|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Association of H.pylori infection with gastric carcinoma:a Meta analysis显示文摘AIM: To follow the principles of evidence based medicine to reach the integrated results of these studies.METHODS: Twenty-one papers of case-control studies were selected, including 11 on gastric cancer, 7 on precancerous lesion of stomach and 3 on lymphoma of stomach: Meta analysis was used to sum up the odds ratios (OR) of these studies.RESULTS: H. Pylori vsgastric cancer (intestinal and diffuse type): the odds ratio from the fixed effect model is 3.0016(95% Cl 2.4197-3.7234, P < 0.001 ). H. Pylori vs precancerous lesion of stomach: a random effect model was used to calculate the summary odds ratio and its value is 2.5635 (95% Cl: 1.8477-3.5566, P < 0.01). H. Pylori vs lymphoma of stomach: though the quantity of literature is too small to make Meta analysis, the data of these 3 studies show that lymphoma of stomach is highly associated with H. Pylori infections.CONCLUSION: Since it had been revealed that H. Pylori infection pre-exists in gastric carcinoma and precancerous lesions, the results of Meta analysis present a strong evidence to support the conclusion that H. Pylori infection is a risk factor for gastric carcinoma. | Fu-Bo Xue~1 Yong-Yong Xu~1 Yi Wan~1 Bo-Rong Pan~2 Jun Ren~2 Dai-Ming Fan~3 1 Department of Health Statistics,Department of2 Oncology3 Gastroenterology of XiJing Hospital,the Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，6： | 66 |
| 2 | Effect of notoginsenoside R1 on hepatic microcirculation disturbance induced by gut ischemia and reperfusion显示文摘AIM: To assess the effect of notoginsenoside R1 on hepatic microcirculatory disturbance induced by gut ischemia/reperfusion (I/R) in mice.METHODS: The superior mesenteric artery (SMA) of C57/BL mice was ligated for 15 min to induce gut ischemia followed by 30-min reperfusion. In another set of experiments, R1 was continuously infused (10 mg/kg per hour) from 10 min before I/R until the end of the investigation to study the influence of R1 on hepatic microcirculatory disturbance induced by gut I/R. Hepatic microcirculation was observed by inverted microscopy, and the vascular diameter, red blood cell (RBC) velocity and sinusoid perfusion were estimated. Leukocyte rolling and adhesion were observed under a laser confocal microscope. Thirty and 60 min after reperfusion, lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate transaminase (AST) in peripheral blood were determined. The expression of adhesion molecules CD11b/CD18 in neutrophils and tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in plasma were evaluated by flow cytometry. E-selectin and intercellular adhesion molecule-1 (ICAM-1) in hepatic tissue were examined by immunofluorescence. RESULTS: After gut I/R, the diameters of terminal portal venules and central veins, RBC velocity and the number of perfused sinusoids were decreased, while the leukocyte rolling and adhesion, the expression of E-selectin in hepatic vessels and CD18 in neutrophils, IL-6, MCP-1, LDH, ALT and AST were increased. R1 treatment attenuated these alterations except for IL-6 and MCP-1.CONCLUSION: R1 prevents I/R-induced hepatic microcirculation disturbance and hepatocyte injury. The effect of R1 is related to its inhibition of leukocyte rolling and adhesion by inhibiting the expression of E-selectin in endothelium and CD18 in neutrophils. | Wei-Xing Chen Fang Wang, Yu-Ying Liu, Qing-Jiang Zeng, Kai Sun, Xin Xue, Xiang Li, Ji-Ying Yang, Li-Hua An,Bai-He Hu, Jin-Hui Yang, Chuan-She Wang, Zhi-Xin Li, Lian-Yi Liu, Yan Li, Jun Zheng, Fu-Long Liao, Dong Han,Jing-Yu Fan, Jing-Yan Han Fang Wang Yu-Ying Liu Qing-Jiang Zeng Kai Sun Xin Xue Xiang Li Ji-Ying Yang Li-Hua An Bai-He Hu Jin-Hui Yang Chuan-She Wang Zhi-Xin Li Lian-Yi Liu Yan Li Jun Zheng Fu-Long Liao Dong Han Jing-Yu Fan Jing-Yan Han | 2008 | World Journal of Gastroenterology2008，14，1： | 17 |
