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| 1 | The Genome of Medicinal Plant Macleaya cordata Provides New Insights into Benzylisoquinoline Alkaloids Metabolism显示文摘在动物农业和药的抗菌素的 overuse 引起了一系列潜在的威胁到公共健康。Macleaya cordata 是从 Papaveraceae 家庭的药用的植物种,提供为抗菌剂的制造的一个安全资源为家畜喂添加剂。从 M. 的活跃成分 ? cordata 被知道包括 benzylisoquinoline 碱(偏爱) 象 sanguinarine (圣) 和 chelerythrine (CHE ) 那样的 ?,而是他们的新陈代谢的小径还得在这非模型植物被学习。在 M. 的圣和 CHE 的活跃生合成 ? cordata 被喂 13标记 C 的酷氨酸。为了增加,推进卓见,我们 de novo 定序 M 的整个染色体。cordata,第一从 Papaveraceae 家庭被定序。M.? 盖住 378 Mb 的 cordata 染色体与 43.5%being 编码 22,328 预言的编码蛋白质的基因 transposable 元素。作为基础 eudicot 的一个成员, M.? cordata 染色体缺乏 ? 发生在几乎所有 eudicots 的 paleohexaploidy 事件。从 genomics 数据, 16 的一个完全的集合 ? 新陈代谢 ? 为圣和 CHE 生合成的基因被检索,并且 14 项他们的生物化学的活动被验证。这些 genomics 和新陈代谢的数据在 M. 显示出保存 BIA 新陈代谢的小径 ? cordata 并且由庄稼改进或微生物引起的小径重建为圣和 CHE 的未来生产提供知识基础。 | Xiubin Liu Yisong Liu Peng Huang Yongshuo Ma Zhixing Qing Qi Tang Huifen Cao Pi Cheng Yajie Zheng Zejun Yuan Yuan Zhou Jinfeng Liu Zhaoshan Tang Yixiu Zhuo Yancong Zhang Linlan Yu Jialu Huang Peng Yang Qiong Peng dinbo Zhang Wenkai Jiang Zhonghua Zhang Kui Lin Dae-Kyun Ro Xiaoya Chen Xingyao Xiong Yi Shang Sanwen Huang Jianguo Zeng | 2017 | Molecular Plant2017，10，7： | 19 |
| 2 | Spatial patterns of urban land use growth in Beijing显示文摘By using digitized land use maps of Beijing in 1982, 1992 and 1997 and employing GIS spatial analysis techniques, this paper conducts an empirical study on the spatial differentiation and spatial patterns of urban land use growth in Beijing in the period of 1982-1997. It is observed that urban land use growth in Beijing went beyond the control of urban planning, in terms of the extraordinary high growth rate and undesired spatial pattern. The rate of urban expansion after 1982, which was predominated by growth of industrial land, was extraordinary high compared to its historical period. While its growth centers have been actively shifting toward the northern part, rather than toward the southern and eastern parts as designated by the latest General Plan (1991-2010) of Beijing, its spatial pattern of urban land use growth in general was in distinct concentric sprawl, which seriously violated the General Plan of Beijing. | LIU Shenghe, Sylvia Prielei, LI Xiubin(1. Inst. of Geographic Sciences and Natural Resources Research, CAS, Beijing 100101, China 2. International Institute for Applied System Analysis, A-2361 Laxenburg, Austria) | 2002 | Journal of Geographical Sciences2002，12，3： | 17 |
| 3 | Regional disparity in the changes of agricultural land use intensity in China during 1980-2002显示文摘Based on the cost-benefit data (1980–2002) of farm products and China Agriculture Yearbooks, this paper studies the regional disparity in the changes of the agricultural land use in China during the period 1980–2002 from three aspects such as the degree of intensity, the sown area and the abandoned farmland. The results show that: (1) The degree of intensity of land use in the western region during 1980–2000 has a strong uptrend, but in the eastern and central regions the degree of intensity descends obviously and has shown a continuous downtrend since 1997. (2) The total sown area shrinks notably in the eastern region, while it enlarges constantly in the western region. (3) The sown area in the eastern, central and western regions has gone through a similar cyclic process: down (1980–1985)–up (1985–1991)–down (1991–1994)–up (1994–1999)–down (1999–2002). However, there are obvious differences in amplitude variation and tendency among them. The sown area has shrunk in the eastern region and expanded in the central and western regions especially before 1999. (4) The most cases of abandoned farmland are reported in the central region, the second in the eastern region and the least in the western region. The abandonment phenomena chiefly occurred during 1992–1995 in the eastern region, and during 1998–2002 in the central region. | LIU Chengwu LI Xiubin | 2006 | Journal of Geographical Sciences2006，16，3： | 16 |
| 4 | Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells显示文摘Mechanical properties play an important role in regulating cellular activities and are critical for unlocking the mysteries of life. Atomic force microscopy (AFM) enables researchers to measure mechanical properties of single living cells under physiological conditions. Here, AFM was used to investigate the topography and mechanical properties of red blood cells (RBCs) and three types of aggressive cancer cells (Burkitt's lymphoma Raji, cutaneous lymphoma Hut, and chronic myeloid leukemia K562). The surface topography of the RBCs and the three cancer cells was mapped with a conventional AFM probe, while mechanical properties were investigated with a microsphere glued onto a tip-less cantilever. The diameters of RBCs are significantly smaller than those of the cancer cells, and mechanical measurements indicated that Young's modulus of RBCs is smaller than those of the cancer cells. Aggressive cancer cells have a lower Young's modulus than that of indolent cancer cells, which may improve our understanding of metastasis. | LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing | 2012 | Science China(Life Sciences)2012，55，11： | 16 |
