|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface显示文摘A new cell membrane preparation, silica carrier cell membrane (CCM), has been presented, in which cell membrane was immobilized on silica which acted as carrier by the adsorption. The surface characteristics of the silica CCM have been tested by a scanning electron microscope and surface energy spectrometer. Comparison has been made of the changeable rule of enzymatic activity of rabbit red cell membrane in three states: the silica CCM, the suspension and the precipitation cell membranes, with temperature and time. The result showed that the silica CCM still keeps the same enzymatic activity as the other two states of cell membranes. The interactions between calcium antagonists and rabbit red and cardiac muscle cell membranes, and the stereoscopic interactions between dihydropridines and rabbit cerebellar cell membrane have been investigated by the chromatographic system of the silica CCM as a stationary phase. From the foregoing results, the chromatographic parameters obtained from the system would | Langchong He Guangde Yang Xindu Geng | 1999 | Chinese Science Bulletin1999，44，9： | 58 |
| 2 | Determination of free energy of protein folding on liquid-solid interface显示文摘Based on the fact that the stoichiometric displacement model for retention of solute and the total adsorption free energy of solute on a solid surface can be divided into two components, net adsorption and net desorbed energies, a new principle and an equation for calculating the free energy of protein folding, △△GF, on the solid surface are proposed. With high-performance hydrophobic interaction chromatography (HPHIC), an experimental method for determining the △△GF is established. Lysozyme and a-amylase have been selected as examples to test the new method, and their △△GF on the HPHIC stationary phase surface are found to be much higher than that reported from a solution. In addition, the △△GF of the two proteins are found to increase with the concentration of denaturing agent employed. The average standard deviations,±4.7% for lysozyme and ± 3.0% for a-amylase, indicate that the new method has a satisfactory reproducibility and reliability. | Xindu Geng Jing Zhang Yinmao Wei | 2000 | Chinese Science Bulletin2000，45，3： | 5 |
| 3 | A new chromatographic method for fast separation of active proteins显示文摘A new method with only a single chromatographic column to accomplish the separation of intact protein molecules by the combination of ion exchange chromatography and hydrophobic interaction chromatography is firstly presented. By selecting twice a suitable stationary phase, mobile phase, buffer exchange condition, and sample injection, some proteins usually required to separate with two-dimensional chromatography can now be accomplished only using the single column in one hour. In addition, the separated proteins can maintain their original three-or four-dimensional molecular structure. It would be expected that this method can also provide a new thought for sample pre-frac-tionation in proteomic investigation, especially, for the limitation of sample source. | KE CongYu GENG XinDu | 2008 | Chinese Science Bulletin2008，53，7： | 4 |
| 4 | Recent developments of the stoichiometric displacement model for separation processes显示文摘Substantial attention has been paid to the stoi-chiometric displacement models for solute retention (SDM-R) in liquid chromatography and adsorption (SDM-A) at surfaces since they were developed. The SDM has a strongly theoretical basis and has evolved to the point that it is widely applied in chemistry, biochemistry, molecular biology and biotechnology. This review introduces the history, recent developments, and new concepts relating to the SDM, including theory, mathematical expressions, and applications across a broad range of fields. | GENG Xindu Fred E.Regnier WANG Yan | 2001 | Chinese Science Bulletin2001，46，21： | 2 |
| 5 | Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics显示文摘 | Langchong He Sicen Wang Xindu Geng | 2001 | Chromatographia (-)2001，，1： | 1 |
| 6 | Retention model for proteins in reversed-phase liquid chromatography显示文摘 | Geng Xindu Regnier FE | 1984 | J Chromatogr1984，296，： | 1 |
