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| 1 | The mechanism of the flowing ground water impacting on coalbed gas content显示文摘The hydrogeological condition affects the coal-bed gas storage dramatically. In an area of stronger hydrodynamics, the coal has a lower gas content, while a higher gas content exists in an area of weaker hydrodynamics. Obviously, the flowing groundwater is harmful to coalbed gas preservation. But few researches focus on the mechanism of how the flowing water diminishes the coalbed gas content Based on the phenomenon that the flowing groundwater not only makes coalbed gas content lower, but also fractionates the carbon isotope, this research puts forward an idea that it is the water solution that diminishes the coalbed gas content, rather than the water-driven action or the gas dissipation through cap rocks. Only water-soluble action can both fractionate the carbon isotope and lessen the coalbed gas content, and it is an efficient way to take gas away and affect the gas content. | QIN Shengfei SONG Yan TANG Xiuyi FU Guoyou | 2005 | Chinese Science Bulletin2005，50，S1： | 3 |
| 2 | Immunogenicity and tumor-inhibiting effects of HSP-antigen peptide complex in melanoma B16 cells in mice显示文摘目的 :黑色素瘤 B1 6细胞热休克蛋白 -抗原肽复合物 (HACs)及其粗提物 (HAC- CEs)的制备 ,以及它们的免疫原性和抑瘤效应的研究。方法 :应用 Sephacryl S- 2 0 0凝胶过滤制备HAC- CEs,应用亲和层析纯化 HACs,并测其免疫功能和抑瘤效应。结果 :应用凝胶过滤制备的HAC- CE3、HAC- CE4、HAC- CE5和应用亲和层析纯化的 HAC60 ,HAC75和 HAC97均不同程度地降低肿瘤发生率、延迟肿瘤发生时间和减少移植黑色素瘤 C57BL/6J小鼠死亡率 ;同时 ,伴有小鼠脾细胞 IFN-γ和 IL- 2分泌活性及 CTL杀伤率的增加。结论 :分子量为 60 0 0 0~ 970 0 0的 HACs具有免疫原性和抑瘤效应 ,本研究为制备肿瘤疫苗提供重要的实验依据。 | Gong Shouliang Yang Ying Fu Shibo Li Xiujuan Sun Zuyue Li Xiuyi | 2000 | 白求恩医科大学学报2000，26，5： | 3 |
| 3 | Genetic dissection of carotenoids in maize kernels using high-density single nucleotide polymorphism markers in a recombinant inbred line population显示文摘Carotenoids are antioxidants and vitamin A precursors that have important roles in human health. Hence, improving the carotenoid contents in maize kernels is a priority objective for breeders in order to obtain nutritional biofortification outcomes. In the current study, the genetic architecture of carotenoids in maize kernels was explored using a recombinant inbred line(RIL) population derived from a cross between inbred lines By804 and B73. A total of 81 QTLs were detected by using a high-density bin map and a simple sequence repeat(SSR)-based linkage map, with one to seven QTLs for each trait explaining 4.21%–47.53% of the phenotypic variation. A comparison of the QTL mapping efficiency between the two linkage maps revealed that the high-density bin map had higher resolution. In the current study 46 additional QTLs were identified, with 16 being common with previous studies and14 newly identified. Among the results, 29.6%(24/81) of QTLs explained > 10% of the phenotypic variation in the RIL population, and 70.4%(57/81) explained ≤ 10%. These results suggest that a few large-effect QTLs, together with a variable number of minor-effect QTLs,contributed to most of the genetic components of carotenoids in maize kernels. | Orawan Jittham Xiuyi Fu Jing Xu Subhash Chander Jiansheng Li Xiaohong Yang | 2017 | The Crop Journal2017，5，1： | 1 |
| 4 | Genetic architecture of embryo size and related traits in maize显示文摘The embryo in maize has a critical role in controlling kernel nutrition components and grain yield.We measured five embryo weight and size traits,six kernel weight and size traits,and five embryo-tokernel ratio traits in a nested association mapping(NAM)population of 611 recombinant inbred lines(RILs)derived from four inbred lines including the high-oil,giant-embryo line BY815 as the common parent.Using three statistical methods,we identified 5–22 quantitative trait loci(QTL)for each trait,explaining 4.7%–46.7%of the phenotypic variation.The genetic architecture of maize embryo size and its related traits appeared to be dominated by multiple small-effect loci with little epistasis,and the genetic control underlying embryo size appeared to be distinct from that underlying kernel size.A trait–QTL association network included 205 nodes and 439 edges and revealed 28 key loci associated with at least three traits.Cloned maize genes including Zm Urb2,Emp12 and Zm BAM1 d,maize orthologs of known rice genes that control seed size including BG1,XIAO and GS9,and 11 maize orthologs of Arabidopsis EMBRYO-DEFECTIVE(EMB)genes were identified as underlying these key loci.Further,the phenotypic and genetic relationships between embryo size and kernel size were evaluated,and genetic patterns for identified loci that control embryo size and its related traits were proposed.Our findings reveal distinct genetic architectures for embryo size,kernel size,and embryo-to-kernel ratio traits and establish a foundation for the improvement of embryo-size-mediated kernel nutrition and grain yield. | Xiaowei Li Min Wang Renyu Zhang Hui Fang Xiuyi Fu Xiaohong Yang Jiansheng Li | 2022 | The Crop Journal2022，10，1： | 1 |
| 5 | Integrated linkage mapping and genome-wide association study to dissect the genetic basis of zinc deficiency tolerance in maize at seedling stage显示文摘Zinc(Zn)deficiency is the most widespread micronutrient deficiency,affecting yield and quality of crops worldwide.Identifying genes associated with Zn-deficiency tolerance in maize is a basis for elucidating its genetic mechanism.A K22×CI7 recombinant inbred population consisting of 210 lines and an association panel of 508 lines were used to identify genetic loci influencing Zn-deficiency tolerance.Under-Zn and-Zn/CK conditions,15 quantitative trait loci(QTL)were detected,each explaining 5.7%-12.6%of phenotypic variation.Sixty-one significant single-nucleotide polymorphisms(SNPs)were identified at P<10^(-5)by genome-wide association study(GWAS),accounting for 5%-14%of phenotypic variation.Among respectively 198 and 183 candidate genes identified within the QTL regions and the 100-kb regions flanking these significant SNPs,12 were associated with Zn-deficiency tolerance.Among these candidate genes,four genes associated with hormone signaling in response to Zn-deficiency stress were co-localized with QTL or SNPs,including the genes involved in the auxin(ZmARF7),and ethylene(ZmETR5,ZmESR14,and ZmEIN2)signaling pathways.Three candidate genes were identified as being responsible for Zn transport,including ZmNAS3 detected by GWAS,ZmVIT and ZmYSL11 detected by QTL mapping.Expression of ZmYSL11 was up-regulated in Zn-deficient shoots.Four candidate genes that displayed different expression patterns in response to Zn deficiency were detected in the regions overlapping peak GWAS signals,and the haplotypes for each candidate gene were further analyzed. | Jianqin Xu Zhongfu Ni Fanjun Chen Xiuyi Fu Futong Yu | 2022 | The Crop Journal2022，10，6： | 0 |
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