| 3 | Perioperative transcutaneous electrical acupoint stimulation for improving postoperative gastrointestinal function:A randomized controlled trial显示文摘Background:Postoperative gastrointestinal dysfunction(PGD)is one of the most common complications in patients undergoing major abdominal surgery.Acupuncture has been used widely in gastrointestinal diseases due to its effectiveness and minimally invasive nature.Objective:This study evaluated the efficacy of using transcutaneous electrical acupoint stimulation(TEAS)during the surgery and postoperative recovery in patients with gastric and colorectal surgery for improving postoperative gastrointestinal function.Design,setting,participants and interventions:A total of 280 patients undergoing abdominal surgery were stratified by type of surgery(i.e.,gastric or colorectal surgery)and randomly allocated into the TEAS group(group T)or the sham group(group S).Patients in group T received TEAS at LI4,PC6,ST36 and ST37.Patients in group S received pseudo-TEAS at sham acupoints.The stimulation was given from 30 min before anesthesia until the end of surgery.The same treatment was performed at 9 am on the 1st,2nd and 3rd days after surgery,until the recovery of flatus in patients.Main outcome measures:The primary outcome was the time to the first bowel motion,as detected by auscultation.The secondary outcomes included the first flatus and ambulation time,changes of perioperative substance P(SP),incidence of PGD,postoperative pain,postoperative nausea and vomiting(PONV)and some economic indicators.Results:The time to first bowel motion,first flatus and first ambulation in group T was much shorter than that in group S(P<0.01).In patients undergoing colorectal surgery,the concentration of SP was lower in group T than in group S on the third day after the operation(P<0.05).The average incidence of PGD in all patients was 25%,and the frequency of PGD was significantly lower in group T than in group S(18.6%vs.31.4%,respectively;P<0.05).TEAS treatment(odds ratio=0.498;95%confidence interval:0.232–0.786)and type of surgery were relevant factors for the development of PGD.Postoperative pain score and PONV occurrence were significantly lower in group T(P<0.01).Postoperative hospitalization days and the resulting cost to patients were greatly reduced in the TEAS group(P<0.01).Conclusion:Perioperative TEAS was able to promote the recovery of postoperative gastrointestinal function,reduce the incidence of PGD and PONV.The concentration of SP was decreased by TEAS treatment,which indicates that the brain-gut axis may play a role in how TEAS regulates gastrointestinal function.Trial registration:Chinese Clinical Trial Registry,Chi CTR1900023263. | Wen-jing Li Chao Gao Li-xin An Yu-wei Ji Fu-shan Xue Yi Du | 2021 | Journal of Integrative Medicine2021，19，3： | 16 |
| 4 | Modern pollen rain in the Lake Qinghai basin, China显示文摘Lake Qinghai is the largest inland brackish lake in China and lies within the NE Tibetan Plateau. Our study shows that pollen assemblages in each vegetation belt are significantly correlated with the vegetation types of this area. Among the herbaceous and shrubby pollen assemblages, Artemisia is over-represented, while Poaceae, Cyperaceae and Polygonaceae are under-represented. Artemisia/ Chenopodiaceae (A/C) ratios with the regional vegetation characteristic can be used as a proper index to reconstruct the history of vegetation and climate in Lake Qinghai basin. Modern pollen in the lake mainly comes from the nearby vegetation, controlled by the directions and velocity of the wind. The distribution of modern pollen in Lake Qinghai tends to be similar in most part of the lake. The difference of pollen sedimentation process in the lake can be potentially influenced by the focusing function of the lake, river streams, and lake current. | SHANG Xue LI XiaoQiang AN ZhiSheng JI Ming ZHANG HongBin | 2009 | Science China Earth Sciences2009，52，10： | 15 |
| 5 | Paleoenvironments indicated by the fossil mammalian assemblages from red clay-loess sequence in the Chinese Loess Plateau since 8.0 Ma B.P.显示文摘Information from eleven profiles of eolian earthy red silty clay and loess of the middle of the Late Miocene to Holocene age have been studied and correlated. A complete summary profile with projected isotopic ages and fossil-bearing beds has been assembled. The profile is subdivided into 12 stratigraphic units proceeding from the lower (older) to the upper (younger) one. The character of mammalian assemblages contained in each unit was analyzed and the corresponding pa- leo-climatic environments were deduced. The environmental character of each period and the change from the middle of the Late Miocene at about 8.0 Ma B.P. up to the Holocene progressed from the hot semiarid and semi-moist, warmer-moist, warm-moister of the Late Miocene. To slightly less warm moist, cool-dry, slight-warmer-moister of the Pliocene; later the cool to cold dry periods alternated frequently with the mild semiarid and semi-moist periods of the Quaternary. Vegetation progressed from the grasslands with sparse woods, wooded