| 7 | Stoichiometric displacement of solvent by nonpolar solutes in reversed-phase liquid chromatography显示文摘 | Geng Xindu Regnier FE | 1985 | J Chromatogr1985，332，： | 1 |
| 8 | Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics显示文摘 | Langchong He Sicen Wang Xindu Geng | 2001 | Chromatographia (-)2001，，1： | 1 |
| 9 | Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics显示文摘 | Langchong He Sicen Wang Xindu Geng | 2001 | Chromatographia (-)2001，，1： | 1 |
| 10 | Coating and Fusing Cell Membrane Onto a Silica Surface and Their Chromatographic Characteristics显示文摘 | He Langchong Wang Sicen Geng Xindu | 2001 | Chromatographia2001，54，： | 1 |
| 11 | Coating and Fusing Cell Membrane Onto a Silica Surface and Their Chromatographic Characteristics 显示文摘 | Sicen Wang Xindu Geng | 2001 | Chromatographia2001，54，： | 1 |
| 12 | Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics显示文摘 | Langchong He Sicen Wang Xindu Geng | 2001 | Chromatographia (-)2001，，1： | 1 |
| 13 | Influences of the mobile phase composition and temperature on the retention behavior of aromatic alcohol homologues in hydrophobic interaction chromatography显示文摘 | Yinmao Wei Cong Yao Jianguo Zhao Xindu Geng | 2002 | Chromatographia (-)2002，，11： | 1 |
| 14 | Fast separation of intact proteins with high resolution under high sample loading using non-porous RPLC packings显示文摘A new approach for separation of intact proteins with high resolution, high speed and high sample loading ('three highs') by non-porous packings in high performance liquid chromatography is presented.A chromatographic cake having larger diameter than its thickness is firstly employed to completely separate 1.0 and 40 μg of the mixture of 7 standard proteins within 1 min un-der conventional chromatographic conditions.Also, 0.5 mg of the mixture was almost completely separated within 1 min, accom-plishing the 'three high' purpose.This research expands the application utilized for separation of intact proteins with non-porous packings.A smaller geometric size of chromatographic cake packed with much smaller particles of non-porous packings may improve results. | WANG Fei GENG XinDu | 2010 | Chinese Science Bulletin2010，55，16： | 0 |
| 15 | Effect of ligand structure of stationary phase of high performance hydrophobic interaction chromatography on renaturation efficiency of GuHCl-denaturedα-chymotrypsin显示文摘The renaturation of the denatured α-chymotrypsin (α-Chy) with 1.7 mol · L-1 guanidine hydrochloride (GuHCI) by three kinds of stationary phase of high performance hydrophobic interaction chromatography (STHIC) with a comparable hydrophobicity but different ligand structures was investigated. The obtained result indicates that the ligand structures of the three STHIC contribute to the renaturation efficiency of α-Chy in the order of the end ligands PEG-600< phenyl group < tetrahydrofurfuryl alcohol (THFA). | SHEN Yehua WANG Haibo BAI Quan GENG Xindu | 2005 | Science China Chemistry2005，48，z1： | 0 |
| 16 | Studies on the refolding of the reduced-denatured insulin with size exclusion chromatography显示文摘The refolding of the reduced-denatured insulin from bovine pancreas was investigated with the size exclusion chromatography (SEC). It was shown that the reduced-denatured insulin originally denatured with 7.0 mol·L-1 guanidine hydrochloride (GuHCl) or 8.0 mol·L-1 urea could not be refolded with a non-oxidized mobile phase. Although the oxidized and reduced glutathione (GSSG and GSH) were employed in the oxidized mobile phase, the reduced-denatured insulin still could not be renatured. However, in the presence of 2.0 mol·L-1 urea in the oxidized mobile phase employed, the reduced-denatured insulin can be refolded with SEC, and the aggregation of denatured insulin can be diminished by urea. In addition, the disulfide exchange of reduced-denatured insulin also can be accelerated with GSSG/GSH in the oxidized mobile phase. The three disulfide bridges of insulin were formed correctly and the reduced-unfolded insulin can be renatured completely. The results were further tested with reversed-phase liquid chromatography (RPLC) and hydrophobic interaction chromatography (HIC). | BAI Quan KONG Yu DONG Cuihua GENG Xindu | 2005 | Science China Chemistry2005，48，z1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员