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| 1 | Resveratrol provides neuroprotection by regulating the JAK2/STAT3/PI3K/AKT/mTOR pathway after stroke in rats显示文摘Ischemic stroke is a common disease with high mortality and morbidity worldwide.One of the important pathophysiological effects of ischemic stroke is apoptosis.A neuroprotective effect is defined as the inhibition of neuronal apoptosis to rescue or delay the infarction in the surviving ischemic penumbra.Resveratrol is a natural polyphenol that reportedly prevents cerebral ischemia injury by regulating the expression of PI3K/AKT/mTOR.Therefore,this study aimed to elucidate the neuroprotective effect of resveratrol on cerebral ischemia/reperfusion injury and to investigate the signaling pathways and mechanisms through which resveratrol regulates apoptosis in the ischemic penumbra.Rats were subjected to middle cerebral artery occlusion for 2 h followed by 24 h reperfusion.Cerebral infarct volume was measured using 2%TTC staining.TUNEL staining was conducted to evaluate neuronal apoptosis.Western blotting and immunohistochemistry were used to detect the proteins involved in the JAK2/STAT3/PI3K/AKT/mTOR pathway.The results suggested that resveratrol significantly improved neurological function,reduced cerebral infarct volume,decreased neuronal damage,and markedly attenuated neuronal apoptosis;these effects were attenuated by the inhibition of PI3K/AKT with LY294002 and JAK2/STAT3 with AG490.We also found that resveratrol significantly upregulated the expression of p-JAK2,p-STAT3,p-AKT,p-mTOR,and BCL-2 and downregulated expression of cleaved caspase-3 and BAX,which was partially reversed by LY294002 and AG490.These results suggested that resveratrol provides a neuroprotective effect against cerebral ischemia/reperfusion injury,which is partially mediated by the activation of JAK2/STAT3 and PI3K/AKT/mTOR.Resveratrol may indirectly upregulate the PI3K/AKT/mTOR pathway by activating JAK2/STAT3. | Yongying Hou Ke Wang Weijun Wan Yue Cheng Xia Pu Xiufeng Ye | 2018 | Genes & Diseases2018，5，3： | 60 |
| 2 | Integrated Power Generation Scheduling Compilation Method for Large-scale Small Hydropower Station Group显示文摘针对小水电无序管理,弃水、窝电现象严重,水资源浪费问题突出的现状,设计省地一体化小水电发电计划编制方法。该方法规范了小水电计划编制流程,对发电计划实行分级制作与管理,增加了输电断面安全校核环节,并给出了超限断面计划调整方法,通过建议计划上报一输电断面校核一计划协调发布的闭环方式有效提高了发电计划的准确性和可操作性。该方法目前已应用于云南电网小水电管理系统,方法的科学性与有效性得到了实际工程的验证。 | LI Shushan LIAO Shengli LI Gang CHENG Chuntian CAI Huaxiang LI Xiufeng | 2012 | 中国电机工程学报2012，32，13： | 15 |
| 3 | Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology显示文摘Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation. | Xuchao Zhang Zhiyong Liang Shengyue Wang Shun Lu Yong Song Ying Cheng Jianming Ying Weiping Liu Yingyong Hou Yangqiu Li Yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu Jinji Yang Wenzhao Zhong Qing Zhou Xuening Yang Jie Wang Cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian Yu Chen Ruibao Ren Ning Liao Chunyan Wu Zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang Yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao Binghe Xu Qingyuan Zhang Ruihua Xu Lin Shen Yilong Wu | 2019 | Cancer Biology & Medicine2019，16，1： | 14 |
| 4 | Evidence of compliance with and effectiveness of guidelines for noninvasive prenatal testing in China:a retrospective study of 189,809 cases显示文摘In China,the medical guidelines recommend performing noninvasive prenatal testing(NIPT)with caution for pregnant women aged 35 years or older.However,the Mother and Child Health Care Law suggests that all primiparous women whose age is older than 35 years undergo prenatal diagnosis.These two inconsistent suggestions/recommendations have made obstetricians confused about whether to offer NIPT to these older pregnant women.To face this issue and find out the solution we