shrub-grasslands and subtropical forest-grasslands of the Late Miocene age to sparse grasslands and dry grasslands of the Pliocene; and to sparse grasslands, dry grasslands and tundras of the Quaternary. The climate changes in the Neogene were of low-amplitute in a generally warm-humid background. Those of the Quaternary were of higher am- plitude with a longer phase. Cool or cold dry and slightly warm semiarid and semi-moist climates al- ternated on a generally cool-dry background. The tendency since the start of the Quaternary was the change to more northern and western shift in the Loess Plateau, with progressively cooler and dryer conditions. The dramatic uplift of the Qinghai-Xizang (Tibet) Plateau and the forming and expansion of the Arctic ice sheet might be the main reasons of the more pronounced changes in the Quaternary. | XUE Xiangxu1,2, ZHANG Yunxiang1,2 & YUE Leping1,2 1. State Key Laboratory of Continent Dynamics (Northwest University), Institute of Cenozoic Geology and Environment, De- partment of Geology, Northwest University, Xi’an 710069, China 2. Institute of Earth Environment, State Key Laboratory of Loess and Quaternary Geology, Chinese Academy of Sciences, Xi’an 710075, China | 2006 | Science China Earth Sciences2006，49，5： | 13 |
| 6 | Acupuncture therapy on apoplectic aphasia rehabilitation显示文摘OBJECTIVE:Acupuncture has often been used for aphasia rehabilitation in China.The purpose of this paper was to:1) provide a historic overview of acupuncture for aphasia due to stroke;2) summarize the commonly used acupuncture approaches;and 3) objectively comment on the effectiveness of acupuncture for the rehabilitation of this type of disorder.METHODS:The Elsevier database and a Chinese database(CNKI) were searched through December,2010 with the key words 'aphasia,acupuncture' in English and Chinese,respectively.Case reports,uncontrolled clinical observations and controlled clinical trials were all included if acupuncture was the sole treatment or the main component of complex intervention for the rehabilitation of aphasia caused by cerebrovascular disease.RESULTS:More than 100 relevant articles were found.After analyzing these articles,we found that acupuncture for apoplectic aphasia most often included tongue,scalp,body and combination acupuncture.Tongue bleeding,deep insertion and strong stimulation were adopted by many practitioners.The ten most frequently used acupoints(or areas) were Lianquan(RN 23),Jinjin(EX-HN 12),Yuye(EX-HN 13),Tongli(HT 5),Fengchi(GB 20),Neiguan(PC 6),Baihui(DU 20),No.1,2 and 3 language sections,Sanyinjiao(SP 6) and Yamen(DU 15).CONCLUSIONS:Controlled clinical studies and a systematic literature review demonstrate that acupuncture has therapeutic effects on aphasia after stroke. | Youzhi Sun Steve An Xue Zhengyun Zuo | 2012 | Journal of Traditional Chinese Medicine2012，32，3： | 13 |
| 7 | Anti-HBV effect of TAT-HBV targeted ribonuclease显示文摘AIM: To prepare and purify TAT-HBV targeted ribonuclease fusion protein, evaluate its transduction activity and investigate its effect on HBV replication in 2.2.15 cells.METHODS: The prokaryotic expression vector pTAT containing TR gene was used in transforming E. coli BL21(DE3) LysS and TR was expressed with the induction of IPTG. The TAT-TR fusion protein was purified using Ni-NTA-agrose and PD-10 desalting columns, and analyzed by SDSPAGE. Transduction efficiency of TAT-TR was detected with immunofluorescence assay and the concentration of HBeAg in the supernatant of the 2.2.15 cells was determined via solid-phase radioimmunoassay (spRIA). MTT assay was used to detect the cytotoxicity of TAT-TR.RESULTS: The SDS-PAGE showed that the TAT-TR fusion protein was purified successfully, and the purity of TAT-TR was 90 %. The visualization of TAT-TR by immunofiuorescence assay indicated its high efficiency in transducing 2.2.15 cells.RIA result suggests that TAT-TR could inhibit the replication of HBV effectively, it didn′t affect cell growth and had no cytotoxicity.CONCLUSION: TAT-TR possesses a significant anti-HBV activity and the preparation of TAT-TR fusion protein has laid the foundation for the use of TR in the therapeutic trial of HBV infection. | Jin Ding Jun Liu Cai-Fang Xue Wei-Dong Gong Ying-Hui Li Ya Zhao Department of Etiology,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2003 | World Journal of Gastroenterology2003，9，7： | 12 |