| 5 | Hyperbaric oxygen therapy improves cognitive functioning after brain injury显示文摘Hyperbaric oxygen therapy has been widely applied and recognized in the treatment of brain injury;however,the correlation between the protective effect of hyperbaric oxygen therapy and changes of metabolites in the brain remains unclear.To investigate the effect and potential mechanism of hyperbaric oxygen therapy on cognitive functioning in rats,we established traumatic brain injury models using Feeney’s free falling method.We treated rat models with hyperbaric oxygen therapy at 0.2 MPa for 60 minutes per day.The Morris water maze test for spatial navigation showed that the average escape latency was significantly prolonged and cognitive function decreased in rats with brain injury.After treatment with hyperbaric oxygen therapy for 1 and 2 weeks,the rats’spatial learning and memory abilities were improved.Hydrogen proton magnetic resonance spectroscopy analysis showed that the N-acetylaspartate/creatine ratio in the hippocampal CA3 region was significantly increased at 1 week,and the N-acetylaspartate/choline ratio was significantly increased at2 weeks after hyperbaric oxygen therapy.Nissl staining and immunohistochemical staining showed that the number of nerve cells and Nissl bodies in the hippocampal CA3 region was significantly increased,and glial fibrillary acidic protein positive cells were decreased after a 2-week hyperbaric oxygen therapy treatment.Our findings indicate that hyperbaric oxygen therapy significantly improves cognitive functioning in rats with traumatic brain injury,and the potential mechanism is mediated by metabolic changes and nerve cell restoration in the hippocampal CA3 region. | Su Liu Guangyu Shen Shukun Deng Xiubin Wang Qinfeng Wu Aisong Guo | 2013 | Neural Regeneration Research2013，8，35： | 16 |
| 6 | Tillage Pedogenesis of Purple Soils in Southwestern China显示文摘Land cultivation and tillage process, and their consequent impacts on soil erosion, have been criticized as the main cause of degradation of land or soil quality. However, purple soils, classified as Regosols in FAO Taxonomy or Entisols in USDA Taxonomy, are formed from purple rocks of the Trias-Cretaceous system, have been developed or at least accelerated the development due to continual tillage operation, especially digging and ridging. The present study took micromorphological investigation on the sedimentary rocks and the soils under different operations of tillage. Results show that the purple rock of Feixiangguan Formation of the Trias system (T1f) is the easiest to physical weathering and the most fertile soil material enriched in nutrients, and it has been, therefore, mostly cultivated and intensively tilled around the year. It has the fastest soil formation rate. Soil formation rate in the cropland with conventional tillage is higher than that in the forestland and the grassland. It implies that the artificial brokenness and tillage disturbance play a great role in physical weathering and initiating soil formation processes. | HE Xiubin BAO Yuhai NAN Hongwei XIONG Donghong WANG Li LIU Yanfeng ZHAO Jingbo | 2009 | Journal of Mountain Science2009，6，2： | 10 |
| 7 | Deformation geometry and timing of the Wupoer thrust belt in the NE Pamir and its tectonic implications显示文摘 | Xiaogan CHENG Hanlin CHEN Xiubin LIN Shufeng YANG Shenqiang CHEN Fenfen ZHANG Kang LI Zelin LIU | 2016 | Frontiers of Earth Science2016，10，4： | 6 |
| 8 | Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy显示文摘Atomic force microscopy (AFM) was used to locate CD20 molecules on the surface of lymphoma Raji cells. Rituximab (a monoclonal antibody against CD20) molecules were linked onto the AFM tip via a polyethylene glycol (PEG) linker. Raji cells were adsorbed onto glass slides coated with poly-L-lysine. First, the CD20 distribution in a local area of the cell surface was visualized using the AFM lift scan mode. Second, 16 × 16 force curves were obtained from the same cell area to construct the CD20-rituximab binding force map. Finally, free rituximab was added to block the CD20 molecules on the cell surface and the lift phase image and CD20-rituximab force map were obtained again. The experimental results indicated that when the lift height was greater than the length of the PEG linker, no recognition sites were observed in the lift phase image. However, as the lift height decreased to the length of the PEG linker, some recognition sites were observed in the lift phase image and these sites were generally consistent with the pixels in the force map. After blocking, both the recognition sites in the lift phase image and the gray pixels in the binding force map decreased markedly. These results can improve our understanding of the distribution of protein molecules on the cell surface and facilitate further investigations into cellular functions. | LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing | 2013 | Chinese Science Bulletin2013，58，13： | 6 |