performed a retrospective study of 189,809 NIPT samples collected from 28 provincial-leveled administrative units in China.Of 1,564 women with high-risk pregnancies who underwent NIPT,459(29.3%)did not participate in follow-up.The compound sensitivity and specificity of NIPT for trisomies 21,18 and 13 detection was 99.1%(95%CI,98.0%–99.6%)and 99.9%(95%CI,98.8%–99.9%),respectively.In secundiparous women,NIPT showed high sensitivity and specificity similar to that in primiparous women.The observed risk for trisomies 21 and 18 significantly increased when the maternal age was 39 and older.After the publication of the current NIPT policy,the follow-up rate at our center was 97.9%;however,a large number of women are not in maternal and infant care networks nationwide,and that makes the follow-up rate outside our center relatively low.Our study shows that to balance the prevention of major aneuploidies and the limited resources for prenatal diagnosis,the cut-off age of 35 for invasive prenatal diagnosis might be unnecessary.Although the NIPT guidelines are well written,how to practice it effectively,especially in less industrialized areas,is worth discussing. | Chan Tian Tao Deng Xiuhuang Zhu Chen Gong Yangyu Zhao Yuan Wei Rong Li Xiufeng Xu Miaonan He Zhiwei Zhang Jing Cheng Mol BenWillem Jie Qiao | 2020 | Science China(Life Sciences)2020，63，3： | 10 |
| 5 | Preparation of modified SiO_2 colloidal spheres with succinic acid and the assembly of colloidal crystals显示文摘SiO2 colloidal spheres were synthesized by St-ber method. In order to enhance surface charge of the SiO2 spheres, they were modified with succinic acid. Scanning electron microscope (SEM) shows that the average size of modified SiO2 spheres is 473 nm, and its distribution standard deviation is less than 5%; Fourier-transform infrared spectra (FT-IR) and X-ray photoelectron spectrometer (XPS) results indicate that one end of succinic acid is chemically bonded to the SiO2 spheres through esterification; Zeta potential of the modified SiO2 spheres in water solution is improved from -53.72 to -67.46 mV, and surface charge density of the modified SiO2 spheres is enhanced from 0.19 to 0.94 μC/cm2. SiO2 colloidal crystal was fabricated from aqueous colloidal solution by the vertical deposition method at 40℃ and 60% relative humidity. SEM images show that the sample of SiO2 colloidal crystal is face-centered cubic (fcc) structure with its (111) planes parallel to the substrate. Transmission measurement shows the existence of photonic band gap at 1047 nm. | FANG Jun WANG XiuFeng WANG LieSong CHENG Bing WU Yuan-Ting ZHU WanLin | 2007 | Chinese Science Bulletin2007，52，4： | 9 |
| 6 | Salmonella flagella confer anti-tumor immunological effect via activating Flagellin/TLR5 signalling within tumor microenvironment显示文摘Salmonella:mediated cancer therapy has achieved remarkable anti-tumor effects in experimental animal models,but the detailed mechanism remains unsolved.In this report,the active involvement of the host immune response in this process was confirmed by comparing the tumor-suppressive effects of Salmonella in immunocompetent and immunodeficient mice bearing melanoma allografts.Since flagella are key inducers of the host immune response during bacterial infection,flagella were genetically disrupted to analyse their involvement in Salmonella-mediated cancer therapy.The results showed that flagellum-deficient strains failed to induce significant anti-tumor effects,even when more bacteria were administered to offset the difference in invasion efficiency.Flagella mainly activate immune cells via Flagellin/Toll-like receptor 5(TLR5)signalling pathway.Indeed,we showed that exogenous activation of TLR5 signalling by recombinant Flagellin and exogenous expression of TLR5 both enhanced the therapeutic efficacy of flagellum-deficient Salmonella against melanoma.Our study highlighted the therapeutic value of the interaction between Salmonella and the host immune response through Flagellin/TLR5 signalling pathway during Salmonella-mediated cancer therapy,thereby suggesting the potential application of TLR5 agonists in the cancer immune therapy. | Jianxiang Chen Yiting Qiao Guo Chen Cunjie Chang Heng Dong Bo Tang Xiawei Cheng Xiufeng Liu Zichun Hua | 2021 | Acta Pharmaceutica Sinica B2021，11，10： | 6 |