| 8 | A versatile cooperative template-directed coating method to synthesize hollow and yolk-shell mesoporous zirconium titanium oxide nanospheres as catalytic reactors显示文摘 | Buyuan Guan Tao Wang Shangjing Zeng Xue Wang Dong An Dongmei Wang Yu Cao Dingxuan Ma Yunling Liu Qisheng Huo | 2014 | Nano Research2014，7，2： | 12 |
| 9 | Pb isotope geochemistry of lead, zinc, gold and silver deposit clustered region, Liaodong rift zone,northeastern China显示文摘Pb isotopic analyses were reported for sulfide and hydrothermal carbonate minerals and marble of the Xiquegou lead-zinc, the Zhenzigou zinc-lead and the Gaojiapuzi silver deposits from the Qingchengzi ore field and the Beiwagou zinc-lead deposit in the west, Proterozoic Liaodong rift zone. Pb isotopic ratios of the marble from the Qingchengzi ore field range from 18.24 to 30.63 for 206 Pb/204Pb, 15.59 to 17.05 for207Pb/204Pb and 37.43 to 38.63 for 208Pb/204Pb. The marble gives a Pb-Pb isochron age of 1822±92 Ma, which is interpreted as the age of the metamorphism of the marble. Ore Pb, including Pb of sulfide and hydrothermal car- bonate minerals, from the Qingchengzi ore field shows limited variation with 206 Pb/204Pb=17.66- 17.96, 207 Pb/204Pb=15.60-15.74 and 208Pb/204Pb=37.94-38.60. In contrast, ore Pb from the Beiwagou deposit gives different Pb isotopic ratios with 206Pb/204Pb=15.68-15.81, 207 Pb/204Pb= 15.34-15.45 and 208Pb/204Pb=35.30-35.68. Pb of all deposits from the Liaodong rift zone is derived from the upper crust. Ore Pb of the Qingchengzi deposits is derived from a young upper crust. The model Th/U ratios of 4.40 to 4.74 for ore Pb are significantly different from that of 1.7 to 4.4 given by the marble of the Qingchengzi ore field, suggesting that marble is not the source of the ore Pb. Ore Pb of the Beiwagou deposit is extracted from its source and the deposit is formed at the Paleoproterozoic era. Different Pb isotopic ratios of the Qingchengzi ore field and the Beiwagou deposit are due to different ages of the deposits and suggest that the two types of deposits are derived from different sources and are possibly formed by different ore-forming processes. | CHEN Jiangfeng1, YU Gang1, XUE Chunji2, QIAN Hui1, HE Jianfeng1, XING Zhi1 & ZHANG Xun1 1. School of Earth and Space Sciences, University of Science and Technology of China, Hefei 230026, China 2. Department of Geology, Chang’an University, Xi’an 710054, China | 2005 | Science China Earth Sciences2005，48，4： | 11 |
| 10 | Characteristics and formation mechanism of regional haze episodes in the Pearl River Delta of China显示文摘To investigate the characteristics and the specific mechanism of continuous haze,comprehensive measurements were conducted from 15 October to 19 November in the Atmospheric Environment Monitoring Super-Station in Heshan of Guangdong province.Five haze episodes occurred in October and November 2014 in the Pearl River Delta(PRD)region. The meteorological parameters, gas data, chemical compositions, and optical parameters of the aerosols were obtained. Among these events, the second haze episode,with the highest concentration of PM2.5 of 187.51 μg/m^3, was the most severe. NO^3-was always higher than SO_4^(2-), which indicated that motor vehicles played an important role in the haze, even though the oxidation rate from SO_2 to SO_4^(2-)was faster than that of NOXto NO_3^-. The difference between the hourly averages of Na+and K+during the haze episode and clean days was small, implying that straw combustion and sea salt had no significant effect on the occurrence of haze, and the backward trajectories of the air masses also conformed with this result. The air pollutants were difficult to disperse because of the significant decrease in the planetary boundary layer(PBL) height. Relative humidity played a crucial role in the formation of haze by leading to hygroscopic growth of the diameter of aerosols. | Wenguang Li Xingang Liu Yuanhang Zhang Kang Sun Yusheng Wu Rui Xue Limin Zeng Yu Qu Junling An | 2018 | Journal of Environmental Sciences2018，30，1： | 11 |
| 11 | Treatment of nutcracker syndrome with spermatic vein ligation and iliac vein anastomosis:Case report of three cases显示文摘Three patients with nutcracker syndrome(NCS) were enrolled in the study.Their clinical symptoms mainly included proteinuria and serious varicocele.Color Doppler ultrasound and computed tomography showed that the patients had constricted abdominal aortas,reduced superior mesenteric artery angles,and thinned left renal veins.Patients then underwent left spermatic vein ligation and iliac vein anastomosis.They were checked tliree months after their operation,and results showed that their sperms were improved and their proteinuria disappeared. The color Doppler ultrasound showed new cycle pathways.NCS clinical manifestations are complicated,and need combination diagnosis of symptoms and auxiliary examination to detennine surgical indications.Ligation of spermatic vein and vein anastomosis can be used to treat NCS. | Xiao-Yong Gong Wei Zheng Hong Du Yi Lei Ya-Hui Xue Chao-Hui Xue Xian-Chao An Gang Zheng | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，11： | 10 |