| 9 | Atomic force microscopy imaging of live mammalian cells显示文摘Atomic force microscopy (AFM) was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell retracted, many small sawtooth-like filopodia formed and reorganized, and the thickness of cellular lamellipodium increased as the retraction progressed. In elongated Neuro-2a cells, the cytoskeleton reorganized from an irregular to a parallel, linear morphology. Suspended mammalian cells were immobilized by method combining polydimethylsiloxane-fabricated wells with poly-L-lysine electrostatic adsorption. In this way, the morphology of a single live lymphoma cell was imaged by AFM. The experimental results can improve our understanding of cell locomotion and may lead to improved immobilization strategies. | LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing | 2013 | Science China(Life Sciences)2013，56，9： | 6 |
| 10 | The chromosome-level Hemerocallis citrina Borani genome provides new insights into the rutin biosynthesis and the lack of colchicine显示文摘Hemerocallis citrina Borani(huang hua cai in Chinese)is an important horticultural crop whose flower buds are widely consumed as a delicious vegetable in Asia.Here we assembled a high-quality reference genome of H.citrina using single-molecule sequencing and Hi-C technologies.The genome assembly was 3.77 Gb and consisted of 3183 contigs with a contig N50 of 2.09 Mb,which were further clustered into 11 pseudochromosomes.A larger portion(3.25 Gb or 86.20%)was annotated as a repetitive content and 54,295 protein-coding genes were annotated in the genome.Genome evolution analysis showed that H.citrina experienced a recent whole-genome duplication(WGD)event at~15.73 million years ago(Mya),which was the main factor leading to many multiple copies of orthologous genes.We used this reference genome to predict 20 genes involved in the rutin biosynthesis pathway.Moreover,our metabolomics data revealed neither colchicine nor its precursors in H.citrina,challenging the long-standing belief that this alkaloid causes poisoning by the plant.The results of our disruptive research are further substantiated by our genomic finding that H.citrina does not contain any genes involved in colchicine biosynthesis.The high-quality genome lays a solid foundation for genetic research and molecular breeding of H.citrina. | Zhixing Qing Jinghong Liu Xinxin Yi Xiubin Liu Guoan Hu Jia Lao Wei He Zihui Yang Xiaoyan Zou Mengshan Sun Peng Huang Jianguo Zeng | 2021 | Horticulture Research2021，8，1： | 6 |
| 11 | Progress of AFM single-cell and single-molecule morphology imaging显示文摘Atomic force microscopy (AFM) can probe single living cells and single native membrane proteins in natural fluid environments with label-free high spatial resolution. It has thus become an important tool for cellular and molecular biology that significantly complements traditional biochemical and biophysical techniques such as optical and electron microscopy and X-ray crystallog-raphy. Imaging surface topography is the primary application of AFM in the life sciences. Since the early 1990s, researchers have used AFM to investigate morphological features of living cells and native membrane proteins with impressive results. Steady improvements in AFM techniques for imaging soft biological samples have greatly expanded its applications. Based on the authors' own research in AFM imaging of living cell morphologies, a review of sample preparation procedures for single-cell and single-molecule imaging experiments is presented, along with a summary of recent progress in AFM imaging of living cells and native membrane proteins. Finally, the challenges of AFM high-resolution imaging at the single-cell and single-molecule levels are discussed. | LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing | 2013 | Chinese Science Bulletin2013，58，26： | 5 |
