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| 1 | Overexpression of the tonoplast aquaporin AtTIP5;1 conferred tolerance to boron toxicity in Arabidopsis显示文摘Boron (B) toxicity to plants is responsible for low crop productivity in many regions of the world. Here we report a novel and effective means to alleviate the B toxicity to plants under high B circumstance. Functional characterization of AtTIP5;1, an aquaporin gene, re-vealed that overexpression of AtTIP5;1 (OxAtTIP5;1) in Arabidopsis significantly increased its tolerance to high B toxicity. Compared to wild-type plants, OxAtTIP5;1 plants exhibited longer hypocotyls, accelerated development, increased silique production under high B treatments. GUS staining and quantitative RT-PCR (qRT-PCR) results demonstrated that the expression of AtTIP5;1 was induced by high B concentration treatment. Subcellular localization analysis revealed that the AtTIP5;1-GFP fusion protein was localized on the tonoplast membrane, which was consistent with the prediction based on bioinformatics. Taken together, our results suggest that AtTIP5;1 is in-volved in B transport pathway possibly via vacuolar compartmentation for B, and that overexpression of AtTIP5;1 in plants may provide an effective way to overcome the problem resulting from high B concentration toxicity. | Yongqi Pang, Lijuan Li, Fei Ren, Pingli Lu, Pengcheng Wei, Jinghui Cai, Lingguo Xin, Juan Zhang, Jia Chen, Xuechen Wang State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China | 2010 | Journal of Genetics and Genomics2010，37，6： | 11 |
| 2 | Molecular architecture of the luminal ring of the Xenopus laevis nuclear pore complex显示文摘The nuclear pore complex(NPC)mediates the flow of substances between the nucleus and cytoplasm in eukaryotic cells.Here we report the cryo-electron tomography(cryo-ET)structure of the luminal ring(LR)of the NPC from Xenopus laevis oocyte.The observed key structural features of the LR are independently confirmed by single-particle cryo-electron microscopy(cryo-EM)analysis.The LR comprises eight butterfly-shaped subunits,each containing two symmetric wings.Each wing consists of four elongated,tubular protomers.Within the LR subunit,the eight protomers form a Finger domain,which directly contacts the fusion between the inner and outer nuclear membranes and a Grid domain,which serves as a rigid base for the Finger domain.Two neighboring LR subunits interact with each other through the lateral edges of their wings to constitute a Bumper domain,which displays two major conformations and appears to cushion neighboring NPCs.Our study reveals previously unknown features of the LR and potentially explains the elastic property of the NPC. | Yanqing Zhang Sai Li Chao Zeng Gaoxingyu Huang Xuechen Zhu Qifan Wang Kunpeng Wang Qiang Zhou Chuangye Yan Wusheng Zhang Guangwen Yang Minhao Liu Qinghua Tao Jianlin Lei Yigong Shi | 2020 | Cell Research2020，30，6： | 4 |
| 3 | Identification of AtSM34, a novel tonoplast intrinsic protein-interacting polypeptide expressed in response to osmotic stress in germinating seedlings显示文摘Aquaporins are implicated in a wide variety of plant physiological processes, although the mechanisms involved in their regulation are not fully understood. To gain further insight into the regulatory factors involved in this process, we used a yeast two-hybrid system to screen for potential binding partners to the Arabidopsis tonoplast intrinsic protein (TIP) AtTIP1;1. This was the first protein identified to be associated with high water permeability in vacuolar membranes from Arabidopsis thaliana. Using AtTIP1;1 as bait, a novel binding protein was identified in both yeast and plant cells. This prey protein, named AtSM34, was a 309 aa polypeptide with a predicted molecular mass of 34 kD and contained a single MYB/SANT-like domain. AtSM34 promoter:: GUS histochemical staining analysis detected AtSM34 expression in flowers, stems and leaves, particularly in the vascular tissues, in response to osmotic stress. AtSM34 expression was localized