| 12 | Cutting-balloon angioplasty before drug-eluting stent implantation for the treatment of severely calcified coronary lesions显示文摘BackgroundSeverely 钙化的冠的损害对汽球 angioplasty 糟糕作出回应,导致不完全、不均匀的 stent 扩大。因此,在 drug-eluting stent (DES ) 以前的足够的匾修正培植是为钙化的损害治疗的钥匙。这研究是评估为严重地钙化的冠的 lesions.MethodsNinety 切汽球 angioplasty 的安全和功效 -- 有严重地钙化的损害的二个连续病人(定义为钙弧 ≥180 °钙长度比率 ≥0.5 ) 在 DES 培植随机基于汽球类型被划分成二个组以前,与汽球膨胀对待:在常规汽球 angioplasty (BA ) 的 45 个病人组织,在切的汽球 angioplasty (CB ) 的 47 个病人组织。在 BA 组的七个盒子令人满意地没完成膨胀并且被变成 CB 组。 Intravascular 超声( IVUS )在汽球膨胀前并且在 stent 培植以后被执行获得质、量的损害特征并且评估 stent ,包括最小的腔代表性的区域( CSA ),钙化的弧和长度,最小的 stent CSA , stent 并置, stent 对称, stent 扩大,容器解剖,并且分叉容器监狱。在里面医院, 1 月、 6 月的主要不利心脏的事件(向) 是 reported.ResultsThere 不是在在二个组之间的临床的特征的统计差别,包括钙弧(222.2 °±22.2 °对 235.0 °±22.1 °, P = 0.570 ) ,钙长度比率(0.67 ±0.06 对 0.77 ±0.05, P = 0.130 ) ,并且在一种总线标准前的最小的腔 CSA (2.59 ±0.08 公里 2 对 2.52 ±0.08 公里 2, P = 0.550 ) 。在 stent 培植以后,最后的最小的 stent CSA (6.26 ±0.40 公里 2 对 5.03 ±0.33 公里 2 ;P = 0.031 ) 并且尖锐的腔获得(3.74 ±0.38 公里 2 对 2.44 ±0.29 公里 2, P = 0.015 ) 比 BA 组的在 CB 组是显著地更大的。没有统计上在在二个组之间的 stent 扩大, stent 对称,不完全的 stent 并置,容器解剖和分支容器监狱的差别。在在严重地钙化的损害的 DES 培植看起来是包括显著地更大的最后的 stent CSA 和更大的尖锐的腔获得的更多的功效以前, 30 天、 6 月的向率也不是 different.ConclusionsCutting 汽球 angioplasty,没有增加在操作和 MACE 率在期间的复杂并发症 6 月。 | Zhe TANG Jing BAI Shao-Ping SU Yu WANG Mo-Han LIU Qi-Cai BAI Jin-Wen TIAN Qiao XUE Lei GAO Chun-Xiu AN Xiao-Juan LIU | 2014 | Journal of Geriatric Cardiology2014，11，1： | 9 |
| 13 | Normal Structure and Function of Endothecium Chloroplasts Maintained by ZmMs33-Mediated Lipid Biosynthesis in Tapetal Cells Are Critical for Anther Development in Maize显示文摘Genic male sterility(GMS)is critical for heterosis utilization and hybrid seed production.Although GMS mutants and genes have been studied extensively in plants,it has remained unclear whether chloroplast-associated photosynthetic and metabolic activities are involved in the regulation of anther development.In this study,we characterized the function of ZmMs33/ZmGPAT6,which encodes a member of the glycerol-3-phosphate acyltransferase(GPAT)family that catalyzes the first step of the glycerolipid synthetic pathway.We found that normal structure and function of endothecium(En)chloroplasts maintained by ZmMs33-mediated lipid biosynthesis in tapetal cells are crucial for maize anther development.ZmMs33 is expressed mainly in the tapetum at early anther developmental stages and critical for cell proliferation and expansion at late stages.Chloroplasts in En cells of wild-type anthers function as starch storage sites before stage 10 but as photosynthetic factories since stage 10 to enable starch metabolism and carbohydrate supply.Loss of ZmMs33 function inhibits the biosynthesis of glycolipids and phospholipids,which are major components of En chloroplast membranes,and disrupts the development and function of En chloroplasts,resulting in the formation of abnormal En chloroplasts containing numerous starch granules.Further analyses reveal that starch synthesis during the day and starch degradation at night are greatly suppressed in the mutant anthers,leading to carbon starvation and low energy status,as evidenced by low trehalose-6-phosphate content and a reduced ATP/AMP ratio.The energy sensor and inducer of autophagy,SnRK1,was activated to induce early and excessive autophagy,premature PCD,and metabolic reprogramming in tapetal cells,finally arresting the elongation and development of mutant anthers.Taken together,our results not only show that ZmMs33 is required for normal structure and function of En chloroplasts but also reveal that starch metabolism and photosynthetic activities of En chloroplasts at different developmental stages are essential for normal anther development.These findings provide novel insights for understanding how lipid biosynthesis in the tapetum,the structure and function of En chloroplasts,and energy and substance metabolism are coordinated to maintain maize anther development. | Taotao Zhu Ziwen Li Xueli An Yan Long Xiaofeng Xue Ke Xie Biao Ma Danfeng Zhang Yijian Guan Canfang Niu Zhenying Dong Quancan Hou Lina Zhao Suowei Wu Jinping Li Weiwei Jin Xiangyuan Wan | 2020 | Molecular Plant2020，13，11： | 9 |
