with MIAs. In addition, the correlation feature(P=0.046) could distinguish preinvasive lesions from MIAs better.Conclusions: Preinvasive lesions, MIAs and IPAs can be discriminated based on texture features within CT images, although the three diseases could all appear as GGOs on CT images. The diagnoses of these three diseases are very important for clinical surgery.Wei Li Xuexiang Wang Yuwei Zhang Xubin Li Qian Li Zhaoxiang Ye 2018Chinese Journal of Cancer Research2018,30,4:6
11Initial movement of the Karakorum Fault in western Tibet:constraints from SHRIMP U-Pb dating of zircons显示文摘The Karakorum Fault zone(KFZ)plays an important role in understanding the formation,evolvement and deformation of the Tibetan Plateau.The high-T dextral shearing metamorphic rocks,e.g.,mylonites or mylonitized gneisses-granites,locally crop out along the southeastern part of the KFZ in the Ayila Ri’gyüRange area.The SHRIMP U-Pb dating of the syn-kinematic crystallized zircons indicates that the initial age of the KFZ is^27 Ma,~10 Ma older than previous results.The extensive high-T dextral shearing along the KFZ started at least at 27-20 Ma,accompanied by the syn-kinematic emplacement of leuco-granites.Deformation and concomitant fluid circulation during shearing most likely occurred as early as at 25-13 Ma.The KFZ probably grew from southeast to northwest along the fault as a result of continuous convergence between the India plate and Eurasia plate.LI HaiBing Franck VALLI LIU DunYi XU ZhiQin YANG JingSui Nicolas ARNAUD Paul TAPPONNIER Robin LACASSIN CHEN SongYong QI xuexiang 2007Chinese Science Bulletin2007,52,8:6
12The s-ordered expansions of the operator function about the combined quadrature μX + νP显示文摘A general framework applicable to deriving the s-ordered operator expansions is presented in this paper.We firstly deduce the s-ordered operator expansion formula of density operator ρ a?,a and introduce the technique of integration within the sordered product of operators (IWSOP).Based on the deduction and the technique,we derive the s-ordered expansions of operators (μX + νP)n and Hn (μX + νP) (linear combinations of the coordinate operator X and the momentum operator P,Hn (x) is Hermite polynomial),respectively,and discuss some special cases of s=1,0,-1.Some new useful operator identities are obtained as well.HongChun Yuan XueXiang Xu HongYi Fan 2010Science China(Physics,Mechanics & Astronomy)2010,53,10:4
13Selenium Enrichment in Cambrian Stratabound Gold Deposits in the Western Qinling Mountains显示文摘Stratabound gold deposits in the western Qinling Mountains occur in Cambrian chert formation composed of carbonaceous chert and carbonaceous slate. The distinctive chert formation provides important grounds for the mineralization and controls on the formation of gold deposits. Study shows that Se is exceptionally higher in both host rocks and gold orebodies. It may be recovered as a valuable component in ores for total utilization, and in some localities even independent Se orebodies (which are mined exclusively for Se) may be delineated. In gold ore Se mainly occurs as independent minerals or in the isomorphous form in sulphides and there is a positive correlation between Se and Au.Liu Jiajun,Open Laboratory of Ore Deposit Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, Guizhou 550002Zheng Minghua, Chengdu Institute of Technology, Chengdu, Sichuan 610059Liu Jianming, Research Centre of Mineral Resources Exploration, Chinese Academy of Sciences,Beijing 100101Zhou Yufeng, Gu Xuexiang, Zhang Bin, Lin Li, Chengdu Institute of Technology, Chengdu, Sichuan 610059and Lu Wenquan Institute of Comprehensive Utilization of Mineral Resources, Chengdu, Sichuan 610041 1997Acta Geologica Sinica(English Edition)1997,71,4:3
14Isolation,Purification and Immunomodifying Activity of Polysaccharides from Phellinus igniarius显示文摘Phellinus igniarius polysaccharides were obtained by hot water extraction of mushroom fruit bodies followed by ethanol precipitation,and further purified by anion exchange and gel filtration chromatography.Immuno-enhancing activity was evaluated by determining the effect of different polysaccharide fractions on mouse spleen lymphocyte proliferation in vitro.Three crude polysaccharide preparations,designated PIP30,PIP60 and PIP80 according to the percentage concentration of ethanol required for precipitation from hot aqueous extracts,promoted lymphocyte proliferation to varying degrees.Purification of polysaccharide present in PIP30 and PIP60 was carried out using anion exchange chromatography.Proliferation rates in samples treated with 200 μg/mL of fractions P30U,P30W and P30S1(obtained following anion exchange chromatography of PIP30) were approximately 5-,4-,and 3.5-fold higher,respectively compared with negative controls.Similarly,marked increases(3.4-and 2.8-fold) in cell proliferation rates compared with negative controls were observed with 200 μg/mL concentrations of P60W and P60S2(obtained following anion exchange chromatography of PIP60),respectively.Two purified polysaccharide preparations,P60W1-1 and P60S1-1,were obtained following gel filtation chromatography of fractions P60W1 and P0S1,respectively.Fraction P60W1-1(10-100 g/mL) enhanced splenocyte proliferation 0.5-1.4-fold compared with negative controls,whereas no effects were observed with P60S1-1.SHEN Xuexiang YANG Yan ZHANG Jinsong TANG Qingjiu JIA Wei 2008食用菌学报2008,15,2:2