in the endoplasmic reticulum membrane, and sequence deletion analysis revealed that the N-terminal coding region (amino acids 1-83) was critical for this localization. Overexpression of AtSM34 resulted in hypersensitivity to exogenous mannitol, sorbitol and abscisic acid, and caused a significant delay in germination. AtSM34 interacted with AtTIP1;2 and AtTIP2;1, which are essential proteins for modulation of tonoplast permeability and highly expressed in germinating seedlings. These data indicate AtSM34 is a novel TIPs binding protein involved in the osmotic stress response of seedlings at an early stage of development. | LI LiJuan REN Fei WEI PengCheng CHEN QiJun CHEN Jia WANG XueChen | 2011 | Chinese Science Bulletin2011，56，33： | 3 |
| 4 | Synthesis of Constrained Head‐to‐Tail Cyclic Tetrapeptides by an Imine‐Induced Ring‐Closing/Contraction Strategy显示文摘 | Clarence T. T. Wong Hiu Yung Lam Tao Song Guanhua Chen Xuechen Li | 2013 | Angew Chem Int Ed2013，，39： | 2 |
| 5 | High-performance all-polymer solar cells with only 0.47 eV energy loss显示文摘The field of all-polymer solar cells(all-PSCs)has experienced rapid development during the past few years,mainly driven by the development of efficient polymer acceptors.However,the power conversion efficiencies(PCEs)of the all-PSCs are still limited by insufficient light absorption of the donor/acceptor blend and large energy loss in devices.We herein designed a polymer acceptor PYT1 constructed n-type molecular acceptor Y5-C20 as the key building block and blended it with a polymer donor PM6 to obtain an all-polymer photoactive layer.The optimized PM6:PYT1 all-PSCs achieved a record higher PCE of 13.43%with a very low energy loss of 0.47 eV and a photoresponse of up to 900 nm compared with the Y5-C20 based device with a best PCE of 9.42%.Furthermore,the PCEs of the PM6:PYT1 all-PSCs are relatively insensitive to the 1-chloronaphthalene(CN)additive contents and active layer thickness.Our results also highlight the effect of CN additive on PM6:PYT1 morphology,i.e.,charge generation,and transport find an optimized balance,and radiative and non-radiative loss is simultaneously reduced in the blend.This work promotes the development of high-performance polymer acceptors and heralds a brighter future of all-PSCs for commercial applications. | Qiang Wu Wei Wang Tao Wang Rui Sun Jing Guo Yao Wu Xuechen Jiao Christoph J.Brabec Yongfang Li Jie Min | 2020 | Science China Chemistry2020，63，10： | 2 |
| 6 | Simultaneous Biological Removal of p-Cresol, Sulfide and Nitrate by Denitrification显示文摘Biological removal of sulfide, nitrate, and p-cresol at a loading rate of 340 gS/(m^3 · d), 340 gN/(m^3 · d) and600 gC/(m^3 · d), respectively, was achieved in an upflow anaerobic sludge blanket(UASB) reactor. The ratio of C/S could affect the elemental sulfur(S^0) accumulation, and the C/S ratio of 1.75:1 was optimal for S^0 accumulation. Strains Pseudomonas sp., Simplicispira sp., and Rhizobium sp. could likely yield heterotrophs. Strains Arcobacter sp.,Sulfurimonas sp., Sulfurovum sp., and Sulfurospirillum sp. are the autotrophics required for the proposed UASB system. At high loading rate, the autotrophic denitrification pathway declined faster than the heterotrophic pathway. | Liu Chunshuang Li Wenfei Zhao Dongfeng Li Wei Li Xuechen Zhao Chaocheng Liu Fang | 2017 | China Petroleum Processing & Petrochemical Technology2017，19，4： | 2 |
| 7 | Rapid Acclimation of Methanogenic Granular Sludge into Autotrophic Partial Denitrification Granules显示文摘Rapid formation of autotrophic partial denitrification(APD)granules is of practical interest to start up an expanded granular sludge bed reactor for wastewater treatment.This study demonstrates that methanogenic granules can be easily acclimated into autotrophic partial denitrification granules in one day,with the ability to remove 82%of 2.7 kg-S/(m^3·d)sulfide into S^0 and to convert 97%of 0.9 kg-N/(m^3·d)nitrate into nitrite,which can provide a promising feedstock for anaerobic ammonia oxidation process.Arcobacter sp.is essential for S^0 accumulation.Under high loadings,the abundance of Arcobacter sp.decreased,while on the contrary the abundance of unclassified_p_Firmicutes increased,leading to the deterioration of autotrophic partial denitrification performance.The granules performance could be recovered by adopting the strategies of properly reducing the influent loadings. | Liu Chunshuang Li Xuechen Zhang Xiaofei Bai Xue Guo Yadong Wang Yongxing Zhao Chaocheng | 2019 | China Petroleum Processing & Petrochemical Technology2019，21，3： | 2 |