| 14 | Whole genome sequencing of 10K patients with acute ischaemic stroke or transient ischaemic attack: design, methods and baseline patient characteristics显示文摘Background and purpose Stroke is the second leading cause of death worldwide and the leading cause of mortality and long-term disability in China,but its underlying risk genes and pathways are far from being comprehensively understood.We here describe the design and methods of whole genome sequencing(WGS)for 10914 patients with acute ischaemic stroke or transient ischaemic attack from the Third China National Stroke Registry(CNSR-III).Methods Baseline clinical characteristics of the included patients in this study were reported.DNA was extracted from white blood cells of participants.Libraries are constructed using qualified DNA,and WGS is conducted on BGISEQ-500 platform.The average depth is intended to be greater than 30×for each subject.Afterwards,Sentieon software is applied to process the sequencing data under the Genome Analysis Toolkit best practice guidance to call genotypes of single nucleotide variants(SNVs)and insertion-deletions.For each included subject,21 fingerprint SNVs are genotyped by MassARRAY assays to verify that DNA sample and sequencing data originate from the same individual.The copy number variations and structural variations are also called for each patient.All of the genetic variants are annotated and predicted by bioinformatics software or by reviewing public databases.Results The average age of the included 10914 patients was 62.2±11.3 years,and 31.4%patients were women.Most of the baseline clinical characteristics of the 10914 and the excluded patients were balanced.Conclusions The WGS data together with abundant clinical and imaging data of CNSR-III could provide opportunity to elucidate the molecular mechanisms and discover novel therapeutic targets for stroke. | Si Cheng Zhe Xu Yang Liu Jinxi Lin Yong Jiang Yilong Wang Xia Meng Anxin Wang Xinying Huang Zhimin Wang Guohua Chen Songdi Wu Zhengchang Jia Yongming Chen Xuerong Qiu Jun Wu Binbin Song Weizhong Ji Zhongping An Wenjun Xue Lili Zhao Yu Geng Hongyan Li Hao Li Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，2： | 8 |
| 15 | Differential gene expression profiling of gastric intraepithelial neoplasia and early-stage adenocarcinoma显示文摘AIM:To investigate the differentiated whole genome expression profiling of gastric high-and low-grade intraepithelial neoplasia and early-stage adenocarcinoma.METHODS:Gastric specimens from an upper magnifying chromoendoscopic targeted biopsy were collected from March 2010 to May 2013.Whole genome expression profiling was performed on 19 low-grade intraepithelial neoplasia(LGIN),20 high-grade intraepithelial neoplasia(HGIN),19 early-stage adenocarcinoma(EGC),and 19 chronic gastritis tissue samples using Agilent 4×44K Whole Human Genome microarrays.Differentially expressed genes between different types of lesions were identified using an unpaired t-test and corrected with the Benjamini and Hochberg false discovery rate algorithm.A gene ontology(GO)enrichment analysis was performed using the Gene Spring software GX 12.6.The differentially expressed gene was verified using a real-time TaqManPCR assay with independent tissue samples,including 26 LGIN,15 HGIN,14 EGC,and 20 chronic gastritis.The expression of G0S2 were further validated by immunohistochemical staining(IHC)in 24 LGIN,40 HGIN,30 EGC and 61 chronic gastritis specimens.RESULTS:The gene expression patterns of LGIN and HGIN tissues were distinct.There were 2521 significantly differentially expressed transcripts in HGIN,with951 upregulated and 1570 downregulated.A GO enrichment analysis demonstrated that the most striking overexpressed transcripts in HGIN compared with LGIN were in the category of metabolism,defense response,and nuclear factorκB(NF-κB)cascade.While the vast majority of transcripts had barely altered expression in HGIN and EGC tissues,only 38 transcripts were upregulated in EGC.A GO enrichment analysis revealed that the alterations of the immune response were most prominent in the progression from HGIN to EGC.It is worth noting that,compared with LGIN,289 transcriptswere expressed at higher levels both in HGIN and EGC.A characteristic gene,G0/G1 switch 2(G0S2)was one of the 289 transcripts and related to metabolism,the immune response,and the NF-κB cascade,and its expression was validated in