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| 1 | Polymorphisms of MTHFD,plasma homocysteine levels, and risk of gastric cancer in ahigh-risk Chinese population显示文摘Purpose:Accumulative evidence suggests that folate has a protective effect on gastric cancer. The methylenetetrahyd-rofolate dlehydrogenase(MTHFD) plays an important role in folate and homocysteine metabolisms, and polymorphisms of MTHFD may result in disturbance of the folate-mediated homocysteine pathway. The aim of this study is to test the hypothesis that genetic variants of MTHFD and plasma homocysteine levels are associated with risk of gastric cancer and modulated by genotypes of methylenetetrahydrofolate reductase(MTHFR). Experimental Design: We genotyped G1958A and T401C in MTHFD and C677T in MTHFR and detected total plasma homocysteine(tHcy) levels in a case-control study of 589 gastric cancer cases and 635 cancer-free controls in a high-risk Chinese population. Results:The variant genotypes of MTHFD 1958AA and 401CC were associated with a significantly increased risk of gastric canceradjusted odds ratio(OR), 2.05; 95% confidence interval(95% CI),1.34-3.13 for 1958AA; adjusted OR,1.43; 95% CI,1.14-1.80 for 401CC compared with 1958GG/GA and 401TT/TC genotypes, respectively. Both of the effects were more evident in the subjects carrying MTHFR 677CT/TT genotypes. The average tHcy level was significantly higher in gastric cancer cases than in controls(P < 0.01), and the upper quartile of tHcy (> 13.6 mu mol/L) was associated with an 82% significantly increased risk of gastric cancer, compared with the lowest quartile of tHcy(<= 8.0 pmol/L;adjusted OR,1.82; 95% CI,1.20-2.75). Conclusions:The strong associations between MTHFD variants and the plasma tHcy levels and gastric cancer risk suggest, for the first time, a possible gene-environment interaction between genetic variants of folate-metabolizing genes and high tHcy levels in gastric carcinogenesis. | Wang, L. N Ke, Q Chen, W. S Wang, J. M Tan, Y. F Zhou, Y Hua, Z. L Ding, W. L Niu, J. Y Shen, J Zhang, Z. F Wang, X. R Xu, Y. H Shen, H. B Yi Xing | 2007 | 南京医科大学学报(自然科学版)2007，27，7： | 10 |
| 2 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 3 | Development of novel self-humidifying composite membranes for fuel cells显示文摘 | Liu F Q Yi Xing D M | 2003 | Power Source2003，124，： | 1 |
| 4 | Analysis of α and β-tubulin genes of Bombyx mori using an EST database显示文摘 | Hideki K Kimihiko S Guo Xing Q | 2003 | Insect Biochemy Mol Biol2003，33，： | 1 |
| 5 | Structural investigations of Fe-Ga alloys: Phase relations and magnetostrictive behavior 显示文摘 | XING Q DU Y MCQUEENEY R J LOGRASSO T A | 2008 | Acta Materialia2008，56，16： | 1 |
| 6 | Microbial community structure of ethanol type fermentation in bio-hydmgen production 显示文摘 | REN N Q XING D F ZHAO L H | 2007 | Environmental Microbiology2007，9，5： | 1 |
| 7 | Porcine TEF1 and RTEFI: Mo- lecular characterization and association analyses with growth traitsr显示文摘 | Xu X Xing S Du Z Q | 2008 | Comparative Biochemistry and Physiology Part B: Bi-ochemistry and Molecular Biology2008，150，4： | 1 |
| 8 | Comparison of survival and embryonic development inhuman ooCytes cryopreserved by slow- freezing and vitrification显示文摘 | Cao YX Xing Q Li L | 2009 | Fertil Steril2009，92，： | 1 |
| 9 | J Phys: Condens Matter显示文摘 | Sun J Yao Q Xing H | 2007 | 19:486 2152007，19，48： | 1 |
| 10 | A strip-map SARcoherent jammer structure utilizing periodic modulationtechnology 显示文摘 | Liu Q F Xing S X Wang X S | 2011 | Progress in Electromagnetics Research B PIER-B2011，28，： | 1 |
| 11 | Effect of serum creatine kinase-MBmass on the early and hierarchical diagnosis of related artery reperfusion in acute myocardial infarction 显示文摘 | Xing S S Xing Q C Zhang Y | 2007 | Postgrad Med J2007，83，3： | 1 |
| 12 | Bound-free vacuum UV emissions of (Xe Cs)+ and (Kr Cs)+ ionic excimers by relativistic electron beam excita tion显示文摘 | Wang Q Tan S Ueda K | 1997 | Appl Phys Lett1997，71，18： | 1 |
| 13 | Continuous hydrogenproduction of auto-aggregative Ethanoligenens harbinense YUAN-3 under non-sterile condition显示文摘 | Xing D F Ren N Q Wang A J | 2008 | International Journal ofHydrogen Energy2008，33，5： | 1 |
| 14 | Performance evaluation of liquid flow with NPCM particles in microchannels显示文摘 | XING K Q TAO Y -X HAO Y L | 2005 | ASME Trans C:J of Heat Transfer2005，127，8： | 1 |
| 15 | Axonal accumulation of hyperpolarization - activated cyclic nucleotide - gated cation channels contributes to mechanical allodynia after peripheral nerve injury in rat显示文摘 | JIANG Y Q XING G G WANG S L | 2008 | Pain2008，137，3： | 1 |
| 16 | The longitudinal 'trapping' phenomenon in the simultaneous acceleration of intense H ^+ and H - in an RFQ 显示文摘 | Xing Q Z Fu S N Lin Y Z | 2005 | Nuclear Instruments and Methods in Physics Research : Section A2005，538，1： | 1 |
| 17 | Exogenous neurogenesis and angiogenesis in the subventricular zone and the peri-infarction regionand improves after focal neurological function cortical infarction in hypertensive rats显示文摘 | Ling L Hou Q Xing kallikrein enhances S | 2008 | Brain Res2008，12，6： | 1 |
| 18 | Natural variation in GS5 plays an important role in regulating grain size and yield in rice显示文摘 | Li Y B Fan C C Xing Y Z Jiang Y H Luo L J Sun L Shao D Xu C J Li X H Xiao J H He Y Q Zhang Q F | 2011 | Nature genetics2011，2，43： | 1 |
| 19 | Changes in reference evapotranspiration across the Tibetan Plateau: Observations and future projections based on statistical downscaling显示文摘 | Wang W Xing W Shao Q | 2013 | Journal of Geophysical Research: Atmospheres2013，118，10： | 1 |
| 20 | Equations for the soil-water characteristic curve 显示文摘 | Fredlund D G Xing A Q | 1994 | Canadian Geotechnical Journal1994，31，4： | 1 |
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