| 8 | 查看详情显示文摘 | Li Xuechen Zhao Na Fang Tongzhen | | 0，，： | 1 |
| 9 | Biodegradable polylactide/poly(ethylene glycol)/ polylactide triblock copolymer micelles as anticancer drug carriers显示文摘 | LIU Li LI Chenxi Li LI Xuechen | 2001 | J of Applied Polymer Science2001，80，： | 1 |
| 10 | An Adaptive Method for Identifying the Spatial Patterns in Road Networks显示文摘 | YANG BISHENG LUAN XUECHEN LI QINGQUAN | | 0，，01： | 1 |
| 11 | DGP1,a drought-induced guard cell-specific promoter and its function analysis in tobacco plants显示文摘The genetic regulation of stomatal movement mainly depends on an efficient control system of gene expression, and guard cell-specific promoter is becoming the best choice. Here we combined the dehydration responsive element (DRE) with guard cell specific element (GCSE) to construct a novel promoter, DGP1. Histochemical assays in transgenic tobacco carrying β -glucuronidase (gus) gene fused to DGP1 demonstrated that GUS activity was found to be highly inducible by drought treatment and specifically restricted to guard cells. No GUS activity was detected in roots, stems or flowers after treatment. Further quantitative analysis showed that GUS activity in the epidermal strips was apparently induced by dehydration and dramatically increased with the elongation of treatment. The GUS activity after 8 h treatment was 179 times that of those without treatment. Although GUS activity in roots, stems or mesophyll increased after treatment, no great changes were observed. These results suggested that DGP1 could drive target gene expressed in guard cells when plant is subjected to drought stress. And this gets us prepared to control opening and closing of stomata through plant gene engineering. | LI Jun GONG Ximing LIN Huiqiong SONG Quanbo CHEN Jia WANG Xuechen | 2005 | Science China(Life Sciences)2005，48，2： | 1 |
| 12 | Development of aspartic acid ligation for peptide cyclization derived from serine/threonine ligation显示文摘Based on a mechanism analogous to the serine/threonine ligation, the aspartic acid ligation, which is facilitated by the γ-amino alcohol based ligation and oxidation, is developed and applied to the synthesis of cyclic peptides. The γ-hydroxyl group triggers the ring-chain tautomerization via a 6-endo-trig process,while the δ-hydroxyl group facilitates the oxidative cleavage of the vicinal diol to give carboxylic acid. | Ci Xu Jianchao Xu Han Liu Xuechen Li | 2018 | Chinese Chemical Letters2018，29，7： | 1 |
| 13 | Shorter alkyl chain in thieno[3,4-c]pyrrole-4,6-dione(TPD)-based large bandgap polymer donors – Yield efficient non-fullerene polymer solar cells显示文摘Typically,conjugated polymers are composed of conjugated backbones and alkyl side chains.In this contribution,a cost-effective strategy of tailoring the length of alkyl side chain is utilized to design highperforming thieno[3,4-c]pyrrole-4,6-dione(TPD)-based large bandgap polymer donors PBDT-BiTPD(Cχ)(χ=48,52,56),in which x represents the alkyl side chain length in term of the total carbon number.A combination of light absorption,device,and morphology examinations make clear that the shorter alkyl side chains yield(i) higher crystallinity and more predominant face-on crystallite orientation in their neat and BHJ blend films,(ii) higher charge mobilities(6.7×10^(-4) cm~2 V^(-1) s^(-1) for C48 vs.3.2×10^(-4) cm~2 V^(-1) s^(-1) for C56),and negligible charge recombination,consequently,(iii) significantly improved fill-factor(FF) and short current(J_(SC)),while almost the same