independent samples through real-time TaqManPCR and immunohistochemical staining.In real-time PCR analysis,the expression of G0S2 was elevated both in HGIN and EGC compared with that in LGIN(P<0.01 and P<0.001,respectively).In IHC analysis,G0S2 immunoreactivity was detected in the cytoplasmic of neoplastic cells,but was undetectable in chronic gastritis cells.The G0S2 expression in HGIN was higher than that of LGIN(P=0.012,χ2=6.28)and EGC(P=0.008,χ2=6.94).CONCLUSION:A clear biological distinction between gastric high-and low-grade intraepithelial neoplasia was identified,and provides molecular evidence for clinical application. | Xue Xu Lin Feng Yu Liu Wei-Xun Zhou Ying-Cai Ma Gui-Jun Fei Ning An Yuan Li Xi Wu Fang Yao Shu-Jun Cheng Xing-Hua Lu | 2014 | World Journal of Gastroenterology2014，20，47： | 8 |
| 16 | High-entropy(La_(0.2)Nd_(0.2)Sm_(0.2)Eu_(0.2)Gd_(0.2))_(2)Ce_(2)O_(7):A potential thermal barrier material with improved thermo-physical properties显示文摘High-entropy oxides(HEOs)are widely researched as potential materials for thermal barrier coatings(TBCs).However,the relatively low thermal expansion coefficient(TEC)of those materials severely restricts their practical application.In order to improve the poor thermal expansion property and further reduce the thermal conductivity,high-entropy(La_(0.2)Nd_(0.2)Sm_(0.2)Eu_(0.2)Gd_(0.2))_(2)Ce_(2)O_(7) is designed and synthesized in this work.The as-prepared multicomponent material is formed in a simple disordered fluorite structure due to the high-entropy stabilization effect.Notably,it exhibits a much higher TEC of approximately 12.0×10^(−6) K^(−1) compared with those of other high-entropy oxides reported in the field of TBCs.Besides,it presents prominent thermal insulation behavior with a low intrinsic thermal conductivity of 0.92 W·m^(−1)·K^(−1) at 1400℃,which can be explained by the existence of high concentration oxygen vacancies and highly disordered arrangement of multicomponent cations in the unique high-entropy configuration.Through high-temperature in-situ X-ray diffraction(XRD)measurement,this material shows excellent phase stability up to 1400℃.Benefiting from the solid solution strengthening effect,it shows a higher hardness of 8.72 GPa than the corresponding single component compounds.The superior thermo-physical performance above enables(La_(0.2)Nd_(0.2)Sm_(0.2)Eu_(0.2)Gd_(0.2))_(2)Ce_(2)O_(7) a promising TBC material. | Yun XUE Xiaoqin ZHAO Yulong AN Yijing WANG Meizhen GAO Huidi ZHOU Jianmin CHEN | 2022 | Journal of Advanced Ceramics2022，11，4： | 7 |
| 17 | Inhibition of HBV targeted ribonuclease enhanced by introduction of linker显示文摘AIM: To construct human eosinophil-derived neurotoxin (hEDN) and HBV core protein (HBVc) eukaryotic fusion expression vector with a linker (Gly4 Ser)3 between them to optimize the molecule folding, which will be used to inhibit HBV replication in vitro.METHODS: Previously constructed pcDNA3. 1(-)/TR was used as a template. Linker sequence was synthesized and annealed to form dslinker, and cloned into pcDNA3.1(-)/TR to produce plasmid pcDNA3.1(-)/HBc-linker. Then the hEDN fragment was PCR amplified and inserted into pcDNA3.1(-)/HBc-linker to form pcDNA3.1(-)/TNL in which the effector molecule and the target molecule were separated by a linker sequence. pcDNA3.1(-)/TNL expression was identified by indirect immunofluorescence staining. Radioimmunoassay was used to analyse anti-HBV activity of pcDNA3.1(-)/TNL.Meanwhile, metabolism of cells was evaluated by NTT colorimetry.RESULTS: hEDN and HBVc eukaryotic fusion expression vector with a linker (Gly4Ser)3 between them was successfully constructed. pcDNA3.1(-)/TNL was expressed in HepG2.2.15 cells efficiently. A significant decrease of HBsAg concentration from pcDNA3.1(-)/TNL transfectant was observed compared to pcDNA3. 1(-)/TR (P=0.036, P<0.05).MTT assay suggested that there were no significant differences between groups (P=0.08, P>0.05).CONCLUSION: Linker introduction enhances the inhibitory effect of HBV targeted ribonuclease significantly. | Wei-Dong Gong Jun Liu Jin Ding Ya Zhao Ying-Hui Li Cai-Fang Xue Department of Pathogenic Organisms,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China | 2003 | World Journal of Gastroenterology2003，9，7： | 6 |