15An Ultra Low Steady-State Current Power-on-Reset Circuit in 65nm CMOS Technology显示文摘A novel Power-on-reset(POR) circuit is proposed with ultra-low steady-state current consumption. A band-gap voltage comparator is used to generate a stable pull-up voltage. To eliminate the large current consumptions of the analog part, a power switch is adopted to cut the supply of band-gap voltage comparator, which gained ultra-low current consumption in steady-state after the POR rest process completed. The state of POR circuit is maintained through a state latch circuit. The whole circuit was designed and implemented in 65 nm CMOS technology with an active area of 120μm*160μm. Experimental results show that it has a steady pull-up voltage of 0.69 V and a brown-out voltage of 0.49 V under a 1.2V supply voltage rising from 0V, plus its steady-state current is only9 n A. The proposed circuit is suitable to be integrated in system on chip to provide a reliable POR signal.SHAN Weiwei WANG Xuexiang LIU Xinning SUN Huafang 2014Chinese Journal of Electronics2014,23,4:2
16Shallow Structure of the Crust in the Sulu-Dabie Region,China and its Seismotectonic Implication显示文摘The P-wave velocity structure in the shallow crust is investigated in and around the Sulu-Dabie region by using seismic reflection data for deep soundings in 48 survey profiles and from rock velocity determinations.The observed velocity distributions show obvious heterogeneities in this region.The low velocity anomalies are observed mainly in the west of the Dabie region and the East Sea regions.The high velocity anomalies emerge in the shallow crust of the Sulu and Dabie orogeny.These high-velocity anomalies can be attributed to the ultra-high pressure metamorphosed(UHPM)rock formed by exhumation motion of mantle materials during the orogeny.The high-velocity anomalies in the different shallow layers beneath the Sulu region are located to the northeast of the Tan-Lu fault.The high-velocity anomalies beneath the Dabie region are located southwest of the Tan-Lu fault.Such a distribution pattern of velocity anomaly zones may reveal historical motion of a left-lateral strike-slip for the Tan-Lu fault,which differs from the result of a right-lateral strike-slip motion regime known from modern seismology,indicating a more complex tectonic motion along the Tan-Lu fault.XU Jiren LI Haibing QI Xuexiang ZHAO Zhixin 2021Acta Geologica Sinica(English Edition)2021,95,3:2
17A novel synthetic small molecule YF-452 inhibits tumor growth through antiangiogenesis by suppressing VEGF receptor 2 signaling显示文摘Tumor angiogenesis is characterized by abnormal vessel morphology, endowing tumor with highly hypoxia and unresponsive toward treatment. To date, mounting angiogenic factors have been discovered as therapeutic targets in antiangiogenic drug development. Among them, vascular endothelial growth factor receptor 2 (VEGFR2) inhibitors exerts potent antiangiogenic activity in tumor therapy. Therefore, it may provide a valid strategy for cancer treatment through targeting the tumor angiogenesis via VEGFR2 pathway. In this study, we established a high-profile compounds library and certificated a novel compound named N-(N-pyrrolidylacetyl)-9-(4-bromobenzyl)-1,3,4,9-tetrahydro-β-carboline (YF-452), which remarkably inhibited the migration, invasion and tube-like structure formation of human umbilical vein endothelial cells (HUVECs) with little toxicity invitro. Rat thoracic aorta ring assay indicated that YF-452 significantly blocked the formation of microvascular exvivo. In addition, YF-452 inhibited angiogenesis in chick chorioallantoic membrane (CAM) and mouse corneal micropocket assays. Moreover, YF-452 remarkably suppressed tumor growth in xenografts mice model. Furthermore, investigation of molecular mechanism revealed that YF-452 inhibited VEGF-induced phosphorylation of VEGFR2 kinase and the downstream protein kinases including extracellular signal regulated kinase (ERK), focal adhesion kinase (FAK) and Src. These results indicate that YF-452 inhibits angiogenesis and may be a potential antiangiogenic drug candidate for cancer therapy.Yongrui Liu Yuan He Feifei Yang Xiaonan Cong Jinhua Wang Shihong Peng Dan Gao Weifang Wang Liping Lan Xuexiang Ying Mingyao Liu Yihua Chen Zhengfang Yi 2017Science China(Life Sciences)2017,60,2:2
18Degradation of selected pharmaceuticals in aqueous solution with UV and UV/H 2 O 2显示文摘Fang Yuan Chun Hu Xuexiang Hu Jiuhui Qu Min Yang 2009Water Research2009,,6:1
19Preparation and Visible- Light Activity of Silver Vanadate for the Degradation of Pollutants显示文摘Hu Xuexiang Hu Chun Qu Jiuhui 2008Mater Res Bull2008,43,11:1
20显示文摘Xu Ruifen Hu Xuexiang Hu Weikang 2003Chemical Research and Appficafion2003,15,5:1
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