| 7 | Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study显示文摘Emerging evidence suggests that children conceived through assisted reproductive technology(ART)have a higher risk of congenital heart defects(CHDs)even when there is no family history.De novo mutation(DNM)is a well-known cause of sporadic congenital diseases;however,whether ART procedures increase the number of germline DNM(gDNM)has not yet been well studied.Here,we performed whole-genome sequencing of 1137 individuals from 160 families conceived through ART and 205 families conceived spontaneously.Children conceived via ART carried 4.59 more gDNMs than children conceived spontaneously,including 332 paternal and 1.26 maternal DNMs,after correcting for parental age at conception,cigarette smoking,alcohol drinking,and exercise behaviors.Paternal DNMs in offspring conceived via ART are characterized by C>T substitutions at CpG sites,which potentially affect protein-coding genes and are significantly associated with the increased risk of CHD.In addition,the accumulation of non-coding functional mutations was independently associated with CHD and 87.9% of the mutations were originated from the father.Among ART offspring,infertility of the father was associated with elevated paternal DNMs;usage of both recombinant and urinary follicle-stimulating hormone and high-dosage human chorionic gonadotropin trigger was associated with an increase of maternal DNMs.In sum,the increased gDNMs in offspring conceived by ART were primarily originated from fathers,indicating that ART itself may not be a major reason for the accumulation of gDNMs.Our findings emphasize the importance of evaluating the germline status of the fathers in families with the use of ART. | Cheng Wang Hong Lv Xiufeng Ling Hong Li Feiyang Diao Juncheng Dai Jiangbo Du Ting Chen Qi Xi Yang Zhao Kun Zhou Bo Xu Xiumei Han Xiaoyu Liu Meijuan Peng Congcong Chen Shiyao Tao Lei Huang Cong Liu Mingyang Wen Yangqian Jiang Tao Jiang Chuncheng Lu Wei Wu Di Wu Minjian Chen Yuan Lin Xuejiang Guo Ran Huo Jiayin Liu Hongxia Ma Guangfu Jin Yankai Xia Jiahao Sha Hongbing Shen Zhibin Hu | 2021 | Cell Research2021，31，8： | 2 |
| 8 | Preparation of SiO_2 microspheres with sodium ions and self-assembly of photonic crystals显示文摘SiO2 microspheres were prepared via a novel co-precipitation process.The Na + ions were introduced by adding sodium chloride and metal sodium in solution systems for improving surface charge of the SiO2 microspheres.And then SiO2 photonic crystals were fabricated by the vertical deposition method.The electrical properties,micromorphology and optical properties were characterized by zeta potential instrument,scanning electron microscope (SEM) with energy dispersive X-ray spectroscopy and Uv-Vis-Nir spectrophotometer.Zeta potential of the SiO2 microspheres modified by sodium chloride and metal sodium were averagely heightened 11.39 mV and 13.52 mV respectively in ethanol solution.EDS spectra showed that there was Na element in the SiO2 microspheres.SEM images showed that the average size of the SiO2 microspheres modified by sodium chloride and metal sodium were 380 nm and 354 nm respectively,larger than that of the unmodified ones,and their distribution standard deviation was less than 5%,and the sample of SiO2 photonic crystals was face-centered cubic structure with their (111) planes parallel to the substrate.In addition,absorption spectra showed that photonic band gap of the SiO2 photonic crystals was modified by sodium chloride and metal sodium located in 856 nm and 798 nm respectively.Meanwhile,their absorption peaks became narrower and higher and exhibited a red-shift phenomenon. | LIU LiYing WANG XiuFeng ZHANG ChunXiang CHENG Bing | 2010 | Chinese Science Bulletin2010，55，8： | 2 |