| 18 | Numerical analysis of carbon saving potential in a top gas recycling oxygen blast furnace显示文摘Aiming at the current characteristics of blast furnace(BF)process,carbon saving potential of blast furnace was investigated from the perspective of the relationship between degree of direct reduction and carbon consumption.A new relationship chart between carbon consumption and degree of direct reduction,which can reflect more real situation of blast furnace operation,was established.Furthermore,the carbon saving potential of hydrogen-rich oxygen blast furnace(OBF)process was analyzed.Then,the policy implications based on this relationship chart established were suggested.On this basis,the method of improving the carbon saving potential of blast furnace was recycling the top gas with removal of CO_2 and H_2O or increasing hydrogen in BF gas and full oxygen blast.The results show that the carbon saving potential in traditional blast furnace(TBF)is only 38-56kg·t^(-1) while that in OBF is 138kg·t^(-1).Theoretically,the lowest carbon consumption of OBF is 261kg·t^(-1)and the corresponding degree of direct reduction is 0.04.In addition,the theoretical lowest carbon consumption of hydrogen-rich OBF is 257kg·t^(-1).The modeling analysis can be used to estimate the carbon savings potential in new ironmaking process and its related CO_2 emissions. | Xue-feng She Xiu-wei An Jing-song Wang Qing-guo Xue Ling-tan Kong | 2017 | Journal of Iron and Steel Research(International)2017，24，6： | 5 |
| 19 | Softening and Melting Behavior of Mixed Burden for Oxygen Blast Furnace显示文摘The behaviors of mixed burden in the cohesive zone of oxygen blast furnace were studied by softening and melting tests, and the influence of reducing gas and burden basicity on the softening and melting behaviors of mixed burden was also investigated. The results indicated that the softening range became wide, however, the melting range narrowed sharply in the atmosphere of oxygen blast furnace. The permeability of burden in the oxygen blast furnace was obviously improved comparing with the conventional blast furnace. In addition, the content of sulphur in the dripping iron of oxygen blast furnace was much lower than that of conventional blast furnace, however, the content of carbon increased. An optimum basicity of burden, which could lead to the appearance of the narrower melting range and better permeability of burden, was obtained in the atmosphere of oxygen blast furnace. | AN Xiu-wei WANG Jing-song LAN Rong-zong HAN Yi-hua XUE Qing-guo | 2013 | Journal of Iron and Steel Research(International)2013，20，5： | 5 |
| 20 | Proteomic analysis of differentially expressed proteins involving in liver metastasis of human colorectal carcinoma显示文摘BACKGROUND:Early diagnosis of liver metastasis of colorectal carcinoma is very important for the appropriate treatment of such patients.However,there has been no effective approach available for clinical application.The present study aimed to investigate the differential expression of proteins in patients with liver metastasis of colorectal carcinomas using proteomic analysis and evaluate its potentiality in clinical diagnosis. METHODS:Fluorescence two-dimensional differential in-gel electrophoresis(2-D DIGE)was used to analyze and compare the protein expression between normal mucosa,the primary focus,and liver metastases.Proteomic analysis was made to identify the differentially expressed proteins.Immunohistological staining was used to confirm the expression of differentially expressed proteins in colorectal carcinomas and areas of liver metastasis. RESULTS:A 1.5-fold difference was found with 46 differentially expressed proteins.In 20 differentially expressed proteins,3 were down-regulated and 17 up-regulated in liver metastases. Proteomic analysis showed that the S-adenosylmethionine transgelin variant was down-regulated in liver metastasis tissues.Zinc finger protein 64 homolog(Zfp64),guanine nucleotide exchange factor 4(GEF4),human arginase,glutathione S-transferases(GSTs)A3,and tumor necrosis factorα(TNF-α)-induced protein 9 were up-regulated in liver metastasis tissues.Immunohistochemical staining confirmed that human arginase expression was higher in liver metastases than in the primary focus. CONCLUSIONS:There was a significant difference in protein expression between the primary focus of colorectal carcinoma and liver metastases.The differentially regulated proteins were closely related to liver metastasis of colorectal carcinoma. Elevated human arginase may be an important molecular marker for liver metastasis from colorectal carcinoma. | Li, Shi-Yong An, Ping Cai, Hui-Yun Bai, Xue Zhang, Ying-Nan Yu, Bo Zuo, Fu-Yi Chen, Gang | 2010 | Hepatobiliary & Pancreatic Diseases International2010，9，2： | 4 |
您所在的机构暂未开通此部分的功能，请联系管理员