| 12 | Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy显示文摘Cell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy(AFM) was applied to quantitatively investigate the effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells. First, AFM indenting experiments were performed on six types of human cells to investigate the changes of cellular Young's modulus at different temperatures and the results showed that the mechanical responses to the changes of temperature were variable for different types of cancer cells. Second, AFM imaging experiments were performed to observe the morphological changes in living cells at different temperatures and the results showed the significant changes of cell morphology caused by the alterations of temperature. Finally, by co-culturing human cancer cells with human immune cells, the mechanical and morphological changes in cancer cells were investigated. The results showed that the co-culture of cancer cells and immune cells could cause the distinct mechanical changes in cancer cells, but no significant morphological differences were observed. The experimental results improved our understanding of the effects of temperature and cellular interactions on the mechanics and morphology of cancer cells. | LI Mi LIU LianQing XI Ning WANG YueChao XIAO XiuBin ZHANG WeiJing | 2015 | Science China(Life Sciences)2015，58，9： | 5 |
| 13 | Detecting CD20-Rituximab interaction forces using AFM single-molecule force spectroscopy显示文摘The invention of atomic force microscopy(AFM) has provided new technology for measuring specific molecular interaction forces.Using AFM single-molecule force spectroscopy(SMFS) techniques,CD20-Rimximab rupture forces were measured on purified CD20 proteins,Raji cells,and lymphoma patient B cells.Rimximab molecules were linked onto AFM tips using AFM probe functionalization technology,and purified CD20 proteins were attached to mica using substrate functionalization technology.Raji cells(a lymphoma cell line) or lymphoma patient cells were immobilized on a glass substrate via electrostatic adsorption and chemical fixation.The topography of the purified CD20 proteins,Raji cells,and patient lymphoma cells was visualized using AFM imaging and the differences in the rupture forces were analyzed and measured.The results showed that the rupture forces between the CD20 proteins on Raji cells and Rituximab were markedly smaller than those for purified CD20 proteins and CD20 proteins on lymphoma patient B cells.These findings provide an effective experimental method for investigating the mechanisms underlying the variable efficacy of Rituximab. | LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi LI GuangYong XIAO XiuBin ZHANG WeiJing | 2011 | Chinese Science Bulletin2011，56，35： | 3 |
| 14 | Luteolin prevents uric acid-induced pancreatic β-cell dysfunction显示文摘Elevated uric acid causes direct injury to pancreaticβ-cells.In this study,we examined the effects of luteolin,an important antioxidant,on uric acid-inducedβ-cell dysfunction.We first evaluated the effect of luteolin on nitric oxide(NO)formation in uric acid-stimulated Min6 cells using the Griess method.Next,we performed transient transfection and reporter assays to measure transcriptional activity of nuclear factor(NF)-κB.Western blotting assays were also performed to assess the effect of luteolin on the expression of MafA and inducible NO synthase(iNOS)in uric acid-treated cells.Finally,we evaluated the effect of luteolin on uric acid-induced inhibition of glucose-stimulated insulin secretion(GSIS)in Min6 cells and freshly isolated mouse pancreatic islets.We found that luteolin significantly inhibited uric acid-induced NO production,which was well correlated with reduced expression of iNOS mRNA and protein.Furthermore,decreased activity of NF-κB was implicated in inhibition by luteolin of increased iNOS expression induced by uric acid.Besides,luteolin significantly increased MafA expression in Min6cells exposed to uric acid,which was reversed by overexpression of iNOS.Moreover,luteolin prevented uric acidinduced inhibition of GSIS in both Min6 cells and mouse islets.In conclusion,luteolin protects pancreaticβ-cells from uric acid-induced dysfunction and may confer benefit on the protection of pancreaticβ-cells in hyperuricemiaassociated diabetes. | Ying Ding Xuhui Shi Xuanyu Shuai Yuemei Xu Yun Liu Xiubin Liang Dong Wei Dongming Su | 2014 | The Journal of Biomedical Research2014，28，4： | 3 |
| 15 | TLR4-mediated MyD88-dependent signaling pathway is activated by cerebral ischemia–reperfusion in cortex in mice显示文摘 | Yin Gao Xiubin Fang Yue Tong Yuli Liu Baohui Zhang | 2008 | Biomedicine & Pharmacotherapy2008，，6： | 1 |
| 16 | Building new countryside in China: A geographical perspective显示文摘 | Hualou Long Yansui Liu Xiubin Li Yufu Chen | 2009 | Land Use Policy2009，，2： | 1 |
| 17 | A new method for measuring firewall throughput显示文摘 | Liu Guowei Qian Xiubin Yan Tengfei Huang Shaoqing | | 0，，09： | 1 |
| 18 | Building new countryside in China:A geographical perspective显示文摘 | Long Hualou Liu Yansui Li Xiubin | | 0，，02： | 1 |
| 19 | Building new countryside in China: A geographical perspective显示文摘 | Long Hualou Liu Yansui Li Xiubin | 2010 | Land Use Policy2010，27，2： | 1 |
| 20 | Building new countryside in China: A geographical perspective 显示文摘 | Long Hualou Liu Yansui Li Xiubin Chen Yufu | 2010 | Land Use Policy2010，，2： | 1 |
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