| 9 | The growth and properties of Ca3TaGa3Si2O14 single crystals 显示文摘 | WANG Zeng-mei YUAN Duo-rong CHENG Xiufeng | 2003 | J Cryst Grwoth2003，253，3： | 1 |
| 10 | Growth, thermal and optical properties of Sr3NbGa3Si2O14 single crystals 显示文摘 | WANG Zeng-mei YUAN Duo-rong CHENG Xiufeng | 2003 | J Cryst Grwoth2003，258，4： | 1 |
| 11 | Genomic decoding of breeding history to guide breeding-by-design in rice显示文摘Deciphering the intrinsic molecular logic of empirical crop breeding from a genomic perspective is a decisive prerequisite for breeding-by-design(BbD),but remains not well established.Here,we decoded the historical features of past rice breeding by phenotyping and haplotyping 546 accessions covering the majority of cultivars bred in the history of Northeast China(NEC).We revealed that three groups founded the genetic diversities in NEC rice with distinct evolution patterns and traced and verified the breeding footprints to known or genome-wide association study(GWAS)-detected quantitative trait loci(QTLs),or introgressions from indica sub-species with chronological changes in allele frequencies.Then we summarized a rice breeding trend/principle in NEC,and combined with the successful example in breeding and application of Zhongkefa5 to demonstrate the guiding value of our conclusion for BbD in practice.Our study provides a paradigm for decoding the breeding history of a specific crop to guide BbD,which may have implications in different crop breeding. | Zhuo Chen Qingyun Bu Guifu Liu Maoqing Wang Hongru Wang Huazhao Liu Xiufeng Li Hong Li Jun Fang Yan Liang Zhenfeng Teng Sai Kang Hong Yu Zhukuan Cheng Yongbiao Xue Chengzhi Liang Jiuyou Tang Jiayang Li Chengcai Chu | 2023 | National Science Review2023，10，5： | 1 |
| 12 | Crystal Growth and Properties of Ca3NbGa3Si2O14 Single Crystals显示文摘 | Wang Zengmei Cheng Xiufeng Yuan Duorong | 2003 | Journal of Crystal Growth2003，249，： | 1 |
| 13 | Fuzzy SVM with anew fuzzy membership function显示文摘 | Xiufeng Jiang Zhang Yi Jian Cheng Lü | 2006 | Neural Computing andApplications2006，15，34： | 1 |
| 14 | Surface modification of sub-micron spherical SiO2 particles with butanedioic acid for electrophoresis in tetrachloroethylene显示文摘 | Cheng Bing Wang Xiufeng Wang Liesong | 2007 | Mater Lett2007，61，6： | 1 |
| 15 | Thermal and laser properties of Yb:YAl3(BO3)4 crystal显示文摘 | Li Jing Wang Jiyang Cheng Xiufeng Hu Xiaobo Burns P A Dawes J M | 2003 | Journal of Crystal Growth2003，250，34： | 1 |
| 16 | Growth and thermal properties of YbVO4 single crystal显示文摘 | Yu Yonggui Cheng Yan Zhang Huaijin Wang Jiyang Cheng Xiufeng Xia Hairui | 2006 | Materials Letters2006，60，： | 1 |
| 17 | Crystal Growth and Properties of Ca3NbGa3Si2O14 Single Crystals 显示文摘 | Wang Zengmei Cheng Xiufeng Yuan Duorong | 2003 | Journal of Crystal Growth2003，249，： | 1 |
| 18 | Growth and Characterization of Sr3NbGa3Si2O14 Single Crystals 显示文摘 | Wang Zengmei Yuan Duorong Cheng Xiufeng | 2003 | Journal of Crystal Growth2003，252，： | 1 |
| 19 | The Growth and Properties of Ca3TaGa3Si2O14 Single Crystals 显示文摘 | Wang Zengmei Yuan Duorong Cheng Xiufeng | 2003 | Journal of Crystal Growth2003，253，： | 1 |
| 20 | Growth, Thermal and Optical Properties of Sr3NbGa3Si2O14 Single Crystals 显示文摘 | Wang Zengmei Yuan Duorong Cheng Xiufeng | 2003 | Journal of Crystal Growth2003，258，： | 1 |
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