open circuit voltage(V_(OC)) of ca.0.82 V in their corresponding BHJ devices.In parallel,as alkyl side chain lengths decrease from C56 to C48,power conversion efficiencies(PCEs) increased from 7.8% for C56 to 11.1% for C52,and further to14.1% for C48 in their BHJ solar cells made with a narrow bandgap non-fullerene acceptor Y6.This systematic study declares that shortening the side chain,if providing appropriate solubility in device solution processing solvents,is of essential significance for developing high-performing polymer donors and further improving device photovoltaic performance. | Jiaji Zhao Xuelong Huang Qingduan Li Shengjian Liu Ziqiang Fan Di Zhang Shanshan Ma Zhixiong Cao Xuechen Jiao Yue-Peng Cai Fei Huang | 2021 | Journal of Energy Chemistry2021，30，2： | 1 |
| 14 | A bistable Switching of Two-dimensional Photonic Crystal with Kerr Point Defect 显示文摘 | LI XUECHEN DALWOO KIM | 2003 | Opt Commun2003，218，1： | 1 |
| 15 | Generating hierarchical strokes from urban street networks based on spatial pattern recognition显示文摘 | Bisheng Yang Xuechen Luan Qingquan Li | 2011 | International Journal of Geographical Information Science2011，，12： | 1 |
| 16 | Research progress in the study of atmospheric pressure glow barrier discharge显示文摘Atmospheric pressure glow barrier discharge (APGBD) can operate at high pressure, and so vacuum device is not necessary. Furthermore, the produced plasma by APGBD has moderate electron temperature and density besides good uniformity. Therefore, APGBD has extensive potential applications in industry and has been becoming a hot issue in the research of low temperature plasma. In this paper, the main problems in the study of atmospheric pressure glow discharge generated by dielectric barrier discharge, including the experimental setup, judging criterion, discharging conditions, physical mechanisms, and parameter diagnoses, are discussed, and further research prospects of APGBD are proposed. | LI Xuechen DONG Lifang JIA Pengying | 2007 | Progress in Natural Science:Materials International2007，17，3： | 1 |
| 17 | Studies on 2-Formylphenylboronic Acid-Based Ser/Thr Ligation and Cys/Pen Ligation显示文摘Main observation and conclusion Developing an effective chemoselective ligation strategy for assembling unprotected peptide segments is important to peptide/protein synthesis.The ligation efficiency and selectivity depend on the chemistry between the C-terminal active ester of the peptide(e.g.,the thioester in Native Chemical Ligation or the salicylaldehyde ester in Ser/Thr Ligation and Cys/Pen Ligation)and N-terminal functional groups of the second peptide(Cys or Ser/Thr).Increasing the reactivity of the ligation and decreasing the hydrolysis of the active ester are more like the opposite sites in balance.We investigated the potential application of the peptide acyl ester of 2-formylphenylboronic acid(2-FPBA)in the Ser/Thr ligation and Cys/Pen ligation.With the assistance of boronic acid group,the peptidyl FPBA ester showed remarkable stability in aqueous buffer and its reactivity towards Ser/Thr and Cys/Pen also improved.However,the strong coordination of the amine at the N-terminus and boronic acid suppressed the acyl transfer process when the peptide acyl group was used.More effects should be made to enhance the ligation chemistry. | Zhenquan Sun Xuechen Li | 2021 | Chinese Journal of Chemistry2021，39，10： | 1 |
| 18 | An A- daptive Method for Identifying the Spatial Patterns in Road Networks 显示文摘 | Yang Bisheng Luan Xuechen Li Qingquan | 2010 | Computers Environment and Urban Systems2010，34，： | 1 |
| 19 | Effects of ammonium chloride and heat treatment on residual formalde- hyde contents of melamine-formaldehyde microcapsules显示文摘 | Li Wei Wang Jianping Wang Xuechen | 2007 | Colloid Polymer Science2007，285，15： | 1 |
| 20 | An Adaptive Method for Identifying the Spatial Patterns in Road Networks 显示文摘 | YANG Bisheng LUAN Xuechen LI Qingquan | 2010 | Computers Environment and Urban Systems2010，34，1： | 1 |
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